Presentation of cytokine profile in relation to oxidative stress parameters in patients with severe COVID-19: a case-control pilot study

Introduction: COVID-19 can be worsened by hyper-production of cytokines accompanied by increased level of oxidative stress. The aim of this study was to investigate the correlation between a set of cytokines and the markers of the oxidative stress. Methods: The levels of cytokines IL-2, IL-4, IL-6, IL8, IL-10, VEGF, IFN-γ, TNF-α, IL-1α, MCP-1 and EGF were determined by using High Sensitivity Evidence Investigator™ Biochip Array technology. The oxidative stress parameters (d-ROM, PAT, OS index) were measured in serum on FRAS5 analytical photometric system. Results: IL-6, IL-8, IL-10, VEGF, MCP-1 and EGF were significantly higher (p<0.05) in the patients with severe COVID-19 with increased levels of IL-2, IFN-y, TNF-α and IL-1α. The d-ROM, OS index, and PAT were significantly higher (p<0.05) in severe COVID-19 patients. IL-6 demonstrated the strongest correlation with all of the markers of the oxidative stress, d-ROM (r=0.9725, p=0.0001), PAT (r=0.5000, p=0.0001) and OS index (r=0.9593, p=0.012). Similar behavior was evidenced between IFN-y and d-ROM (r=0.4006, p=0.0001), PAT (r=0.6030, p=0.0001) and OS index (r=0.4298, p=0.012). Conclusion: The oxidative stress markers show good correlation with the tested cytokines which can be measured at the beginning of the disease in a primary care setting to predict the course of COVID-19.

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Presentation of cytokine profile in relation to oxidative stress parameters in patients with severe COVID-19: an observational pilot study

F1000Research ◽

10.12688/f1000research.55166.1 ◽

2021 ◽

Vol 10 ◽

pp. 719

Author(s):

Marija Petrushevska ◽

Dragica Zendelovska ◽

Emilija Atanasovska ◽

Aleksandar Eftimov ◽

Katerina Spasovska

Keyword(s):

Oxidative Stress ◽

Care Setting ◽

High Sensitivity ◽

Photometric System ◽

Oxidative Stress Parameters ◽

Stress Markers ◽

Tnf Α ◽

Array Technology ◽

Ifn Γ ◽

Stress Parameters

Introduction: COVID-19 can be worsened by hyper-production of cytokines accompanied by increased level of oxidative stress. The aim of this study was to investigate the correlation between a set of cytokines and the markers of the oxidative stress. Methods: The levels of cytokines IL-2, IL-4, IL-6, IL8, IL-10, VEGF, IFN-γ, TNF-α, IL-1α, MCP-1 and EGF were determined by using High Sensitivity Evidence Investigator™ Biochip Array technology. The oxidative stress parameters (d-ROM, PAT, OS index) were measured in serum on FRAS5 analytical photometric system. Results: IL-6, IL-8, IL-10, VEGF, MCP-1 and EGF were significantly higher (p<0.05) in the patients with severe COVID-19 with increased levels of IL-2, IFN-g, TNF-a and IL-1α. The d-ROM, OS index, and PAT were significantly higher (p<0.05) in severe COVID-19 patients. IL-6 demonstrated the strongest correlation with all of the markers of the oxidative stress, d-ROM (r=0.9725, p=0.0001), PAT (r=0.5000, p=0.0001) and OS index (r=0.9593, p=0.012). Similar behavior was evidenced between IFN-g and d-ROM (r=0.4006, p=0.0001), PAT (r=0.6030, p=0.0001) and OS index (r=0.4298, p=0.012). Conclusion: The oxidative stress markers show good correlation with the tested cytokines which can be measured at the beginning of the disease in a primary care setting to predict the course of COVID-19.

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Oxidative stress parameters and inflammatory and immune mediators as markers of the severity of sepsis

The Journal of Infection in Developing Countries ◽

10.3855/jidc.7585 ◽

2016 ◽

Vol 10 (10) ◽

pp. 1045-1052 ◽

Cited By ~ 7

Author(s):

Isil Bavunoglu ◽

Habibe Genc ◽

Dildar Konukoglu ◽

Hayriye Cicekci ◽

Volkan Sozer ◽

...

