Characterization of Muscle Spindle Afferents in the Adult Mouse Using an In Vitro Muscle-Nerve Preparation

Katherine A. Wilkinson; Heidi E. Kloefkorn; Shawn Hochman

doi:10.1371/journal.pone.0039140

Characterization of Muscle Spindle Afferents in the Adult Mouse Using an In Vitro Muscle-Nerve Preparation

PLoS ONE ◽

10.1371/journal.pone.0039140 ◽

2012 ◽

Vol 7 (6) ◽

pp. e39140 ◽

Cited By ~ 15

Author(s):

Katherine A. Wilkinson ◽

Heidi E. Kloefkorn ◽

Shawn Hochman

Keyword(s):

Muscle Spindle ◽

Adult Mouse ◽

Muscle Spindle Afferents ◽

Muscle Nerve

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Characterization of adult mouse muscle spindle afferents in an in vitro isolated muscle nerve preparation

The FASEB Journal ◽

10.1096/fasebj.25.1_supplement.1051.29 ◽

2011 ◽

Vol 25 (S1) ◽

Author(s):

Katherine Wilkinson ◽

Heidi Kloefkorn ◽

Shawn Hochman

Keyword(s):

Muscle Spindle ◽

Adult Mouse ◽

Muscle Spindle Afferents ◽

Mouse Muscle ◽

Muscle Nerve

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Regeneration and characterization of adult mouse hippocampal neurons in a defined in vitro system

Journal of Neuroscience Methods ◽

10.1016/j.jneumeth.2008.09.022 ◽

2009 ◽

Vol 177 (1) ◽

pp. 51-59 ◽

Cited By ~ 11

Author(s):

Kucku Varghese ◽

Mainak Das ◽

Neelima Bhargava ◽

Maria Stancescu ◽

Peter Molnar ◽

...

Keyword(s):

Hippocampal Neurons ◽

Adult Mouse ◽

In Vitro System

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An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents

Journal of Visualized Experiments ◽

10.3791/51948 ◽

2014 ◽

Cited By ~ 2

Author(s):

Joy A. Franco ◽

Heidi E. Kloefkorn ◽

Shawn Hochman ◽

Katherine A. Wilkinson

Keyword(s):

Adult Mouse ◽

Muscle Afferents ◽

Mouse Muscle ◽

Muscle Nerve ◽

Firing Properties

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In vitro characterization of neural stem cells: Functional response to the enteric neurotrophin GDNF and co-culture with human colonic smooth muscle

Gastroenterology ◽

10.1016/s0016-5085(01)80307-3 ◽

2001 ◽

Vol 120 (5) ◽

pp. A62-A62

Author(s):

M MICCI ◽

R LEARISH ◽

P PASRICHA

Keyword(s):

Stem Cells ◽

Smooth Muscle ◽

Neural Stem Cells ◽

Functional Response ◽

In Vitro Characterization

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Biological and physico-chemical characterization of ultra high diluted Hydrastis canadensis and in vitro studies on mammalian cells

Allgemeine Homöopathische Zeitung ◽

10.1055/s-0037-1601241 ◽

2017 ◽

Vol 262 (02) ◽

pp. 2-76

Author(s):

N Chandrakar ◽

MK Temgire ◽

SG Kane ◽

AK Suresh ◽

J Bellare

Keyword(s):

Mammalian Cells ◽

Chemical Characterization ◽

In Vitro Studies ◽

Hydrastis Canadensis ◽

Physico Chemical

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Assays for Phospholipid-Dependent Formation of Thrombin and Xa: A Potential Method for Quantifying Lupus Anticoagulant Activity

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646437 ◽

1991 ◽

Vol 66 (04) ◽

pp. 453-458 ◽

Cited By ~ 15

Author(s):

John T Brandt

Keyword(s):

Specific Activity ◽

Anticoagulant Activity ◽

Factor Xa ◽

Clinical Importance ◽

Potential Method ◽

Quantitative Expression ◽

Increased Risk ◽

Apparent Association

SummaryLupus anticoagulants (LAs) are antibodies which interfere with phospholipid-dependent procoagulant reactions. Their clinical importance is due to their apparent association with an increased risk of thrombo-embolic disease. To date there have been few assays for quantifying the specific activity of these antibodies in vitro and this has hampered attempts to purify and characterize these antibodies. Methods for determining phospholipid-dependent generation of thrombin and factor Xa are described. Isolated IgG fractions from 7 of 9 patients with LAs were found to reproducibly inhibit enzyme generation in these assay systems, permitting quantitative expression of inhibitor activity. Different patterns of inhibitory activity, based on the relative inhibition of thrombin and factor Xa generation, were found, further substantiating the known heterogeneity of these antibodies. These systems may prove helpful in further purification and characterization of LAs.

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Characterization of the Original Christmas Disease Mutation (Cysteine 206 → Serine): From Clinical Recognition to Molecular Pathogenesis

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648381 ◽

1992 ◽

Vol 67 (01) ◽

pp. 063-065 ◽

Cited By ~ 6

Author(s):

Sherryl A M Taylor ◽

Jacalyn Duffin ◽

Cherie Cameron ◽

Jerome Teitel ◽

Bernadette Garvey ◽

...

Keyword(s):

Nucleotide Substitution ◽

Novel Mutation ◽

Factor Ix ◽

Causative Mutation ◽

Coding Region ◽

Body Of Knowledge ◽

Polymerase Chain ◽

Splice Junctions

SummaryChristmas disease was first reported as a distinct clinical entity in two manuscripts published in 1952 (1, 2). The eponym associated with this disorder, is the surname of the first patient examined in detail and reported by Biggs and colleagues in a paper describing the clinical and laboratory features of seven affected individuals (3). This patient has severe factor IX coagulant deficiency (less than 0.01 units/ml) and no detectable circulating factor IX antigen (less than 0.01 units/ml). Coding sequence and splice junctions of the factor IX gene from this patient have been amplified in vitro through the polymerase chain reaction (PCR). One nucleotide substitution was identified at nucleotide 30,070 where a guanine was replaced by a cytosine. This mutation alters the amino acid encoded at position 206 in the factor IX protein from cysteine to serine. The non conservative nature of this substitution, the absence of this change in more than 200 previously sequenced factor IX genes and the fact that the remainder of the coding region of this gene was normal, all provide strong circumstantial evidence in favour of this change being the causative mutation in this patient. The molecular characterization of this novel mutation in the index case of Christmas disease, contributes to the rapidly expanding body of knowledge pertaining to Christmas disease pathogenesis.

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