scholarly journals Pilot study on the value of Raman spectroscopy in the entity assignment of salivary gland tumors

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257470
Author(s):  
Till Jasper Meyer ◽  
Elena Gerhard-Hartmann ◽  
Nina Lodes ◽  
Agmal Scherzad ◽  
Rudolf Hagen ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Salivary Gland ◽  
Tissue Sample ◽  
Principal Component ◽  
Salivary Gland Tumors ◽  
Proof Of Concept ◽  
Linear Discriminant ◽  
Adenoid Cystic ◽  
Pleomorphic Adenomas ◽  
Chemometric Algorithms

Background The entity assignment of salivary gland tumors (SGT) based on histomorphology can be challenging. Raman spectroscopy has been applied to analyze differences in the molecular composition of tissues. The aim of this study was to evaluate the suitability of RS for entity assignment in SGT. Methods Raman data were collected in deparaffinized sections of pleomorphic adenomas (PA) and adenoid cystic carcinomas (ACC). Multivariate data and chemometric analysis were completed using the Unscrambler software. Results The Raman spectra detected in ACC samples were mostly assigned to nucleic acids, lipids, and amides. In a principal component-based linear discriminant analysis (LDA) 18 of 20 tumor samples were classified correctly. Conclusion In this proof of concept study, we show that a reliable SGT diagnosis based on LDA algorithm appears possible, despite variations in the entity-specific mean spectra. However, a standardized workflow for tissue sample preparation, measurement setup, and chemometric algorithms is essential to get reliable results.

Raman Spectroscopy for Inverted Papilloma: A Proof-of-Concept Study

2018 ◽  
Vol 159 (3) ◽  
pp. 587-589 ◽  
Author(s):  
Marco A. Mascarella ◽  
Abdulaziz Alrasheed ◽  
Naif Fnais ◽  
Ophelie Gourgas ◽  
Ghulam Jalani ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Raman Spectroscopy ◽  
Chronic Rhinosinusitis ◽  
Principal Component ◽  
Inverted Papilloma ◽  
Proof Of Concept ◽  
Linear Discriminant ◽  
Acceptable Accuracy ◽  
Sinonasal Mucosa ◽  
Inverted Papillomas

Inverted papillomas are tumors of the sinonasal tract with a propensity to recur. Raman spectroscopy can potentially identify inverted papillomas from other tissue based on biochemical signatures. A pilot study comparing Raman spectroscopy to histopathology for 3 types of sinonasal tissue was performed. Spectral data of biopsies from patients with normal sinonasal mucosa, chronic rhinosinusitis, and inverted papillomas are compared to histopathology using principal component analysis and linear discriminant analysis after data preprocessing. A total of 18 normal, 15 chronic rhinosinusitis, and 18 inverted papilloma specimens were evaluated. The model distinguished normal sinonasal mucosa, chronic rhinosinusitis, and inverted papilloma tissue with an overall accuracy of 90.2% (95% confidence interval, 0.86-0.94). In conclusion, Raman spectroscopy can distinguish inverted papilloma, normal sinonasal mucosa, and chronically rhinosinusitis tissue with acceptable accuracy.

scholarly journals Immunohistochemical expression of vimentin, calponin and HHF-35 in salivary gland tumors

2007 ◽  
Vol 18 (3) ◽  
pp. 192-197 ◽  
Author(s):  
Roberta Barroso Cavalcante ◽  
Fernanda Ferreira Lopes ◽  
Andréa Soares Ferreira ◽  
Roseana de Almeida Freitas ◽  
Lélia Batista de Souza
Keyword(s):  
Salivary Gland ◽  
Myoepithelial Cells ◽  
Salivary Gland Tumors ◽  
Low Grade ◽  
Tubular Structures ◽  
Immunohistochemical Markers ◽  
Adenoid Cystic ◽  
Peripheral Cells ◽  
Diffuse Distribution ◽  
Labeling Pattern

Myoepithelial cells present a complex immunophenotype, with the expression of proteins varying according to the stage of normal or neoplastic differentiation of the cell. In order to evaluate the immunohistochemical markers expressed by these cells, a panel of antibodies composed of vimentin, calponin and HHF-35 was applied to 28 salivary gland tumors. The results demonstrated a higher percent sensitivity of vimentin and calponin compared to HHF-35. However, calponin and HHF-35 presented a focal labeling pattern in contrast with the diffuse distribution of vimentin. The cells predominantly stained by all tested antibodies included nonluminal cells in duct-like and tubular structures, such as those seen in pleomorphic adenomas and adenoid cystic carcinomas, as well as cells in the cords and nests of polymorphous low-grade adenocarcinomas and peripheral cells of sheets and nests of myoepitheliomas. In conclusion, the combination of calponin and vimentin is suggested for the identification of myoepithelial cells in salivary gland tumors.

