Autologous omentum transposition for regeneration of a renal injury model in rats

Abstract Background After renal trauma, surgical treatment is vital, but sometimes there may be loss of function due to fibrosis. This study aimed to evaluate the effect of autologous omentum flaps on injured renal tissues in a rat model. Methods A total of 30 Wistar albino rats were included and randomly divided equally into a control group and four intervention groups. Iatrogenic renal injuries were repaired using a surgical technique (primary repair 1 group and primary repair 2 group) or transposition of the autologous omentum (omentum repair 1 group and omentum repair 2 group). Blood samples were taken preoperatively and on the 1st and 7th postoperative days in all groups and on the 18th postoperative day in the control and two intervention groups. All rats were sacrificed on the 7th or 18th day postoperatively, and their right kidneys were taken for histopathological evaluation. Results The mean urea level significantly decreased from day 1 to day 7 and from day 1 to day 18 in the omentum repair 2 group (P = 0.005 and P = 0.004, respectively). There were no other significant changes in urea or creatinine levels within the intervention groups (P > 0.05). There was no significant correlation between the urea and creatinine levels and the histological scores (P > 0.05). The primary repair 1 and 2 groups had significantly higher median granulation and inflammation scores in the kidney specimen than the control and omentum repair groups (P < 0.05). The omentum repair 2 group had significantly lower median granulation and inflammation scores in the surrounding tissues than the primary repair 2 group (P < 0.05). The completion score for the healing process in the kidney specimen was significantly higher in the omentum repair groups than in the primary repair groups (P < 0.05). The omentum repair 2 group had significantly lower median granulation and inflammation scores in the surrounding tissues than the primary repair 2 group (P < 0.05). Granulation degree in the kidney specimen was strongly and positively correlated with the inflammation degree (r = 0.824, P < 0.001) and foreign body reaction in the kidney specimen (r = 0.872, P < 0.001) and a strong and negative correlation with the healing process completion score in the kidney (r = − 0.627, P = 0.001). Inflammation degree in the kidney specimen was strongly and positively correlated with the foreign body reaction in the kidney specimen (r = 0.731, P = 0.001) and strongly and negatively correlated with the healing process completion score in the kidney specimen (r = − 0.608, P = 0.002). Conclusion Autologous omentum tissue for kidney injury repair attenuated inflammation and granulation. Additionally, the use of omental tissue to facilitate healing of kidney injury may theoretically lead to a more effective healing process and reduced fibrosis and tissue and function loss.

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Contribution of Autologous Omentum Transposition to the Regeneration of Renal Injuries in the Rat Model

10.21203/rs.3.rs-54600/v2 ◽

2020 ◽

Author(s):

Tayfun Bilgic ◽

Umit Ince ◽

Fehmi Narter

Keyword(s):

Rat Model ◽

Primary Repair ◽

Healing Process ◽

Histological Finding ◽

Kidney Injury ◽

Study Groups ◽

Renal Tissue ◽

Albino Rats ◽

Loss Of Function ◽

Renal Injuries

Abstract Aim: After renal trauma, surgical treatment is vital, but sometimes there may be loss of function due to fibrosis. We aimed to evaluate the repair effect of transpositioned autologous omentum on injured renal tissue in a rat model. Methods: A total of 30 female Wistar Albino rats were included and they were randomly separated into a sham group and four study groups. Iatrogenic renal injuries were repaired using a surgical technique (primary repair 1 and 2 groups) or transpositioned autologous omentum (omentum repair 1 and 2 groups). In all groups, blood samples were taken preoperatively and on the 7 th postoperative day in all groups and also on the 18 th postoperative day in the control and two study groups. All rats were sacrificed on the 7 th or 18 th day postoperatively and their right kidneys were taken for histopathological evaluation.Results: There was a trend toward decrease in urea and creatinine levels in all the groups. There was no significant correlation between urea and creatinine levels and histological finding scores. The omentum repair group had significantly lower inflammation and granulation scores compared with the primary repair and sham groups. There was a significant and positive correlation between inflammation and granulation and fibrosis scores. There was a significant and negative correlation between healing completion score and either inflammation and granulation scores. There were also positive correlations between histological findings in the kidney specimen and surrounding tissues. Conclusion: The use of the autologous omentum tissue for repair of kidney injury had attenuation effects on inflammation and granulation compared with primary repair. These results imply that use of omentum tissue to facilitate healing of kidney injury may theoretically lead to a more effective healing process and reduced fibrosis and tissue and function loss. These potentially beneficial effects of autologous omentum tissue should be investigated in further well-designed experimental and clinical studies.

