scholarly journals Effectiveness of fungal, bacterial and yeast antagonists for management of mango anthracnose (Colletotrichum gloeosporioides)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Adikshita Sharma ◽  
Inder Mohan Sharma ◽  
Monica Sharma ◽  
Kishor Sharma ◽  
Amit Sharma
Keyword(s):  
Disease Management ◽  
Disease Control ◽  
Colletotrichum Gloeosporioides ◽  
Agar Medium ◽  
Field Conditions ◽  
Pichia Anomala ◽  
Malt Extract ◽  
Potato Dextrose Agar Medium ◽  
Anthracnose Disease ◽  
Post Harvest

Abstract Background Mango anthracnose, caused by Colletotrichum gloeosporioides, is one of the most important diseases of mango crop. It mainly attacks leaves, flowers, young fruits and twigs and also appears as a post-harvest disease of ripened fruits. Application of bio-control agents has huge potential in plant disease management. The goal of the present research was to establish the potential of individual and combined bio-control agents for the management of mango anthracnose under in vitro and under field conditions. Results The antagonistic reaction of six fungi, six bacteria and nine yeasts against C. gloeosporioides on potato dextrose agar medium and malt extract agar medium was observed among which Trichoderma harzianum was found to be the most efficient with 89.26% mycelial growth inhibition. Evaluation of bio-control agents against anthracnose disease development on mango fruit revealed that dip treatment of mango fruits in spore suspension (1.2 × 104 cfu/ml) of T. harzianum for 5 min was the most effective and provided disease control to the tune of 81.67%. Combined application of effective bio-control agents as a post-harvest fruit dip treatment was also evaluated against the mango anthracnose on mango fruits, where the treatment of T. harzianum + Pichia anomala was very effective with 93.39% disease control. Under field conditions, three consecutive sprays of T. harzianum, starting with the initiation of disease on leaves, followed by other two sprays at an interval of 15 days during 2015 and 2016 were found the best for the management of mango anthracnose disease both on leaves and on fruits at two locations. Conclusions The combined and individual applications of bio-control agents, viz.T. harzianum, Bacillus subtilis and P. anomala, through foliar spray or by fruit dip had the potential to control mango anthracnose. The bio-formulations of these bio-control agents had the potential to replace chemical fungicides and also protect the natural environment, thus playing a significant role in integrated disease management.

A comparison of the efficacy of six media for isolating wood-decaying Hymenomycetes

1995 ◽  
Vol 41 (1) ◽  
pp. 104-107 ◽  
Author(s):  
Gary C. Johnson
Keyword(s):  
Agar Medium ◽  
Wood Decay ◽  
Brown Rot ◽  
Potato Dextrose Agar ◽  
Malt Extract ◽  
Decay Fungi ◽  
Potato Dextrose Agar Medium ◽  
Wood Decay Fungi ◽  
Isolation Media ◽  
Decaying Wood

Six media reported to be useful for isolating Hymenomycetes from decaying wood were compared. Plates containing these media were inoculated with small segments taken from decaying wood collected in the field. Hymenomycetes and other microorganisms that grew from the segments were recorded. A malt extract – potato dextrose agar medium that incorporated benomyl, neomycin, and streptomycin was the most successful at isolating the white or brown rot fungi.Key words: isolation media, Hymenomycetes, Basidiomycotina, wood decay fungi, benomyl.

scholarly journals Fungicidal management of Alternaria alternata (Fr.) Keissler causing blight of gerbera (Gerbera jamesonii H. Bolus ex J.D. Hook)

2012 ◽  
Vol 4 (2) ◽  
pp. 220-227 ◽  
Author(s):  
Dipak T. Nagrale ◽  
Anil P. Gaikwad ◽  
Sanjay Goswami ◽  
Lalan Sharma
Keyword(s):  
Disease Management ◽  
Disease Control ◽  
Alternaria Alternata ◽  
Fungal Pathogen ◽  
Management Practices ◽  
Bordeaux Mixture ◽  
Gerbera Jamesonii ◽  
Leaf Spots ◽  
Aerial Parts ◽  
Blight Disease

