scholarly journals Cellulosic Ethanol Potential of Feedstocks Grown on Marginal Lands

2018 ◽  
Vol 61 (6) ◽  
pp. 1775-1782
Sun Min Kim ◽  
DoKyoung Lee ◽  
Santanu Thapa ◽  
Bruce S. Dien ◽  
Mike E. Tumbleson ◽  

Abstract. To examine the chemical composition and ethanol production of feedstocks grown on marginal lands, prairie cordgrass and switchgrass from waterlogged land, saline land, and saline water irrigated land were evaluated. Samples were pretreated using 1% w w-1 dilute acid at 160°C for 10 min, and simultaneous saccharification and cofermentation was conducted using industrial engineered . Samples grown on land irrigated with saline water had 2.8-fold higher total ash content compared to the other types of land, resulting in lower carbohydrate concentrations. Yeast fermented glucose and xylose simultaneously; almost all of the sugars were consumed, indicating that salts present in biomass ash did not inhibit yeast performance. Ethanol production from the waterlogged and saline lands was 2,500 to 4,700 L ha-1, which is comparable to that of samples grown on other agricultural lands. Prairie cordgrass and switchgrass grown on marginal lands could be potential feedstocks for cellulosic biofuel. Keywords: Irrigation, Marginal land, Prairie cordgrass, Saline, Simultaneous saccharification and cofermentation, Switchgrass, Waterlogging.

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Agustin Krisna Wardani ◽  
Aji Sutrisno ◽  
Titik Nur Faida ◽  
Retno Dwi Yustina ◽  
Untung Murdiyatmo

Background. Oil palm trunk (OPT) with highly cellulose content is a valuable bioresource for bioethanol production. To produce ethanol from biomass, pretreatment is an essential step in the conversion of lignocellulosic biomass to fermentable sugars such as glucose and xylose. Several pretreatment methods have been developed to overcome biomass recalcitrance. In this study, the effects of different pretreatment methods such as alkali pretreatment, microwave-alkali, and alkaline peroxide combined with autoclave on the lignocellulosic biomass structure were investigated. Moreover, ethanol production from the treated biomass was performed by simultaneous saccharification and cofermentation (SSCF) under different temperatures, fermentation times, and cell ratios of Saccharomyces cerevisiae NCYC 479 and pentose-utilizing yeast, Pichia stipitis NCYC 1541. Results. Pretreatment resulted in a significant lignin removal up to 83.26% and cellulose released up to 80.74% in treated OPT by alkaline peroxide combined with autoclave method. Enzymatic hydrolysis of treated OPT resulted in an increase in fermentable sugar up to 93.22%. Optimization of SSCF by response surface method showed that the coculture could work together to produce maximum ethanol (1.89%) and fermentation efficiency (66.14%) under the optimized condition. Conclusion. Pretreatment by alkaline peroxide combined with autoclave method and SSCF process could be expected as a promising system for ethanol production from oil palm trunk and various lignocellulosic biomass.

2021 ◽  
Vol 14 (1) ◽  
Shannon M. Hoffman ◽  
Maria Alvarez ◽  
Gilad Alfassi ◽  
Dmitry M. Rein ◽  
Sergio Garcia-Echauri ◽  

Abstract Background Future expansion of corn-derived ethanol raises concerns of sustainability and competition with the food industry. Therefore, cellulosic biofuels derived from agricultural waste and dedicated energy crops are necessary. To date, slow and incomplete saccharification as well as high enzyme costs have hindered the economic viability of cellulosic biofuels, and while approaches like simultaneous saccharification and fermentation (SSF) and the use of thermotolerant microorganisms can enhance production, further improvements are needed. Cellulosic emulsions have been shown to enhance saccharification by increasing enzyme contact with cellulose fibers. In this study, we use these emulsions to develop an emulsified SSF (eSSF) process for rapid and efficient cellulosic biofuel production and make a direct three-way comparison of ethanol production between S. cerevisiae, O. polymorpha, and K. marxianus in glucose and cellulosic media at different temperatures. Results In this work, we show that cellulosic emulsions hydrolyze rapidly at temperatures tolerable to yeast, reaching up to 40-fold higher conversion in the first hour compared to microcrystalline cellulose (MCC). To evaluate suitable conditions for the eSSF process, we explored the upper temperature limits for the thermotolerant yeasts Kluyveromyces marxianus and Ogataea polymorpha, as well as Saccharomyces cerevisiae, and observed robust fermentation at up to 46, 50, and 42 °C for each yeast, respectively. We show that the eSSF process reaches high ethanol titers in short processing times, and produces close to theoretical yields at temperatures as low as 30 °C. Finally, we demonstrate the transferability of the eSSF technology to other products by producing the advanced biofuel isobutanol in a light-controlled eSSF using optogenetic regulators, resulting in up to fourfold higher titers relative to MCC SSF. Conclusions The eSSF process addresses the main challenges of cellulosic biofuel production by increasing saccharification rate at temperatures tolerable to yeast. The rapid hydrolysis of these emulsions at low temperatures permits fermentation using non-thermotolerant yeasts, short processing times, low enzyme loads, and makes it possible to extend the process to chemicals other than ethanol, such as isobutanol. This transferability establishes the eSSF process as a platform for the sustainable production of biofuels and chemicals as a whole.

2010 ◽  
Vol 171-172 ◽  
pp. 261-265
Zhuang Zuo ◽  
Xiu Shan Yang

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH,50°C,48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% delignification, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous saccharification and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

2021 ◽  
Vol 3 (01) ◽  
pp. 1-9
Ibnu Rizki Perdana ◽  
Mieke Rochimi S ◽  
Pujawati Suryatmana

Chili (Capsicum annum L) was one of the vegetable commodities that have a significant  economic value. Farmland narrowed due to land conversion led to a shift of fertile agricultural lands into marginal lands such as saline land. This research aimed to determine respond of chili plant growth  to salinity stress in Inceptisols. The experiment was conducted in August-October 2020 with located at Ciparanje Experimental field of Faculty of Agriculture, Padjadjaran University, Jatinangor District, Sumedang Regency. This research used randomized block design using four level treatment of NaCl concentration: 0 dS/m (a0), 2 dS/m (a1), 4 dS/m (a2), and 6 dS/m (a3). The result showed that there was no significant between treatment soil salinization of plant growth parameters like plant height and shoot root ratio but significant of number of leaves at 7 day after plant and canopy width at 28 day after plant on treatment salinization 6 dS/m.

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