scholarly journals Epidemiologic Characteristics of Salivary Gland Tumors in an Iranian Population

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Nafise Shamloo ◽  
Alireza Ghanadan ◽  
Fahimeh Sadat Hashemian ◽  
Maedeh Ghorbanpour
Keyword(s):  
Salivary Gland ◽  
Adenoid Cystic Carcinoma ◽  
Salivary Glands ◽  
Pleomorphic Adenoma ◽  
Malignant Tumors ◽  
The Body ◽  
Salivary Gland Tumors ◽  
Benign Tumors ◽  
Cross Sectional ◽  
Adenoid Cystic

Background: Salivary gland tumors include a wide variety of benign and malignant tumors in the oral and maxillofacial region. Although these tumors are not common, they are not rare. The prevalence of these tumors varies with regard to age, gender, and their location in the body. Objectives: This study aimed to evaluate the frequency of benign and malignant salivary gland tumors in patients referred to three referral hospitals in Tehran, Iran. Methods: This retrospective cross-sectional study examined the demographic and pathologic records of the patients with salivary gland tumors submitted to the Department of Pathology of Amir Alam, Loghman Hakim, and Shohada Hospitals from 2005 to 2016. In this study, the histological variants of salivary gland tumors and clinical parameters such as age, gender, and the location of the tumor were examined. The clinical data were analyzed using SPSS software version 21. Results: Of 137632 patient records, 1180 cases were salivary gland tumors. Pleomorphic adenoma in 794 cases (67.3%) and adenoid cystic carcinoma in 109 cases (9.2%) were the most common tumors, respectively. Salivary gland tumors were more common in males, and the participants’ mean age was 42.86 ± 16.5 years. The most common site was parotid and minor salivary glands, with 937 (79.4%) and 137 (12%) cases, respectively. Conclusions: In this study, the most common benign tumor was pleomorphic adenoma in the parotid gland, and the most common malignant tumor was adenoid cystic carcinoma in the major salivary glands. Furthermore, benign tumors were more frequent than malignant tumors.

The Myoepithelial Immunophenotype in 135 Benign and Malignant Salivary Gland Tumors Other Than Pleomorphic Adenoma

1999 ◽  
Vol 123 (9) ◽  
pp. 801-806 ◽  
Author(s):  
Anil R. Prasad ◽  
Adnan T. Savera ◽  
Allen M. Gown ◽  
Richard J. Zarbo
Keyword(s):  
Smooth Muscle ◽  
Salivary Gland ◽  
Salivary Gland Tumors ◽  
Low Grade ◽  
Salivary Duct ◽  
Not Otherwise Specified ◽  
Adenoid Cystic ◽  
Myoepithelial Differentiation ◽  
Specific Proteins ◽  
Malignant Salivary Gland Tumors

Abstract Background.—We have previously studied the immunoreactivity of 3 novel smooth muscle–specific proteins, α-smooth muscle actin, smooth muscle myosin heavy chains, and calponin, to assess myoepithelial differentiation in pleomorphic adenomas. Objective.—To further expand our knowledge of myoepithelial differentiation in other benign and malignant salivary gland tumors. Design.—Formalin-fixed paraffin sections of 135 salivary gland tumors with associated normal glands were stained with monoclonal antibodies using the avidin-biotin complex immunoperoxidase method and enzymatic and microwave heat–induced epitope retrieval. Results.—In adenoid cystic carcinomas and epithelial-myoepithelial carcinomas, all 3 markers exclusively highlighted the myoepithelial cell components and the epithelial cells were entirely negative. No immunostaining was detected in canalicular adenomas, oncocytomas, Warthin tumors, acinic cell carcinomas, mucoepidermoid carcinomas, squamous cell carcinomas, and polymorphous low-grade adenocarcinomas. Salivary duct carcinomas and adenocarcinomas, not otherwise specified had a distinctive pattern of uniform periductal staining of reactive myofibroblastic cells, and in salivary duct carcinomas some ducts retained a peripheral immunoreactive myoepithelial cell layer. Conclusion.—Immunoreactivity for these 3 smooth muscle–specific proteins confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas. Periductal linear staining in adenocarcinoma, not otherwise specified and salivary duct carcinomas is distinctive and appears to represent a tight cuff of myofibroblasts associated with the infiltrating glands.

