Serum miR-34a-5p and miR-199a-3p as new biomarkers of neonatal sepsis

Background Neonatal sepsis is a serious condition. Recent clinical studies have indicated that microRNAs (miRNAs) are key players in the pathogenesis of sepsis, which could be used as biomarkers for this condition. Patients and methods A total of 90 neonates with sepsis and 90 healthy neonates were enrolled in this study. qRT-PCR was performed to measure the expression levels of serum miR-34a-5p and miR-199a-3p. Results miR-34a-5p and miR-199a-3p serum levels were significantly reduced in neonates with sepsis compared with those in healthy neonates (P = 0.006 and P = 0.001, respectively). Significant correlations of miR-34a-5p and miR-199a-3p with each of TLC, RDW, RBS, and C-reactive protein (CRP) as well as SNAPII were observed, indicating their associations with the severity of neonatal sepsis. Conclusion miR-34a-5p and miR-199a-3p may be useful as novel biomarkers in neonatal sepsis and may provide a new direction for its treatment.

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C-reactive protein and cytokines in the diagnosis of neonatal sepsis

Medicinski pregled ◽

10.2298/mpns0612545j ◽

2006 ◽

Vol 59 (11-12) ◽

pp. 545-549 ◽

Cited By ~ 2

Author(s):

Borisav Jankovic ◽

Dobrila Veljkovic ◽

Srdjan Pasic ◽

Zorica Rakonjac ◽

Dragana Jevtic ◽

...

Keyword(s):

Acute Phase ◽

Neonatal Sepsis ◽

Current Knowledge ◽

Systemic Infection ◽

Serum Levels ◽

C Reactive Protein ◽

Correct Treatment ◽

Acute Phase Reactants ◽

Reactive Protein ◽

Serum Crp

Introduction. Accurate evaluation and correct treatment of neonates for possible sepsis still represent the most challenging clinical tasks. Early diagnosis of neonatal sepsis is largely based on the measurement of serum concentrations of different mediators of systemic inflammation, as well as, on a group of proteins named acute phase reactants. Among acute phase reactants, C-reactive protein (CRP) has been the most extensively used and investigated so far. Synthesis and biological role of CRP. This article reviews current knowledge on the synthesis, structure and biologic roles of CRP. Also, we present our original results in regard to the kinetics of serum CRP concentration during the first 24 hours of systemic infection, as well as different patterns of CRP dynamics associated with the initial choice of antibiotics, complications and the final outcome of systemic infection. Interleukins and procalcitonin in diagnosis of sepsis. Because CRP is specific, but somewhat late marker of neonatal sepsis, possible diagnostic use of other indicators of inflammation, i.e. interleukins 6 and 8, and procalcitonin during neonatal sepsis is also considered. The theoretical advantage of these early indicators is discussed in comparative analysis of the time of their activation after initial infectious stimuli. Conclusion. In conclusion, we point to the diagnostic accuracy of serial measurements of serum CRP levels. As an alternative, simultaneous measurement of CRP and serum levels using a faster marker, such as procalcitonin, is recommended.

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Serum Interleukin-33 as a Biomarker in Predicting Neonatal Sepsis in Premature Infants

Combinatorial Chemistry & High Throughput Screening ◽

10.2174/1386207321666180911090656 ◽

2018 ◽

Vol 21 (7) ◽

pp. 510-515 ◽

Cited By ~ 3

Author(s):

Halit Halil ◽

Cuneyt Tayman ◽

Mehmet Buyuktiryaki ◽

Nilufer Okur ◽

Ufuk Cakır ◽

...

