Bio-efficacy of entomopathogenic nematodes, Steinernema feltiae and Heterorhabditis bacteriophora against the Cabbage butterfly (Pieris brassicae [L.]) under laboratory conditions

Abstract Background Entomopathogenic nematodes (EPNs) have the potential to supersede larvicidal activity for the management of various insect pests. Result Lab experiments were conducted to test the pathogenicity of 2 EPNs local species; Steinernema feltiae and Heterorhabditis bacteriophora at different (IJs/cm2) concentrations against the cabbage butterfly, Pieris brassicae (L.). The native isolate was obtained from soil samples, collected from Rajgarh, Hamachi Pradesh, India. Petri dish bioassay used the EPNs species (S. feltiae HR1 and H. bacteriophora HR2) at the concentrations (0, 10, 20, 40, 80, 160 IJs/cm2). Based on the pathogenicity of the strains, only 2 isolates effectively showed larvicidal activity. The highest (%) (72.08 and 67.42%), at the 2nd instar larval mortality was recorded in the treatments with H. bacteriophora and S. feltiae at160 IJs/cm2, respectively. At the 4th instar larvae, respective larval mortality (85.38, 69.50%) was recorded in treatment with H. bacteriophora, and S. feltiae, respectively, at160 IJs/cm2. In case of pupae, the mortality rates were (62.12, 58.58%) for H. bacteriophora and S. feltiae, respectively, at 160 IJs/cm2; (74 and 12%) for both the tested EPNs, respectively, at 80 IJs/cm2. Percent of P. brassicae larval mortality treated with the tested EPN isolates was significantly higher than the untreated control. Results revealed that the percent of larval mortality significantly increased with the increase in time periods, being maximum at 72 h. S. feltiae and H. bacteriophora, strains showed potent larvicidal activity at low concentration even at 48 and 72 h of exposure. Conclusion This study revealed that the local strains of EPNs (S. feltiae HR1 and H. bacteriophora HR2) were found as a biocontrol agent against P. brassicae.

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Bio-efﬁcacy of entomopathogenic nematodes, Steinernema feltiae and Heterorhabditis bacteriophora against the Cabbage butterfly Pieris brassicae (L.), under laboratory conditions

10.21203/rs.3.rs-304688/v1 ◽

2021 ◽

Author(s):

Kasi Indra Kumar ◽

Mohinder Singh ◽

Kanchhi Maya Waiba ◽

Sharma Monika ◽

MA Waseem ◽

...

Keyword(s):

Larvicidal Activity ◽

Entomopathogenic Nematodes ◽

Insect Pests ◽

Larval Mortality ◽

Petri Dish ◽

Pieris Brassicae ◽

Steinernema Feltiae ◽

Pupal Development ◽

Cabbage Butterfly ◽

Cabbage Pests

Abstract BACKGROUND: Keeping in view the serious health and environmental apprehensions associated with the use of pesticides, entomopathogenic nematodes have the potential to supersede larvicidal activity for the management of various insect pests. RESULT: The lab experiments were conducted to test the pathogenicity of two EPNs species S. feltiaeand H. bacteriophora at different (IJs/cm2) concentrations against cabbage pests. Based on the pathogenicity of strains, only two isolates effectively show larvicidal activity. The native isolate was obtained from soil samples, collected from Rajgarh, Hamachi Pradesh, India. Petri dish bioassay use nematodes S. feltiae HR1 and H. bacteriophora HR2 species dose (0, 10, 20, 40, 80, 160/ IJs/cm2). The highest (%) 2nd instar larval mortality was recorded in treatments with H. bacteriophora and S. feltiae @ 160 /IJs/cm-2 were (72.08, 67.42 percent). And 4th instar larval mortality was recorded in treatment with H. bacteriophora, and S. feltiae @ 160 /IJs/cm-2 were (85.38,69.50 percent). The next best treatments in order of their efficacy’s pupae mortality were H. bacteriophora and S. feltiae @ 160 /IJs/cm-2 (74.12, percent) both are seam result, H. bacteriophora and S. feltiae @ 80 /IJs/cm-2 (62.12, 58.58 percent). Larvicidal activity after 48- and 72-hours exposure, the S. feltiae and H. bacteriophora (1.0, 1.30, 1.60, 1.90, 2.20 /IJs/cm2) showed potent larvicidal activity with LC50, LC75 and LC90 of all instars and pupae show high mortality. The strain inhibits the larval and pupal development 48 to 72 hr exposer time with LC50 range from 11.30 to 39.94, LC75 18.15 to 73.54, LC90 61.80 to 99.21.CONCLUSION: These studies demonstrate the challenge for cabbage butterfly P. brassicae. The local indigenous strains of EPNs (S. feltiae HR1, H. bacteriophora HR2) as a good biocontrol agent against, cruciferous vegetables crop pest P. brassicae.

