scholarly journals Unveiling biogeographical patterns of the ichthyofauna in the Tuichi basin, a biodiversity hotspot in the Bolivian Amazon, using environmental DNA

PLoS ONE ◽  
2022 ◽  
Vol 17 (1) ◽  
pp. e0262357
Author(s):  
Cédric Mariac ◽  
Fabrice Duponchelle ◽  
Guido Miranda ◽  
Camila Ramallo ◽  
Robert Wallace ◽  
...  

To date, more than 2400 valid fish species have been recorded in the Amazon basin. However, some regions remain poorly documented. This is the case in the Beni basin and in particular in one of its main sub-basins, the Tuichi, an Andean foothills rivers flowing through the Madidi National Park in the Bolivian Amazonia. The knowledge of its ichthyological diversity is, however, essential for the management and protection of aquatic ecosystems, which are threatened by the development of infrastructures (dams, factories and cities), mining and deforestation. Environmental DNA (eDNA) has been relatively little used so far in the Amazon basin. We sampled eDNA from water in 34 sites in lakes and rivers in the Beni basin including 22 sites in the Tuichi sub-basin, during the dry season. To assess the biogeographical patterns of the amazonian ichthyofauna, we implemented a metabarcoding approach using two pairs of specific primers designed and developed in our laboratory to amplify two partially overlapping CO1 fragments, one of 185bp and another of 285bp. We detected 252 fish taxa (207 at species level) among which 57 are newly identified for the Beni watershed. Species compositions are significantly different between lakes and rivers but also between rivers according to their hydrographic rank and altitude. Furthermore, the diversity patterns are related to the different hydro-ecoregions through which the Tuichi flows. The eDNA approach makes it possible to identify and complete the inventory of the ichthyofauna in this still poorly documented Amazon basin. However, taxonomic identification remains constrained by the lack of reference barcodes in public databases and does not allow the assignment of all OTUs. Our results can be taken into account in conservation and management strategies and could serve as a baseline for future studies, including on other Andean tributaries.

2017 ◽  
Vol 74 (12) ◽  
pp. 2030-2034 ◽  
Author(s):  
Stephen L. Klobucar ◽  
Torrey W. Rodgers ◽  
Phaedra Budy

Environmental DNA (eDNA) sampling has proven to be a valuable tool for detecting species in aquatic ecosystems. Within this rapidly evolving field, a promising application is the ability to obtain quantitative estimates of relative species abundance based on eDNA concentration rather than traditionally labor-intensive methods. We investigated the relationship between eDNA concentration and Arctic char (Salvelinus alpinus) abundance in five well-studied natural lakes; additionally, we examined the effects of different temporal (e.g., season) and spatial (e.g., depth) scales on eDNA concentration. Concentrations of eDNA were linearly correlated with char population estimates ([Formula: see text] = 0.78) and exponentially correlated with char densities ([Formula: see text] = 0.96 by area; 0.82 by volume). Across lakes, eDNA concentrations were greater and more homogeneous in the water column during mixis; however, when stratified, eDNA concentrations were greater in the hypolimnion. Overall, our findings demonstrate that eDNA techniques can produce effective estimates of relative fish abundance in natural lakes. These findings can guide future studies to improve and expand eDNA methods while informing research and management using rapid and minimally invasive sampling.


1995 ◽  
Vol 2 (3) ◽  
pp. 244 ◽  
Author(s):  
G. R. Friend ◽  
M. R. Williams

A three-year study (1989?92) of the responses of invertebrates to fire was carried out in mallee-heath shrublands in the Stirling Range National Park, Western Australia. Abundances were measured at the Order level for major groups, and at the morphospecies level for Coleoptera. Changes in floristics and vegetation structure were monitored over the same period. At the Order level, variation in abundances was attributable more to locality, seasonal and year-to-year effects than to fire. Responses of beetles at the morphospecies level, however, reflected changes due to fire as well those attributable to season and year. Coleoptera and Diptera were most abundant 40 years after fire, Hemiptera and Orthoptera peaked in earlier seral stages, while Hymenoptera and Araneae showed relatively few fire-related trends. Coleoptera and Diptera best reflected changes due to season, year and fire, and together with some Araneae such as mygalomorph spiders, would most likely be suitable groups to consider in future studies. Multivariate analyses indicated that classification to morphospecies level is essential to elucidate changes due to fire. These analyses also indicated that changes in invertebrate abundance and composition did not accord with changes in floristics or vegetation structure. Each set of data therefore represents different facets of change over time, including those due to fire. Comparing our data with contemporaneous information collected in upland areas of the Park indicates that there is a clear dichotomy in the fire sensitivity of species inhabiting the wet gullies and thickets of the mountains and those occupying the seasonally dry lowland mallee-heaths. Fire management strategies need to take account of this dichotomy by protecting relictual fire sensitive species and habitats.


