Reproductive characteristics of the giant gurami sago strain (Osphronemus goramy Lacepède, 1801): basic knowledge for a future hatchery development strategy

Background: The giant gourami sago strain (Osphronemus goramy Lacepède) has been approved in 2018 as a candidate for freshwater aquaculture in Indonesia. However, information on the species’ reproduction is minimal. This study analyzed the reproductive characteristics of the gourami sago strain broodfish to provide basic knowledge for a future hatchery development strategy. Methods: A total of 10 female and 10 male mature gourami sago strain broodfish were measured for body weight and length, and were evaluated for their reproductive characteristics. Breeding fish were spawned naturally in a 2×1×0.6 m concrete pond with a male-female sex ratio of 1:1. Egg weight and diameter were measured in 25 eggs per female using, respectively, ACIS AD- 600i scales with 0.01 g accuracy, and a microscope (Labo model L-711) using Canon Digital Camera Software 3 . Semen was collected using plastic syringes in 3 mL aliquots, then placed in an insulated ice-cooled container, and analyzed within two hours of collection. Results: Average weights of female and male broodfish before spawning were 2180±159.78 g and 3060±134.99 g, respectively. The relative fecundity and egg diameter were 1029±36 eggs kg-1 and 2.42±0.05 mm, respectively. The hatching rate and embryo survival to an eyed-egg stage were respectively 76.40±2.27% and 94.76±0.42%. Sperm characteristics showed that volume was 0.60±0.12 ml kg-1 and percentage of motile sperm was 70.04±2.27%. Female broodfish weight after spawning was strongly correlated with the weight before spawned (r2 = 0.999) and absolute fecundity was also strongly correlated with female broodfish weight before spawning (r2= 0.921). Sperm concentration was moderately correlated with sperm motility (r2 = 0.556) and duration of sperm motility (r2 = 0.502). Conclusions: The gourami sago strain broodfish has suitable reproductive characteristics for the development of hatcheries. Successful natural spawning should be followed by larval weaning and feeding technology to increase growth and survival.

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Effect of sperm concentration, medium osmolality and oocyte storage on artificial fertilisation success in a myobatrachid frog (Limnodynastes tasmaniensis)

Reproduction Fertility and Development ◽

10.1071/rd02079 ◽

2004 ◽

Vol 16 (3) ◽

pp. 347 ◽

Cited By ~ 33

Author(s):

D. L. Edwards ◽

M. J. Mahony ◽

J. Clulow

Keyword(s):

Sperm Motility ◽

Sperm Concentration ◽

Storage Conditions ◽

Strongly Correlated ◽

Fertilisation Success ◽

Progressive Motility ◽

Fertilisation Rate ◽

Low Concentrations ◽

Egg Number ◽

In The Wild

The present study optimised artificial fertilisation and oocyte storage conditions in Limnodynastes tasmaniensis (Myobatrachidae). Data on general reproductive biology, the effect of sperm motility and concentration, medium osmolality and oocyte storage on artificial fertilisation success are presented. Egg number was most strongly correlated with bodyweight (r = 0.819). Sperm yield was correlated with testes weight (r = 0.827), which was strongly correlated with snout–vent length (r = 0.772). Optimal artificial fertilisation occurred in 0–7 mOsm kg–1 amphibian Ringer, similar to ranid, bufonid and hylid species. High fertilisation rates were achieved using spermatozoa with little forwards progressive motility at comparatively low concentrations (3 × 104 sperm cells mL–1) and with no relationship between percentage sperm motility and fertilisation success (correlation of fertilisation rate with sperm motility after activation: r = –0.145). Oocytes stored in 5 mOsm kg–1 solutions showed no significant decline in fertilisability after 2 h, showing that swelling of the jelly surrounding the eggs does not prevent sperm from fusing with the oocyte in this species. Fertilisability of oocytes was extended to > 4 h in medium to high osmolality solutions (124–271 mOsm kg–1). These data allow for the future use of L. tasmaniensis in developing assisted reproductive technology protocols for foam-nesting myobatrachid species, many of which are now threatened with extinction in the wild.

