scholarly journals Rhamno Lipids Biosurfactants from Pseudomonas aeruginosa - A Review

2018 ◽  
Vol 15 (4) ◽  
pp. 767-781 ◽  
Author(s):  
Jaciara Araújo ◽  
Juliene Rocha ◽  
Marcos Oliveira Filho ◽  
Stephanie Matias ◽  
Sérgio Oliveira Júnior ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Industrial Application ◽  
Production Cost ◽  
Low Cost ◽  
Carbon Sources ◽  
Total Production ◽  
Amphiphilic Compound ◽  
Biological Compounds ◽  
Bona Fide

Studies addressing for ecological compatible products have been increased along time, especially, on biosurfactant field. Biosurfactants are extracellular amphiphilic compound that are mainly produced by microorganisms and are classified into five main groups, including the glycolipids one. Rhamnolipids are included in the latter and are anionic biosurfactants produced predominantly by Pseudomonas aeruginosa being classified as mono- and di-rhamnolipids. In addition, their production may occur from different carbon sources, which may be obtained from renewable and low-cost residue. Therefore, it is possible to reduce the rhamnolipids production cost, since this has been the main bottleneck for replacing the chemical surfactants. In addition, to meeting a bona fide industrial application some limitations such as low productivity as well as recovery and/or purification that represent from 60 to 80% of total production cost should be improved. Therefore, this review covers different ways for producing rhamnolipids covering their application in many fields such as pharmaceutical, agricultural, petrochemical and so on; demonstrating the versatility of these biological compounds.

scholarly journals Glycerol or crude glycerol as substrates make Pseudomonas aeruginosa achieve anaerobic production of rhamnolipids

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Feng Zhao ◽  
Yuting Wu ◽  
Qingzhi Wang ◽  
Mengyao Zheng ◽  
Qingfeng Cui
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Crude Glycerol ◽  
Low Cost ◽  
Carbon Sources ◽  
Anaerobic Growth ◽  
Strain Specificity ◽  
Spectra Analysis ◽  
Anoxic Environments ◽  
Anaerobic Production

Abstract Background The anaerobic production of rhamnolipids is significant in research and application, such as foamless fermentation and in situ production of rhamnolipids in the anoxic environments. Although a few studies reported that some rare Pseudomonas aeruginosa strains can produce rhamnolipids anaerobically, the decisive factors for anaerobic production of rhamnolipids were unknown. Results Two possible hypotheses on the decisive factors for anaerobic production of rhamnolipids by P. aeruginosa were proposed, the strains specificity of rare P. aeruginosa (hypothesis 1) and the effect of specific substrates (hypothesis 2). This study assessed the anaerobic growth and rhamnolipids synthesis of three P. aeruginosa strains using different substrates. P. aeruginosa strains anaerobically grew well using all the tested substrates, but glycerol was the only carbon source that supported anaerobic production of rhamnolipids. Other carbon sources with different concentrations still failed for anaerobic production of rhamnolipids by P. aeruginosa. Nitrate was the excellent nitrogen source for anaerobic production of rhamnolipids. FTIR spectra analysis confirmed the anaerobically produced rhamnolipids by P. aeruginosa using glycerol. The anaerobically produced rhamnolipids decreased air-water surface tension to below 29.0 mN/m and emulsified crude oil with EI24 above 65%. Crude glycerol and 1, 2-propylene glycol also supported the anaerobic production of rhamnolipids by all P. aeruginosa strains. Prospects and bottlenecks to anaerobic production of rhamnolipids were also discussed. Conclusions Glycerol substrate was the decisive factor for anaerobic production of rhamnolipids by P. aeruginosa. Strain specificity resulted in the different anaerobic yield of rhamnolipids. Crude glycerol was one low cost substrate for anaerobic biosynthesis of rhamnolipids by P. aeruginosa. Results help advance the research on anaerobic production of rhamnolipids, deepen the biosynthesis theory of rhamnolipids and optimize the anaerobic production of rhamnolipids.