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Laboratory screening for virulent entomopathogenic nematodes (Heterorhabditis bacteriophora and Steinernema yirgalemense) and fungi (Metarhizium anisopliae and Beauveria bassiana) and assessment of possible synergistic effects of combined use against grubs of the barley chafer Coptognathus curtipennis

Nematology ◽

10.1163/156854108785787217 ◽

2008 ◽

Vol 10 (5) ◽

pp. 701-709 ◽

Cited By ~ 17

Author(s):

Samuel A. Anbesse ◽

Bekele J. Adge ◽

Wondirad M. Gebru

Keyword(s):

Metarhizium Anisopliae ◽

Entomopathogenic Nematodes ◽

Insect Pests ◽

Synergistic Effects ◽

Larval Mortality ◽

Integrated Approach ◽

Heterorhabditis Bacteriophora ◽

Insect Larvae ◽

Combined Use ◽

Steinernema Yirgalemense

Abstract The barley chafer grub, Coptognathus curtipennis, is an important field pest of barley, wheat and linseed seedlings at Tikur Inchini district of theWest Shewa zone of Ethiopia in Ormoia regional state. Two entomopathogenic nematodes (EPN; Heterorhabditis bacteriophora and Steinernema yirgalemense, strains ANEPN21, ANEPN22, respectively) and three entomopathogenic fungal isolates of Metarhizium anisopliae (MM, EE and PPRC66) were compared separately at different rates of concentration for their effect against C. curtipennis in laboratory bioassays. Heterorhabditis bacteriophora and M. anisopliae isolate MM were found to be the most pathogenic to C. curtipennis at 1000 infective juveniles (IJ)/grub and 108 conidia (g soil)–1 concentration, causing 88.7% and 88.6% mortality, respectively, after 3-4 weeks exposure. These two most effective entomopathogens were further tested against the same insect in simultaneous and sequential combination with each other. Insect larvae were exposed to optimal concentration of H. bacteriophora and M. anisopliae isolate MM, which caused 50% larval mortality, i.e., 380 IJ/grub and 1.7 × 107 conidia (g soil)–1, respectively, as observed in the first experiments. The combined application increased larval mortality in an additive and synergistic manner. To achieve a strong synergistic effect, larva had to be exposed to M. anisopliae isolate MM 3 weeks before the addition of nematodes. The result of the whole experiment suggests that the damage caused by barley chafer grub can be controlled or managed by applying EPN and/or fungi. Furthermore, the combined use of M. anisopliae with nematodes may offer an integrated approach to increase the efficacy of EPN for C. curtipennis control and perhaps other insect pests.

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SUSCEPTIBILITY OF 31 SPECIES OF AGRICULTURAL INSECT PESTS TO THE ENTOMOGENOUS NEMATODES STEINERNEMA FELTIAE AND HETERORHABDITIS BACTERIOPHORA

The Canadian Entomologist ◽

10.4039/ent117401-4 ◽

1985 ◽

Vol 117 (4) ◽

pp. 401-407 ◽

Cited By ~ 43

Author(s):

O. N. Morris

Keyword(s):

Life Histories ◽

Insect Pests ◽

Biological Control Agent ◽

Larval Mortality ◽

Heterorhabditis Bacteriophora ◽

Control Agent ◽

Delia Radicum ◽

Steinernema Feltiae ◽

Nematode Reproduction ◽

First Time

AbstractThe susceptibility of 31 species of agricultural insect pests representing 7 families of Lepidoptera, 3 of Diptera, and 3 of Coleoptera to the entomogenous nematodes Steinernema feltiae Filipjev and Heterorhabditis bacteriophora Poinar, were investigated in the laboratory. Of the 27 species found susceptible to S. feltiae, 19 are reported for the first time. Of the 21 species killed by H. bacteriophora, 15 are reported for the first time. The Lepidoptera as a group were highly susceptible to the nematodes. On the basis of larval mortality caused by the nematodes, of nematode reproduction in insect cadavers, and of the known economic importance and life histories of the test insects, S. feltiae was judged to warrant further research as a possible biological-control agent against Euxoa ochrogaster (Guenée), E. auxiliaris (Grote), Agrotis orthogonia (Morr.), Actebia fennica (Tauscher), Mamestra configurata Walker, Delia radicum (L.), D. antiqua (Meig.), and Zygogramma exclamationis (F.).