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Potential bleaching effects on coral reproduction

Reproduction Fertility and Development ◽

10.1071/rd15526 ◽

2016 ◽

Vol 28 (8) ◽

pp. 1061 ◽

Cited By ~ 15

Author(s):

Mary Hagedorn ◽

Virginia L. Carter ◽

Claire Lager ◽

Julio F. Camperio Ciani ◽

Alison N. Dygert ◽

...

Keyword(s):

Sperm Motility ◽

Sperm Concentration ◽

Larval Survival ◽

Ex Situ ◽

Coral Reproduction ◽

Reproductive Characteristics ◽

Steep Decline ◽

Settlement Success ◽

Fungia Scutaria ◽

Spawning Periods

Bleaching profoundly impacts coral reproduction, often for years after an event. However, detailed reproductive characteristics of coral after bleaching have not been broadly described, especially as they relate to cryopreservation. Therefore, in the present study we measured several reproductive characteristics in coral in Kaneohe Bay, Hawaii, for two species, namely Fungia scutaria and Montipora capitata, during the bleaching period of 2014 and 2015. We examined spawning periods, egg morphometry, sperm concentration, fresh and cryopreserved sperm motility exposed to different concentrations of dimethyl sulfoxide, time of first cleavage, larval survival with fresh and cryopreserved spermatozoa, infection success and settlement success. Many of these reproductive parameters were reduced in 2015, especially sperm motility. Once the reduced-motility spermatozoa from 2015 post-bleach were cryopreserved, there was a steep decline in post-thaw viability and this would prevent any substantive further use of these samples in reproduction for conservation benefit. Worldwide, as bleaching events become more frequent, the ability to bank and conserve coral ex situ may be significantly reduced. Thus, it is imperative that while genetic diversity is still high in these populations, intensive efforts are made to bank coral species during non-bleaching periods.

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Ancient Sturgeons Possess Effective DNA Repair Mechanisms: Influence of Model Genotoxicants on Embryo Development of Sterlet, Acipenser ruthenus

International Journal of Molecular Sciences ◽

10.3390/ijms22010006 ◽

2020 ◽

Vol 22 (1) ◽

pp. 6

Author(s):

Ievgeniia Gazo ◽

Roman Franěk ◽

Radek Šindelka ◽

Ievgen Lebeda ◽

Sahana Shivaramu ◽

...

Keyword(s):

Gene Expression ◽

Dna Damage ◽

Dna Repair ◽

Embryo Development ◽

Hatching Rate ◽

Embryo Survival ◽

Dna Repair Mechanisms ◽

Acipenser Ruthenus ◽

Repair Mechanisms ◽

Sterlet Acipenser Ruthenus

DNA damage caused by exogenous or endogenous factors is a common challenge for developing fish embryos. DNA damage repair (DDR) pathways help organisms minimize adverse effects of DNA alterations. In terms of DNA repair mechanisms, sturgeons represent a particularly interesting model due to their exceptional genome plasticity. Sterlet (Acipenser ruthenus) is a relatively small species of sturgeon. The goal of this study was to assess the sensitivity of sterlet embryos to model genotoxicants (camptothecin, etoposide, and benzo[a]pyrene), and to assess DDR responses. We assessed the effects of genotoxicants on embryo survival, hatching rate, DNA fragmentation, gene expression, and phosphorylation of H2AX and ATM kinase. Exposure of sterlet embryos to 1 µM benzo[a]pyrene induced low levels of DNA damage accompanied by ATM phosphorylation and xpc gene expression. Conversely, 20 µM etoposide exposure induced DNA damage without activation of known DDR pathways. Effects of 10 nM camptothecin on embryo development were stage-specific, with early stages, before gastrulation, being most sensitive. Overall, this study provides foundational information for future investigation of sterlet DDR pathways.