Immaterial Cost and Production: Maximum Production Cost Level Through Marginal Approach

2020 ◽  
Vol 8 (2) ◽  
pp. 128-149
Author(s):  
Dini Maulana Lestari
Keyword(s):  
Quantitative Study ◽  
Marginal Cost ◽  
Production Cost ◽  
Maximum Level ◽  
Production Costs ◽  
Total Production ◽  
Sugar Factory ◽  
Maximum Production ◽  
Cost Approach ◽  
South Sulawesi

This paper will discuss about the immaterial costs and production yields at one of the refined sugar factory companies in Makassar, South Sulawesi. The theory is based on the fact that Immaterial is a cost that is almsgiving, meaning costs that are outside of the basic costs of the company in producing production, so this research aims to find out: (1) what is the production cost needed to produce this production, (2) the maximum level of production at company from 2013 to 2017. This type of research is a quantitative study because it uses a questionnaire in the form of values ​​that are processed using the marginal cost approach formula. The results of the analysis show that (1) the maximum level of production costs occurred in 2016 amounting to 6,912 with an Immaterial cost of Rp. 2,481,796,800 and the total production produced is 359,077.3 tons (2) The required workforce with the total production produced is 359,077.3 tones of 180 people including the maximum production point which means that the lowest value is achieved (optimal).    

MINIMALISASI BIAYA SIMPAN DAN BIAYA SETUP PADA MULTIPLE PRODUK: SIMULASI DENGAN CAPACITATED LOT SIZING PROBLEM

2019 ◽  
Vol 4 (2) ◽  
pp. 205-214
Author(s):  
Erika Fatma
Keyword(s):  
Production Cost ◽  
Lot Sizing ◽  
Setup Time ◽  
Production Costs ◽  
Total Production ◽  
Product Cycle ◽  
Lot Size ◽  
Setup Costs ◽  
Lot Sizing Problem ◽  
Capacitated Lot Sizing

Lot sizing problem in production planning aims to optimize production costs (processing, setup and holding cost) by fulfilling demand and resources capacity costraint. The Capacitated Lot sizing Problem (CLSP) model aims to balance the setup costs and inventory costs to obtain optimal total costs. The object of this study was a plastic component manufacturing company. This study use CLSP model, considering process costs, holding costs and setup costs, by calculating product cycle and setup time. The constraint of this model is the production time capacity and the storage capacity of the finished product. CLSP can reduce the total production cost by 4.05% and can reduce setup time by 46.75%.  Keyword: Lot size, CLSP, Total production cost.

Application of Phenotype Microarray for Profiling Carbon Sources Utilization between Biofilm and Non-Biofilm of Pseudomonas aeruginosa from Clinical Isolates

2020 ◽  
Vol 21 (14) ◽  
pp. 1539-1550
Author(s):  
Nur S. Ismail ◽  
Suresh K. Subbiah ◽  
Niazlin M. Taib
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carbon Sources ◽  
Anaerobic Respiration ◽  
Regulatory System ◽  
Negative Control ◽  
High Morbidity ◽  
Metabolic Profiles ◽  
Phenotype Microarray ◽  
Diverse Environment ◽  
Phenotype Microarrays

Background: This is the fastest work in obtaining the metabolic profiles of Pseudomonas aeruginosa in order to combat the infection diseases which leads to high morbidity and mortality rates. Pseudomonas aeruginosa is a high versatility of gram-negative bacteria that can undergo aerobic and anaerobic respiration. Capabilities in deploying different carbon sources, energy metabolism and regulatory system, ensure the survival of this microorganism in the diverse environment condition. Determination of differences in carbon sources utilization among biofilm and non-biofilm of Pseudomonas aeruginosa provides a platform in understanding the metabolic activity of the microorganism. Methods: The study was carried out from September 2017 to February 2019. Four archive isolates forming strong and intermediate biofilm and non-biofilms producer were subcultured from archive isolates. ATCC 27853 P. aeruginosa was used as a negative control or non-biofilm producing microorganism. Biofilm formation was confirmed by Crystal Violet Assay (CVA) and Congo Red Agar (CRA). Metabolic profiles of the biofilm and non-biofilms isolates were determined by phenotype microarrays (Biolog Omnilog). Results and Discussion: In this study, Pseudomonas aeruginosa biofilm isolates utilized uridine, L-threonine and L-serine while non-biofilm utilized adenosine, inosine, monomethyl, sorbic acid and succinamic acid. Conclusion: The outcome of this result will be used for future studies to improve detection or inhibit the growth of P. aeruginosa biofilm and non-biofilm respectively.