2021 ◽  
Vol 13 (11) ◽  
pp. 6478
Author(s):  
Amemarlita Matos ◽  
Laura Barraza ◽  
Isabel Ruiz-Mallén

This study is based on ethnographic research that analyzes how traditional knowledge and local beliefs on biodiversity conservation relates to the local ability to adapt and be resilient to climatic changes in two communities around Gorongosa National Park, Mozambique: Nhanfisse in the buffer zone and Muanandimae in the core area. A total of 78 semi-structured interviews with heads of households were conducted. We found that both communities carried out practices and held beliefs associated with conservation, such as protecting trees and animal species considered sacred or perceived as beneficial for human life in terms of water provision and agricultural production. In addition to traditional ceremonies that respond to extreme climatic events such as drought and flood, other adaptation strategies used by the communities include moving to neighboring areas in search of better living conditions and using forest products in times of scarcity. We discuss that the management of the park should be agreed on, in a shared way, between local communities and conservation agents to ensure that these areas continue to perform the ecological, subsistence, and spiritual functions required. Our research results contribute to a better understanding of local adaptation dynamics towards extreme climatic events and improvement of management strategies.


Insects ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 266
Author(s):  
Enrique Baquero ◽  
Rafael Jordana ◽  
Vicente M. Ortuño

Megalothorax minimus (Neelidae) and Sphaeridia pumilis (Sminthurididae) had already been identified in surface sampling from Sierra de Guadarrama. In Europe, Sminthurinus gisini (Katiannidae) seems to be associated with environments at specific altitudes, and has little representation in this sampling. Pygmarrhopalites custodum Baquero and Jordana sp. nov. (Arrhopalitidae) coexists with two previously identified surface occurring species of the same family (P. elegans and Arrhopalites caecus). However, P. custodum is more abundant, indicating that it occupies an ecological niche tending to troglophile in the mesovoid shallow substratum (MSS). Moreover, it is also more abundant in the MSS of higher altitude corresponding to the bioclimatic zones cryo-oro-Mediterranean and oro-Mediterranean supra forest. Allacma cryptica Baquero and Jordana sp. nov. (Sminthuridae), is another species that had not been previously detected on the surface in the study area. A. cryptica is an addition to a genus which has eight described species. Gisinurus malatestai (Sminthuridae) appears well represented in the MSS, being a species present very occasionally in the Mediterranean area. Two species of the genera Sminthurides (Sminthurididae) and Fasciosminthurus (Bourletiellidae) have been found, but they could not have been identified to the species level. Finally, a few specimens of Dicyrtomina minuta (Dicyrtomidae), an abundant species on the surface, have been captured.


2021 ◽  
Vol 7 (1) ◽  
pp. 54
Author(s):  
Eduardo Bagagli ◽  
Daniel Ricardo Matute ◽  
Hans Garcia Garces ◽  
Bernardo Guerra Tenório ◽  
Adalberto Garcia Garces ◽  
...  

Paracoccidioidomycosis is an endemic fungal disease to Latin America caused by at least five species-level genotypes of Paracoccidioides, named P. lutzii, P. brasiliensis (S1a and S1b populations), P. americana, P. restrepiensis, and P. venezuelensis. In this manuscript, we report on Paracoccidioides sp. sampling efforts in armadillos from two different areas in Brazil. We sequenced the genomes of seven Paracoccidioides isolates and used phylogenomics and populations genetics for genotyping. We found that P. brasiliensis and P. lutzii are both present in the Amazon region. Additionally, we identified two Paracoccidioides isolates that seem to be the result of admixture between divergent populations within P. brasiliensis sensu stricto. Both of these isolates were recovered from armadillos in a P. lutzii endemic area in Midwestern Brazil. Additionally, two isolates from human patients also show evidence of resulting from admixture. Our results suggest that the populations of P. brasiliensis sensu stricto exchange genes in nature. More generally, they suggest that population structure and admixture within species is an important source of variation for pathogenic fungi.


