scholarly journals Effects of plant growth regulators and growing media on propagation and field establishment of Stevia rebaudiana: a medicinal plant of commerce

2022 ◽  
Vol 3 (1) ◽  
Author(s):  
K. Abha Manohar ◽  
Gopal Shukla ◽  
Bidhan Roy ◽  
Sumit Chakravarty
Keyword(s):  
Growth Hormone ◽  
Plant Growth ◽  
Medicinal Plant ◽  
Plant Growth Regulators ◽  
Exposure Time ◽  
Growth Regulators ◽  
West Bengal ◽  
Stevia Rebaudiana ◽  
Hormone Treatment ◽  
Growing Media

Abstract Background Stevia rebaudiana is an economically important medicinal plant that has generated interest among the growers and pharmacologists in terms of its industrial or pharmaceutical value. For the mass production of the seedlings, easy and convenient techniques are lacking while, micro propagation was reported promising but still out of reach at farm level. The unavailability of quality planting materials due to non-viable seeds is restricting its mass commercial scale cultivation. The present study was therefore attempted to standardize the plant growth regulators and growing media to standardize the vegetative propagation protocol through cuttings for its mass multiplication in Terai region of West Bengal, India. Methods Growing media (soil, FYM, saw dust and sand) as sole and in combination and growth hormones (IAA, IBA and NAA in different concentration and a commercial formulation i.e. Totoroot© with different exposure time) were compared with control (i.e. sole soil and no hormone treatment, respectively) to standardize the nursery protocol of Stevia. Results Sand used as sole was found the best growing media for survival and growth of cuttings while, cuttings treated with commercial growth hormone formulation for 5 mins was best. Cuttings treated with commercial growth hormone formulation performed significantly better in the field with respect to survival, growth and production of leaves. Conclusion The study recommends the use of sole sand media and commercial growth hormone formulation with 5 mins exposure time for mass nursery production of Stevia cuttings in Terai zone of West Bengal due to their better performance both in the nursery and after transplanting in the field.

scholarly journals In-vitro Callogenesis and Screening of Antimicrobial Activity of Callus and Seed of Caesalpinia bonducella F.: A Threatened Medicinal Plant of Western Ghats

2021 ◽  
pp. 76-89
Author(s):  
Rajani Shirsat ◽  
Ajit Kengar ◽  
Aruna Rai
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Plant Growth ◽  
Medicinal Plant ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Western Ghats ◽  
Callus Formation ◽  
Caesalpinia Bonducella

Aim: Caesalpinia bonducella Flem is a dioecious scrambling woody liana of Caesalpinoideae, a subfamily of Leguminosae. The plant is threatened and distributed in the deciduous forests of the Western Ghats of India. Being an important medicinal plant Caesalpinia bonducella F. attracted many scientists to exploit various activities associated with a number of phytoconstituents. The present study was undertaken to evaluate the most suitable media and suitable concentrations of plant growth regulators for in vitro Callogenesis and screening of antimicrobial activity of callus and seed of Caesalpinia bonducella. Materials and Methods: Callus was initiated from stem explants, on 1x and 0.5x MS medium plus supplements. The effects of plant growth regulators on callus cultures were studied and observations were made. The in vitro antibacterial activity was performed by using extracts of callus and seed of Caesalpinia bonducella in petroleum ether and methanol against multidrug resistance organisms. The organic extracts of seed and callus of the plant at concentrations of 0.02 mg/ml - 0.1 mg/ml were taken and their activities were measured.   Results: The combination of 2.5 mgL-1 2,4-D  with 2 mgL-1 BAP, resulted in the highest frequency and the highest mean percentage of callus formation (2.35 ± 0.294) with yellow friable callus. The results revealed that all the extracts had a variable degree of antibacterial activity. Conclusions: It was observed that 2,4-D at 2.5 mgL-1 in combination with BAP,2.0 mgL-1 BAP resulted in early initiation, highest induction percentage, with frequency highest mean percentage of callus formation, Antimicrobial tests with methanol and diethyl ether extract of Caesalpinia bonducella seed powder against the clinical isolates showed the zone of inhibition for all the pathogens tested with concentration of methanolic extract of C. bonducella seed powder.

