scholarly journals Characteristics of anti-Vibrio harveyi compounds produced by Bacillus spp. isolated from shrimp ponds

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
Iman Rusmana ◽  
ISRAMILDA ISRAMILDA ◽  
Alina Akhdiya
Keyword(s):  
Vibrio Vulnificus ◽  
Vibrio Harveyi ◽  
Pond Water ◽  
Bacillus Sp ◽  
Shrimp Ponds ◽  
Pathogenic Vibrio ◽  
Bacillus Spp ◽  
Ph Range ◽  
Vibrio Spp ◽  
Filtration Technique

Abstract. Rusmana I, Isramilda, Akhdiya A. 2021. Characteristics of anti-Vibrio harveyi compounds produced by Bacillus spp. isolated from shrimp ponds. Biodiversitas 22: 4872-4879. Pathogenic Vibrio spp. such as Vibrio vulnificus, V. alginolyticus, V. fluvialis, V. anguillarum, and V. harveyi caused shrimp diseases. The application of bacterial probiotics can control the growth of pathogenic Vibrio in shrimp. Bacillus spp. can produce antimicrobial compounds that inhibit the growth of pathogenic Vibrio spp. Isolation of Bacillus from several pond water samples, pond sediment, and shrimp intestines was successfully collected 175 isolates of Bacillus spp. Three isolates, i.e., Ltw54, Lts36, dan Lts40 had a high inhibitory index and stability in inhibiting the growth of Vibrio harveyi. Verification assay showed that Bacillus sp. Lts40 had the highest antimicrobial activity. The competition assay showed that Bacillus sp Lts40 isolates inhibited the growth of V. harveyi up to 81,8%. The antimicrobial compound produced by Bacillus sp. Lts40 was stable at the pH range of 3-11 and remained stable after heating at 100ºC for 20 minutes. The purification results using the chromatographic filtration technique showed that the protein fraction with a molecular weight of 47,38 kDa effectively inhibited V. harveyi growth. Bacillus sp. Lts40 isolate has potential application as a probiotic agent in shrimp ponds to control the growth of V. harveyi that causes luminous vibriosis diseases and the antimicrobial substance is potentially to be developed and produced as an anti-V. harveyi product that can be applied in a shrimp hatchery.

scholarly journals Producing Active Secondary Metabolite Against Pathogenic Vibrio spp. by Actinobacteria-Sodium Alginate Co-Culture

2021 ◽  
Vol 26 (4) ◽  
pp. 254-264
Author(s):  
Nuril Azhar ◽  
Ervia Yudiati ◽  
Subagiyo Subagiyo ◽  
Rabia Alghazeer
Keyword(s):  
Secondary Metabolite ◽  
Vibrio Vulnificus ◽  
Bacterial Pathogen ◽  
Disease Outbreaks ◽  
Inhibition Zone ◽  
Marine Biota ◽  
Genus Streptomyces ◽  
Streptomyces Spp ◽  
Pathogenic Vibrio ◽  
Vibrio Spp

Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio harveyi have been found in aquatic environments and suspected as the primary trigger of WFD (White Feces Disease) outbreaks in aquaculture. This Vibrio spp. has an antibiotic resistance to Ampicillin, Co-Amoxiclav, Amoxicillin, Azithromycin, Actinobacteria and Ciprofloxacin HCL. Actinobacteria and alginate have been reported to increase the marine biota resistance against diseases through prebiotic and probiotic mechanisms. This study aims to discover and increase the secondary metabolite production of Actinobacteria-Alginate and its ability as anti-vibrio. Alginate extraction in the samples dated September 2020 originally from Teluk Awur Bays, Jepara, Central Java, Indonesia (33.73±1.84%) was considerably higher than in May 2021 (22.67±0.3%). Samples were taken from sediment and mangrove root. Actinobacteria strains are macroscopically and microscopically similar to the genus Streptomyces. The most well-known antibiotics were produced by Streptomyces spp. The anti-vibrio test was carried out by Kirby-Bauer disc diffusion. The results were observed by measuring the inhibition zone surrounding the paper disc using a digital calliper. Co-culture strain 90 together with alginate have an approved antibacterial activity against all Vibrio spp. in the concentration of 10.disc-1 mg and 5 mg.disc-1. Co-culture Actinobacteria with alginate has remarkably changed the green-yellow color to olive green/dark red-orange (strains 3, 62, 63, 72, and 90), indicating the transformation of the formation alginate with pigments into other compounds through the biosynthetic pathway. Therefore, alginate enables to support of Actinobacteria by induction the active secondary metabolite as an anti-vibrio to counteract the bacterial pathogen diseases.

