Synthetic Genetic Array Analysis in Saccharomyces cerevisiae

2005 ◽  
pp. 171-192 ◽  
Author(s):  
Amy Hin Yan Tong ◽  
Charles Boone
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Synthetic Genetic Array ◽  
Array Analysis

scholarly journals Synthetic Genetic Array Analysis in Saccharomyces cerevisiae Provides Evidence for an Interaction Between RAT8/DBP5 and Genes Encoding P-Body Components

Genetics ◽  
2008 ◽  
Vol 179 (4) ◽  
pp. 1945-1955 ◽  
Author(s):  
John J. Scarcelli ◽  
Susan Viggiano ◽  
Christine A. Hodge ◽  
Catherine V. Heath ◽  
David C. Amberg ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Synthetic Genetic Array ◽  
Array Analysis ◽  
Genes Encoding ◽  
Body Components

Synthetic Genetic Array Analysis

2016 ◽  
Vol 2016 (4) ◽  
pp. pdb.prot088807 ◽  
Author(s):  
Elena Kuzmin ◽  
Michael Costanzo ◽  
Brenda Andrews ◽  
Charles Boone
Keyword(s):  
Synthetic Genetic Array ◽  
Array Analysis

eSGA: E. coli Synthetic Genetic Array analysis

2008 ◽  
Author(s):  
Gareth Butland ◽  
Mohan Babu ◽  
Jack Greenblatt ◽  
Andrew Emili
Keyword(s):  
Synthetic Genetic Array ◽  
Array Analysis ◽  
E Coli

eSGA: E. coli synthetic genetic array analysis

2008 ◽  
Vol 5 (9) ◽  
pp. 789-795 ◽  
Author(s):  
Gareth Butland ◽  
Mohan Babu ◽  
J Javier Díaz-Mejía ◽  
Fedyshyn Bohdana ◽  
Sadhna Phanse ◽  
...  
Keyword(s):  
Synthetic Genetic Array ◽  
Array Analysis ◽  
E Coli

scholarly journals Comprehensive synthetic genetic array analysis of alleles that interact with mutation of the Saccharomyces cerevisiae RecQ helicases Hrq1 and Sgs1

2020 ◽  
Author(s):  
Elsbeth Sanders ◽  
Phoebe A. Nguyen ◽  
Cody M. Rogers ◽  
Matthew L. Bochman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Single Gene ◽  
Colony Growth ◽  
Strand Break ◽  
Telomere Maintenance ◽  
Temperature Sensitive ◽  
Recq Helicase ◽  
Synthetic Genetic Array ◽  
Sensitive Allele ◽  
Dna Maintenance

ABSTRACTMost eukaryotic genomes encode multiple RecQ family helicases, including five such enzymes in humans. For many years, the yeast Saccharomyces cerevisiae was considered unusual in that it only contained a single RecQ helicase, named Sgs1. However, it has recently been discovered that a second RecQ helicase, called Hrq1, resides in yeast. Both Hrq1 and Sgs1 are involved in genome integrity, functioning in processes such as DNA inter-strand crosslink repair, double-strand break repair, and telomere maintenance. However, it is unknown if these enzymes interact at a genetic, physical, or functional level as demonstrated for their human homologs. Thus, we performed synthetic genetic array (SGA) analyses of hrq1Δ and sgs1Δ mutants. As inactive alleles of helicases can demonstrate dominant phenotypes, we also performed SGA analyses on the hrq1-K318A and sgs1-K706A ATPase/helicase-null mutants, as well as all combinations of deletion and inactive double mutants. We crossed these eight query strains (hrq1Δ, sgs1Δ, hrq1-K318A, sgs1-K706A, hrq1Δ sgs1Δ, hrq1Δ sgs1-K706A, hrq1-K318A sgs1Δ, and hrq1-K318A sgsl-K706A) to the S. cerevisiae single gene deletion and temperature-sensitive allele collections to generate double and triple mutants and scored them for synthetic positive and negative genetic effects based on colony growth. These screens identified hundreds of synthetic interactions, supporting the known roles of Hrq1 and Sgs1 in DNA repair, as well as suggesting novel connections to rRNA processing, mitochondrial DNA maintenance, transcription, and lagging strand synthesis during DNA replication.

Synthetic Genetic Array Analysis for Global Mapping of Genetic Networks in Yeast

2014 ◽  
pp. 143-168 ◽  
Author(s):  
Elena Kuzmin ◽  
Sara Sharifpoor ◽  
Anastasia Baryshnikova ◽  
Michael Costanzo ◽  
Chad L. Myers ◽  
...  
Keyword(s):  
Genetic Networks ◽  
Synthetic Genetic Array ◽  
Array Analysis ◽  
Global Mapping

Secretory pathway genes assessed by high-throughput microscopy and synthetic genetic array analysis

2011 ◽  
Vol 7 (9) ◽  
pp. 2589 ◽  
Author(s):  
Peter W. Bircham ◽  
David R. Maass ◽  
Christina A. Roberts ◽  
Poh Y. Kiew ◽  
Yee S. Low ◽  
...  
Keyword(s):  
High Throughput ◽  
Secretory Pathway ◽  
Synthetic Genetic Array ◽  
Array Analysis
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