scholarly journals Immobilization of Pseudomonas aeruginosa static biomass on eggshell powder for on-line preconcentration and determination of Cr (VI)

2020 ◽  
Vol 18 (1) ◽  
pp. 303-313 ◽  
Author(s):  
Aamir Rasheed ◽  
Tahseen Ghous ◽  
Sumaira Mumtaz ◽  
Muhammad Nadeem Zafar ◽  
Kalsoom Akhter ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Low Cost ◽  
Colorimetric Detection ◽  
Time Flow ◽  
Highly Sensitive ◽  
Glass Column ◽  
On Line ◽  
Preconcentration Time ◽  
Ultra Trace

AbstractIn the present work, a novel continuous flow system (CFS) is developed for the preconcentration and determination of Cr (VI) using Pseudomonas aeruginosa static biomass immobilized onto an effective and low-cost solid support of powdered eggshells. A mini glass column packed with the immobilized biosorbent is incorporated in a CFS for the preconcentration and determination of Cr (VI) from aqueous solutions. The method is based on preconcentration, washing and elution steps followed by colorimetric detection with 1,5-diphenyl carbazide in sulphuric acid. The effects of several variables such as pH, retention time, flow rate, eluent concentration and loaded volume are studied. Under optimal conditions, the CFS method has a linear range between 10 and 100 μg L-1 and a detection limit of 6.25 μg L-1 for the determination of Cr (VI). The sampling frequency is 10 samples per hour with a preconcentration time of 5 mins. Furthermore, after washing with a 0.1 M buffer (pH 3.0), the activity of the biosorbent is regenerated and remained comparable for more than 200 cycles. Scanning electron microscopy reveals a successful immobilization of biomass on eggshells powder and precipitation of Cr (VI) on the bacterial cell surface. The proposed method proves highly sensitive and could be suitable for the determination of Cr (VI) at an ultra-trace level.

scholarly journals Burkholderia thailandensis E264 as a promising safe rhamnolipids’ producer towards a sustainable valorization of grape marcs and olive mill pomace

2021 ◽  
Author(s):  
Alif Chebbi ◽  
Massimiliano Tazzari ◽  
Cristiana Rizzi ◽  
Franco Hernan Gomez Tovar ◽  
Sara Villa ◽  
...  
Keyword(s):  
Energy Source ◽  
Olive Oil ◽  
Low Cost ◽  
Carbon Sources ◽  
Burkholderia Thailandensis ◽  
Rhamnolipid Production ◽  
Key Points ◽  
Wide Range ◽  
Long Chain ◽  
Olive Mill

Abstract Within the circular economy framework, our study aims to assess the rhamnolipid production from winery and olive oil residues as low-cost carbon sources by nonpathogenic strains. After evaluating various agricultural residues from those two sectors, Burkholderia thailandensis E264 was found to use the raw soluble fraction of nonfermented (white) grape marcs (NF), as the sole carbon and energy source, and simultaneously, reducing the surface tension to around 35 mN/m. Interestingly, this strain showed a rhamnolipid production up to 1070 mg/L (13.37 mg/g of NF), with a higher purity, on those grape marcs, predominately Rha-Rha C14-C14, in MSM medium. On olive oil residues, the rhamnolipid yield of using olive mill pomace (OMP) at 2% (w/v) was around 300 mg/L (15 mg/g of OMP) with a similar CMC of 500 mg/L. To the best of our knowledge, our study indicated for the first time that a nonpathogenic bacterium is able to produce long-chain rhamnolipids in MSM medium supplemented with winery residues, as sole carbon and energy source. Key points • Winery and olive oil residues are used for producing long-chain rhamnolipids (RLs). • Both higher RL yields and purity were obtained on nonfermented grape marcs as substrates. • Long-chain RLs revealed stabilities over a wide range of pH, temperatures, and salinities

