scholarly journals Phylogenetic analysis of phytochrome A gene from Lablab purpureus (L.) Sweet

Author(s):  
Stuti Krishna ◽  
Kaushal Modha ◽  
Vipulkumar Parekh ◽  
Ritesh Patel ◽  
Digvijay Chauhan

Abstract Background Phytochromes are the best characterized photoreceptors that perceive Red (R)/Far-Red (FR) signals and mediate key developmental responses in plants. It is well established that photoperiodic control of flowering is regulated by PHY A (phytochrome A) gene. So far, the members of PHY A gene family remains unexplored in Lablab purpureus, and therefore, their functions are still not deciphered. PHYA3 is the homologue of phytochrome A and known to be involved in dominant suppression of flowering under long day conditions by downregulating florigens in Glycine max. The present study is the first effort to identify and characterize any photoreceptor gene (PHYA3, in this study) in Lablab purpureus and decipher its phylogeny with related legumes. Results PHYA3 was amplified in Lablab purpureus cv GNIB-21 (photo-insensitive and determinate) by utilizing primers designed from GmPHYA3 locus of Glycine max. This study was successful in partially characterizing PHYA3 in Lablab purpureus (LprPHYA3) which is 2 kb longer and belongs to exon 1 region of PHYA3 gene. Phylogenetic analysis of the nucleotide and protein sequences of PHYA genes through MEGA X delineated the conservation and evolution of Lablab purpureus PHYA3 (LprPHYA3) probably from PHYA genes of Vigna unguiculata, Glycine max and Vigna angularis. A conserved basic helix-loop-helix motif bHLH69 was predicted having DNA binding property. Domain analysis of GmPHYA protein and predicted partial protein sequence corresponding to exon-1 of LprPHYA3 revealed the presence of conserved domains (GAF and PAS domains) in Lablab purpureus similar to Glycine max. Conclusion Partial characterization of LprPHYA3 would facilitate the identification of complete gene in Lablab purpureus utilizing sequence information from phylogenetically related species of Fabaceae. This would allow screening of allelic variants for LprPHYA3 locus and their role in photoperiod responsive flowering. The present study could aid in modulating photoperiod responsive flowering in Lablab purpureus and other related legumes in near future through genome editing.

2020 ◽  
Vol 17 (5) ◽  
pp. 640-654
Author(s):  
Hamidreza Akrami ◽  
Bibi Fatemeh Mirjalili ◽  
Omidreza Firuzi ◽  
Azadeh Hekmat ◽  
Ali Akbar Saboury ◽  
...  

Background: Chromene and anilinopyrimidine heterocyclics are attractive anticancer compounds that have inspired many researchers to design novel derivatives bearing improved anticancer activity. Methods: A series of pyrimidine-fused benzo[f]chromene derivatives 6a-x were synthesized as anticancer hybrids of 1H-benzo[f]chromenes and anilinopyrimidines. The inhibitory activity of the synthesized compounds 6a-x against cell viability of human chronic myelogenous leukemia (K562), human acute lymphoblastic leukemia (MOLT-4) and human breast adenocarcinoma (MCF-7) cell lines was evaluated using MTT assay. The interaction of the most promising compound with calf-thymus DNA was also studied using spectrometric titrations and Circular Dichroism (CD) spectroscopy. Results: Most compounds showed promising activity against tested cell lines. Among them, 2,4- dimethoxyanilino derivative 6g exhibited the best profile of activity against tested cell lines (IC50s = 1.6-6.1 μM) with no toxicity against NIH3T3 normal cell (IC50 >200 μM). The spectrometric studies exhibited that compound 6g binds to DNA strongly and may change DNA conformation significantly, presumably via a groove binding mechanism. Conclusion: The results of this study suggest that the prototype compound 6g can be considered as a novel lead compound for the design and discovery of novel anticancer agents.


2008 ◽  
pp. 3054 ◽  
Author(s):  
Qin Jiang ◽  
Zhengyi Wu ◽  
Yangmiao Zhang ◽  
Anna C. G. Hotze ◽  
Michael J. Hannon ◽  
...  

2020 ◽  
Vol 2 (1) ◽  
pp. 23

Melanin is nearly a ubiquitous pigment synthesized by living organisms in the course of hydroxylation and polymerization. Melanin has immense application potential in the field of agriculture, cosmetics, and pharmaceutical industries. The aim of this study was to obtain the melanin pigment produced by Bacillus subtilis using T medium and study the biological and chemical characteristics of the pigment. Melanin pigment production in Bacillus was analyzed and was optimized at different temperatures and pH for optimal production. The pigment was confirmed by its chemical characterization. The melanin pigment obtained was water-soluble and was confirmed to be photoprotective using Ultraviolet-Visible spectrum analysis, which showed maximum absorption in the UV region (200-300 nm), but diminished towards the visible regions. The pigment also showed antioxidant activity. Fourier Transformation Infrared spectroscopy analysis confirmed the crude melanin extract obtained as melanin. DNA binding property of melanin was studied. UV- Visible spectroscopic methods shows that melanin is able to bind DNA and impact protection. The pigment was analyzed for its application in the field of agriculture. It had shown to impart UV protection to UV exposed seeds during germination. Melanin also found to enhance the growth of plants when studied under laboratory conditions.


2019 ◽  
Vol 90 ◽  
pp. 103074
Author(s):  
Gang Li ◽  
Haodong Tang ◽  
Chuanfeng Liu ◽  
Xiaoyu Liao ◽  
Sicong Li ◽  
...  

2006 ◽  
Vol 81 (4) ◽  
pp. 1990-2001 ◽  
Author(s):  
Noriaki Yamamoto ◽  
Masato Suzuki ◽  
Masa-aki Kawano ◽  
Takamasa Inoue ◽  
Ryou-u Takahashi ◽  
...  

ABSTRACT Adeno-associated virus (AAV) integrates site specifically into the AAVS1 locus on human chromosome 19. Although recruitment of the AAV nonstructural protein Rep78/68 to the Rep binding site (RBS) on AAVS1 is thought to be an essential step, the mechanism of the site-specific integration, particularly, how the site of integration is determined, remains largely unknown. Here we describe the identification and characterization of a new cellular regulator of AAV site-specific integration. TAR RNA loop binding protein 185 (TRP-185), previously reported to associate with human immunodeficiency virus type 1 TAR RNA, binds to AAVS1 DNA. Our data suggest that TRP-185 suppresses AAV integration at the AAVS1 RBS and enhances AAV integration into a region downstream of the RBS. TRP-185 bound to Rep68 directly, changing the Rep68 DNA binding property and stimulating Rep68 helicase activity. We present a model in which TRP-185 changes the specificity of the AAV integration site from the RBS to a downstream region by acting as a molecular chaperone that promotes Rep68 complex formation competent for 3′→5′ DNA helicase activity.


2014 ◽  
Vol 24 (6) ◽  
pp. 2314-2324 ◽  
Author(s):  
Mo Zhu ◽  
Likai Zhou ◽  
Yuchao Yao ◽  
Shuai Li ◽  
Mengjiao Lv ◽  
...  

2016 ◽  
Vol 27 (11) ◽  
pp. 1708-1716 ◽  
Author(s):  
Li Zhang ◽  
Yu-Chao Yao ◽  
Meng-Ying Gao ◽  
Rui-Xue Rong ◽  
Ke-Rang Wang ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document