2017 ◽  
Vol 44 (1) ◽  
pp. 28 ◽  
Author(s):  
N. T. Maruping-Mzileni ◽  
P. J. Funston ◽  
S. M. Ferreira

Aims Indicators of pending state-shifts carry value for policy makers. Predator–prey relations reflect key ecological processes that shape ecosystems. Variance in predator–prey relations may serve as a key indicator of future state-shifts. Methods Lion (Panthera leo) diet in the Kruger National Park was evaluated as such an indicator. Over the three-decade time span reviewed, variance in diet in relation to rainfall, prey abundance, management strategies and disease emergence were reviewed. Key results Rainfall patterns, both seasonal and cyclical, were identified as key drivers of predator–prey selection. However, the intensity of management in the form of artificial waterpoints overrode and confounded natural process. The results suggest that savanna systems are stable and punctuated by climatic events in the form of extreme above-average rainfall that temporarily destabilises the system. However, droughts are a cyclical part of the savanna system. Conclusion Lion prey selection did fluctuate with changing environmental conditions. Abrupt state shifts did occur; however, the ecosystem returned to a stable state. Implications State shifts in ecosystems pose key challenges to conservation managers. State shifts appear to be primarily associated with management interventions and environmental factors.


Oryx ◽  
2017 ◽  
Vol 53 (4) ◽  
pp. 727-731 ◽  
Author(s):  
Malcolm S. Ramsay ◽  
Andriamahery Razafindrakoto ◽  
Shawn M. Lehman

AbstractAlthough roads are often assumed to be barriers to the dispersal of arboreal species, there has been little empirical testing of this assumption. If arboreal animals are unable to cross roads, population subdivision may occur, or resources may become inaccessible. We tested the hypothesis that Route Nationale 4 (RN4), a paved highway, was a barrier to movement and dispersal of the Endangered golden-brown mouse lemur Microcebus ravelobensis in Ankarafantsika National Park, in north-west Madagascar. During June–August 2015 we conducted a capture–mark–recapture study at three sites: two adjacent to RN4 and one within intact forest without a potential barrier. During 2,294 trap nights we captured 120 golden-brown mouse lemurs 1,032 times. In roadside habitats we captured significantly more males than females, whereas the opposite was the case in interior forest habitat. We detected eighteen crossings of highway transects by nine individuals; however, all potential dispersal events involved males. In roadside habitat, movement was significantly inhibited in both males and females. We present some of the first data on the effects of roads on movement patterns in arboreal Malagasy mammals, showing species- and sex-biased effects of roads as dispersal barriers. Our findings indicate that roads may not be complete barriers to dispersal in lemurs. We recommend that conservation managers and scientists examine explicitly the effects of roads and natural arboreal bridges in Madagascar in future studies.


2020 ◽  
Vol 8 (2) ◽  
pp. 336
Author(s):  
Maria Feninsia Asni Gabur ◽  
Made Sukana

Padar Island as one of the main tourist attraction is part of Komodo National Park in Labuan Bajo. Its tourism potential become more in demand compared to Komodo and Rinca Island. The practice of tourism in this Komodo National Park area does not readily claim the management of tourism especially. The focuses of the Office Komodo National Park are conservation and preservation.  This study uses primary and secondary data sources with qualitative and quantitative data types. Methods of collecting data through observation, interviews, and documents with the determination of informants using purposive procedure techniques, and use data analysis qualitatively.  The result of this study answer three main problems raised, namely the first on the tourism profile of Padar Island relate to the basis of attraction, accessibility, amenities and ancillary. The second is relate to tourism management including demand and supply, the influence of tourism on natural conditions, forms of interaction of tourist with local communities and benefits gained by the community with tourism activities on Padar Island and the third is relate to resource management strategies on Padar Island by parties Komodo National Park.   Keywords: Profile, Tourism, Management, Strategy, Resource


2019 ◽  
Vol 8 (2) ◽  
pp. 102-110
Author(s):  
Yulia Santi ◽  
Sutrisno Anggoro ◽  
Suryanti Suryanti

