scholarly journals Human acellular amniotic membrane implantation for lower third nasal reconstruction: a promising therapy to promote wound healing

2018 ◽  
Vol 6 ◽  
Author(s):  
Si-Liang Xue ◽  
Kai Liu ◽  
Ornella Parolini ◽  
Yue Wang ◽  
Li Deng ◽  
...  
Keyword(s):  
Wound Healing ◽  
Amniotic Membrane ◽  
Skin Lesions ◽  
Control Group ◽  
Nasal Reconstruction ◽  
Nasal Tissue ◽  
Alternative Approach ◽  
The Face ◽  
Duration Of Operation ◽  
Reconstructive Techniques

Abstract Background The lower third of the nose is one of the most important cosmetic units of the face, and its reconstructive techniques remain a big challenge. As an alternative approach to repair or regenerate the nasal tissue, the biomaterial-based strategy has been extensively investigated. The aim of this study is to determine the safety and efficacy of human acellular amniotic membrane (HAAM) to repair the full-thickness defects in the lower third of the nose in humans. Methods In this study, 180 patients who underwent excision of skin lesions of the lower third of the nose from 2012 to 2016 were included; of the patients, 92 received HAAM and Vaseline gauze treatments, and the other 88 patients received Vaseline gauze treatment only. The haemostasis time and the duration of operation were recorded during surgery; after surgery, the time to pain disappearance, scab formation and wound healing, and the wound healing rate were measured. Results Immediately after the HAAM implantation, a reduction of the haemostasis time and an accelerated disappearance of pain were observed. Compared with the control group, the formation and detachment of scab in patients who received the HAAM implantation were notably accelerated, postoperatively. When the diameter of the lesion exceeded 5 mm, the HAAM implantation was found to enhance the wound healing, although this enhancement was not seen when the diameter was less than 5 mm. Additionally, the HAAM implantation significantly reduced bleeding, wound infection and scar formation, postoperatively. Conclusions HAAM-assisted healing is a promising therapy for lower third nasal reconstruction leading to rapid wound healing and fewer complications and thus has considerable potential for extensive clinical application in repairing skin wounds. Trial registration ChiCTR1800017618, retrospectively registered on July 08, 2018.

PENGGUNAAN SELAPUT AMNION SEGAR PADA INSISI LUKA OPERASI SEKSIO SESAREA

2019 ◽  
Vol 1 (2) ◽  
pp. 47-55
Author(s):  
Syahredi Syaiful Adnani ◽  
Hafni Bachtiar
Keyword(s):  
Wound Healing ◽  
Caesarean Section ◽  
Amniotic Membrane ◽  
Healing Time ◽  
Cost Of Care ◽  
Control Group ◽  
P Value ◽  
Control Groups ◽  
And Control ◽  
Wound Healing Time

In the last few decades, the incidence of caesarean section is increasing in the world, especially in Indonesia. One of the way to treat tissue scar is through biologic and synthetic dressing where nowadays, amnion has been used as biologic dressing frequently. This study was conducted to determine the effect of the use of fresh amniotic membrane on wound incision Caesarean section compared with Caesarean section incision wound covered using regular gauze bandages and fixated with plaster in RS. Dr. Reksodiwiryo Padang. The design of this study is an experimen-tal study with Post test design with control group design. Sampling was done using a formula consecutive sampling two different test samples obtained an average of 72 people for each group. The analysis used include univariate and bivariate analyzes. The average wound healing time the difference was statistically significant (p value <0.05) in the treatment and control groups. There was highly significant difference in the proportion of local infection on day 3 between the treatment and control groups (p value <0.05). There were very significant differences in the proportion of local allergic reactions at day 3, and 5 between the treatment and control group (p <0.05). There are significant differences in terms of the cost of care per day between treatment and control groups (p <0.05). From this study, the average wound healing time has a very significant difference.Keywords: Fresh Amniotic Membrane, Wound Cesarean Section, Wound Healing

Biological effects of amniotic membrane on diabetic foot wounds: a systematic review