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Contribution of Autologous Omentum Transposition to the Regeneration of Renal Injuries in the Rat Model

10.21203/rs.3.rs-54600/v1 ◽

2020 ◽

Author(s):

Tayfun Bilgic ◽

Umit Ince ◽

Fehmi Narter

Keyword(s):

Foreign Body ◽

Rat Model ◽

Foreign Body Reaction ◽

Surrounding Tissue ◽

Control Group ◽

Albino Rats ◽

Histopathological Analysis ◽

Kidney Surgery ◽

Omentum Transposition ◽

Renal Injuries

Abstract Aim We aimed to evaluate the repair effect of transpositioned autologous omentum on the injured renal tissues in the rat model. Methods A total of 30 female Wistar Albino rats were included and they were randomly separated into control and four groups. In all groups, blood samples were taken at preoperatively and at the 7th day postoperatively, additionally they were taken at 18th day postoperatively for creatinine and urea analysis. All rats of control group were sacrificed at 18th day postoperatively and right kidneys were taken for histopathological evaluation. After the created renal injuries were repaired primarily kidney surgery or omentum added kidney surgery. Results According to the our results, granulation and inflammation in kidney specimens were moderately and highly positive correlated with granulation, inflammation, fibrosis and foreign body reaction in surrounding tissue ((r:0.478, p = 0.008), (r:0.591, p = 0.001), (r:0.394, p = 0.031), (r:0.635, p < 0.001)). Granulation in kidney specimen was highly possitive correlated with inflammation and foreign body reaction in kidney specimens ((r:0.824, p < 0.001), (r:0.872, p < 0.001)). We could not show any significant correlation between biochemical values (U, Cr) and histopathological findings. Conclusion According to our results, our biochemical results were not correlated with histopathological analysis. But we detected histopathogical significant differences between primary kidney repair and omentum added kidney repair groups. And we recommend that kidney injuries should be surgical repaired by stem cell sources as like omentum tissue.

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Contribution of Autologous Omentum Transposition to the Regeneration of Renal Injuries in the Rat Model

10.21203/rs.3.rs-54600/v3 ◽

2020 ◽

Author(s):

Tayfun Bilgic ◽

Umit Ince ◽

Fehmi Narter

Keyword(s):

Rat Model ◽

Primary Repair ◽

Healing Process ◽

Histological Finding ◽

Kidney Injury ◽

Study Groups ◽

Renal Tissue ◽

Albino Rats ◽

Loss Of Function ◽

Renal Injuries

Abstract Aim: After renal trauma, surgical treatment is vital, but sometimes there may be loss of function due to fibrosis. We aimed to evaluate the repair effect of transpositioned autologous omentum on injured renal tissue in a rat model.Methods: A total of 30 female Wistar Albino rats were included and they were randomly separated into a sham group and four study groups. Iatrogenic renal injuries were repaired using a surgical technique (primary repair 1 and 2 groups) or transpositioned autologous omentum (omentum repair 1 and 2 groups). In all groups, blood samples were taken preoperatively and on the 7th postoperative day in all groups and also on the 18th postoperative day in the control and two study groups. All rats were sacrificed on the 7th or 18th day postoperatively and their right kidneys were taken for histopathological evaluation. Results: There was a trend toward decrease in urea and creatinine levels in all the groups. There was no significant correlation between urea and creatinine levels and histological finding scores. The omentum repair group had significantly lower inflammation and granulation scores compared with the primary repair and sham groups. There was a significant and positive correlation between inflammation and granulation and fibrosis scores. There was a significant and negative correlation between healing completion score and either inflammation and granulation scores. There were also positive correlations between histological findings in the kidney specimen and surrounding tissues. Conclusion: The use of the autologous omentum tissue for repair of kidney injury had attenuation effects on inflammation and granulation compared with primary repair. These results imply that use of omentum tissue to facilitate healing of kidney injury may theoretically lead to a more effective healing process and reduced fibrosis and tissue and function loss. These potentially beneficial effects of autologous omentum tissue should be investigated in further well-designed experimental and clinical studies.