High-Risk Human Papillomavirus (HPV) in Parotid Lesions

2007 ◽  
Vol 22 (4) ◽  
pp. 239-244 ◽  
Author(s):  
D. Vageli ◽  
G. Sourvinos ◽  
M. Ioannou ◽  
G.K. Koukoulis ◽  
D.A. Spandidos
Keyword(s):  
Salivary Gland ◽  
Viral Load ◽  
High Risk ◽  
Salivary Gland Tumors ◽  
Specific Primers ◽  
In Situ Pcr ◽  
Hpv Genotypes ◽  
Pleomorphic Adenomas ◽  
High Risk Hpv

Although several studies have reported that oropharyngeal infection with HPV may predispose to tumorigenesis, little is known about the etiological factors of salivary gland tumors and the presence of HPV We studied 9 parotid lesions for HPV infection including an oncocytoma, an acinic cell carcinoma, a high-grade adenocarcinoma, a low-grade polymorphous adenocarcinoma, a Warthin's tumor and 2 pleomorphic adenomas, a lymphoepithelial cyst and a lipoma of the parotid gland. DNA was extracted from formalin-fixed and paraffin-embedded tissue sections. Solution PCR for HPV detection was performed using the GP5+/GP6+ primers, while HPV typing was carried out by multiplex PCR for HPV6, 11, 16, 18, and 33; positive samples were recorfirmed by PCR with specific primers for each type. Quantitative real-time PCR for the high-risk HPV genotypes 16, 18, 31, 33, 35, 52, 58 and 67 was also performed to quantitate the viral load. Finally, in situ PCR was employed with HPV16-specific primers by direct-detection method. Seven of the 9 parotid lesions were HPV positive while 6 of these 7 had been infected by HPV16 and/or HPV18 oncogenic types. High viral load of high-risk genotypes of HPV was found in the oncocytoma, in one of the pleomorphic adenomas, and in the Warthin's tumor. Finally, in situ PCR indicated that HPV16 amplification occurred in the salivary gland tumors. This is the first time that high-risk HPV genotypes are detected in these histological types of parotid lesions, suggesting the possible involvement of the virus in the disease.

Benign Pleomorphic Adenoma of the Larynx: A Case Report

1979 ◽  
Vol 88 (1) ◽  
pp. 112-114 ◽  
Author(s):  
Peter M. Som ◽  
Sidney S. Feuerstein ◽  
Barry D. Nagel ◽  
Lotte Strauss
Keyword(s):  
Salivary Gland ◽  
Pleomorphic Adenoma ◽  
Case Reports ◽  
Minor Salivary Gland ◽  
Salivary Gland Tumors ◽  
Laryngeal Function ◽  
Lateral Pharyngotomy ◽  
Adenoid Cystic ◽  
Minor Salivary Gland Tumors ◽  
Supraglottic Larynx

Minor salivary gland tumors of the larynx are relatively rare. Morphologically the majority of them are adenoid cystic carcinomas and the overwhelming location is the subglottic larynx. Benign pleomorphic adenomas are rare minor salivary gland tumors of the larynx which usually occur at the glottic and subglottic levels. We present only the sixth case of a supraglottic benign pleomorphic adenoma that we could find in the literature. A preoperative laryngogram localized the tumor to the supraglottic larynx and the intact mucosa suggested a specific differential diagnosis. The tumor was removed by a lateral pharyngotomy with retention of good laryngeal function. The relatively asymptomatic presentation of our case is a somewhat unusual finding which, however, was previously noted in similar case reports.

scholarly journals RAMAN SPECTROSCOPY OF HUMAN HEMOGLOBIN FOR DIABETES DETECTION

2014 ◽  
Vol 07 (01) ◽  
pp. 1350051 ◽  
Author(s):  
JUQIANG LIN ◽  
JINYONG LIN ◽  
ZUFANG HUANG ◽  
PENG LU ◽  
JING WANG ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Roc Curve ◽  
Principal Component ◽  
Diagnostic Algorithm ◽  
Noninvasive Detection ◽  
Human Hemoglobin ◽  
Linear Discriminant ◽  
Patients With Diabetes ◽  
The Difference ◽  
Diabetes Monitoring

