scholarly journals Quantification of pulsed electric field for the rupture of giant vesicles with various surface charges, cholesterols and osmotic pressures

PLoS ONE ◽  
2022 ◽  
Vol 17 (1) ◽  
pp. e0262555
Author(s):  
Md. Kabir Ahamed ◽  
Marzuk Ahmed ◽  
Mohammad Abu Sayem Karal
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Mole Fraction ◽  
High Frequency ◽  
Pulsed Electric Field ◽  
Surface Charges ◽  
Maximum Probability ◽  
Giant Vesicles ◽  
Biochemical Processes ◽  
Field Dependent

Electropermeabilization is a promising phenomenon that occurs when pulsed electric field with high frequency is applied to cells/vesicles. We quantify the required values of pulsed electric fields for the rupture of cell-sized giant unilamellar vesicles (GUVs) which are prepared under various surface charges, cholesterol contents and osmotic pressures. The probability of rupture and the average time of rupture are evaluated under these conditions. The electric field changes from 500 to 410 Vcm-1 by varying the anionic lipid mole fraction from 0 to 0.60 for getting the maximum probability of rupture (i.e., 1.0). In contrast, the same probability of rupture is obtained for changing the electric field from 410 to 630 Vcm-1 by varying the cholesterol mole fraction in the membranes from 0 to 0.40. These results suggest that the required electric field for the rupture decreases with the increase of surface charge density but increases with the increase of cholesterol. We also quantify the electric field for the rupture of GUVs containing anionic mole fraction of 0.40 under various osmotic pressures. In the absence of osmotic pressure, the electric field for the rupture is obtained 430 Vcm-1, whereas the field is 300 Vcm-1 in the presence of 17 mOsmL-1, indicating the instability of GUVs at higher osmotic pressures. These investigations open an avenue of possibilities for finding the electric field dependent rupture of cell-like vesicles along with the insight of biophysical and biochemical processes.

Electroreflectance and Photoreflectance Studies of Electric Fields in Pt/GaN Schottky Diodes and AlGaN/GaN Heterostructures

2002 ◽  
Vol 743 ◽  
Author(s):  
S. Shokhovets ◽  
R. Goldhahn ◽  
G. Gobsch ◽  
O. Ambacher ◽  
I. P. Smorchkova ◽  
...  
Keyword(s):  
Experimental Data ◽  
Electric Field ◽  
Electric Fields ◽  
Schottky Diodes ◽  
Schottky Contact ◽  
Surface Charges ◽  
Temperature Dependent ◽  
Algan Barrier ◽  
Field Dependent ◽  
Increasing Temperature

ABSTRACTWe have performed electroreflectance and photoreflectance studies of Pt/GaN Schottky diodes with Ga- and N-face polarity as well as AlGaN/GaN based transistor heterostructures. The experimental data were analyzed using electric field-dependent dielectric functions of GaN and AlGaN. Inhomogeneities in the electric fields were taken into account by application of a multi-layer formalism. We observed an increase of the electric field strength underneath the Schottky contact and in the AlGaN barrier with increasing temperature. The results are explained in terms of temperature dependent densities of ionized impurities and surface charges.

scholarly journals Concepts and Capabilities of In-House Built Nanosecond Pulsed Electric Field (nsPEF) Generators for Electroporation: State of Art

2020 ◽  
Vol 10 (12) ◽  
pp. 4244
Author(s):  
Paulius Butkus ◽  
Arūnas Murauskas ◽  
Sonata Tolvaišienė ◽  
Vitalij Novickij
Keyword(s):  
Electric Field ◽  
High Frequency ◽  
Pulsed Electric Field ◽  
Cell Permeabilization ◽  
Pulse Generators ◽  
Nanosecond Pulsed Electric Field ◽  
Forming Characteristics ◽  
State Of Art ◽  
Frequency Pulse

Electroporation is a pulsed electric field triggered phenomenon of cell permeabilization, which is extensively used in biomedical and biotechnological context. There is a growing scientific demand for high-voltage and/or high-frequency pulse generators for electropermeabilization of cells (electroporators). In the scope of this article we have reviewed the basic topologies of nanosecond pulsed electric field (nsPEF) generators for electroporation and the parametric capabilities of various in-house built devices, which were introduced in the last two decades. Classification of more than 60 various nsPEF generators was performed and pulse forming characteristics (pulse shape, voltage, duration and repetition frequency) were listed and compared. Lastly, the trends in the development of the electroporation technology were discussed.