Multiplex PCR assay for identification of three major pathogenic Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus

2011 ◽  
Vol 25 (4) ◽  
pp. 174-176 ◽  
Author(s):  
Hidemasa Izumiya ◽  
Kazutoshi Matsumoto ◽  
Shunsuke Yahiro ◽  
Jiyoung Lee ◽  
Masatomo Morita ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Cholerae ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Pathogenic Vibrio ◽  
Vibrio Spp

scholarly journals Antibiotic Resistance Analysis of Vibrio spp Isolated from Different Types of Water Sources of Bangladesh and Their Characterization

2019 ◽  
pp. 19-29 ◽  
Keyword(s):  
Antibiotic Resistance ◽  
Vibrio Cholerae ◽  
Diarrheal Disease ◽  
Vibrio Vulnificus ◽  
Culture Media ◽  
Current Knowledge ◽  
Water Sources ◽  
Biomedical Sciences ◽  
Pathogenic Vibrio ◽  
Vibrio Spp

Cholera is an acute form of the diarrheal disease that plagued human civilization over the centuries. The enormity of human sufferings led clinicians and scientists to carry out extensive research on cholera and Vibrio cholerae leading to major discoveries that opened up novel areas of research or new disciplines in biomedical sciences. An attempt is made here to summarize some of these breakthroughs and outline their significance in broader perspectives. In the present study, a total of 12 samples were collected from four types of water sources for isolation of Vibrio spp. Water samples are enrichment into alkaline peptone water then inoculate into culture media such as Nutrient agar, MacConkey, and Thiosulfate Citrate Bile Salts Sucrose (TCBS) agar medium. After incubation of TCBS plates for 24h at 37°C yellow and green colonies are screened out for biochemical identification. No-sucrose fermenting Vibrio parahaemolyticus, Vibrio mimicus, and Vibrio vulnificus show green colonies and sucrose fermenting Vibrio cholerae and Vibrio alginolyticus show yellow colonies on TCBS. To identify the Vibrio spp isolates biochemical test was carried out and typical Vibrio spp give a positive result. The majority of Vibrio spp are avirulent, but certain strains may be sporadically human pathogenic. The antibiotic resistance studies showed that among the 9 isolates were resistant against Erythromycin, Penicillin, Cephalexin, Vancomycin and 4 isolates resistant against Tetracycline. To expand current knowledge of the occurrence, ecological niche and persistence of potential human pathogenic Vibrio spp in aquatic environments, occurrence, and laboratory studies were performed.

Effectiveness of Icing as a Postharvest Treatment for Control of Vibrio vulnificus and Vibrio parahaemolyticus in the Eastern Oyster (Crassostrea virginica)

2008 ◽  
Vol 71 (7) ◽  
pp. 1475-1480 ◽  
Author(s):  
KEVIN MELODY ◽  
RESHANI SENEVIRATHNE ◽  
MARLENE JANES ◽  
LEE ANN JAYKUS ◽  
JOHN SUPAN
Keyword(s):  
Crassostrea Virginica ◽  
Cold Storage ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Storage Period ◽  
Eastern Oyster ◽  
Pathogenic Vibrio ◽  
Postharvest Treatment ◽  
Shellfish Sanitation ◽  
Vibrio Spp

The focus of this research was to investigate the efficacy of icing as a postharvest treatment for reduction of the levels of Vibrio vulnificus and Vibrio parahaemolyticus in commercial quantities of shellstock oysters. The experiments were conducted in June and August of 2006 and consisted of the following treatments: (i) on-board icing immediately after harvest; (ii) dockside icing approximately 1 to 2 h prior to shipment; and (iii) no icing (control). Changes in the levels of pathogenic Vibrio spp. during wholesale and retail handling for 2 weeks postharvest were also monitored. On-board icing achieved temperature reductions in all sacks in accordance with the National Shellfish Sanitation Program standard, but dockside icing did not meet this standard. Based on one-way analysis of variance, the only statistically significant relationship between Vibrio levels and treatment occurred for samples harvested in August; in this case, the levels of V. vulnificus in the noniced oysters were significantly higher (P < 0.05) than were the levels in the samples iced on-board. When analyzing counts over the 14-day storage period, using factorial analysis, there were statistically significant differences in V. vulnificus and V. parahaemolyticus levels by sample date and/or treatment (P < 0.05), but these relationships were not consistent. Treated (iced) oysters had significantly higher gaping (approximately 20%) after 1 week in cold storage than did noniced oysters (approximately 10%) and gaping increased significantly by day 14 of commercial storage. On-board and dockside icing did not predictably reduce the levels of V. vulnificus or V. parahaemolyticus in oysters, and icing negatively impacted oyster survival during subsequent cold storage.

Production and Properties of a Bacterial Thermostable Exo-inulinase

2001 ◽  
Vol 56 (11-12) ◽  
pp. 1022-1028 ◽  
Author(s):  
Kristina Uzunova ◽  
Anna Vassileva ◽  
Margarita Kambourova ◽  
Viara Ivanova ◽  
Dimitrina Spasova ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Enzyme Preparation ◽  
Batch Cultivation ◽  
Crude Enzyme ◽  
Growth Time ◽  
Bacillus Sp ◽  
High Thermostability ◽  
Crude Enzyme Preparation ◽  
Ph Range