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Relationships between reproductive characteristics in male Vimba vimba L. and the effects of osmolality on sperm motility

Theriogenology ◽

10.1016/j.theriogenology.2010.02.017 ◽

2010 ◽

Vol 74 (2) ◽

pp. 317-325 ◽

Cited By ~ 14

Author(s):

S.M.H. Alavi ◽

P. Kozak ◽

A. Hatef ◽

J. Hamackova ◽

O. Linhart

Keyword(s):

Sperm Motility ◽

Reproductive Characteristics

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OPTIMAL ELECTROPORATION CONDITION FOR SPERM MEDIATED GENE TRANSFER IN STRIPPED CATFISH (Pangasionodon hypophthalmus)

Indonesian Aquaculture Journal ◽

10.15578/iaj.5.1.2010.1-10 ◽

2010 ◽

Vol 5 (1) ◽

pp. 1

Author(s):

Raden Roro Sri Pudji Sinarni Dewi ◽

Alimuddin Alimuddin ◽

Agus Oman Sudrajat ◽

Komar Sumantadinata ◽

Sularto Sularto

Keyword(s):

Sperm Motility ◽

Fluorescent Protein ◽

Pulse Length ◽

Transgenic Fish ◽

Pulse Number ◽

Pulse Interval ◽

Hatching Rate ◽

Exogenous Dna ◽

Motility Of Sperm ◽

Green Fluorescent

The success of transgenic fish production has been achieved through eggs fertilization using electroporated sperms carrying exogenous DNA. This study was conducted in order to obtain the optimal electroporation condition for stripped catfish sperm. A plasmid containing green fluorescent protein (GFP) gene driven by carp β-actin promoter was transferred into sperm using electrophoresis method towards transgenic stripped catfish (Pangasionodon hypophthalmus) production. Electroporation was carried out using square wave shock with pulse length of 30 ms and pulse interval of 0.1 sec. Treatments are combination between voltage (50 V, 75 V, and 100 V) and pulse number (1 and 3). Exogenous DNA concentration used was 10 μg/mL of Tris-EDTA. Results showed that increasing the voltage from 50 to 100 decreased sperm motility, while pulse number did not affect sperm motility. Voltage of 50 gave the best motility of sperm, although sperm viability relatively similar between treatments and control except at 100 V with 3 pulses number. Further, electroporation-treated sperms were able to fertilize eggs. Higher hatching rate of eggs was obtained in electroporation treatment at 50 V with pulse number of 1 and 3. The persistence of transferred GFP was detected in electroporated and incubated sperms (control). However, GFP was only detected in larvae from eggs that were fertilized by electroporated sperm. Thus, electroporation could be applied to produce transgenic stripped catfish.

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Correlation Between Ultrasonic Testicular Volume and Seminal Fluid Analysis in Men with Infertility

European Journal of Medical and Health Sciences ◽

10.24018/ejmed.2021.3.1.541 ◽

2021 ◽

Vol 3 (1) ◽

pp. 103-107

Author(s):

Sadiq Abu ◽

Hadijat O. Kolade-Yunusa ◽

Terkaa Atim ◽

F. Ehimatie Obakeye ◽

Nuhu K. Dakum

Keyword(s):