Keyword(s):

Oxidative Stress ◽

Severe Sepsis ◽

Adhesion Molecule ◽

Inflammatory Mediators ◽

Paraoxonase 1 ◽

Pon1 Activity ◽

Enzyme Linked Immunosorbent Assay ◽

Oxidative Stress Parameters ◽

Tnf Α ◽

Stress Parameters

Introduction: Sepsis is a complex inflammatory syndrome with diverse etiology and wide spectrum of severity. The aim of this study was to investigate whether inflammatory mediators, in comparison with oxidative parameters, are associated with severity of sepsis. Methodology: Plasma neopterin, adenosine deaminase (ADA), vascular cell adhesion molecule (VCAM), intracellular adhesion molecule (ICAM), interleukin (IL)-1, IL-6, and tumor necrosis factor alpha (TNF-α), as inflammatory mediators, and serum nitric oxide (NOx), nitrotyrosine (NT), oxidized LDL (oxLDL) levels, serum paraoxonase 1 (PON1) activity, and erythrocyte glutathione (GSH) levels as oxidative stress parameters of 12 patients with mild sepsis, 25 patients with severe sepsis, and 20 healthy control subjects were evaluated. NOx, GSH levels and PON1 activity were determined by colorimetric methods, whereas neopterin, VCAM, ICAM, IL-1, IL-6, TNF-α, NT, and oxLDL levels were measured by enzyme-linked immunosorbent assay (ELISA). Results: All parameters in mild and severe sepsis were significantly different from those of healthy subjects, except ADA activities. Patients with severe sepsis exhibited higher IL-6, TNF-α, NT, and oxLDL levels than patients with mild sepsis. GSH (98%, 98%), oxLDL (98%, 98%), VCAM-1 (99%, 99%), and ICAM-1 (99%, 99%) have much more sensitivitiy and specificity in sepsis. Conclusions: Our results suggest that the oxidative stress and inflammatory response in patients with sepsis were increased and that serum IL-6, TNF-α, NT, and oxLDL levels were correlated with the severity of sepsis. Therefore, increases in these parameters may contribute to the dysfunction or failure of one or more organs, or even death, in sepsis.

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Influence of Synbiotics on Selected Oxidative Stress Parameters

Oxidative Medicine and Cellular Longevity ◽

10.1155/2017/9315375 ◽

2017 ◽

Vol 2017 ◽

pp. 1-8 ◽

Cited By ~ 6

Author(s):

Paulina Kleniewska ◽

Rafał Pawliczak

Keyword(s):

Oxidative Stress ◽

Lactobacillus Casei ◽

Positive Influence ◽

Control Groups ◽

Oxidative Stress Parameters ◽

Stress Markers ◽

Sh Group ◽

Synbiotic Supplementation ◽

And Control ◽

Stress Parameters

The aim of the present study was to assess synbiotic (Lactobacillus casei+ inulin) influence on oxidative stress parameters such as concentrations of malondialdehyde (MDA), hydrogen peroxide (H2O2), glutathione, and free sulfhydryl groups content. Experiments were carried out on healthy volunteers (n=32). The subjects were divided into women group (n=16) and men group (n=16) and randomly assigned to synbiotic and control groups. Blood samples were collected before synbiotic supplementation and after 7 wks, at the end of the study. The administration of synbiotic resulted in a significant decrease in MDA (p<0.01), H2O2(p<0.01), and GSSG concentrations (p<0.05) as compared with the control groups and significant increase in the concentrations of GSHt (p<0.001), GSH (p<0.01), and -SH group content (p<0.05) versus control. Synbiotics containingL. caseiplus inulin may have positive influence on selected oxidative stress markers.

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The possible alleviating effect of garlic supplement on the neural retina in a rat model of hypercholesterolemia: a histological and immunohistochemical study

European Journal of Histochemistry ◽

10.4081/ejh.2021.3322 ◽

2021 ◽

Vol 65 (4) ◽

Author(s):

Eetmad A. Arafat ◽

Eman M.I. Youssef ◽

Hanaa A. Khalaf

Keyword(s):

Oxidative Stress ◽

Microscopic Examination ◽

Electron Microscopic Examination ◽

Neural Retina ◽

Positive Control ◽

High Cholesterol Diet ◽

Electron Microscopic ◽

Oxidative Stress Parameters ◽

Tnf Α ◽

Stress Parameters

The purpose of this work was to prove that oxidative stress is the main mechanism responsible for retinal neurodegenerative changes, subsequent apoptosis, and inflammatory cytokine release in rats fed with a high cholesterol diet (HCD) and determine the role of garlic in alleviating these changes. Forty rats were equally divided into four groups: control, garlic-treated (positive control), HCD, and HCD + garlic-treated (HCD + G). By the end of the experiment (24 weeks) blood samples were collected for assessment of serum lipid profile, oxidative stress parameters, and plasma levels of IL-6 and TNF-α. Both eyes of the rats were enucleated; one was used for light microscopic examination and the other for electron microscopic examination. There was a significant increase in the levels of serum lipids, oxidative stress parameters, IL-6 and TNF-α, and area of expression of caspase-3 in the HCD group compared to both the control and HCD + G groups. Histological examination revealed degenerative changes in all layers of the neural retina in the HCD group. Garlic administration resulted in a significant improvement in the biochemical, immunohistochemical, and histological characteristics of hypercholesterolemic rats. These findings support the hypotheses that garlic has strong antioxidant, anti-apoptotic, and anti-inflammatory properties. Garlic ameliorates the neurodegenerative changes in the neural retina of hypercholesteremic rats.