Electric-field-dependent thermal fluctuations of giant vesicles

1993 ◽  
Vol 48 (1) ◽  
pp. 628-631 ◽  
Author(s):  
M. D. Mitov ◽  
P. Méléard ◽  
M. Winterhalter ◽  
M. I. Angelova ◽  
P. Bothorel
Keyword(s):  
Electric Field ◽  
Thermal Fluctuations ◽  
Giant Vesicles ◽  
Field Dependent

Electrohydrodynamic Kelvin-Helmholtz Instability of Two Rotating Dielectric Fluids

1998 ◽  
Vol 53 (1-2) ◽  
pp. 17-26
Author(s):  
Mohamed Fahmy El-Sayed
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Applied Electric Field ◽  
Rotation Frequency ◽  
Short Wave ◽  
Surface Charges ◽  
Rotation Surface ◽  
Dielectric Fluids ◽  
Fluid Velocities ◽  
Conditions Of Stability

Abstract A linear stability analysis of a novel electrohydrodynamic Kelvin-Helmholtz system consisting of the superposition of two uniformly rotating dielectric media is presented. The characteristic equation for such an arrangement is derived, which in turn yields a stability criterion for velocity differences of disturbances at a given rotation frequency. The conditions of stability for long and short wave perturbations are obtained, and their dependence on rotation, surface tension and applied electric field is discussed. Limiting cases for vanishing fluid velocities, rotation frequency, and applied electric field are also discussed. Under suitable limits, results of previous works are recovered. A detailed analysis for tangential and normal applied electric fields, in the presence and absence of surface charges, is carried out.

Pulsed Electric Fields Versus Thermal Treatment: Equivalent Processes To Obtain Equally Acceptable Citrus Juices

2006 ◽  
Vol 69 (8) ◽  
pp. 2016-2018 ◽  
Author(s):  
E. SENTANDREU ◽  
L. CARBONELL ◽  
D. RODRIGO ◽  
J. V. CARBONELL
Keyword(s):  
Thermal Treatment ◽  
Electric Field ◽  
Electric Fields ◽  
Pulsed Electric Fields ◽  
Pectin Methylesterase ◽  
Pulsed Electric Field ◽  
Citrus Juices ◽  
Pulsed Electric Field Treatment ◽  
Electric Field Treatment

Pulsed electric field treatment has been claimed to produce more acceptable chilled citrus juices than those obtained by conventional thermal treatment. The pectin methylesterase activity and the acceptability of nine juices obtained from Clementine mandarins, Valencia oranges, and Ortanique fruits (hybrid of mandarin and orange), untreated, pasteurized (85°C for 10 s), and treated by pulsed electric fields (25 kV/cm for 330 μs), were evaluated. The treatments, selected to reach a similar level of pectin methylesterase inactivation, produced juices that did not differ in acceptability from each other for the three varieties and in all cases were less acceptable than the untreated juice.

scholarly journals Cytoskeletal Disruption after Electroporation and Its Significance to Pulsed Electric Field Therapies

Cancers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1132 ◽  
Author(s):  
Philip M. Graybill ◽  
Rafael V. Davalos
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Irreversible Electroporation ◽  
Pulsed Electric Fields ◽  
Pulsed Electric Field ◽  
Current Understanding ◽  
Vascular Effects ◽  
Cell Substrate ◽  
Membrane Effects ◽  
Cytoskeletal Disruption

Pulsed electric fields (PEFs) have become clinically important through the success of Irreversible Electroporation (IRE), Electrochemotherapy (ECT), and nanosecond PEFs (nsPEFs) for the treatment of tumors. PEFs increase the permeability of cell membranes, a phenomenon known as electroporation. In addition to well-known membrane effects, PEFs can cause profound cytoskeletal disruption. In this review, we summarize the current understanding of cytoskeletal disruption after PEFs. Compiling available studies, we describe PEF-induced cytoskeletal disruption and possible mechanisms of disruption. Additionally, we consider how cytoskeletal alterations contribute to cell–cell and cell–substrate disruption. We conclude with a discussion of cytoskeletal disruption-induced anti-vascular effects of PEFs and consider how a better understanding of cytoskeletal disruption after PEFs may lead to more effective therapies.