Sperm Motility ◽

Teaching Hospital ◽

Seminal Fluid ◽

Sperm Concentration ◽

Testicular Volume ◽

Seminiferous Tubules ◽

Testicular Function ◽

Semen Volume ◽

Significant Difference ◽

The Mean

Background: Infertility is a major cause of marital disharmony in Nigeria because of the high premium placed on childbearing. Unfortunately, the blame is on the woman most times in Nigeria. Seminiferous tubules comprise 80-90% of testicular mass. Thus, the testicular volume is an index of spermatogenesis. Therefore, accurate testicular volume will help in assessing testicular function if there is no obstruction. This study was conducted to evaluate the correlation between testicular volume, measured by ultrasound and conventional sperm parameters (semen volume, sperm concentration, sperm motility and sperm morphology) in men with infertility presenting to Urology Division, University of Abuja Teaching Hospital, Gwagwalada Abuja. Methodology: This was a descriptive cross sectional study, male subjects with infertility presenting to University of Abuja Teaching Hospital were recruited into the study. The testicular volume of all the subjects was measured by ultrasonography. The semen samples were collected by the process of masturbation on day five of sexual abstinence and analyzed according to WHO criteria 2010. Collated data were analyzed using SPSS version 20.0. P-value<0.05 was taken as statistically significant. Results: The average total testicular volume (TTV) of men with infertility was 19.83 ml while mean testicular volume (MTV) was 10.24 ml. There was a statistically significant difference between MTV and semen volume (r=0.391, p=0.000), and sperm concentration (r=0.639, p=0.000). There was a weak and insignificant correlation between MTV and sperm motility and MTV and morphology (r=0.216, p=0.055 and r=0.076, p=0.502) respectively. Linear regression analysis showed significant impact of MTV (P<0.001) on sperm concentration with a critical MTV of ≤10ml.0 indicating poor testicular function (severe oligospermia) Conclusion: The mean ultrasonic testicular volume in men with infertility in this study was 10.24ml and there was a significant positive correlation of MTV with semen volume and sperm concentration. Although, there was correlation of MTV with sperm motility and morphology, this was not significant. The critical mean ultrasonic testicular volume indicating poor testicular function (severe oligospermia) was found to be 10ml. This study has shown that the mean ultrasonic testicular volume can be a pointer to semen volume and sperm concentration in evaluating men with infertility.

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Evaluating the ability of semen cryopreservation of Blance Blue Belge bull cattle in Vietnam

Vietnam Journal of Biotechnology ◽

10.15625/1811-4989/16332 ◽

2021 ◽

Vol 19 (2) ◽

pp. 237-244

Author(s):

Nguyen Huu Duc ◽

Pham Thu Giang ◽

Tran Thi Binh Nguyen ◽

Nguyen Thi Mai ◽

Bui Dai Phong

Keyword(s):

Sperm Motility ◽

Sperm Concentration ◽

Joint Stock Company ◽

Stock Company ◽

Cattle Breeding ◽

Semen Cryopreservation ◽

Semen Collection ◽

Frozen Semen ◽

Semen Volume ◽

Fresh Semen

The objective of this study was to determine the semen cryopreservation capacity of BBB bulls in Hanoi-Vietnam. Research conducted on the fresh semen collected from 05 BBB bulls. Results showed that semen color was normal (milky white, ivory white, ivory yellow), semen volume ranged from 6.35 mL to 7.48 mL (P <0.05), initial motility of semen ranged from 80.53% to 82.92% (P <0.05), sperm concentration in semen ranged from 1.02 x 109 sperms/ml to 1.12 x 109 sperms/mL (P <0.05), abnormal sperm ratio ranged from 6.45% to 8.12% (P <0.05), alive sperm ratio ranged from 76.34% to 82.97% (P <0.05), sperm motility after thawing from straw semen ranged from 71.33% to 75.92% (P<0.05). In conclusion, successfully semen collection from 05 breeding BBB bulls at Hanoi Cattle Breeding Joint Stock Company, semen samples had normal color and good quantity and quality, suitable for production of frozen semen; and semen cryopreservation of straws of the 05 bull BBB semen mentioned at -196oC, sperm motility after freezing-thawing reached the economic and technical norms of 675/2014 of the Ministry of Agriculture and Rural Development.

