scholarly journals Microdetermination of Adrenocortical Steroids by Double Isotope Method. I. Determination of Cortisol and Corticosterone in Blood as Thiosemicarbazone-35S

1973 ◽  
Vol 21 (3) ◽  
pp. 503-510
Author(s):  
AKIRA KUNUGI ◽  
USHIHO MATSUMOTO ◽  
YOSHIO AIZAWA ◽  
YUZO NAGASE
Keyword(s):  
Isotope Method ◽  
Double Isotope

SIMULTANEOUS DETERMINATION OF THE CONTENT OF SEROTONIN, DOPAMINE, NORADRENALINE AND ADRENALINE IN PANCREATIC ISLETS ISOLATED FROM FED AND STARVED MICE

1977 ◽  
Vol 86 (4) ◽  
pp. 820-832 ◽  
Author(s):  
S. E. Hansen ◽  
C. J. Hedeskov
Keyword(s):  
Pancreatic Islets ◽  
Biogenic Amines ◽  
Simultaneous Determination ◽  
Isotope Method ◽  
Highly Sensitive ◽  
Wet Weight ◽  
Acinar Tissue ◽  
Noradrenaline And Adrenaline ◽  
Double Isotope

ABSTRACT A highly sensitive double isotope method for the simultaneous determination of serotonin, dopamine, noradrenaline and adrenaline has been developed. Advantages and limitations of the method are discussed. The mentioned biogenic amines are all present in isolated pancreatic islet tissue from albino mice in concentrations ranging from approximately 5-30 μmol per kg wet weight (0.8–5 × 10−3 pmol/ng DNA). A somewhat higher content of these amines, especially dopamine, was found in pancreatic acinar tissue. The hypothesis that the impaired glucose-induced insulin secretion during starvation partly is caused by an increased content of biogenic amines in the pancreatic islets was not supported by our experiments which showed an unchanged islet content of these amines after 48 h starvation.

A Rapid and Practical Determination of Aldosterone in Urine by Double Isotope Method

1971 ◽  
Vol 18 (6) ◽  
pp. 517-525
Author(s):  
KIKUO ARAKAWA ◽  
USHIO GOTO ◽  
MOTOOMI NAKAMURA
Keyword(s):  
Isotope Method ◽  
Double Isotope

The use of a double isotope method in the determination of antibody affinity

1973 ◽  
Vol 3 (4) ◽  
pp. 357-363 ◽  
Author(s):  
Susan Gaze ◽  
N.J. West ◽  
M.W. Steward
Keyword(s):  
Antibody Affinity ◽  
Isotope Method ◽  
Double Isotope

EFFECT OF HEPARIN ON THE CORTICOSTERONE SECRETION RATE WITH A DESCRIPTION OF THE DOUBLE ISOTOPE METHOD USED

1967 ◽  
Vol 56 (1_Suppl) ◽  
pp. S140 ◽  
Author(s):  
A. F. Casparie ◽  
Th. J. Benraad ◽  
P. W. C. Kloppenborg ◽  
C. L. H. Majoor
Keyword(s):  
Secretion Rate ◽  
Isotope Method ◽  
Corticosterone Secretion ◽  
Double Isotope
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