2018 ◽  
Vol 27 (Sup2) ◽  
pp. S19-S25 ◽  
Author(s):  
André Oliveira Paggiaro ◽  
Andriws Garcia Menezes ◽  
Alexandra Donizetti Ferrassi ◽  
Viviane Fernandes De Carvalho ◽  
Rolf Gemperli
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Amniotic Membrane ◽  
Diabetic Foot ◽  
Biological Effects ◽  
Meta Analysis ◽  
Healing Process ◽  
Intervention Group ◽  
Risk Of Bias ◽  
Control Group

Objective: The amniotic membrane has biological properties that are beneficial to the wound healing process of diabetic foot ulcers (DFU). Our aim is to analyse the scientific evidence found in literature on the use of the amniotic membrane to stimulate DFU healing. Method: A systematic review of amniotic membrane's influence was undertaken, using the search terms ‘placenta’ ‘diabetic foot’ ‘amnion’ and biological dressing’, assessing the outcomes ‘wound healing’ and ‘wound healing time’, in DFU. Following the inclusion and exclusion criteria, randomised controlled trials (RCT) were identified, and the risk of bias was analysed according to the Cochrane risk of bias tool. We conducted a meta-analysis of the two outcomes to evaluate the level of evidence. Results: We identified six clinical trials, with a total of 331 patients. The most common risks of bias in the studies were selection, attrition, and detection biases. From the meta-analysis, the difference of the intervention group (amnion) in relation to the control group was statistically significant. We found that wound healing in the group treated with amniotic membrane occurs 2.32 times more often and is 32 days faster in comparison with the group that used conventional dressings. Conclusion: There is statistical evidence to support the effectiveness of amniotic membrane in comparison with other conventional dressings. In addition, there is a clear tendency for the use of amniotic membrane treatment to result in a larger number of DFUs healing at a quicker rate.

scholarly journals Healing Effects of Human Amniotic Membrane and Burned Wool on the Second-degree Burn in Rats

2020 ◽  
Vol 9 ◽  
pp. 1759
Author(s):  
Nader Tanideh ◽  
Farideh Keshavarzi ◽  
Aida Hemat Zadeh ◽  
Sajad Daneshi ◽  
Omid Koohi-Hosseinabadi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Amniotic Membrane ◽  
Granulation Tissue ◽  
Male Rats ◽  
Control Group ◽  
Human Amniotic Membrane ◽  
Tissue Formation ◽  
Granulation Tissue Formation ◽  
Degree Burn ◽  
Second Degree

ackground: This study aimed to compare sheep burnt wool and human amniotic membrane (AM) on second-degree burn wound healing in rats. Materials and Methods: Seventy-two adult male rats of Sprague Dawley underwent general anesthesia, and a deep second-degree burn was created on their skin by a hot iron plate. Afterward, human AM, silver sulfadiazine ointment (SSD), and sheep burned wool were used on wound area for burn treatment. On days 7, 14, and 21 of the experiment, the rats were sacrificed, and histopathological assessments were done. Results: Human AM, in comparison with other groups, significantly (P<0.05) showed better improvement in all pathologic variables. Burned wool showed significant improvement compared to the control group on day 7 in the angiogenesis, on day 14 in granulation tissue formation and epithelial formation, and on day 21 in new epithelial formation (P<0.05). Burned wool compared with SSD ointment in granulation tissue formation improved significantly (P<0.05) on days 7 and 14. Also, SSD ointment in comparison with the control group significantly improved (P<0.05) granulation tissue formation and macrophage on day 7. Conclusion: Human AM has a significant effect on the treatment of second-degree burn. Burned wool has a better effect on wound healing than SSD ointment and negative control group without treatment in terms of granulation tissue and epithelium formation. [GMJ.2020;9:e1759]

scholarly journals CICATRIZAÇÃO CUTÂNEA POR SEGUNDA INTENÇÃO EM EQÜINOS TRATADOS COM VEDAPROFENO

