scholarly journals Molecular identification of coprophilous microfungi from Banyumas District, Central Java, Indonesia

2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Aris Mumpuni ◽  
Adi Amurwanto ◽  
Daniel Joko Wahyono

Abstract. Mumpuni A, Amurwanto A, Wahyono DJ. 2021. Molecular identification of coprophilous microfungi from Banyumas District, Central Java, Indonesia. Biodiversitas 22: 1550-1557. Coprophilous microfungi are a group of fungi that are ecologically interesting in relation to herbivores. These fungi play a predominant role in the decomposition of organic matter, in which the organic matter passes through a series of events involving mechanical degradation, as well as physical and biological processes. The role of coprophilous fungi as the main decomposers of the lignocellulosic material of herbivorous animal waste, which is widespread in nature, is very important. Previous research on the inventory and identification of coprophilous fungi in the Banyumas district has been limited to macroscopic genera, so the results have not been able to provide a comprehensive picture of the presence of coprophilous fungi in the region. Identification of the types of microscopic coprophilous fungi that live in herbivorous animal waste, such as lignocellulosic material, is necessary to understand the taxonomy of these fungi. This study aimed to investigate and identify microscopic coprophilous fungi obtained in the Banyumas district of Central Java, Indonesia. Based on the purposive random sampling method, the obtained fungi were analyzed using the molecular methods of DNA isolation, gene amplification, DNA sequencing and phylogenetic analysis of fungal cultures. The following species and genera were identified: Ceriporia lacerata, Trichosporon insectorum, Lentinus squarrosulus, Fusarium sp., Aspergillus sp., and Trichosporon sp.

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Aris Mumpuni ◽  
Nuraeni Ekowati ◽  
Daniel Joko Wahyono

Abstract. Mumpuni A, Ekowati N, Wahyono DJ. 2020. The existence of coprophilous macrofungi in Banyumas, Central Java, Indonesia. Biodiversitas 21: 282-289. Coprophilous fungi are cosmopolitan that inhabiting herbivorous animal feces. Some of them are edible mushrooms, as well as hallucinogenic psychotropic fungi that can be used in the pharmaceutical industry. Studies on coprophilous fungi in Indonesia have not been widely carried out. Tropical climate conditions in Indonesia including in the Ex- Residence of Banyumas, Central Java Province, Indonesia support the growth and spread of coprophilous fungi and are promoted by the spread of herbivorous livestock evenly in almost all regions that always provide suitable dung substrates for the fungal habitat. Based on this background, the purpose of this preliminary study was to obtain coprophilous fungi genera and find out their dominance in the area. This research used survey method with purposive random sampling and focused on macroscopic fungi. The obtained fungi were identified as macro and micro morphologically. From this study, there were 12 genera which were Panaeolus, Coprinopsis, Stropharia, Tricholoma, Lycoperdon, Ascobolus, Rhodocybe, Conocybe, Bolbitius, Leucocoprinus, Mycena, and Hypoloma. The dominance index of the coprophil fungal genera in the ex-residency Banyumas was 0.329; and the coprophil fungi obtained with the highest frequency of occurrence were Coprinopsis (34.4%) and Panaeolus (30.1%).


2012 ◽  
Vol 9 (12) ◽  
pp. 5181-5197 ◽  
Author(s):  
C. Moni ◽  
D. Derrien ◽  
P.-J. Hatton ◽  
B. Zeller ◽  
M. Kleber

Abstract. Physical fractionation is a widely used methodology to study soil organic matter (SOM) dynamics, but concerns have been raised that the available fractionation methods do not well describe functional SOM pools. In this study we explore whether physical fractionation techniques isolate soil compartments in a meaningful and functionally relevant way for the investigation of litter-derived nitrogen dynamics at the decadal timescale. We do so by performing aggregate density fractionation (ADF) and particle size-density fractionation (PSDF) on mineral soil samples from two European beech forests a decade after application of 15N labelled litter. Both density and size-based fractionation methods suggested that litter-derived nitrogen became increasingly associated with the mineral phase as decomposition progressed, within aggregates and onto mineral surfaces. However, scientists investigating specific aspects of litter-derived nitrogen dynamics are pointed towards ADF when adsorption and aggregation processes are of interest, whereas PSDF is the superior tool to research the fate of particulate organic matter (POM). Some methodological caveats were observed mainly for the PSDF procedure, the most important one being that fine fractions isolated after sonication can not be linked to any defined decomposition pathway or protective mechanism. This also implies that historical assumptions about the "adsorbed" state of carbon associated with fine fractions need to be re-evaluated. Finally, this work demonstrates that establishing a comprehensive picture of whole soil OM dynamics requires a combination of both methodologies and we offer a suggestion for an efficient combination of the density and size-based approaches.