Keyword(s):

Interleukin 6 ◽

Neonatal Sepsis ◽

Serum Levels ◽

Control Group ◽

Rapid Progression ◽

Study Group ◽

C Reactive Protein ◽

Mortality And Morbidity ◽

Interleukin 33 ◽

Reactive Protein

Background: Neonatal sepsis is considered as the most frequent cause of death in newborns. Early diagnosis is important to reduce mortality and morbidity. The rapid progression of the disease requires proper use of biomarkers specific for prompt diagnosis and intervention. Objective: We aimed to evaluate the benefit of interleukin-33 serum levels in the diagnosis and treatment of neonatal sepsis. Method: We included 51 infants with neonatal sepsis as the main study group and 50 neonates without sepsis as the control group. Serum levels of interleukin-6, interleukin-33 and C-reactive protein were measured on the 1st, 3rd and 7th days of sepsis in the study group and on the 3rd postpartum day in the control group, respectively. Results: Serum levels of interleukin-6, interleukin-33 and C-reactive protein were significantly higher in the first day of sepsis. Serum levels of interleukin-6, interleukin-33 and C-reactive protein decreased significantly on the 3rd and the 7th days of antibiotic treatment. We found a significant relationship between interleukin-33 and C-reactive protein and between interleukin-6 and C-reactive protein on the first day of sepsis. Conclusion: Serum interleukin-33 level is up-regulated in neonatal sepsis, which might be used as a novel diagnostic marker and also a useful tool to predict prognosis in early neonatal sepsis.

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855 Both ramipril and telmisartan reduce serum levels of high-sensitivity C-reactive protein without affecting low-density lipoprotein cholesterol oxidation in patients with type II diabetes mellitus

European Heart Journal ◽

10.1016/s0195-668x(03)94193-5 ◽

2003 ◽

Vol 24 (5) ◽

pp. 145 ◽

Cited By ~ 1

Author(s):

S KOULOURIS

Keyword(s):

Type Ii Diabetes ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

High Sensitivity ◽

Serum Levels ◽

Type Ii Diabetes Mellitus ◽

Cholesterol Oxidation ◽

C Reactive Protein ◽

Low Density Lipoprotein Cholesterol ◽

Reactive Protein

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Diagnosis of neonatal sepsis in low resource settings: C-reactive protein or procalcitonin?

Journal of Pediatric Biochemistry ◽

10.1055/s-0036-1586432 ◽

2016 ◽

Vol 03 (02) ◽

pp. 079-083

Author(s):

Lawrence Mbuagbaw ◽

Francisca Monebenimp ◽

Bolaji Obadeyi ◽

Grace Bissohong ◽

Marie-Thérèse Obama ◽

...

Keyword(s):

Neonatal Sepsis ◽

C Reactive Protein ◽

Low Resource Settings ◽

Low Resource ◽

Reactive Protein

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Brain-derived neurotrophic factor is associated with coronary macrophage infiltrates in patients with acute coronary syndrome

European Heart Journal ◽

10.1093/ehjci/ehaa946.1300 ◽

2020 ◽

Vol 41 (Supplement_2) ◽

Author(s):

R.A Montone ◽

M Camilli ◽

M Russo ◽

M Del Buono ◽

F Gurguglione ◽

...

Keyword(s):

