Evaluation of the discriminatory power of spoligotyping and 19-locus mycobacterial interspersed repetitive unit-variable number of tandem repeat analysis (MIRU-VNTR) of Mycobacterium bovis strains isolated from cattle in Algeria

Bovine tuberculosis (bTB) caused by Mycobacterium (M.) bovis and M. caprae is a transmissible disease of livestock, notifiable to the World Organization for Animal Health (OIE). BTB particularly affects cattle and small ruminants and can be transmitted to humans thereby posing a significant threat to veterinary and public health worldwide. M. bovis is the principal cause of bTB in Algeria. In order to better understand the route of spreading and elaborate an eradication program, isolation and characterization of mycobacteria from Algerian cattle was performed. Sixty strains belonging to the M. tuberculosis complex were analyzed by spoligotyping, thereof 42 by 19-locus-MIRU-VNTR-typing. Spoligotyping revealed 16 distinguishable patterns (Hunter-Gaston discriminatory index [HGDI] of 0.8294), with types SB0120 (n = 20) and SB0121 (n = 13) being the most frequent patterns, representing 55% of the strains. Analyses based on 19-locus-MIRU-VNTR yielded 32 different profiles, five clusters and one orphan pattern, showing higher discriminatory power (HGDI = 0.9779) than spoligotyping. Seven VNTR-loci [VNTR 577 (alias ETR C), 2163b (QU11b), 2165 (ETR A), 2461 (ETR B), 3007 (MIRU 27), 2163a (QUB11a) and 3232 (QUB 3232)] were the most discriminative loci (HGDI ˃ 0.50). In conclusion, 19-locus-MIRU-VNTR yielded more information than spoligotyping concerning molecular differentiation of strains and better supports the elucidation of transmission routes of M. bovis between Algerian cattle herds.

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Characterization of Genetic Diversity of Bacillus anthracis in France by Using High-Resolution Melting Assays and Multilocus Variable-Number Tandem-Repeat Analysis

Journal of Clinical Microbiology ◽

10.1128/jcm.05439-11 ◽

2011 ◽

Vol 49 (12) ◽

pp. 4286-4292 ◽

Cited By ~ 17

Author(s):

S. Derzelle ◽

S. Laroche ◽

P. Le Fleche ◽

Y. Hauck ◽

S. Thierry ◽

...

Keyword(s):

Genetic Diversity ◽

High Resolution ◽

Bacillus Anthracis ◽

Tandem Repeat ◽

High Resolution Melting ◽

Variable Number Tandem Repeat ◽

Variable Number ◽

Repeat Analysis

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Multiple-locus variable-number tandem repeat analysis and clinical characterization of Leptospira interrogans canine isolates

Journal of Medical Microbiology ◽

10.1099/jmm.0.000027 ◽

2015 ◽

Vol 64 (3) ◽

pp. 288-294 ◽

Cited By ~ 8

Author(s):

Nobuo Koizumi ◽

Maki Mizutani Muto ◽

Hidemasa Izumiya ◽

Motoi Suzuki ◽

Makoto Ohnishi

Keyword(s):

Tandem Repeat ◽

Variable Number Tandem Repeat ◽

Variable Number ◽

Leptospira Interrogans ◽

Multiple Locus ◽

Repeat Analysis

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Isolation and characterization of dermatophytes from human and small ruminants at Nangarhar and Kunar provinces of Afghanistan

International Journal of Farm Sciences ◽

10.5958/2250-0499.2020.00003.8 ◽

2020 ◽

Vol 10 (1) ◽

pp. 13

Author(s):

Hikmatullah Langar ◽

Mohammad Nazar Fiasal ◽

M Nasim Sohail ◽

D Rathnamma ◽

R Sharada

Keyword(s):

Small Ruminants ◽

Isolation And Characterization

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Characterization of MRSA isolates from Kazakhstan using a multiple locus variable-number tandem repeat analysis

Journal of Microbiology Immunology and Infection ◽

10.1016/j.jmii.2015.02.385 ◽

2015 ◽

Vol 48 (2) ◽

pp. S109

Author(s):

D.B. Babenko ◽

A.V. Lavrinenko ◽

I.S. Azizov

Keyword(s):

Tandem Repeat ◽

Variable Number Tandem Repeat ◽

Variable Number ◽

Multiple Locus ◽

Repeat Analysis

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Characterization of Dutch Staphylococcus aureus from bovine mastitis using a Multiple Locus Variable Number Tandem Repeat Analysis

Veterinary Microbiology ◽

10.1016/j.vetmic.2008.10.034 ◽

2009 ◽

Vol 136 (3-4) ◽

pp. 277-284 ◽

Cited By ~ 36

Author(s):

Risma Ikawaty ◽

E.C. Brouwer ◽

M.D. Jansen ◽

E. van Duijkeren ◽

D. Mevius ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Tandem Repeat ◽

Variable Number Tandem Repeat ◽

Bovine Mastitis ◽

Variable Number ◽

Multiple Locus ◽

Repeat Analysis

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Molecular and serological characterization of pathogenic Leptospira spp. isolated from symptomatic dogs in a highly endemic area, Brazil

BMC Veterinary Research ◽

10.1186/s12917-021-02930-w ◽

2021 ◽

Vol 17 (1) ◽

Author(s):

Cassia Moreira Santos ◽

Gabrielle Cristini Del Rigo Santos Dias ◽

Alexya Victória Pinheiro Saldanha ◽

Stephanie Bergmann Esteves ◽

Adriana Cortez ◽

...