2012 ◽  
Vol 9 (7) ◽  
pp. 8405-8447 ◽  
Author(s):  
C. Moni ◽  
D. Derrien ◽  
P.-J. Hatton ◽  
B. Zeller ◽  
M. Kleber

Abstract. Physical fractionation is a widely used methodology to study soil organic matter (SOM) dynamics, but concerns have been raised that the available fractionation methods do not well describe functional SOM pools. We also examine the question whether physical fractionation techniques isolate ecologically meaningful, functionally relevant soil compartments. In this study we explore whether the kind of information that aggregate density fractionation (ADF) and particle size-density fractionation (PSDF) yield on soil OM dynamics is method-specific, similar, or complimentary. We do so by following the incorporation of a 15N label into mineral soils of two European beech forests a decade after its application as 15N labelled litter. Both density and size-based fractionation methods suggested that OM became increasingly associated with the mineral phase as decomposition progressed, within aggregates and onto mineral surfaces. Our results suggest that physical fractionation methods do isolate ecologically relevant functional soil subunits. However, scientists investigating specific aspects of OM dynamics are pointed towards ADF when adsorption and aggregation processes are of interest, whereas PSDF is the superior tool to research the fate of particulate organic matter (POM). Some methodological caveats were observed mainly for the PSDF procedure, the most important one being that fine fractions isolated after sonication can not be linked to any defined decomposition pathway or stabilisation process. This also implies that historical assumptions about the "adsorbed" state of carbon associated with fine fractions need to be re-evaluated. Finally, this work demonstrates that establishing a comprehensive picture of whole soil OM dynamics requires a combination of both methodologies and we offer a suggestion for an efficient combination of the density and size-based approaches.


2008 ◽  
Vol 6 (2) ◽  
pp. 52-57
Author(s):  
PRABOWO EDY DAMASTO ◽  
SUDIYONO SUDIYONO ◽  
Y.B.P. SUBAGYO

Damasto PE, Sudiyono, Subagyo YBP. 2008. Influence of temulawak (Curcuma xanthorrhiza) flour addition in ration to digestibility of dry matter and organic matter on male local lamb. Biofarmasi 6): 52-57. Lamb livestock is one of supplier from animal protein in Indonesia, which potential to be developed, but the management system used was still traditional. One of important factor in lamb livestock productivity is feed, therefore the fulfillment of feed, either from its quantity and quality, is absolutely necessary. Besides that, to increase consumption, growth, digestibility, health, and feed efficiency, the livestock needs the existence of feed additive. Feed additive is a special substance that intending to be enhanced in livestock ration for its certain purpose. In this research, feed additive substance was temulawak (Curcuma Xanthorrhiza Roxb) flour. The purpose of this research was to find out the influence of temulawak flour addition in ration to the digestibility of dry matter and organic matter on male local lamb. This research had been done from September 13th, 2007 until November 23rd, 2007 on Minifarm of Animal Husbandry Program of Agriculture Faculty, Sebelas Maret University, which located in Jatikuwung, Gondangrejo, Karanganyar, Central Java. The research used a Completely Randomized Design (CRD). This research used 16 lambs with 12.07±1.11 kg of body weight, divided into four treatments, i.e. P0, P1, P2, and P3, each treatment was consisted of four replications, and each replication used one male local lamb. The treatments were P0 = 0% temulawak flour (as a control), P1 = 0.5% temulawak flour, P2 = 1% temulawak flour, and P3 = 1.5% temulawak flour. The parameters observed were dry matter intake, organic matter intake, dry matter digestibility, and organic matter digestibility. The result of this research for each treatment (P0, P1, P2, P3) on dry matter intake was 681.97, 667.48, 695.72, and 688.04 grams/lamb/day, on organic matter intake were 589.91, 576.64, 600.76, and 595.68 grams/lamb/day, on dry matter digestibility were 64.76, 63.20, 68.27, and 68.39%, and on organic matter digestibility were 70.63, 68.75, 73.15, and 73.44%. Analysis variance showed the different result was not significant at all of parameters and treatments. The conclusion was the addition of temulawak flour until the level of 1.5% from total ration had no effect on dry matter intake, organic matter intake, and dry matter and organic matter digestibility on male local lamb.


2020 ◽  
Vol 24 (2) ◽  
pp. 147
Author(s):  
Alvina Clara Giovanni ◽  
Sedyo Hartono ◽  
Sri Sulandari ◽  
Susamto Somowiyarjo

Begomovirus was reported as one of the most aggressive and destructive viruses on several commercial crops, including cucurbits in Indonesia. Plants that infected with Begomovirus show the mosaic symptom on the leaves, change in leaf shape, stunts, change in color and shape of fruit. It was recently observed in cultivated angled luffa [Luffa acutangula (L.) Roxb] around Yogyakarta and Central Java. The aim of this research was to identify the virus by using Polymerase chain reaction (PCR). The result of Begomovirus amplification from the total DNA samples amplification using primer Krusty-Homer showed that DNA of Begomovirus from angled luffa was amplified at ~580bp. The DNA sequencing of angled luffa’s leaf isolate GD1 had 97.8% homology with SCLV-China isolate MC1. However, amplification of DNA seed samples using the same primer showed negative result. It was concluded that Begomovirus was not a seed borne virus. This is the first molecular report on the occurence of Begomovirus in angled luffa in Yogyakarta.