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Virulence comparisons of high-temperature-adapted Heterorhabditis bacteriophora, Steinernema feltiae and S. carpocapsae

Helminthologia ◽

10.1515/helmin-2015-0021 ◽

2015 ◽

Vol 52 (2) ◽

pp. 118-122 ◽

Cited By ~ 1

Author(s):

I. A. Susurluk ◽

T. C. Ulu

Keyword(s):

High Temperature ◽

Entomopathogenic Nematodes ◽

Galleria Mellonella ◽

Insect Pests ◽

Nematode Species ◽

Heterorhabditis Bacteriophora ◽

Steinernema Feltiae ◽

Safe Alternative ◽

Significant Difference ◽

Environmentally Safe

Summary Entomopathogenic nematodes (EPNs) are environmentally safe alternative control agents. Nematodes in the Heterorhabditidae and Steinernematidae families are widely used in biological control frameworks, especially for soil-inhabiting insect pests. In this experiment, Heterorhabditis bacteriophora (Poinar, 1976), Steinernema feltiae (Filipjev, 1934) and S. carpocapsae (Weiser, 1955) adapted at high temperature were assessed in order to detect differences in virulence between adapted and non-adapted populations. All species were exposed to 38 °C for 2 h. After this treatment, live infective juveniles (IJs) were used to infect to last instar Galleria mellonella (Linnaeus, 1758). larvae at the following doses: 1, 2, 3, 4 and 5 IJs/larva. The LD50 and LD90 were obtained for these species. Non-adapted populations of the nematode species were used as controls for this experiment. The results indicated that differences in S. feltiae and S. carpocapsae virulence between the adapted and non-adapted populations were significant; no significant difference was observed between the adapted and non-adapted H. bacteriophora populations.

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Virulence of entomopathogenic nematodes to different stages of the flatheaded root borer, Capnodis tenebrionis (L.) (Coleoptera: Buprestidae)

Nematology ◽

10.1163/156854109x446962 ◽

2009 ◽

Vol 11 (3) ◽

pp. 365-373 ◽

Cited By ~ 10

Author(s):

Ana Morton ◽

Fernando García-del-Pino

Keyword(s):

Biological Control ◽

Entomopathogenic Nematodes ◽

Larval Mortality ◽

Heterorhabditis Bacteriophora ◽

Steinernema Feltiae ◽

Males And Females

AbstractThe virulence of 14 Steinernema feltiae isolates, one S. carpocapsae and three Heterorhabditis bacteriophora isolates was compared in the laboratory against larvae, pupae and adults of the flatheaded root borer, Capnodis tenebrionis. Larval mortality ranged from 50 to 100% and pupae mortality from 0 to 70%. Adults were exposed to different nematode concentrations, 50 and 100 infective juveniles/cm2. Steinernema feltiae caused the highest mortality at the two doses tested, reaching 66.7% and 100%, respectively. Significant differences in mortality between C. tenebrionis males and females were observed. The differences in virulence observed at each stage of C. tenebrionis vary within a species and can be higher than differences among species. The results of this study suggest that some of the isolate strains have potential for improved biological control of C. tenebrionis.

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Effect of mini-sprinkler irrigation system on Heterorhabditis baujardi LPP7 (Nematoda: Heterorhabditidae) infective juvenile

Scientia Agricola ◽

10.1590/s0103-90162008000400017 ◽

2008 ◽

Vol 65 (4) ◽

pp. 433-437 ◽

Cited By ~ 8

Author(s):

Juan Carlos Lara ◽

Cláudia Dolinski ◽

Elias Fernandes de Sousa ◽

Rogério Figueiredo Daher

Keyword(s):

