scholarly journals Acroframosome-Dependent KIFC1 Facilitates Acrosome Formation during Spermatogenesis in the Caridean Shrimp Exopalaemon modestus

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e76065 ◽  
Author(s):  
Cong-Cong Hou ◽  
Wan-Xi Yang
Keyword(s):  
Caridean Shrimp ◽  
Exopalaemon Modestus ◽  
Acrosome Formation

Antiviral activity of cathelicidin 5, a peptide from Alligator sinensis, against WSSV in caridean shrimp Exopalaemon modestus

2019 ◽  
Vol 93 ◽  
pp. 82-89
Author(s):  
Qing Xie ◽  
Yang Liu ◽  
Fangmei Luo ◽  
Qingqing Yi ◽  
Yipeng Wang ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Caridean Shrimp ◽  
Exopalaemon Modestus ◽  
Alligator Sinensis

scholarly journals Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 80
Author(s):  
Xin Li ◽  
Chenying Duan ◽  
Ruyi Li ◽  
Dong Wang
Keyword(s):  
Gene Expression ◽  
Semen Quality ◽  
Regulation Of Gene Expression ◽  
Physiological Mechanism ◽  
Interaction Network ◽  
Seminiferous Tubules ◽  
Differential Analysis ◽  
Epididymal Sperm ◽  
Bioinformatics Analyses ◽  
Acrosome Formation

To reduce subfertility caused by low semen quality and provide theoretical guidance for the eradication of human male infertility, we sequenced the bovine transcriptomes of round, elongated spermatids and epididymal sperms. The differential analysis was carried out with the reference of the mouse transcriptome, and the homology trends of gene expression to the mouse were also analysed. First, to explore the physiological mechanism of spermiogenesis that profoundly affects semen quality, homological trends of differential genes were compared during spermiogenesis in dairy cattle and mice. Next, Gene Ontology (GO), Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment, protein–protein interaction network (PPI network), and bioinformatics analyses were performed to uncover the regulation network of acrosome formation during the transition from round to elongated spermatids. In addition, processes that regulate gene expression during spermiogenesis from elongated spermatid to epididymal sperm, such as ubiquitination, acetylation, deacetylation, and glycosylation, and the functional ART3 gene may play important roles during spermiogenesis. Therefore, its localisation in the seminiferous tubules and epididymal sperm were investigated using immunofluorescent analysis, and its structure and function were also predicted. Our findings provide a deeper understanding of the process of spermiogenesis, which involves acrosome formation, histone replacement, and the fine regulation of gene expression.

Effect of Stocking Size on Growth Performance, Biomass, Production, Yield, and Survival of Caridean Shrimp Cage-Cultured in a Pond System

2014 ◽  
Vol 76 (4) ◽  
pp. 430-435 ◽  
Author(s):  
Jesús T. Ponce-Palafox ◽  
Fermín López-Uriostegui ◽  
José L. Arredondo-Figueroa ◽  
Mario A. Benítez-Mandujano ◽  
Manuel García-Ulloa Gómez ◽  
...  
Keyword(s):  
Growth Performance ◽  
Biomass Production ◽  
Production Yield ◽  
Caridean Shrimp ◽  
Pond System

scholarly journals Loss of Profilin3 Impairs Spermiogenesis by Affecting Acrosome Biogenesis, Autophagy, Manchette Development and Mitochondrial Organization

2021 ◽  
Vol 9 ◽  
Author(s):  
Naila Umer ◽  
Lena Arévalo ◽  
Sharang Phadke ◽  
Keerthika Lohanadan ◽  
Gregor Kirfel ◽  
...  
Keyword(s):  
Actin Polymerization ◽  
Regulatory Proteins ◽  
Autophagic Flux ◽  
Nuclear Fraction ◽  
Rna Seq ◽  
Head Shape ◽  
Male Factor ◽  
Protein Levels ◽  
Acrosome Formation ◽  
Actin Regulatory Proteins