2021 ◽  
Vol 10 (3) ◽  
pp. 291-298
Author(s):  
Jusup Suprijanto ◽  
Juwita Lesly Senduk ◽  
Dewi Basthika Makrima

Mikroplastik adalah salah satu pencemar laut. Mikroplastik tidak dapat terdegradasi dengan cepat. Mikroplastik merupakan partikel plastik yang berukuran <5mm. Ukurannya yang kecil menyebabkannya dapat tertransportasikan ke seluruh tempat termasuk sistem tubuh ikan dan cumi-cumi. Penelitian dilakukan bertujuan mengetahui mikroplastik pada cumi – cumi (Loligo sp.) dan ikan kembung (Rastrelliger sp.). Penelitian dilakukan bulan Juli 2020 – November 2020 di Semarang Jawa Tengah. Pengambilan sampel dilakukan di TPI Tambak Lorok Semarang.  Pengukuran cumi-cumi dilakukan menggunakan jangka sorong dan ikan diukur menggunakan penggaris. Pembedahan cumi-cumi dan ikan dilakukan menggunakan gunting bedah untuk memisahkan alat pencernaan, tentakel serta insang ikan yang akan digunakan sebagai sampel. Pelarutan dilakukan menggunakan perendaman KOH 10% selama 24 jam untuk melarutkan bahan organik. Pemisahan partikel mikroplastik dilakukan setelah seluruh bahan organik pada sampel larut seluruhnya. Pemisahan partikel mikroplastik dilakukan menggunakan perendaman ZnCl2 selama 24 jam. Penyaringan sampel dilakukan setelah sampel mempunyai 2 layer, layer pertama diambil 10 ml kemudian disaring menggunakan kertas whatman dengan vacump pump. Sampel yang telah disaring dikeringkan, dilanjutkan uji FTIR. Hasil penelitian menunjukkan bahwa terdapat mikroplastik dalam cumi – cumi dan ikan kembung yang ada di TPI Tambak Lorok Semarang, FTIR menunjukkan terdapat polimer plastik jenis nitrile dan nylon. Microplastic is one of the pollutants of the sea. Microplastics do not degrade quickly. Microplastics are plastic particles <5mm in size. Its small size allows it to be transported all over the place including the body systems of fish and squid. The research was conducted to determine the microplastics in squid (Loligo sp.) And mackerel fish (Rastrelliger sp.). The research was conducted in July 2020 - November 2020 in Semarang, Central Java. Sampling was carried out at TPI Tambak Lorok Semarang. Measurements of squid were carried out using a caliper and fish were measured using a ruler. Squid and fish surgery is performed using surgical scissors to separate the digestive organs, tentacles and fish gills that will be used as samples. Dissolving was carried out using 10% KOH immersion for 24 hours to dissolve organic matter. The separation of microplastic particles is carried out after all the organic material in the sample is completely dissolved. The separation of microplastic particles was carried out using ZnCl2 immersion for 24 hours. Filtering of the sample is carried out after the sample has 2 layers, 10 ml of the first layer is taken then filtered using Whatman paper with a vacump pump. The filtered sample was dried, followed by the FTIR test. The results showed that there were microplastics in the squid and mackerel in Tambak Lorok Semarang, FTIR showed that there were plastic polymers of nitrile and nylon types. 


Carbon ◽  
2016 ◽  
Vol 101 ◽  
pp. 361-367 ◽  
Author(s):  
Yun Liu ◽  
Nicola Ferralis ◽  
L. Taras Bryndzia ◽  
Jeffrey C. Grossman

2020 ◽  
Vol 2 (1) ◽  
pp. 64-73
Author(s):  
Eny Fuskhah

The problem experienced by farmer groups in Sumberejo Village, Mranggen Subdistrict, Demak Regency was that it had not managed well the manure that exists to mount beside the cage, the difficulty of providing forage / grass especially in the dry season and the length of time counting grass for livestock, so farmers often provide just the grass or straw he got. Even if the grass was enumerated before being given livestock, it would increase the digestibility so that it would add value to the livestock. The solutions offered were education and counseling for the processing of livestock waste into compost, efforts to utilize rice and corn straw with fermentation technology, and efforts to improve cattle feed by using grass chopping tools.


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