Process Simulation and Economic Analysis of Rhamnolipid Production by Pseudomonas aeruginosa Using Oil Palm Empty Fruit Bunch as Substrate

2021 ◽  
Vol 104 ◽  
pp. 103-112
Author(s):  
Andrea Gilang Fauzi ◽  
Cut Nanda Sari ◽  
Andre Fahriz Perdana Harahap ◽  
Muhammad Yusuf Arya Ramadhan ◽  
Misri Gozan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Oil Palm ◽  
Process Simulation ◽  
Production Cost ◽  
Economic Assessment ◽  
Industrial Applications ◽  
Empty Fruit Bunch ◽  
Rhamnolipid Production ◽  
Surfactant Production ◽  
Purification Methods

Surfactants are amphiphilic molecules that have been used in widely used as emulsifier and cleaning agent in various industries. These broad industrial applications made the global surfactant production increased annually. In 2020, the amount of global surfactant production estimated would reach more than 24 million per year. However, the currently used surfactants are synthetically produced from chemical or petroleum-derived products, so it is often toxic and non-biodegradable. Rhamnolipids are “surfactant like” molecules produced by Pseudomonas aeruginosa, which could be alternatives for more environmentally friendly surfactants. The use of rhamnolipids is quite limited due to its expensive production cost. The production cost of rhamnolipid could be reduced using by agro-industrial by-product as a substrate. One of the abundant agro-industrial by-products in Indonesia that can be used as a substrate for rhamnolipid production is Oil Palm Empty Fruit Bunch (OPEFB). This study employed SuperPro Designer v9.5 to performed process simulation and economic assessment of rhamnolipid production using OPEFB as a substrate through two different purification methods, which are solvent extraction and chromatography adsorption. Based on the process simulation that has been done, the process that used adsorption chromatography purification methods more efficient in terms of the usage of the feedstock and energy. The economic assessment also showed that these methods were more profitable and economically attractive, with the value of NPV 11.400.000 USD, IRR 11,64%, and Payback Period 6,21 years.

scholarly journals Economic viability of the piauçu Leporinus macrocephalus (Garavello & Britski, 1988) production

2004 ◽  
Vol 61 (2) ◽  
pp. 228-233 ◽  
Author(s):  
Leonardo Susumu Takahashi ◽  
Flavio Daolio Gonçalves ◽  
Janessa Sampaio de Abreu ◽  
Maria Inez Espagnoli Geraldo Martins ◽  
Antonio Carlos Manduca Ferreira
Keyword(s):  
Production Cost ◽  
Economic Valuation ◽  
Relative Magnitude ◽  
Productive Efficiency ◽  
Economic Viability ◽  
Selling Price ◽  
Total Production ◽  
Fish Production ◽  
Present Value ◽  
Fish Farms

Brazilian fish farms presented an accelerated development during the early 90's, mainly because of the increase in fee-fishing operations. To meet the demand of this market, fish production and supply became excessive and, as a consequence, the number of fee-fishing operations, farmers and the final selling price, decreased. This study analyzes the technical aspects, production cost, profitability and economic viability of the production of piauçu (L. macrocephalus) in ponds, based on information from a rural property. Feeding and fingerling costs amount to approximately 47.1% of the total production cost, representing together with the final selling price the most important factor affecting profitability. The payback period was 8.3 years, the liquid present value US$ 291.07, the internal return margin 9%, and the income-outcome ratio was 1.01, which represents an unattractive investment as a projection based on current conditions. The improvement in productive efficiency enhances the economic valuation index, and that the relative magnitude of cost and income are the most important points for the economic viability of the studied farm.

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