scholarly journals Comparative expression analysis of Phytophthora sojae polysaccharide lyase family 3 (pectate lyase) genes during infection of the soybean Glycine max

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Nicholas Grams ◽  
Hannah Komar ◽  
Dylan Jainchill ◽  
Manuel Ospina-Giraldo
Keyword(s):  
Glycine Max ◽  
Expression Analysis ◽  
Pectate Lyase ◽  
Phytophthora Sojae ◽  
Polysaccharide Lyase ◽  
Comparative Expression Analysis

scholarly journals Screening of a Novel Polysaccharide Lyase Family 10 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression and Characterization

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2774 ◽  
Author(s):  
Yan Zhao ◽  
Ye Yuan ◽  
Xinyu Zhang ◽  
Yumei Li ◽  
Qiang Li ◽  
...  
Keyword(s):  
Textile Industry ◽  
Pectate Lyase ◽  
Paenibacillus Polymyxa ◽  
Polygalacturonic Acid ◽  
Optimal Conditions ◽  
Citrus Pectin ◽  
Plant Fiber ◽  
Polysaccharide Lyase ◽  
Glycosidic Bonds ◽  
Fiber Processing

Pectate lyase (EC 4.2.2.2) catalyzes the cleavage of α-1,4-glycosidic bonds of pectin polymers, and it has potential uses in the textile industry. In this study, a novel pectate lyase belonging to polysaccharide lyase family 10 was screened from the secreted enzyme extract of Paenibacillus polymyxa KF-1 and identified by liquid chromatography-MS/MS. The gene was cloned from P. polymyxa KF-1 genomic DNA and expressed in Escherichia coli. The recombinant enzyme PpPel10a had a predicted Mr of 45.2 kDa and pI of 9.41. Using polygalacturonic acid (PGA) as substrate, the optimal conditions for PpPel10a reaction were determined to be 50 °C and pH 9.0, respectively. The Km, vmax and kcat values of PpPel10a with PGA as substrate were 0.12 g/L, 289 μmol/min/mg, and 202.3 s−1, respectively. Recombinant PpPel10a degraded citrus pectin, producing unsaturated mono- and oligogalacturonic acids. PpPel10a reduced the viscosity of PGA, and weight loss of ramie (Boehmeria nivea) fibers was observed after treatment with the enzyme alone (22.5%) or the enzyme in combination with alkali (26.3%). This enzyme has potential for use in plant fiber processing.

Comparative expression analysis of five caulimovirus promoters in citrus

2016 ◽  
Vol 126 (2) ◽  
pp. 229-238 ◽  
Author(s):  
M. Dutt ◽  
L. Erpen ◽  
G. Ananthakrishnan ◽  
G. A. Barthe ◽  
R. H. Brlansky ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Comparative Expression Analysis

Common Phytophthora sojae pathotypes occurring in South Dakota

2021 ◽  
Author(s):  
Rawnaq Chowdhury ◽  
Connie Tande ◽  
Emmanuel Z Byamukama
Keyword(s):  
Glycine Max ◽  
South Dakota ◽  
Resistance Genes ◽  
Phytophthora Sojae ◽  
Stem Rot ◽  
Inoculation Technique ◽  
Glycine Max L ◽  
Rps Gene ◽  
Important Disease

Phytophthora root and stem rot, caused by Phytophthora sojae, is an important disease of soybean (Glycine max L.) in South Dakota. Because P. sojae populations are highly diverse and resistance genes deployed in commercial soybean varieties often fail to manage the disease, this study was initiated to determine P. sojae pathotype distribution in South Dakota. A total of 216 P. sojae isolates were baited from soil collected from 422 soybean fields in South Dakota in 2013-2015 and 2017. The pathotype of each isolate was determined by inoculating 10 seedlings of 13 standard soybean P. sojae differential lines using the hypocotyl inoculation technique. Of the 216 pathotyped isolates, 48 unique pathotypes were identified. The virulence complexity of isolates ranged from virulence on one Rps gene (Rps7) to virulence on 13 Rps genes and mean complexity was 5.2. Harosoy (Rps7), Harlon (Rps1a), Williams 79 (Rps 1c), William 82 (Rps1k), Harosoy 13XX (Rps1b), were susceptible to 98, 80, 78, 73, 72% of the isolates, respectively. These results highlight the highly diverse P. sojae pathotypes in South Dakota and the likely Rps genes to fail in commercial soybean varieties

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