Alternaria, the fungal pathogen has wide host range generally attacks the aerial parts of plants causing leaf spots and blights. Gerbera is a genus of ornamental flower plants. Gerbera plants are infected by many diseases. Different disease management practices are adopted in gerbera cultivation. The fungicidal management of Alternaria blight is one of the important strategies for the disease management in gerbera in polyhouse condition. In this study, preventive and curative fungicidal sprays were adopted for the management of blight disease in polyhouse. This study revealed that preventive fungicidal sprays were significant over curative fungicidal sprays for the management of Alternaria alternata blight of gerbera (Gerbera jamesonii H. Bolus ex J.D. Hook) in polyhouse. The preventive sprays made of Bordeaux mixture (0.6 %), tricyclazole (0.1%) and iprodione + carbendazim (0.1%) fungicides were found effective with 95.85 %, 96.59 % and 95.88 % disease control respectively, under polyhouse condition.

scholarly journals Differentiation of Colletotrichum gloeosporioides isolates by using total proteins and esterase electrophoretic patterns and extracellular enzymes production

2010 ◽  
Vol 36 (2) ◽  
pp. 140-144 ◽  
Author(s):  
Tereza Cristina de Assis ◽  
Maria Menezes ◽  
Domingos Eduardo Guimarães Tavares de Andrade ◽  
Rildo Sartori Barbosa Coelho
Keyword(s):  
Extracellular Enzymes ◽  
Colletotrichum Gloeosporioides ◽  
Electrophoretic Analysis ◽  
Solid Substrate ◽  
Causal Agent ◽  
Total Proteins ◽  
Anthracnose Disease ◽  
Electrophoretic Patterns

Isolates of Colletotrichum gloeosporioides (ISO-1, ISO-2, ISO-3, ISO-4, ISO-5 and ISO-6), the causal agent of anthracnose disease on mango fruits, were characterized by electrophoretic patterns of total proteins and esterase in polyacrylamida gel, and also, by production of extracellular enzymes on specific solid substrate. The electrophoretic analysis showed variation in number, intensity of coloration and position of the bands in the gel at each studied system tested. In contrast to the monomorphic behavior to total proteins, high esterase polymorfism was observed indicating difference among isolates. All isolates showed the activity of extracellular enzymes such as amylase, lipase, and protease with some variation among them. The proteolitic activity seemed to be more accentuated than the two other enzymes studied.

scholarly journals Airborne Aspergillus at some rural areas adjoining to Raipur city (C.G.) India

2020 ◽  
Vol 26 (2) ◽  
Author(s):  
Ritu Kunjam ◽  
V.K. Kanungo ◽  
S.K. Jadhav
Keyword(s):  
Aspergillus Niger ◽  
Rural Areas ◽  
Meteorological Parameters ◽  
Agar Medium ◽  
Fungal Species ◽  
Aspergillus Clavatus ◽  
Potato Dextrose Agar Medium ◽  
Percentage Contribution ◽  
Different Seasons

Increased urbanization and industrialization in recent time has made a significant impact on air quality of the area. The atmosphere is rich in propagule of different fungal species. The investigation on airborne Aspergillus contribution was conducted in Periphery of Raipur city from February, 2018 to March, 2019 with the help of gravity petriplate containing PDA (Potato Dextrose Agar) medium. In this study, total 11 species of Aspergillus were recorded. The percentage frequency and percentage contribution of different Aspergillus species were different in different seasons. Aspergillus niger was most frequent throughout the year followed by Aspergillus fumigatus, A. flavus, and A. nidulans etc. While Aspergillus clavatus, and A. versicolor, A. aculeatus were the least frequent species. The result indicated the highest percentage contribution of Aspergillus niger (43.29 percent) followed by A. fumigatus (9.02percent), A. flavus (8.42 percent) while A. clavatus (0.21 percent). The objective of the studies was to determine a seasonal variation in concentrations of Aspergillus on the basis of meteorological parameters.

scholarly journals Host-induced silencing of the Colletotrichum gloeosporioides conidial morphology 1 gene (CgCOM1) confers resistance against Anthracnose disease in chilli and tomato

2020 ◽  
Vol 104 (4-5) ◽  
pp. 381-395
Author(s):  
Binod Kumar Mahto ◽  
Anjulata Singh ◽  
Manish Pareek ◽  
Manchikatla V. Rajam ◽  
Swatismita Dhar-Ray ◽  
...  
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Anthracnose Disease ◽  
Conidial Morphology
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