Profilins (PFNs) are key regulatory proteins for the actin polymerization in cells and are encoded in mouse and humans by four Pfn genes. PFNs are involved in cell mobility, cell growth, neurogenesis, and metastasis of tumor cells. The testes-specific PFN3 is localized in the acroplaxome–manchette complex of developing spermatozoa. We demonstrate that PFN3 further localizes in the Golgi complex and proacrosomal vesicles during spermiogenesis, suggesting a role in vesicle transport for acrosome formation. Using CRISPR/Cas9 genome editing, we generated mice deficient for Pfn3. Pfn3–/– males are subfertile, displaying a type II globozoospermia. We revealed that Pfn3–/– sperm display abnormal manchette development leading to an amorphous sperm head shape. Additionally, Pfn3–/– sperm showed reduced sperm motility resulting from flagellum deformities. We show that acrosome biogenesis is impaired starting from the Golgi phase, and mature sperm seems to suffer from a cytoplasm removal defect. An RNA-seq analysis revealed an upregulation of Trim27 and downregulation of Atg2a. As a consequence, mTOR was activated and AMPK was suppressed, resulting in the inhibition of autophagy. This dysregulation of AMPK/mTOR affected the autophagic flux, which is hallmarked by LC3B accumulation and increased SQSTM1 protein levels. Autophagy is involved in proacrosomal vesicle fusion and transport to form the acrosome. We conclude that this disruption leads to the observed malformation of the acrosome. TRIM27 is associated with PFN3 as determined by co-immunoprecipitation from testis extracts. Further, actin-related protein ARPM1 was absent in the nuclear fraction of Pfn3–/– testes and sperm. This suggests that lack of PFN3 leads to destabilization of the PFN3–ARPM1 complex, resulting in the degradation of ARPM1. Interestingly, in the Pfn3–/– testes, we detected increased protein levels of essential actin regulatory proteins, cofilin-1 (CFL1), cofilin-2 (CFL2), and actin depolymerizing factor (ADF). Taken together, our results reveal the importance for PFN3 in male fertility and implicate this protein as a candidate for male factor infertility in humans.

scholarly journals Hypomorphic and hypermorphic mouse models of Fsip2 indicate its dosage-dependent roles in sperm tail and acrosome formation

Development ◽  
2021 ◽  
Vol 148 (11) ◽  
Author(s):  
Xiang Fang ◽  
Yaser Gamallat ◽  
Zhiheng Chen ◽  
Hanran Mai ◽  
Pei Zhou ◽  
...  
Keyword(s):  
Mouse Model ◽  
Physical Interaction ◽  
Loss Of Function ◽  
Internal Fertilization ◽  
Fibrous Sheath ◽  
Altered Expression ◽  
Sperm Tail ◽  
Acrosomal Vesicle ◽  
Truncating Mutation ◽  
Acrosome Formation

ABSTRACT Loss-of-function mutations in multiple morphological abnormalities of the sperm flagella (MMAF)-associated genes lead to decreased sperm motility and impaired male fertility. As an MMAF gene, the function of fibrous sheath-interacting protein 2 (FSIP2) remains largely unknown. In this work, we identified a homozygous truncating mutation of FSIP2 in an infertile patient. Accordingly, we constructed a knock-in (KI) mouse model with this mutation. In parallel, we established an Fsip2 overexpression (OE) mouse model. Remarkably, KI mice presented with the typical MMAF phenotype, whereas OE mice showed no gross anomaly except for sperm tails with increased length. Single-cell RNA sequencing of the testes uncovered altered expression of genes related to sperm flagellum, acrosomal vesicle and spermatid development. We confirmed the expression of Fsip2 at the acrosome and the physical interaction of this gene with Acrv1, an acrosomal marker. Proteomic analysis of the testes revealed changes in proteins sited at the fibrous sheath, mitochondrial sheath and acrosomal vesicle. We also pinpointed the crucial motifs of Fsip2 that are evolutionarily conserved in species with internal fertilization. Thus, this work reveals the dosage-dependent roles of Fsip2 in sperm tail and acrosome formation.

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