2004 ◽  
Vol 9 (1) ◽  
Author(s):  
C.A. HUSSNI ◽  
C.G. GIANINI ◽  
A.L.G. ALVES ◽  
J.L.M. NICOLETTI ◽  
A. THOMASSIAN ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Lesions ◽  
Skin Wound ◽  
Post Treatment ◽  
Control Group ◽  
Control Groups ◽  
Skin Wound Healing ◽  
Total Period ◽  
Histopathological Studies ◽  
Macroscopic Examination

Com o objetivo de avaliar-se possíveis efeitos do vedaprofeno sobre a cicatrização de pele em eqüinos, realizaram-se lesões circulares de pele padronizadas com “punch” de 2 cm, nas regiões lombares direita e esquerda de 14 eqüinos. Estes dois últimos foram divididos em dois grupos, os quais receberam tratamento local diário com líquido de Dakin. Sete animais foram tratados com 2 mg/kg de vedaprofeno (grupo vedaprofeno) via oral, duas vezes ao dia, durante dez dias e, sete animais do grupo controle não receberam tratamento. Em todos os cavalos, as lesões do lado direito foram avaliadas macroscopicamente e por planimetria a cada seis dias e, as feridas do lado esquerdo foram analisadas por material para histopatologia no sexto e 15º dias de tratamento. Comparativamente, as lesões dos animais tratados com vedaprofeno mostraram-se secas e sem edema perilesional e as feridas controle apresentaram maior retração centrípeta. O tempo total de cicatrização não diferiu, variando entre 28 e 39 dias (média 32,86) nos animais do grupo vedaprofeno e entre 26 e 39 dias nos animais do grupo controle (média 31,71). Nos exames histopatológicos ocorreu diferença entre os grupos quanto à epitelização presente observada com maior freqüência no grupo controle. O vedaprofeno diminuiu a retração e epitelização da ferida com redução da fase exsudativa e do edema, entretanto, sem interferir no tempo total de cicatrização. Second intention skin wound healing in equines under vedaprofen treatment Abstract The aim of this study was to evaluate the effects vedaprofen of on equine skin wound healing. Circular skin lesions using a 2 cm punch were induced on right and left lumbar regions in 14 equines. The animals were divided into two groups and the wounds received daily topic treatment with Dakin solution. Seven horses (vedaprofen group) were treated with vedaprofen (2mg/kg PO BID) for 10 days, and seven (control group) were not treated. All animals had right side wounds evaluated by macroscopic examination and planimetry each 6 days and the left side wounds were submitted to histopathological studies at the 6th and 15th post-treatment days. The wounds of vedaprofene group showed drier lesions without edema when compared to the control groups wounds, which displayed more centripetal retraction.The total healing period was similar in both groups, with 28 and 39 days (average 32.86) in the vedaprofen group and 26 and 39 days (average 31.71) in the control group. The epithelial recovery was evaluated by histopathological examinations and was more often seen in the control group. The vedaprofen diminished the retraction and epithelial recovery of the wound, but did not interfere in the total period of healing with reduction of exsudative phase and edema.

scholarly journals Using Dry Human Amniotic Membrane in Secondary Intention Wound Healing After Urological Cancer Surgery: The First Randomized Clinical Trial in Iran

2021 ◽  
Vol 14 (5) ◽  
Author(s):  
Ramin Hamidi ◽  
Ebrahim Hazrati ◽  
Davood Abbasi Azar ◽  
Farzad Allameh ◽  
Babak Javanmard ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Wound Healing ◽  
Randomized Clinical Trial ◽  
Amniotic Membrane ◽  
Cancer Surgery ◽  
Control Group ◽  
Case Group ◽  
Human Amniotic Membrane ◽  
Urological Cancer ◽  
Secondary Intention