Keyword(s):

Endemic Area ◽

Animal Health ◽

Acute Infection ◽

Serological Tests ◽

Pathogenic Leptospira ◽

Isolation And Characterization ◽

Serological Characterization ◽

Diagnostic Approaches ◽

Veterinary Hospital

Abstract Background Leptospirosis is an endemic zoonosis in Brazil, with a great impact on human and animal health. Although dogs are frequently infected by pathogenic Leptospira, the current epidemiological understanding of canine leptospirosis is mainly based on serological tests that predict the infecting serogroup/serovar. Thus, the present study aimed at identifying the causative agent for severe cases of canine leptospirosis in a highly endemic area through the isolation and characterization of the isolated strains. Results Urine, serum and blood samples were collected from 31 dogs with suspected acute leptospirosis treated at the Veterinary Hospital Service of Santo Amaro University between 2018 and 2019. Acute infection was confirmed in 17 dogs (54.8%) by the associated use of Polymerase Chain Reaction (PCR), Microscopic Agglutination (MAT) and bacteriological culture. Eleven dogs (35.5%) had titers ≥800, with the most frequent serogroups being Autumnalis and Icterohaemorrhagiae (n = 4 each) and Canicola (n = 2). Leptospires were recovered from four dogs, and Multilocus Sequence Analysis (MLSA) revealed infection caused by L. interrogans, which were further characterized as serogroups Canicola (n = 1) and Icterohaemorrhagiae (n = 3). Conclusion The identity of the isolates and serological pattern of MAT suggest that dogs are highly exposed to the serogroup Icterohaemorrhagiae and Canicola, also indicating possible circulation of serogroups not yet isolated in Brazil, notably serogroup Autumnalis. Our findings also reinforce the usefulness of using multiple diagnostic approaches to confirm acute canine leptospirosis.

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Molecular and Serological Characterization of Pathogenic Leptospira Spp. Isolated From Symptomatic Dogs in a Highly Endemic Area, Brazil

10.21203/rs.3.rs-230543/v1 ◽

2021 ◽

Author(s):

Cassia Moreira Santos ◽

Gabrielle Cristini Del Rigo Santos Dias ◽

Alexya Victória Pinheiro Saldanha ◽

Stephanie Bergmann Esteves ◽

Adriana Cortez ◽

...

Keyword(s):

Endemic Area ◽

Animal Health ◽

Acute Infection ◽

Serological Tests ◽

Pathogenic Leptospira ◽

Effective Prevention ◽

Isolation And Characterization ◽

Serological Characterization ◽

Veterinary Hospital

Abstract Background: Leptospirosis is an endemic zoonosis in Brazil, with great impact in human and animal health. Although dogs are frequently infected by pathogenic Leptospira, the current epidemiological understanding of canine leptospirosis is mainly based on serological tests that predict the infecting serogroup/serovar. Thus, the present study aimed at identifying the causative agent for severe cases of canine leptospirosis in a highly endemic area through the isolation and characterization of the isolated strains. Results: Urine, serum and blood samples were collected from 31 dogs with suspected acute leptospirosis treated at the Veterinary Hospital Service of Santo Amaro University between 2018 and 2019. Acute infection was confirmed in 17 dogs (54.8%) by the associated use of Polymerase Chain Reaction (PCR), Microscopic Agglutination (MAT) and bacteriological culture. Eleven dogs (35.5%) had titers ≥800, with the most frequent serogroups being Autumnalis and Icterohaemorrhagiae (n = 4 each) and Canicola (n = 2). Leptospires were recovered from four dogs, and Multilocus Sequence Analysis (MLSA) revealed infection caused by L. interrogans, which were further characterized as serogroups Canicola (n=1) and Icterohaemorrhagiae (n=3). Conclusion: The identification of circulating strains in poverty-stricken locations with humans living in rural-urban interfaces may assist local authorities to implement effective prevention policies. The identity of the isolates and serological pattern of MAT titers found among the suspected dogs suggest that dogs are highly exposed to the serogroup Icterohaemorrhagiae, also indicating possible circulation of serogroups not yet isolated in Brazil, notably serogroup Autumnalis.

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