scholarly journals THE ROLL OF SOME PLANT GROWTH REGULATORS ON SHOOTS MULTIPLICATION OF STEVIA PLANTS IN VITRO

2017 ◽  
Vol 48 (5) ◽  
Author(s):  
Al-Obaidy & Khierallah
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Culture Media ◽  
Stevia Rebaudiana ◽  
Shoot Multiplication ◽  
Dry Weight ◽  
Fresh Weight ◽  
Number Of Shoots

This research was conducted to study the effect of some plant growth regulators on in vitro shoots multiplication of stevia (Stevia rebaudiana Bertoni). The experiments included tests of various combinations of KIN with IBA or IAA in the shoot multiplication. Results indicated that KIN at 1.0 mg. L-1 plus 0.3 mg. L-1 of IBA produced the highest number of shoots (3.5 shoots) while KIN at 1.5 mg. L-1 plus IBA at 1.0 mg. L-1 produced the lowest shoot length (1.14 cm).  Hormone free medium produced the highest rate of the leaves number reached 28.56 leaves. KIN and IBA interaction increased fresh and dry weight significantly.   Treatment contained 2.0 mg -1 KIN plus 0.3 mg. L-1 IBA produced the highest fresh weight (1.739 g) while 0.5 mg. L-1 KIN and 0.3 mg. L-1 IBA produced the highest dry weight (0.822 g). As for the effect of interaction between the IAA and KIN it was significant in the number of shoots formed. Interaction between 1.0 mg. L-1 KIN with 0.1 mg. L-1IAA produced the highest number of shoots (3.8 shoots). Shoots length reached 8.10 cm in the media with 0.3 mg. L-1 IAA only. The highest fresh weight (1.267 g) was achieved with the interaction between 1.0 mg. L-1 KIN and 0.3 mg. L-1 IAA while 0.5 mg. L-1IAA without KIN produced the highest dry weight reached 0.138 g.  Shoots multiplication was improved by incorporation of the cytokinin TDZ in culture media. Shoots number, fresh and dry weights were increased significantly by adding 0.05 mg. L-1 of TDZ at present of 0.3 mg. L-1 of IBA giving 6.6 shoots, 0.974 g and 0.144 g respectively while shoots length decreased significantly as media without TDZ produced the highest shoots length reached 9.32 cm. The above results can adopt for the successful in vitro shoot multiplication of Stevia plants. 

Plant growth regulators affecting in vitro cultivation of Stevia rebaudiana

Sugar Tech ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 254-259 ◽  
Author(s):  
Ibrahim A. Ibrahim ◽  
Mahmoud I. Nasr ◽  
Berlanti R. Mohammed ◽  
Mohammed M. El-Zefzafi
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Stevia Rebaudiana ◽  
In Vitro Cultivation

scholarly journals Effect of Growing Media and Plant Growth Regulators on Rooting of Different Types of Stem Cuttings in Acid-Lime Cv. Kagzi

2019 ◽  
Vol 8 (10) ◽  
pp. 2589-2605
Author(s):  
Ashis Malakar ◽  
D. P. Prakasha ◽  
H. Kulapati ◽  
Sanjeevraddi G. Reddi ◽  
S. G. Gollagi ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Stem Cuttings ◽  
Growing Media ◽  
Different Types ◽  
Acid Lime

scholarly journals Micropropagation of Stevia rebaudiana plants

2020 ◽  
Vol 50 (1) ◽  
Author(s):  
Marta Teresa Rokosa ◽  
Danuta Kulpa
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Stevia Rebaudiana ◽  
In Vitro Cultures ◽  
Optimum Composition ◽  
Ms Medium ◽  
Single Node ◽  
Stevia Rebaudiana Bertoni

ABSTRACT: The aim of the study was to develop optimum composition of plant growth regulators in media for the propagation and rooting of shoots of stevia (Stevia rebaudiana Bertoni) in in vitro cultures. Single-node shoot fragments obtained from plants propagated on MS medium were placed onto media supplemented with: BAP, 2iP and KIN at concentrations: 0.5, 1, 2 and 5 mg∙dm-3, whereas at the rooting stage with addition of: IAA, IBA and NAA at concentrations 1, 2, 4 and 8 mg∙dm-3. The highest number of shoots and leaves was reported for plants propagated on MS medium enriched with 0.5 mg∙dm-3 BAP. The greatest number of the longest roots was developed by stevia on the MS medium enriched with 1 mg∙dm-3 IAA.

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