Entomopathogenic Nematodes ◽

Galleria Mellonella ◽

Insect Pests ◽

Irrigation System ◽

Larval Mortality ◽

Sprinkler Irrigation ◽

Infective Juvenile ◽

Application Techniques ◽

Temperature And Pressure ◽

Search Capability

Entomopathogenic nematodes (EPNs) are currently being used as successful biological control agents of soil-dwelling insect pests. Previous field and greenhouse studies demonstrated that application techniques and non-biotic factors (temperature and pressure) have a significant effect on EPNs efficacy. The objective of this study was to evaluate the influence of an irrigation spray application system on the viability, infectivity and host search capability of Heterorhabditis baujardi LPP7 (Nematoda: Heterorhabditidae) infective juveniles (IJ). Two assays were proposed. Their viability was evaluated under the microscope after the IJ passed through the irrigation system. Infectivity on Galleria mellonella larvae, and host search capability, as evidenced by larval mortality, were evaluated in containers (Experiment 1). In the field (Experiment 2), mortality of G. mellonella larvae was evaluated under different nematode concentrations (0, 100,000, 300,000 and 500,000 IJ per tree). No differences were recorded on the viability, infectivity and host search capability of the IJ in Experiment 1. In Experiment 2, differences were recorded among the different concentrations used (p < 0.05), and a higher mortality was observed at the highest nematode concentration (28.3% and 37% in each one of the two experiment repetitions). This irrigation system did not affected adversely the viability, infectivity and host search capability of H. baujardi LPP7.

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UJI EFEKTIVITAS NEMATODA ENTOMOPATOGEN (RHABDITIDA: Steinernema DAN Heterorhabditis) SEBAGAI MUSUH ALAMI NON-ENDEMIK PENGGEREK BATANG PADI KUNING (Scirpophaga incertulas)

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.2771-79 ◽

2007 ◽

Vol 7 (2) ◽

pp. 71-79

Author(s):

Chaerani Chaerani ◽

Bebet Nurbaeti

Keyword(s):

Entomopathogenic Nematodes ◽

Insect Pests ◽

Larval Mortality ◽

Damage Threshold ◽

Stem Borer ◽

Leaf Sheath ◽

Scirpophaga Incertulas ◽

Potted Plants ◽

Plant Damage ◽

Rice Stem Borer

Efficacy Tests of Entomopathogenic Nematodes (Rhabditida: Steinernema dan Heterorhabditis) as Non-endemic Natural Enemies of Yellow Rice stem Borer (Scirpophaga incertulas). Yellow rice stem borer (Scirpophaga incertulas) is a chronic insect pests of irrigated rice and difficult to control. Entomopathogenic nematodes from the genus Steinernema and Heterorhabditis are promising biological control agents for this pest as their infective juveniles (IJs) are capable of seeking and infecting insect living in moist, cryptic habitat such as galleries created by stem borer larvae. Thirteen indigenous and exotic Steinernema and Heterorhabditis sprayed to rice seedlings in laboratory with nematodes at concentrations of 0.5 or 2.0×104 IJs ml-1 water caused larval mortality between 7–93%. Further test in greenhouse on nematodes that had >50% efficacy showed that an indigenous isolate, H. indicus INA H17, was the most effective among the tested nematodes in killing larvae or pupae (78%). Reduction in plant damage caused by the insects could not be demonstrated as the trials was limited to potted plants. The survival ability of nematodes on rice plants was evaluated by using INA H4 as an example. A low percentage of INA H4 IJs (0.5%) persisted in inner leaf sheath until 7 days post application, while IJs on leaf surface and outer leaf sheath survived only until 2 and 48 hours post application, respectively. Improvement of application strategies including repeated spray, addition of antidessicant and adjusment of spray volume and application at damage threshold or plant critical period are deemed necessary to enhance nematode efficacy and reduce plant damage in the field.

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Production and Infectivity of Some Entomopathogenic Nematodes Against Larvae and Pupae of Cabbage Butterfly, Pieris brassicae L. (Lepidoptera:Pieridae)

Journal of Entomology ◽

10.3923/je.2005.86.91 ◽

2004 ◽

Vol 2 (1) ◽

pp. 86-91 ◽

Cited By ~ 1

Author(s):

A.N. Mahar ◽

N.D. JAN ◽

Q.I. Chachar ◽

G.S. Markhand ◽

M. Munir ◽

...