ABSTRAKPerikanan  tangkap  di  kawasan  Taman  Nasional  Karimunjawa  dikelola  oleh  lebih  dari  satu  instansipengelola.  Setiap  instansi  diduga  mempunyai  peran  masing-  masing  sesuai  tupoksinya.  Saat  ini  belum diketahui secara nyata instansi apa saja yang mengelola beserta perannya. Tujuan penelitian ini adalah untuk mengidentifikasi instansi pengelola yang berperan dalam pengelolaan perikanan tangkap di kawasan TNKJ, persepsi,  aspirasi  dan  partisipasi  nelayan  terhadap  pengelolaan  perikanan  tangkap  serta  bagaimana  strategi pengelola  dalam  pengawasan  perikanan  tangkap.  Metode  penelitian  ini  adalah  studi  kasus  dengan  analisis deskriptif, dimana pengumpulan data melalui wawancara dan studi pustaka. Penentuan responden menggunakan metode purposive sampling, responden terdiri dari nelayan dan instansi pengelola. Hasil penelitian menunjukan bahwa  instansi yang  mengelola  perikanan tangkap adalah Unit  Pelaksana  Teknis   Pelabuhan Perikanan Pantai Karimunjawa, Balai Taman Nasional Karimunjawa, Dinas Perikanan, Polisi Air   dan Tentara Nasional Indonesia Angkatan Laut dan Satuan Kerja Pengawasan Sumberdaya Kelautan dan   Perikanan. Pembagian peran masing- masing instansi pengelola sudah sesuai dengan bidang masing-masing  serta tidak terjadi tumpang tindih kewenangan. Sinkronisasi dan koordinasi sudah dilakukan, hal ini terbukti   dengan  adanya  Nota  Kesepakatan Bersama. Sebanyak 80% nelayan setuju dengan peraturan yang diterapkan. Partisipasi nelayan terhadap sosialisasi dan pelatihan rendah yaitu 35% dan 19%. Strategi pengelola dalam pengawasan perikanan tangkap adalah dengan membentuk dan memberdayakan Pengawas   Perikanan  dan  Kelompok  Masyarakat  Pengawas  secara  sinergi. Strategi untuk  meningkatkan partisipasi  nelayan  dalam  sosialisasi  dan  pelatihan  adalah  dengan  memberikan penyadaran  motivasi dan  apresiasi berupa penghargaan dan pendampingan. ABSTRACTCapture   fisheries   in Karimunjawa National   Park   are   managed by   more   than one   management institutions.  Each  institution  is  assumed  to  have  their  respective  roles  according  to  their  basic  tasks  andfunctions. It is not yet known exactly what institutions are managing along with their roles. The purposes of  this study  were to know what institutions  were involved  in the management of capture fisheries in TNKJ  areas, perceptions, aspirations and participation of fishermen on the management of capture fisheries and  how the management strategy in the supervision of capture fisheries. This research method was case study with descriptive analysis, where data collected by interview and literature study. Determination of  respondents used purposive sampling method, respondents consist of fishermen and manager institution. The results showed that the institutions that managed capture fisheries were Technical Implementation Unit  Karimunjawa Fishery Port, Karimunjawa National Park Office, Fisheries Official, Water Police and Indonesian National Army – Navy and Work Unit of Supervision of Marine and Fisheries Resources. The  division of roles of each institution agency was in accordance with their respective fields and there was no  overlapping authority. Synchronization and coordination had been done, it was proved by the Memorandum of Understanding (MoU). As many as 80% of fishermen agree with the regulations applied. The  participations of fishermen on socialization and training were low at 35% and 19%. The management strategies in the supervision of capture fisheries were by establishing and empowering Fisheries Supervisor  and a group of supervisor community (PokMasWas). The strategies to increase the participation of fishermen in socialization and training were by giving awareness of motivation and appreciation in the form  of recognition and assistance.


2021 ◽  
Vol 4 ◽  
Author(s):  
O. Nurul Fizatul Nabilah ◽  
A. R. Ramizah ◽  
A. B. Adibah ◽  
S. Syazwan ◽  
A.G. Intan Faraha ◽  
...  

Peacock bass or the cichlids are known locally as top predator fishes which are invasive in Malaysia freshwater system. Detection probabilities for these fishes are typically low, especially using conventional capture-survey method due to the fish’s behaviour of hiding beneath the water’s surface. Hence, the environmental DNA (eDNA) monitoring is a relatively new approach that can be used to assess the distribution of these invasive fishes. Here, we report the strategy to develop small fragment (280- 400 bp) specific-specific primers for three selected invasive Cichla species namely, C. ocellaris, C. monoculus, and C. kelberi based on mitochondrial DNA (mtDNA) sequences. Current research showed that the developed species-specific primers from cytochrome oxidase I (COI) gene has high resolution at species level. Species-specific amplification tests also proved the specificity of the developed primers, securing the high- level species identification potential which may help in controlling the spread of alien invasive fish species.


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