Acute Coronary Syndrome ◽

Neurotrophic Factor ◽

Serum Levels ◽

Brain Derived Neurotrophic Factor ◽

C Reactive Protein ◽

St Segment Elevation ◽

Protein Levels ◽

Reactive Protein ◽

Coronary Syndrome ◽

St Segment

Abstract Background Brain-derived neurotrophic factor (BDNF) is a neurotrophine that plays a key role in the regulation of both central and peripheral nervous system. Moreover, BDNF is secreted in multiple tissues and exerts systemic, autocrine, and paracrine effects in the cardiovascular system. Of importance, BDNF expression was enhanced in macrophages and smooth muscle cells in atherosclerotic coronary arteries and may be involved in thrombus formation. Thus, BDNF has been suggested as an important link between inflammation and thrombosis, potentially involved in the pathogenesis of acute coronary syndrome (ACS). Purpose In our study we aimed at assessing serum levels of BDNF in patients with ACS, evaluating differences according to clinical presentation [ST-segment elevation myocardial infarction (STEMI) vs. Non-ST-segment elevation ACS (NSTE-ACS)]. Moreover, we assessed the presence of optical coherence (OCT)-defined macrophage infiltrates (MØI) in the culprit vessel of ACS patients and evaluated their relationship with BDNF levels. Methods ACS patients were prospectively selected. Blood samples were collected at admission and serum levels of BDNF were subsequently assessed. Presence of OCT-defined MØI along the culprit vessel was assessed. Results 166 ACS patients were enrolled [mean age 65.3±11.9 years, 125 (75.3%) male, 109 STEMI, 57 NSTE-ACS]. Serum levels of BDNF were higher among STEMI patients compared with NSTE-ACS [median (IQR) 2.48 pg/mL (1.54–3.34) vs. 2.12 pg/mL (1.34–2.47), p=0.007], while C-reactive protein levels did not differ between the two groups. OCT assessment was performed in 53 patients and MØI were detected in 27 patients. Of importance, patients with MØI in the culprit vessel had higher levels of BDNF compared with patients without MØI [median (IQR) 2.23 pg/mL (1.38–2.53) vs. 1.41 pg/mL (0.93–2.07), p=0.023], while C-reactive protein levels did not differ between the two groups. Of note, at multivariate regression analysis BDNF levels were independent predictor of MØI [OR: 2.20; 95% CI (1.02–4.74), p=0.043]. Conclusions Serum levels of BDNF may reliable identify the presence of local macrophage inflammatory infiltrates in patients with ACS. Moreover, BDNF levels are higher in patients with STEMI compared with NSTE-ACS. Taken together, these data suggest that BDNF may represent an interesting link between local inflammatory activation and enhanced thrombosis in ACS. BDNF serum levels Funding Acknowledgement Type of funding source: None

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Serum C-reactive protein and WBC count in conservatively and operatively managed bacterial spondylodiscitis

Journal of Orthopaedic Surgery ◽

10.1177/2309499020968296 ◽

2020 ◽

Vol 29 (1) ◽

pp. 230949902096829

Author(s):

Christina van Gerven ◽

Kevin Eid ◽

Tobias Krüger ◽

Michael Fell ◽

Daniel Kendoff ◽

...

Keyword(s):

Antibiotic Treatment ◽

Serum Levels ◽

Blood Chemistry ◽

Nonoperative Treatment ◽

C Reactive Protein ◽

Disc Disease ◽

Reactive Protein ◽

Postoperative Serum ◽

Wbc Count ◽

And Control

Purpose: C-reactive protein (CRP) and white blood cell (WBC) count are routine blood chemistry parameters in monitoring infection. Little is known about the natural history of their serum levels in conservative and operative spondylodiscitis treatment. Methods: Pre- and postoperative serum levels of CRP and WBC count in 145 patients with spondylodiscitis were retrospectively assessed. One hundred and four patients were treated by debridement, spondylodesis, and an antibiotic regime, 41 only with a brace and antibiotics. The results of the surgical group were compared to 156 patients fused for degenerative disc disease (DDD). Results: Surgery had a significant effect on peak postoperative CRP levels. In surgically managed patients, CRP peaked at 2–3 days after surgery (spondylodiscitis: pre-OP: 90 mg/dl vs. post-OP days 2–3: 146 mg/dl; DDD: 9 mg/dl vs. 141 mg/dl; p < 0.001), followed by a sharp decline. Although values were higher for spondylodiscitis patients, dynamics of CRP values were similar in both groups. Nonoperative treatment showed a slower decline. Surgically managed spondylodiscitis showed a higher success rate in identifying bacteria. Specific antibiotic treatment led to a more predictable decline of CRP values. WBC did not show an interpretable profile. Conclusion: CRP is a predictable serum parameter in patients with spondylodiscitis. WBC count is unspecific. Initial CRP increase after surgery is of little value in monitoring infection. A preoperative CRP value, and control once during the first 3 days after surgery is sufficient. Closer monitoring should then be continued. Should a decline not be observed, therapy needs to be scrutinized, antibiotic treatment reassessed, and concomitant infection contemplated.

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