Background: Wound healing is a complex process and in some patients, it is a long process. Due to the presence of various underlying diseases in patients with cancer, this process is delayed, and as a result, the rate of complications increases. Therefore, some materials are needed to accelerate wound healing. Nowadays efficacy of dry human amniotic membrane in burn wounds and chronic wounds is proven; however, no studies have been performed on the effect of this tissue in the treatment of surgical wounds. Objectives: This study aimed at evaluating the efficacy of dry human amniotic membrane in the secondary repair of urological cancer surgery wounds. Methods: In this randomized clinical trial from January 2018 to June 2020, 40 patients who underwent urological cancer surgery and secondary intention wound healing were selected and randomly divided into 2 groups of 20 patients. In the case group, the dry human amniotic membrane was used for dressing wounds, and in the control group, normal dressing without an amniotic membrane was performed. Complications such as infection, sepsis, and reoperation, as well as the duration of wound healing and hospitalization of patients at intervals of 1 week, 1, and 3 months, were compared between the 2 groups. Results: The 2 groups matched in terms of gender, age, and wound location. The mean duration of hospitalization and wound healing was significantly lower in the case group (P < 0.05). The rate of complications was higher in the control group but was not significant. Conclusions: Dry human amniotic membranes due to the accelerated wound healing process and fewer complications could be a good choice for secondary healing of wounds after urological cancer surgery.

Biological effects of amniotic membrane on diabetic foot wounds: a systematic review

2018 ◽  
Vol 27 (Sup2) ◽  
pp. S19-S25
Author(s):  
André Oliveira Paggiaro ◽  
Andriws Garcia Menezes ◽  
Alexandra Donizetti Ferrassi ◽  
Viviane Fernandes De Carvalho ◽  
Rolf Gemperli
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Amniotic Membrane ◽  
Diabetic Foot ◽  
Biological Effects ◽  
Meta Analysis ◽  
Healing Process ◽  
Intervention Group ◽  
Risk Of Bias ◽  
Control Group

Objective: The amniotic membrane has biological properties that are beneficial to the wound healing process of diabetic foot ulcers (DFU). Our aim is to analyse the scientific evidence found in literature on the use of the amniotic membrane to stimulate DFU healing. Method: A systematic review of amniotic membrane's influence was undertaken, using the search terms ‘placenta’ ‘diabetic foot’ ‘amnion’ and biological dressing’, assessing the outcomes ‘wound healing’ and ‘wound healing time’, in DFU. Following the inclusion and exclusion criteria, randomised controlled trials (RCT) were identified, and the risk of bias was analysed according to the Cochrane risk of bias tool. We conducted a meta-analysis of the two outcomes to evaluate the level of evidence. Results: We identified six clinical trials, with a total of 331 patients. The most common risks of bias in the studies were selection, attrition, and detection biases. From the meta-analysis, the difference of the intervention group (amnion) in relation to the control group was statistically significant. We found that wound healing in the group treated with amniotic membrane occurs 2.32 times more often and is 32 days faster in comparison with the group that used conventional dressings. Conclusion: There is statistical evidence to support the effectiveness of amniotic membrane in comparison with other conventional dressings. In addition, there is a clear tendency for the use of amniotic membrane treatment to result in a larger number of DFUs healing at a quicker rate.

Efficacy of Botulinum Toxin Type A in Improving Scar Quality and Wound Healing: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

2019 ◽  
Vol 40 (5) ◽  
pp. NP273-NP285 ◽  
Author(s):  
Xiaoshuang Guo ◽  
Guodong Song ◽  
Dong Zhang ◽  
Xiaolei Jin
Keyword(s):  
Wound Healing ◽  
Botulinum Toxin ◽  
Adverse Events ◽  
Meta Analysis ◽  
Botulinum Toxin Type A ◽  
Type A ◽  
Botulinum Toxin Type ◽  
Control Group ◽  
The Face ◽  
Scar Quality