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ANALYSIS OF OXIDATIVE STRESS MARKERS IN CROSSBRED COWS WITH SUBCLINICAL MASTITIS AND CONCURRENT METABOLIC AND INFECTIOUS DISEASES

International Journal on Environmental Sciences ◽

10.53390/ijes.v12i1.5 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Mohammed Khaleel Ulla ◽

A.M. Kotresh ◽

Anantha Krishna L.R ◽

Shambulingappa B.E ◽

Rudresh. B.H ◽

...

Keyword(s):

Oxidative Stress ◽

Infectious Diseases ◽

Subclinical Mastitis ◽

Clinical Mastitis ◽

Crossbred Cows ◽

Oxidative Stress Parameters ◽

Stress Markers ◽

History Of ◽

Loss Of Appetite ◽

Stress Parameters

The aim of this study was to evaluate the oxidative status in crossbred cows with subclinical mastitis and concurrent metabolic and infectious diseases. The crossbred cows were groped asGp-C (n=6) as controls,Gp-M (n=6) with clinical mastitis ,Gp-TS consists cows affected withstenosis of teat,Gp-RA with ruminal acidosis,Gp-ND with diarrhea along with dehydration and loss of appetite, Gp-RTIwith coughing, fever, decreased appetite, varying degrees of dyspnea, GpRB with a history of failure to conceive after at least two successive inseminations. oxidative stress parameters likecatalase, SOD, GPx, GSH and malondialdehyde were estimated spectrophotometrically. The antioxidant enzymes like catalase, super oxide dismutase (SOD), Glutathione Peroxidase (GPx) levels were significantly declined and the non enzymatic parameters reduced glutathione (GSH) and malondialdehyde (MDA) are significantly elevated in affected animals than in healthy animals. The results of the oxidative stress parameters indicate imbalance of antioxidant profile and oxidative stress in the animals with various disorders and previous illness along with subclinical mastitis.

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Effects of Spiruline (Spirulina platensis) Supplementation on Oxidative Stress Markers, Biochemical Characteristics, and Hematological Parameters in Rabbit (Oryctolagus cuniculus) Does

Asian Journal of Agriculture and Rural Development ◽

10.18488/journal.ajard.2021.112.163.170 ◽

2021 ◽

Vol 11 (2) ◽

pp. 163-170

Author(s):

Chongsi Margaret Mary Momo ◽

Mahamat Tahir Markhous Adam ◽

Tchoffo Herve ◽

Vemo Bertin Narcisse ◽

Ngoula Ferdinand ◽

...

Keyword(s):

Oxidative Stress ◽

Hematological Parameters ◽

Control Group ◽

Oxidative Stress Markers ◽

Feed Supplementation ◽

Biochemical Characteristics ◽

Oxidative Stress Parameters ◽

Stress Markers ◽

Sexually Mature ◽

Stress Parameters

The present study was carried out in order to evaluate the effects of feed supplementation with spiruline powder on the oxidative stress markers, biochemical characteristics, and hematological parameters in rabbit doe. Twenty-one nulliparous and sexually mature does (7–8 months old) were distributed into three groups of seven does each, comparable in terms of body weight. After a week of feeding with experimental feed, does of each group were mated. During the trial, does of group 1 (control group) were fed ad libitum with a feed free from spiruline (control feed), while those of groups 2 and 3 received the control feed supplemented with spiruline powder at 0.6 and 1.2%, respectively. Immediately after parturition, blood was collected for analyses of hematological, biochemical, and oxidative stress parameters. Studied parameters included serum concentrations of total proteins, cholesterol, aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), creatinine, and urea; malondialdehyde, reduced glutathione, catalase, and superoxide dismutase; and hematological parameters. The total protein concentration, number of white and red blood cells, platelets, hemoglobin, hematocrit, and mean corpuscular volume were significantly (P<0.05) higher in does treated compared to control. Oxidative stress parameters were comparable (P>0.05) among treatments. Hence, these results show that feed supplementation with spirulina powder can improve rabbit doe health, notably at 1.2%, since optimal results were obtained at this percentage.

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