Abstract Enzyme production of newly isolated thermophilic inulin-degrading Bacillus sp. 11 strain was studied by batch cultivation in a fermentor. The achieved inulinase and invertase activi­ ties after a short growth time (4.25 h) were similar or higher compared to those reported for other mesophilic aerobic or anaerobic thermophilic bacterial producers and yeasts. The investigated enzyme belonged to the exo-type inulinases and splitted-off inulin, sucrose and raffinose. It could be used at temperatures above 65 °C and pH range 5.5-7.5. The obtained crude enzyme preparation possessed high thermostability. The residual inulinase and inver­ tase activities were 92-98% after pretreatment at 65 °C for 60 min in the presence of substrate inulin.

scholarly journals Regulation of Metalloprotease Gene Expression in Vibrio vulnificus by a Vibrio harveyi LuxR Homologue

2001 ◽  
Vol 183 (4) ◽  
pp. 1369-1375 ◽  
Author(s):  
Chung-Ping Shao ◽  
Lien-I Hor
Keyword(s):  
Parent Strain ◽  
Vibrio Vulnificus ◽  
Vibrio Harveyi ◽  
Negative Regulation ◽  
Open Reading Frame ◽  
Protease Gene ◽  
Reading Frame ◽  
Allelic Exchange ◽  
Exchange Technique

ABSTRACT Expression of the Vibrio vulnificus metalloprotease gene, vvp, was turned up rapidly when bacterial growth reached the late log phase. A similar pattern of expression has been found in the metalloprotease gene of Vibrio cholerae, and this has been shown to be regulated by a Vibrio harveyiLuxR-like transcriptional activator. To find out whether a LuxR homologue exists in V. vulnificus, a gene library of this organism was screened by colony hybridization using a probe derived from a sequence that is conserved in various luxR-like genes of vibrios. A gene containing a 618-bp open reading frame was identified and found to be identical to the smcR gene ofV. vulnificus reported previously. An isogenic SmcR-deficient (RD) mutant was further constructed by an in vivo allelic exchange technique. This mutant exhibited an extremely low level of vvp transcription compared with that of the parent strain. On the other hand, the cytolysin gene, vvhA, was expressed at a higher level in the RD mutant than in the parent strain during the log phase of growth. These data suggested that SmcR might not only be a positive regulator of the protease gene but might also be involved in negative regulation of the cytolysin gene. Virulence of the RD mutant in either normal or iron-overloaded mice challenged by intraperitoneal injection was comparable to that of the parent strain, indicating that SmcR is not required for V. vulnificusvirulence in mice.

scholarly journals Complete Genome Sequence of the Pathogenic Vibrio vulnificus Type Strain ATCC 27562

2017 ◽  
Vol 5 (35) ◽  
Author(s):  
Douglas B. Rusch ◽  
Dean A. Rowe-Magnus
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Type Strain ◽  
Vibrio Vulnificus ◽  
Foodborne Pathogen ◽  
The United States ◽  
Environmental Isolates ◽  
Content Type ◽  
Pathogenic Vibrio

ABSTRACT Vibrio vulnificus has the highest death rate and economic burden per case of any foodborne pathogen in the United States. A complete genome sequence of the type strain promotes comparative analyses with other clinical and environmental isolates, improving our understanding of this important human pathogen and successful environmental organism.

The Effect of Low-Rate Sand Filtration and Modified Feed Management on Effluent Quality, Pond Water Quality and Production of Intensive Shrimp Ponds

Estuaries ◽  
1995 ◽  
Vol 18 (1) ◽  
pp. 116 ◽  
Author(s):  
J. Stephen Hopkins ◽  
Craig L. Browdy ◽  
Richard D. Hamilton ◽  
Joseph A. Heffernan
Keyword(s):  
Water Quality ◽  
Pond Water ◽  
Sand Filtration ◽  
Shrimp Ponds ◽  
Effluent Quality ◽  
Feed Management ◽  
Low Rate

scholarly journals Isolation and selection of bacteria against shrimp pathogenic Vibrio parahaemolyticus from shrimp pond water on Duyen Hai district, Tra Vinh province

2020 ◽  
Vol 10 (1) ◽  
pp. 43-52
Author(s):  
Tran Vu Phuong ◽  
Dang Thi Ngoc Thanh ◽  
Cao Ngoc Diep
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Pathogenic Bacteria ◽  
Pond Water ◽  
Shrimp Farming ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Shrimp Pond ◽  
Pathogenic Vibrio ◽  
The Status

Antibiotic has frequently been used in the shrimp-farming process in Vietnam. This leads to the status that antibiotic-resistant bacteria and products do not receive in the market. Bacteria had the resistant ability to pathogenic bacteria in water, and they have an important role in sustainable aquaculture. This study aimed to isolate and select good bacterial strains against Vibrio parahaemolyticus, pathogenic bacteria, on shrimp from 8 samples of shrimp pond water at 3 villages Ngu Lac, Phuoc An and Long Toan of Duyen Hai district, Tra Vinh province on NB agar medium. As a result, fifty-nine bacterial isolates were isolated and 10/59 isolates (16.95%) were identified as resistant to Vibrio parahaemolyticus by the well diffusion method. In 10 isolates, there were 7 isolates had good resistance to select for PCR technique and sequencing. The result indicated that these seven strains, including DH1m, DH2f, DH4d, DH8i, DH8m, DH8n, belonged to Bacilli and DH1n strain belonged to Streptomyces sp.

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