Abstract Background Botulinum toxin type A-induced “chemoimmobilization” has long been utilized for improved scar quality and wound healing; however, current evidence is limited to small studies, and evidence-based information is inadequate to make well-informed decisions. Objectives The purpose of this study was to evaluate the efficacy of botulinum toxin type A (BTA) to improve scars and wound healing. Methods The authors searched databases, including Pubmed, Embase, and Cochrane Library, to identify randomized clinical trials (RCTs) that compared outcomes of surgical scars and wounds treated with BTA vs those treated with blank or placebo controls. The Visual Analog Scale, Vancouver Scar Score, scar width, and reported patient satisfaction were utilized in evaluating outcomes. Adverse events were also recorded. Results Eleven RCTs involved a total of 486 cases (374 patients). Quantitative synthesis suggested that compared with the control group, patients in the BTA treatment group had significantly higher Visual Analog Scale scores (mean difference [MD] = 1.30, 95% confidence interval [CI]: 1.05 to 1.55), lower Vancouver Scar Scores (MD = −1.62, 95% CI: −2.49 to −0.75, P = 0.0003), and thinner scars (MD = −0.15, 95% CI: −0.20 to −0.11, P &lt; 0.00001). Patient satisfaction was higher in the BTA group than in the control group (risk ratio: 1.25, 95% CI: 1.06 to 1.49, P = 0.01). Trivial adverse events were reported. Conclusions This meta-analysis of RCTs provides reliable evidence that BTA injection is superior to placebo or blank control group in improving scar quality and wound healing in the face and neck for Asians, and negative outcomes for BTA treatment in these patients include only trivial adverse events. However, inadequate evidence supports utilization of BTA in Caucasians for primary surgical scars or for scars in locations other than the face and neck. Further studies on the standardized injection regimen and technique of BTA are warranted for clinical practice. Level of Evidence: 1

scholarly journals Transplantation of Amniotic Membrane to the Subretinal Space in Pigs

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Jens Folke Kiilgaard ◽  
Erik Scherfig ◽  
Jan Ulrik Prause ◽  
Morten la Cour
Keyword(s):  
Wound Healing ◽  
Amniotic Membrane ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Mechanical Damage ◽  
Choroidal Neovascularisation ◽  
Subretinal Space ◽  
Control Group ◽  
Surgical Removal ◽  
Complete Coverage

Purpose. To investigate the effect of transplanted amniotic membrane (AM) on subretinal wound healing.Methods. Nine Danish Landrace pigs had surgical removal of retinal pigment epithelium (RPE) and mechanical damage of Bruch’s membrane (BM) and served as a control group. 15 pigs additionally had AM transplanted to the subretinal space.Results. AM significantly reduces choroidal neovascularisation when complete coverage of the induced defect is obtained (7 pigs) (P<0.05). In cases where AM did not cover the rupture in BM choroidal tissue covered the transplanted membrane (8 pigs). AM is well tolerated in the subretinal space, causes only limited inflammation, and is covered with a monolayer of pigmented cells when in contact with the host RPE.Conclusions. AM modifies choroidal neovascularisation after BM damage and may serve as a basement membrane substitute for the RPE.

The Performance of the Cold Laser Therapy (GaAlAs-980nm)

2021 ◽  
Vol 15 (10) ◽  
pp. 3096-3102
Author(s):  
Zahra Al- Timimi
Keyword(s):  
Wound Healing ◽  
Laser Treatment ◽  
Laser Therapy ◽  
Interleukin 1 ◽  
Healing Time ◽  
Control Group ◽  
Laboratory Mice ◽  
Spot Area ◽  
The Face ◽  
Set Up

Cold laser therapy has been largely utilized to enhance wound healing caused by several bio stimulatory characteristics conferred via laser rays obviously capable towards stimulate the restoration of flexible tissues wounds. Notwithstanding, the performance of pro-inflammatory interleukins has not been investigated yet. Interleukin-1 beta (IL-1β) represents an individual of largely imperative proinflammatory cytokines, which could be concerned in wounds therapeutic. The target of this work was to investigate the influence of a 980nm laser on the IL-1β expression and its secretion in wound healing in laboratory mice. Wounds with a standard-sized of 2cm have been carried out on the face of forty-laboratory mice. Animals have been divided into two groups; half of them undergo cold laser treatment at 980 nm, continuous illuminations, output power 5 W, beam spot area at the target had 0.7 cm2, and an energy density had 643 J/cm2 delivered without delay after wounds procedure. Furthermore, the other half of the animals had been set up as a control group. Next, the animals have been divided into four groups interconnected to the healing time intervals. A repairing operation area has been uprooting and further stained by the immunohistochemistry method towards identifying the appearance of (IL-1β). Cold laser therapy has proficient to amplify the appearance of (IL-1β) near the beginning of healing stages plus boosting epithelialization remodeling procedure within one to two weeks of healing time. Cold laser therapy tested in this work resulted in enlarged expressions of (IL-1β) in the laser treatment group considerably next to one week of healing time, which has an effect on wound healing. Keywords: Laser, wounds, therapy, photobiomodulation, healing, interleukins