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The Effects of Using Bioglue in Nasal Septal Surgery

Ear Nose & Throat Journal ◽

10.1177/0145561321991349 ◽

2021 ◽

pp. 014556132199134

Author(s):

Bugra Subasi ◽

Ender Guclu

Keyword(s):

Foreign Body ◽

Nasal Septum ◽

Foreign Body Reaction ◽

Cell Damage ◽

Cartilage Damage ◽

Tissue Adhesive ◽

Control Group ◽

Albino Rats ◽

Study Group ◽

Septal Surgery

Objectives: Nasal septal surgery is one of the most common surgical procedure performed by otolaryngologists. Nasal packs are used for bleeding control, prevention of septal hematoma, replacement of mucoperichondrial flaps, and stabilization of the septum after nasal septal surgery. The aim of this study was to investigate the effects of albumin-glutaraldehyde–based tissue adhesive (Bioglue), which can be used as an alternative to nasal pack on the nasal septum after experimental nasal septum surgery. Methods: A total of 16 female Wistar albino rats were randomly separated into the study group (n = 10) and the control group (n = 6). After raising the mucoperichondrial flap on one side of the septum, Bioglue was used to fix the mucoperichondrial flap over the septal cartilage ın the study group and nasal packs (Merocel) were used for fixation in the control group. The rats were sacrificed at 2 and 4 weeks after septoplasty. All the tissue samples were evaluated under light microscope by the same pathologist in respect of foreign-body reaction, degree of inflammation, granulation tissue, fibrosis, cartilage damage, and cilia and goblet cell damage. In the control group, the Merocel packs were removed after 2 days and the groups were compared in terms of hematoma. Results: No hematoma was observed in any group. Septal perforation was determined in all the study group participants and loss of cilia and goblet cells and foreign-body reaction were found in 8 samples of the study group participants and in none of the control group. Conclusions: The results of this study show that Bioglue caused segmental cartilage injury; therefore, it may not suitable for use following septal surgery.

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The Response of the Pulp-Dentine Complex, PDL, and Bone to Three Calcium Silicate-Based Cements: A Histological Study in an Animal Rat Model

Bioinorganic Chemistry and Applications ◽

10.1155/2020/9582165 ◽

2020 ◽

Vol 2020 ◽

pp. 1-13 ◽

Cited By ~ 1

Author(s):

Ranjdar Mahmood Talabani ◽

Balkees Taha Garib ◽

Reza Masaeli

Keyword(s):