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The Effect of Cyperus esculentus on Sperm Function Parameters in Prepubertal Mice as a Model for Human

Baghdad Science Journal ◽

10.21123/bsj.7.1.389-393 ◽

2010 ◽

Vol 7 (1) ◽

pp. 389-393 ◽

Cited By ~ 3

Author(s):

Baghdad Science Journal

Keyword(s):

Body Weight ◽

Sperm Motility ◽

Sperm Concentration ◽

Sperm Function ◽

Alcoholic Extract ◽

Cyperus Esculentus ◽

Alcohol Extract ◽

The Mean ◽

Group 2 ◽

And Control

The objective of this work was to study the effect of oral administration of Cyperus esculentus (CE) and its alcoholic extract on sperm function parameters in prepubertal mice as a model for human .The animals were divided into three groups each contains 6 animals .Group 1 was treated with 150 mg/ kg body weight /day of crude CE, group 2 was treated with same dose of alcohol extract of CE and group 3 regarded as control throughout six weeks period. The results showed a significant (p> 0.05) increase in the mean of sperm concentration ,sperm motility percent and progressive sperm motility between treated groups and control . There was no differences among groups in the mean of sperm normal morphology and sperm viability . No significant differences was recorded in the mean of body weight among groups throughout the study. The results revealed that the administration of Cyperus esculentus may enhance certain sperm characters in prepubertal mice without affecting body weight.

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Vitrification of In Vitro Produced Porcine Blastocysts: Influence of Cryoprotectants Toxicity and Embryo Age

Acta Scientiae Veterinariae ◽

10.22456/1679-9216.93774 ◽

2019 ◽

Vol 47 (1) ◽

Author(s):

Lain Uriel Ohlweiler ◽

Joana Claudia Mezzalira ◽

Alceu Mezzalira

Keyword(s):

Cell Number ◽

Lower Percentage ◽

Toxicity Tests ◽

Hatching Rate ◽

Embryo Viability ◽

Embryo Survival ◽

Porcine Embryo ◽

Negative Role ◽

Cryoprotectant Solution

Background: Porcine embryos are sensible to all assisted reproduction manipulations, especially the ones that involve cryopreservation. Despite the high cryoprotectant concentrations routinely applied, vitrification is the most effective technique to date. These substances toxicity can also play a negative role in embryo viability. During in vitro porcine embryo production, the speed of development is often unevenly distributed. It is possible that their development speed, affects embryo tolerance to cryoprotectants. This study aimed to evaluate the toxicity of porcine embryos of days 5 or 6 of culture to cryoprotectant agents; as well as to assess embryo survival to vitrification.Material, Methods & Results: Parthenogenetic porcine blastocysts and expanded blastocysts of days 5 and 6 of culture were exposed to toxicity tests (experiments 1 and 2) and vitrification (experiment 3) using different protocols. In the first experiment, three different cryoprotectants were used (Dimethyl sulfoxide - DMSO, Ethylene glycol – EG, and Sucrose - SUC), combined in three different associations (G1: 15% EG + 15% DMSO with 0.5M SUC; G2: 16% EG + 16% DMSO with 0.4M SUC; G3: 18% EG + 18% DMSO with 0.5M SUC). In the fresh Control, embryos of day 6 are more sensible than the ones of day 5, whom showed a lower hatching rate (39.7 vs. 60.8%). After the toxicity (Experiment 1) test, the G1 showed better expansion rates in day 6 (50.0 vs 31.0 and 3.6% for G2 and G3) and higher hatching of day 6 compared to G2 and G3 (23.2, vs. 8.6 and 0.0% for G2 and G3). The fresh non hatched embryos at day 8, derived at day 6, had a lower percentage of cells with cleaved caspase-3 (20.2%) compared with the G1 (30.5%), G2 (31.4%) and G3 (30.5%). The hatched embryos of day 5 from G2 had lower total cell number (TCN) compared with the day 6 hatched embryos, whereas in G1 the TCN was not affected. The second experiment compared EG combined to one of these three extracellular cryoprotectants: Polyvinylpyrrolidone/sucrose/trehalose (respectively groups: PVP, SUC, TRE). The group SUC has raised the best results for day 5 embryos, whereas for day 6 embryos SUC and TRE were both best. The third experiment tested four vitrification protocols, being P1: EG+DMSO+TRE/warming with SUC; P2: EG+DMSO+TRE/warming TRE; P3: EG+TRE/ warming SUC; P4: EG+TRE/warming TRE. The expansion of vitrified day 5 embryos was higher in the P1 (20.0%) in comparison with the other three groups (4.3, 4.3 and 4.4% for P2, P3 and P4, respectively), with no difference for their hatching rates, been it lower comparing to the Control. Day 6 embryos showed no difference in expansion and hatching for the vitrified groups, been them lower than the Control.Discussion: Embryos obtained on day 6 are more sensible than the ones of day 5, fact observed when the embryos were exposed to cryoprotectant solution, as well by the behavior of the no treated Control embryos. The toxicity increases as it does the concentration of intracellular cryoprotectant, where over 16% of the intracellular cryoprotectors already affected the day 6 embryos development. For the day 5 embryos however, 15 or 16% of the intracellular cryoptrotectors, had similar behavior to the embryos. For the extracellular solutions, however, it is variable according the embryos development speed. Indeed, it is necessary to adjust the cryoprotectors to be used to cryopreserve porcine in vitro produced embryos obtained at days 5 and 6 of culture.