scholarly journals Effects of Cryopreserved Amniotic Membrane Allograft on Total Ankle Arthroplasty Wound Healing

2017 ◽  
Vol 2 (3) ◽  
pp. 2473011417S0000
Author(s):  
Thomas Bemenderfer ◽  
Robert Anderson ◽  
Susan Odum ◽  
W. Hodges Davis
Keyword(s):  
Wound Healing ◽  
Soft Tissue ◽  
Amniotic Membrane ◽  
Local Application ◽  
Control Group ◽  
Skin Closure ◽  
Ankle Arthritis ◽  
History Of ◽  
Skin Healing ◽  
Coronal Correction

Category: Ankle, Ankle Arthritis, Basic Sciences/Biologics Introduction/Purpose: Despite improvements in newer-generation total ankle arthroplasty (TAA) implants, relatively high wound-healing complication rates continue to be reported with the anterior ankle incision. Only 66% heal without wound-healing complications, 25% have minor complications requiring local care and/or oral antibiotics, and 9% experience major complications requiring reoperation (Raikin et al., 2010). Recently, multiple regenerative adjuncts have been investigated to reduce postoperative complications by enhancing local healing factors and reducing risk of infection. The relatively novel use of adjunctive therapy utilizing cryopreserved amniotic membrane modulate wound healing by down-regulating inflammation and scar formation (Hanselman et al., 2015). The purpose of our study is to determine whether the local application of cryopreserved amniotic membrane wound allograft may enhance soft tissue wound healing of the TAA anterior ankle incision. Methods: Patients with symptomatic ankle arthritis who failed conservative management underwent TAA by two senior foot and ankle surgeons at single tertiary hospital. Both senior surgeons were present and involved in all surgeries, and all patients underwent the same procedure as indicated by their pathology, postoperative regimen, and rehabilitation protocol. At skin closure, patients were either allocated to the treatment or control group strictly by the designated primary attending. The skin closure of the treatment group was performed in standard fashion with local application of cryopreserved amniotic membrane to the extensor retinacular layer and no allograft was used for the control group. Demographics, sagittal and coronal correction, and patient comorbidity information was collected. The primary outcome was time to skin healing as determined by suture removal and surgical site skin apposition without evidence of granulation tissue or eschar. Secondary outcomes were skin dehiscence, local wound care, and use of antibiotics. Results: Local application of amniotic membrane allograft significantly decreased overall time to skin healing (40 days to 28.5 days, p=0.0377). There were no reoperations for wound complications in either group. However, there was a trend in decreased dehiscence (13% to 6%, p=0.29) and antibiotic prescription (23% to 9%, p=0.09). There was no significant difference in treatment versus control group with respect to body mass index, sagittal or coronal correction, sex, history of smoking, prior arthrodesis, or primary or revision. There was a significantly higher percentage of patients with history of diabetes who received amniotic membrane than those who did not receive the adjunct therapy (20% versus 2%, p=0.01). Conclusion: Regenerative technology using local application of cryopreserved amniotic membrane allograft may enhance TAA outcomes by decreasing time to healing. Although there was a trend in decreased dehiscence and antibiotic usage, larger randomized controlled trials are necessary to determine whether local application of cryopreserved amniotic membrane allograft may enhance soft tissue wound healing and ultimately reduce the incidence of devastating soft tissue complications.

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