Inflammatory Response ◽

Calcium Silicate ◽

Histological Study ◽

Healing Process ◽

Mineral Trioxide Aggregate ◽

Experimental Period ◽

Tissue Reaction ◽

Cortical Plate ◽

Control Group ◽

Albino Rats

Objective. The aim of this study was to histologically examine the tissue reaction of three different calcium silicate cements in the closure of perforations in rat incisor teeth. Material and Methods. An experimental lateral root perforation with pulp exposure was performed in 32 lower incisors of 16 male Wistar albino rats. They were randomly assigned into three test groups (each including eight teeth) that were filled either by Biodentine (BD) or MicroMega mineral trioxide aggregate (MM-MTA) or EndoSequence root repair material putty (ESRRM putty), besides eight unperforated incisors from the other four rats (control group). The inflammatory response and healing process were evaluated histologically and scored after one and four weeks. Differences among groups were tested by Kruskal–Wallis tests at P≤0.05. Results. In the first week, BD produced more inflammatory response in the pulpal (score 3) than other materials (score 2). Only ESRRM putty showed odontoblast-like cells in 50%, 25% dentine-like deposit, 25% evidence of bone deposition in the drilling site (score 2), and minimum periodontal ligament (PDL) necrosis and disorganization (25%, score 2). After one month, all groups had healthy pulpal tissue, but 25% of ESRRM putty retained score 1 inflammatory response, and 50% of the BD case had an incomplete palisading odontoblast layer (score 3). A thick and regular dentine bridge deposition was seen in the ESRRM putty group in comparison with MM-MTA and BD cases. The cortical plate healing in all ESRRM putty samples was complete (score 3), while an incomplete closure was seen in MM-MTA and BD groups (score 2). Both the MM-MTA and ESRRM putty groups had fully organized PDL (score 2), while in 50% of BD cases, a necrotizing area and disorganized PDL with inflammatory cells infiltration were still present. Statistically significant differences in the scores of any histologic parameters among the three tested materials were observed neither in the 1st nor in the 4th weeks of the experimental period. Conclusion. Better tissue compatibility and repair of pulpal and periodontal tissue have been detected after lateral perforation in the root of rat incisors when treated with ESRRM putty than MM-MTA and BD. However, the difference was not significant.

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The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model

International Journal of Endocrinology ◽

10.1155/2013/313528 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 18

Author(s):

Ender Hur ◽

Alev Garip ◽

Asuman Camyar ◽

Sibel Ilgun ◽

Melih Ozisik ◽

...

Keyword(s):

Vitamin D ◽

Acute Kidney Injury ◽

Rat Model ◽

Urine Volume ◽

Kidney Injury ◽

Control Group ◽

Albino Rats ◽

Tubular Injury ◽

Renal Histology ◽

Experimental Rat Model

Introduction. Acute kidney injury (AKI) pathogenesis is complex. Findings of gentamicin nephrotoxicity are seen in 30% of the AKI patients. Vitamin D has proven to be effective on renin expression, inflammatory response, oxidative stress, apoptosis, and atherosclerosis. We aimed to investigate the effect of vitamin D in an experimental rat model of gentamicin-induced AKI.Methods. Thirty nonuremic Wistar albino rats were divided into 3 groups: Control group, 1 mL saline intramuscular (im) daily; Genta group, gentamicin 100 mg/kg/day (im); Genta + vitamin D, gentamicin 100 mg/kg/day (im) in addition to 1α, 25 (OH)2D30.4 mcg/kg/day subcutaneously for 8 days. Blood pressures and 24-hour urine were measured. Blood urea and creatinine levels and urine tubular injury markers were measured. Renal histology was semiquantitatively assessed.Results. Urea, creatinine and urine neutrophil gelatinase-associated lipocalin, and kidney injury molecule-1 were all increased in Genta group indicating AKI model. Systolic blood pressure decreased, but urine volume and glutathione increased in Genta + Vit D group compared to Control group. Histological scores indicating tubular injury increased in Genta and Genta + Vit D groups.Conclusions. Vitamin D does not seem to be effective on histological findings although it has some beneficial effects via RAS system and a promising effect on antioxidant system.

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Histopathological effects of fibrin glue on penile fracture in a rat model

Clinical & Investigative Medicine ◽

10.25011/cim.v34i6.15893 ◽

2011 ◽

Vol 34 (6) ◽

pp. 336 ◽

Cited By ~ 1

Author(s):

Cemal Tasdemir ◽

Emine T Samdanci ◽

Muhammet G Turtay ◽

Cemal Firat ◽

Hakan Oguzturk ◽

...