Chemical Analysis on the Effect of Pulsed Electric Fields in Pineapple Juices Preservation

2014 ◽  
Vol 554 ◽  
pp. 588-592 ◽  
Author(s):  
Ali Mohammad Dastgheib ◽  
Zolkafle Buntat ◽  
Muhammad Abu Bakar Sidik
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Food Industry ◽  
Pulsed Electric Field ◽  
Liquid Food ◽  
Chemical Parameters ◽  
Natural Properties ◽  
Viable Solution ◽  
Before And After ◽  
Thermal Technique

The application of high voltage electric field for preservation of fruit juices has a promising scope in the food industry. The pulsed electric field (PEF) is an innovative non- thermal technique and free from bio-toxic effects. The technique has a viable solution of the problem yet faced in the food industry to prolong life and preserve and maintain quality with natural properties of the liquid food and beverages. In this study, we have treated the pineapples juice samples by different strengths of pulsed electric field such as 10, 20 and 30kV/cm for 5 minutes in each test. This study used new design of helix treatment chamber with three different lengths of 20, 30and 50cm. In these experiments, all samples were kept in same and normal condition with a temperature around 25-26 andthe humidity was between 55 and 65%. Then the observation based on chemical tests such as pH, conductivity, salinity and total dissolved solids (TDS) was recorded for all samples before and after the test. Based on results obtained by chemical parameters suggest that the injection on pulsed electric field of 30 kV/cm by the 50 cm treatment chamber has the best effect on pineapple juices characteristic as compared to the other value. The result of this experiment is encouraging and supportive of the better way for pasteurization the pineapple juices and increasing longevity of pineapple juices.

Characterization of interactive force acting on colloidal particles near an electrode in presence of a high-frequency (>10 kHz) AC electric field using particle diffusometry

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Kshitiz Gupta ◽  
Dong Hoon Lee ◽  
Steven T. Wereley ◽  
Stuart J. Williams
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Particle Motion ◽  
Electrode Surface ◽  
High Frequency ◽  
Colloidal Particles ◽  
Low Frequency ◽  
Double Layers ◽  
Average Height ◽  
Ac Electric Field

Colloidal particles like polystyrene beads and metallic micro and nanoparticles are known to assemble in crystal-like structures near an electrode surface under both DC and AC electric fields. Various studies have shown that this self-assembly is governed by a balance between an attractive electrohydrodynamic (EHD) force and an induced dipole-dipole repulsion (Trau et al., 1997). The EHD force originates from electrolyte flow caused by interaction between the electric field and the polarized double layers of both the particles and the electrode surface. The particles are found to either aggregate or repel from each other on application of electric field depending on the mobility of the ions in the electrolyte (Woehl et al., 2014). The particle motion in the electrode plane is studied well under various conditions however, not as many references are available in the literature that discuss the effects of the AC electric field on their out-of-plane motion, especially at high frequencies (>10 kHz). Haughey and Earnshaw (1998), and Fagan et al. (2005) have studied the particle motion perpendicular to the electrode plane and their average height from the electrode mostly in presence of DC or low frequency AC (<1 kHz) electric field. However, these studies do not provide enough insight towards the effects of high frequency (>10 kHz) electric field on the particles’ motion perpendicular to the electrode plane.  

Possibility of Microwave-Controlled Surface Modification

1996 ◽  
Vol 430 ◽  
Author(s):  
K. I. Rybakov ◽  
V. E. Semenov
Keyword(s):  
Surface Modification ◽  
Electric Field ◽  
Mass Transport ◽  
Electric Fields ◽  
High Frequency ◽  
Theoretical Study ◽  
Surface Effects ◽  
Moderate Intensity ◽  
Crystalline Solids ◽  
Periodic Profile

AbstractResults of the theoretical study of surface effects in ionic crystalline solids under the action of high-frequency electric fields of moderate intensity are presented. The averaged ponderomotive action of the electric field on the charged vacancies within the crystal causes directional mass transport that leads to development of a surface instability. The analysis shows that the proposed effect can result in the formation of a periodic profile on the surface.

Sign in / Sign up

Export Citation Format

Share Document