scholarly journals Generation and Analysis of a Large-Scale Expressed Sequence Tag Database from a Full-Length Enriched cDNA Library of Developing Leaves of Gossypium hirsutum L

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e76443 ◽  
Author(s):  
Min Lin ◽  
Deyong Lai ◽  
Chaoyou Pang ◽  
Shuli Fan ◽  
Meizhen Song ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Cdna Library ◽  
Large Scale ◽  
Expressed Sequence Tag ◽  
Full Length ◽  
Gossypium Hirsutum L ◽  
Expressed Sequence

Construction of a Full-Length cDNA Library of Gossypium hirsutum L. and Identification of Two MADS-Box Genes

2011 ◽  
Vol 10 (1) ◽  
pp. 28-40 ◽  
Author(s):  
Li-na WANG ◽  
Dong WU ◽  
Shu-xun YU ◽  
Shu-li FAN ◽  
Mei-zhen SONG ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Cdna Library ◽  
Full Length ◽  
Mads Box ◽  
Mads Box Genes ◽  
Gossypium Hirsutum L ◽  
Full Length Cdna

An expressed sequence tag analysis of a full-length, spliced-leader cDNA library from Leishmania major promastigotes

1996 ◽  
Vol 76 (1-2) ◽  
pp. 345-348 ◽  
Author(s):  
Mark P. Levick ◽  
Jenefer M. Blackwell ◽  
Vivienne Connor ◽  
Richard M.R. Coulson ◽  
Alistair Miles ◽  
...  
Keyword(s):  
Cdna Library ◽  
Leishmania Major ◽  
Expressed Sequence Tag ◽  
Full Length ◽  
Spliced Leader ◽  
Expressed Sequence Tag Analysis ◽  
Expressed Sequence

scholarly journals A full-length enriched cDNA library and expressed sequence tag analysis of the parasitic weed, Striga hermonthica

2010 ◽  
Vol 10 (1) ◽  
pp. 55 ◽  
Author(s):  
Satoko Yoshida ◽  
Juliane K Ishida ◽  
Nasrein M Kamal ◽  
Abdelbagi M Ali ◽  
Shigetou Namba ◽  
...  
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tag ◽  
Full Length ◽  
Striga Hermonthica ◽  
Parasitic Weed ◽  
Expressed Sequence Tag Analysis ◽  
Expressed Sequence

scholarly journals Generation and analysis of large-scale expressed sequence tags (ESTs) from a full-length enriched cDNA library of porcine backfat tissue

BMC Genomics ◽  
2006 ◽  
Vol 7 (1) ◽  
Author(s):  
Tae-Hun Kim ◽  
Nam-Soon Kim ◽  
Dajeong Lim ◽  
Kyung-Tai Lee ◽  
Jung-Hwa Oh ◽  
...  
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Large Scale ◽  
Full Length ◽  
Expressed Sequence

scholarly journals Analysis of Expressed Sequence Tag Data and Gene Expression Profiles Involved in Conidial Germination of Fusarium oxysporum

2006 ◽  
Vol 72 (2) ◽  
pp. 1667-1671 ◽  
Author(s):  
Ye Deng ◽  
Haitao Dong ◽  
Qingchao Jin ◽  
Cheng'en Dai ◽  
Yongqi Fang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Synthesis ◽  
Fusarium Oxysporum ◽  
Cdna Library ◽  
Expressed Sequence Tag ◽  
Expression Profiles ◽  
Cdna Array ◽  
Gene Expression Profiles ◽  
Conidial Germination ◽  
Expressed Sequence

ABSTRACT We obtained 3,372 tentative unique transcripts (TUTs) from a cDNA library of Fusarium oxysporum. A cDNA array with 3,158 TUTs was produced to analyze gene expression profiles in conidial germination. It seems that ras and other signaling genes, e.g., ccg, cooperatively initiate conidial germination in Fusarium by increasing protein synthesis.

scholarly journals Generation and Analysis of Expressed Sequence Tags (ESTs) from Muscle Full-Length cDNA Library of Wujin Pig

2014 ◽  
Vol 13 (2) ◽  
pp. 378-386
Author(s):  
Su-mei ZHAO ◽  
Yong-gang LIU ◽  
Hong-bing PAN ◽  
Xi ZHANG ◽  
Chang-rong GE ◽  
...  
Keyword(s):  
Cdna Library ◽  
Expressed Sequence Tags ◽  
Full Length ◽  
Full Length Cdna ◽  
Expressed Sequence

scholarly journals Beta-centractin: characterization and distribution of a new member of the centractin family of actin-related proteins.

1994 ◽  
Vol 5 (12) ◽  
pp. 1301-1310 ◽  
Author(s):  
S W Clark ◽  
O Staub ◽  
I B Clark ◽  
E L Holzbaur ◽  
B M Paschal ◽  
...  
Keyword(s):  
Amino Acid ◽  
Cdna Clone ◽  
Expressed Sequence Tag ◽  
Full Length ◽  
Northern Analysis ◽  
Related Protein ◽  
Two Dimensional Gel Electrophoresis ◽  
Full Length Cdna ◽  
Dynactin Complex ◽  
Expressed Sequence

An examination of human-expressed sequence tags indicated the existence of an isoform of centractin, an actin-related protein localized to microtubule-associated structures. Using one of these tags, we isolated and determined the nucleotide sequence of a full-length cDNA clone. The protein encoded represents the first example of multiple isoforms of an actin-related protein in a single organism. Northern analysis using centractin-specific probes revealed three species of mRNA in HeLa cells that could encode centractin isoforms. One mRNA encodes the previously-identified centractin (now referred to as alpha-centractin). The full-length cDNA clone isolated using the expressed sequence tag encodes a new member of the centractin family, beta-centractin. A probe specific for alpha-centractin hybridized to the third species of mRNA observed (referred to as gamma-centractin). Comparisons of Northern blots of human tissues indicated that alpha-centractin and beta-centractin mRNAs are equally distributed in all populations of mRNA examined, whereas the expression of gamma-centractin appears to be tissue specific. The amino acid sequence of beta-centractin, deduced from the cDNA, indicates a 91% identity with alpha-centractin, increasing to 96% similarity when conservative amino acid changes are taken into account. As antibodies previously raised against alpha-centractin reacted only poorly with beta-centractin, new antibodies were produced and combined with two-dimensional gel electrophoresis to discriminate the two isoforms. Using this system, the subcellular distribution of the alpha- and beta-isoforms were determined. Both isoforms were found predominantly in the cytosolic fraction as a part of a previously identified 20S complex (referred to as the dynactin complex) with no evidence for a free pool of either isoform. The isoforms were found in a constant ratio of approximately 15:1 (alpha:beta) in the dynactin complex.

Sign in / Sign up

Export Citation Format

Share Document