Keyword(s):

Entomopathogenic Nematodes ◽

Pieris Brassicae ◽

Cabbage Butterfly

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Dissemination of Isaria fumosorosea Spores by Steinernema feltiae and Heterorhabditis bacteriophora

Journal of Fungi ◽

10.3390/jof6040359 ◽

2020 ◽

Vol 6 (4) ◽

pp. 359

Author(s):

Jiří Nermuť ◽

Jana Konopická ◽

Rostislav Zemek ◽

Michal Kopačka ◽

Andrea Bohatá ◽

...

Keyword(s):

Entomopathogenic Nematodes ◽

Heterorhabditis Bacteriophora ◽

Potato Dextrose Agar ◽

Steinernema Feltiae ◽

Soil Organisms ◽

Isaria Fumosorosea ◽

Second Stage ◽

Glass Tubes ◽

First Time ◽

Globally Distributed

Entomopathogenic nematodes and fungi are globally distributed soil organisms that are frequently used as bioagents in biological control and integrated pest management. Many studies have demonstrated that the combination of biocontrol agents can increase their efficacy against target hosts. In our study, we focused on another potential benefit of the synergy of two species of nematodes, Steinernema feltiae and Heterorhabditis bacteriophora, and the fungus Isaria fumosorosea. According to our hypothesis, these nematodes may be able to disseminate this fungus into the environment. To test this hypothesis, we studied fungal dispersal by the nematodes in different arenas, including potato dextrose agar (PDA) plates, sand heaps, sand barriers, and glass tubes filled with soil. The results of our study showed, for the first time, that the spreading of both conidia and blastospores of I. fumosorosea is significantly enhanced by the presence of entomopathogenic nematodes, but the efficacy of dissemination is negatively influenced by the heterogeneity of the testing arena. We also found that H. bacteriophora spread fungi more effectively than S. feltiae. This phenomenon could be explained by the differences in the presence and persistence of second-stage cuticles or by different foraging behavior. Finally, we observed that blastospores are disseminated more effectively than conidia, which might be due to the different adherence of these spores (conidia are hydrophobic, while blastospores are hydrophilic). The obtained results showed that entomopathogenic nematodes (EPNs) can enhance the efficiency of fungal dispersal.

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Potential of Moroccan entomopathogenic nematodes for the control of the Mediterranean fruit fly Ceratitis capitata Wiedemann (Diptera: Tephritidae)

Scientific Reports ◽

10.1038/s41598-020-76170-7 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Fouad Mokrini ◽

Salah-Eddine Laasli ◽

Youssef Benseddik ◽

Abdelmalek Boutaleb Joutei ◽

Abdelali Blenzar ◽

...

Keyword(s):

Mortality Rate ◽

Entomopathogenic Nematodes ◽

Ceratitis Capitata ◽

Larval Mortality ◽

Fruit Fly ◽

Fruit Production ◽

Prunus Armeniaca ◽

Mediterranean Fruit Fly ◽

Steinernema Feltiae ◽

The Mediterranean

Abstract The Mediterranean fruit fly, Ceratitis capitata Wiedemann, is a deleterious pest worldwide affecting fruit production. The entomopathogenic nematodes (EPNs) are a potential biocontrol agent that could be effectively used to control this Mediterranean fruit fly. In this study, five EPN strains reported from different fields in Morocco were evaluated for their efficacy against C. capitata. In laboratory assays, Steinernema feltiae-SF-MOR9, S. feltiae-SF-MOR10 and Heterorhabditis bacteriophora-HB-MOR7 strains showed significantly higher infectivity and penetration rates when compared to the other strains. S. feltiae-SF-MOR9 caused the highest larval mortality rate (80%) at 50 infective juveniles (IJs) cm−2. However, additional results showed that both S. feltiae strains were significantly effective in controlling C. capitata larvae in apricot (Prunus armeniaca) fruits on soil surface with high mortality rate at 50 and 100 IJs cm−2. Different soil textures and moisture levels resulted in a significant variation in EPN strain virulence against C. capitata. Sandy clay loam soil in combination with 50 IJs cm−2 of S. feltiae (SF-MOR9 or SF-MOR10) caused a higher mortality rate of C. capitata larvae. Furthermore, applying these EPN strains at 50–100 IJs cm−2 in combination with 10–15% moisture level showed optimal results against C. capitata larvae. Therefore, those two Moroccan EPN strains could be used as promising eco-friendly biological agents against C. capitata.

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