Glycated hemoglobin (HbA1c) has been increasingly accepted as the gold standard for diabetes monitoring. In this study, Raman spectroscopy was tentatively employed for human hemoglobin (Hb) biochemical analysis aimed at developing a simple blood test for diabetes monitoring. Raman spectroscopy measurements were performed on hemoglobin samples of patients (n = 39) with confirmed diabetes and healthy volunteers (n = 37). The tentative assignments of the measured Raman bands were performed to compare the difference between these two groups. Meanwhile, principal component analysis (PCA) combined with linear discriminant analysis (LDA) were employed to develop effective diagnostic algorithms for classification between normal controls and patients with diabetes. As a result, the spectral features of these two groups demonstrated two distinct clusters with a sensitivity and specificity of 92.3% and 73%, respectively. Then the effectiveness of the diagnostic algorithm based on PCA-LDA technique was confirmed by receiver operating characteristic (ROC) curve. The area under the ROC curve was 0.92, indicating a good diagnostic result. In summary, our preliminary results demonstrate that proposing Raman spectroscopy can provide a significant potential for the noninvasive detection of diabetes.

scholarly journals Raman Spectroscopy Analysis for Optical Diagnosis of Oral Cancer Detection

2019 ◽  
Vol 8 (9) ◽  
pp. 1313 ◽  
Author(s):  
Ming-Jer Jeng ◽  
Mukta Sharma ◽  
Lokesh Sharma ◽  
Ting-Yu Chao ◽  
Shiang-Fu Huang ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Discriminant Analysis ◽  
Oral Cancer ◽  
Cross Validation ◽  
Principal Component ◽  
Beta Carotene ◽  
Invasive Technique ◽  
Protein Amino Acid ◽  
Linear Discriminant ◽  
Model Studies

Raman spectroscopy (RS) is widely used as a non-invasive technique in screening for the diagnosis of oral cancer. The potential of this optical technique for several biomedical applications has been proved. This work studies the efficacy of RS in detecting oral cancer using sub-site-wise differentiation. A total of 80 samples (44 tumor and 36 normal) were cryopreserved from three different sub-sites: The tongue, the buccal mucosa, and the gingiva of the oral mucosa during surgery. Linear discriminant analysis (LDA) and quadratic discriminant analysis (QDA) were used with principal component analysis (PCA) to classify the samples and the classifications were validated by leave-one-out-cross-validation (LOOCV) and k-fold cross-validation methods. The normal and tumor tissues were differentiated under the PCA-LDA model with an accuracy of 81.25% (sensitivity: 77.27%, specificity: 86.11%). The PCA-QDA classifier model differentiated these tissues with an accuracy of 87.5% (sensitivity: 90.90%, specificity: 83.33%). The PCA-QDA classifier model outperformed the PCA-LDA-based classifier. The model studies revealed that protein, amino acid, and beta-carotene variations are the main biomolecular difference markers for detecting oral cancer.

scholarly journals Tenascin and fibronectin in pleomorphic adenoma of the salivary gland

2006 ◽  
Vol 14 (3) ◽  
pp. 198-202 ◽  
Author(s):  
Patrícia Meira Bento ◽  
Roseana de Almeida Freitas ◽  
Leão Pereira Pinto ◽  
Lélia Batista de Souza
Keyword(s):  
Salivary Gland ◽  
Minor Salivary Gland ◽  
Salivary Gland Tumors ◽  
Case Methods ◽  
Myxoid Stroma ◽  
Morphological Findings ◽  
Extracellular Matrix Components ◽  
Minor Salivary Gland Tumors ◽  
Pleomorphic Adenomas ◽  
Different Types

OBJECTIVES: To analyze the expression and distribution pattern of extracellular matrix components in pleomorphic adenomas of the major and minor salivary glands and to compare the morphological findings of these tumors with the immunohistochemical expression, considering the different types of stroma predominating in each case. METHODS AND RESULTS: The expression of tenascin (TN) and fibronectin (FN) was analyzed in 23 cases of pleomorphic adenomas, 11 major and 12 minor salivary gland tumors, by the streptavidin-biotin method using anti-tenascin and anti-fibronectin antibodies. In addition, the immunohistochemical results were correlated with the morphological findings of the lesions. All cases analyzed were immunoreactive for the antibodies used. Fibronectin showed strong labeling in fibrous and chondroid stroma, while labeling was weak in hyaline and myxoid stroma. Tenascin expression was more intense in fibrous and chondroid stroma and moderate in hyaline and myxoid stroma. CONCLUSIONS: No difference in the expression of these proteins was observed between major and minor salivary gland tumors.

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