Keyword(s):

Fibrin Glue ◽

Primary Repair ◽

Dorsal Side ◽

Control Group ◽

Albino Rats ◽

Penile Fracture ◽

Tissue Healing ◽

Significant Difference ◽

Cavernous Tissue ◽

Histopathological Effects

Purpose: To evaluate both histopathological effects and potential clinical application of fibrin glue on the penile cavernosal tissue. Methods: Experimental penile fracture was formed by incising from the proximal dorsal side of the penis in 32 Wistar Albino rats. The rats were randomly assigned to four main groups of eight animals each. In the control group, the incision was not repaired and it was left to secondary healing. In the primary repair group, the incision was primarily repaired. In the fibrin glue group, glue was applied only to the incision. In the final group, fibrin glue was applied to the incision following primary repair. Three weeks later, penectomy tissue was examined histopathologically. Results: When the control group was compared with primary repair+fibrin glue group, the differences in cavernous tissue healing with fibrosis and inflammation were statistically significant (p = 0.04 and 0.01, respectively). The primary repair+fibrin glue group, showed the best cavernous healing with fibrosis observed in only one rat. . There was no significant difference between the control group and the other groups according to cavernous tissue healing with fibrosis and inflammation (p = 0.11 and 0.12). Hyperemia was observed in the all groups of rats. Conclusions: Fibrin glue can be used in cavernoseal surgeries due to its adhesive and potentially anti-inflammatory features.

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Ellagic Acid Activity in Healing Process of Incision Wound on Male Albino Rats (Rattus norvegicus)

KnE Life Sciences ◽

10.18502/kls.v3i6.1131 ◽

2017 ◽

Vol 3 (6) ◽

pp. 224

Author(s):

Hardany Primarizky ◽

Wiwik Misaco Yuniarti ◽

Bambang Sektiari Lukiswanto

Keyword(s):

Wound Healing ◽

Treatment Group ◽

Ellagic Acid ◽

Healing Process ◽

Control Group ◽

Albino Rats ◽

Wound Healing Process ◽

Treatment Groups ◽

Significant Difference ◽

Incision Wound

This study was conducted to prove whether ellagic acid can be used as a regulator of incision wound healing process in male albino rats as experimental animals. A total of 24 male healthy rats based on physical examination, aged at 3 months old and weight at 150-180 grams used in this study. Rats were divided into three groups and each group has 8 replications, group of control (P0) has no incision wound and no medication, treatment group (P1) has incision wound and treated with Betadine® and (P2) has incision wound and treated with 2,5 % ellagic acid. The variable that observed are histopathology changes (collagen, PMN, angiogenesis and fibrosis) from the male albino rats. The samples collection was taken at day 15 and data were collected and analyzed by Analysis of Variance (ANOVA) test. The result of collagen and PMN cells score in the control group (P0) were 1.80 ± 0.16 and 1.6 ± 0.08 with mild angiogenesis and no fibrosis. The results in the treatment (P2) group, where the collagen and PMN scores were 1.60 ± 0.08 and 1.5 ± 0.8, accompanied by mild angiogenesis and fibrosis. Meanwhile, in the treatment (P1) group, the results of scoring of collagen and PMN were lower than the other groups, 0, 80 ± 0.08 and 0.6 ± 0.16 accompanied by moderate angiogenesis and fibrosis. There was a significant difference of collagen scoring, PMN cells number, fibrosis and angiogenesis among the treatment groups (p<0.05). P1 treatment group has significant different with treatment groups P0 and P2 (p<0.05), but there is no significant difference between treatment groups P0 and P2 (p>0.05). From the results, it can be concluded that the topical application of ellagic acid ointment was proved to provide healing process on incision wound. Topical administration of ellagic acid ointment for 14 days was able to improve the skin condition and promoted the wound healing.Keywords: Ellagic acid ; wound healing ; collagen ; angiogenesis ; fibrosis ; anti-inflammatory ; antioxidant ; antibacterial.