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Effect of SOYXYL as a Dietary Protected Protein Supplement on the Reproductive Performance of Simmental Bulls

ANIMAL PRODUCTION ◽

10.20884/1.jap.2020.22.1.41 ◽

2020 ◽

Vol 22 (1) ◽

pp. 1-8

Author(s):

Bambang Waluyo Hadi Eko Prasetiyono ◽

Widiyanto Widiyanto ◽

Yon Soepri Ondho ◽

Agung Subrata ◽

Hermawan Setyo Widodo

Keyword(s):

Sperm Motility ◽

Plasma Protein ◽

Body Weight Gain ◽

Average Daily Gain ◽

Sperm Concentration ◽

Dietary Supplementation ◽

Blood Protein ◽

Protein Supplement ◽

Plasma Urea ◽

Total Plasma Protein

The study aimed to evaluate the effect of SOYXYL as one of the dietary protected protein supplement product in the rations on the reproduction performance of Simmental bulls. Twelve 5 years-old Simmental bulls were allotted to 4 treatment groups with 3 replications in each, i.e.: T0= control ration (elephant grass + 5 kg/head/day commercial concentrate), T1= control ration + 150 g protected protein supplement SOYXYL (3% of concentrate), T2 = control ration + 300 g SOYXYL (6% of concentrate) and T3= control ration + 450 g SOYXYL (9% of concentrate). The experiment lasted for 90 days. At the end of the experiment, semen and blood were collected and body weight gain was measured. The variables measured included sperm motility, sperm concentration, blood protein, blood urea and the concentration of testosterone. Results showed that SOYXYL supplementation, particularly at the levels of 3 and 6%, increased the total plasma protein of bulls. SOYXYL supplementation at 3% from concentrate resulted in increased plasma urea concentration. Feeding SOYXYL, particularly at the levels of 6 and 9%, increased the average daily gain of bulls as compared to control. Feeding 3% SOYXYL increased, but at the levels of 6 and 9% decreased the concentration of sperm of bulls. The concentration of testosterone was higher in the bulls received 3 and 6% SOYXYL in the rations. Dietary supplementation of SOYXYL increased the sperm motility of Simmental bulls. In conclusion, dietary supplementation of SOYXYL at the level of 3% increased plasma protein, testosterone, and sperm concentrations as well as sperm motility. However, supplementation of SOYXYL at 6 and 9% from rations decreased the concentrations of sperm and testosterone.

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