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Histological Observation Related to the Use of Laser and Ultrasound on Bone Fracture Healing

IIUM Medical Journal Malaysia ◽

10.31436/imjm.v10i2.679 ◽

2020 ◽

Vol 10 (2) ◽

Author(s):

Al-Habib MF ◽

Salman MO ◽

Faleh FW ◽

Al-Ani IM

Keyword(s):

Fracture Healing ◽

Bone Fracture ◽

Nd:Yag Laser ◽

Healing Process ◽

Complete Healing ◽

Control Group ◽

Albino Rats ◽

Bone Fracture Healing ◽

Partial Fracture ◽

Haversian System

Objective: To study the effect of both laser and ultrasound radiation on bone fracture healing process. Materials and Methods: Nd:YAG laser (1064 nm wavelength, 135 mW power, 16 joules energy) and ultrasound (1 MHz frequency, 50 mW/cm2 power intensity) were used in this work. Fifteen mature, male, albino rats, were divided into three groups and subjected to a partial fracture on the lateral aspect of femur by a sharp blade. The fi rst group of these animals served as control group. The second group was illuminated by the Nd:YAG laser for two minutes; the fi rst dose was given immediately after surgical fracture induction; the other doses were given on days two, three, six and then one dose weekly for the next three weeks while the third group were treated by the addition of the CW ultrasound perpendicular to the laser treatment in the second group. Results: The present study showed that ultrasound increases the penetration of laser power through the tissue. The histological assessments at day 28 after the fracture of fi rst group showed incomplete healing of the bone with disfi guration and disarrangement of Haversian system and the periosteum was not yet well developed. Treatment with laser showed irregularity and lack of Haversian system formation in bone healing of the second group. The laser and ultrasound treated group (third group) expressed a complete healing at the site of fracture with a complete layer of periosteum and a well arranged Haversian system. Conclusion: Combination of laser and ultrasound in therapy can enhance healing process of a fractured bone more than laser therapy alone, as ultrasound increases the depth of laser penetration in tissue.

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Melatonin mitigates cisplatin-induced acute kidney injury through regulation of the heat shock proteins expressions

10.21203/rs.3.rs-703570/v1 ◽

2021 ◽

Author(s):

Ali Tuğrul Akin ◽

Murat Unsal ◽

Tayfun Ceylan ◽

Emin Kaymak ◽

Emel Ozturk ◽

...

Keyword(s):

Acute Kidney Injury ◽

Uric Acid ◽

Heat Shock ◽

Heat Shock Proteins ◽

Blood Serum ◽

Renal Cortex ◽

Kidney Injury ◽

Control Group ◽

Albino Rats ◽

Shock Proteins

Abstract Purpose. To determine the protective effects of melatonin (MEL) in acute kidney injury (AKI) induced by Cisplatin (CP), widely used as a chemotherapeutic in the treatment of many cancer types, via assessment of heat-shock proteins (HSPs) levels in rats. Methods. Total 40 Wistar albino rats were divided into four groups: Control (n = 10), MEL (n = 10, 10 mg/kg/i.p melatonin for 8 days), CP (n = 10, 7 mg/kg/i.p cisplatin at the 5th day), and CP + MEL (n = 10, 10 mg/kg/i.p melatonin for 8 days and 7 mg/kg/i.p cisplatin at the 5th day). After administrations, animals were sacrificed, and kidney tissues were extracted. Histopathological changes were evaluated and glomerular and tubular immunoreactivities of HSP47, HSP60, HSP7, and HSP90 in renal cortex were detected by immunohistochemistry. Moreover, blood serum BUN, creatinine and uric acid levels were measured to assess of kidney function. Results. CP group showed histopathological deterioration compared to Control group and MEL treatment attenuated this damage. When compared with Control and MEL groups, an increase in HSPs immunoreactivities in renal cortex and blood serum BUN, creatinine and uric acid levels were observed in the CP group. Furthermore, an improvement was observed in the CP + MEL in terms of these parameters compared to the CP group. Conclusion. According to our results, MEL could exert a significant protective effect to ameliorate CP-induced AKI via regulation of heat-shock protein expressions.

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