scholarly journals Sequencing and phylogenetic analysis of the stn gene of Salmonella species isolated from different environmental sources at Lake Qarun protectorate: The role of migratory birds and public health importance

2021 ◽  
pp. 2764-2772
Author(s):  
Hanan S. Khalefa ◽  
Zeinab S. Ahmed ◽  
Fatma Abdel-Kader ◽  
Eman M. Ismail ◽  
Esraa A. Elshafiee
Keyword(s):  
Public Health ◽  
Phylogenetic Analysis ◽  
Water Samples ◽  
Brine Shrimp ◽  
Chicken Manure ◽  
Farm Animals ◽  
Aquatic Bird ◽  
Aquatic Birds ◽  
Fulica Atra ◽  
Lake Qarun

Background and Aim: Salmonella causes most foodborne bacterial illnesses worldwide. It is found in various hosts, including pets, farm animals, and wild animals, as well as the environment. This study aimed to examine the epidemiological relationship between Salmonella isolates from aquatic environments and those from other avian hosts. Materials and Methods: The study examined 12 water samples, 210 aquatic animals, and 45 migratory aquatic bird samples collected from the protected area of Lake Qarun in El-Fayoum Governorate, Egypt, during migration seasons from different waterfowl migration areas (from October 2018 to January 2019). In addition, 45 fecal samples from domestic chickens were collected from the same geographic location from poultry farms. Bacteriological examination and polymerase chain reaction assay of two virulence genes (i.e., invA and stn) were performed to isolate and identify Salmonella. Results: Salmonella was isolated from 58.3% (7/12) of Lake Qarun water samples, 13.3% (6/45) of migratory waterfowl, 6.6% of (3/45) of chickens (Gallus gallus domesticus), and 4.3% (3/70) of fish and pooled brine shrimp. In migratory aquatic bird species that were sampled, Salmonella were isolated from 23.1% (3/13) of Eurasian coot (Fulica atra), 12.5%, (1/8) of green-winged teal (Anas cardolinesis), 10% (2/20) of northern shoveler (Spatula clypeata), and 0% (0/4) of mallard duck (Anas platyrhynchos). In 35 Tilapia, Salmonella was isolated by (8.6%) 5.7% of external surfaces, 2.85% from the intestine, and 0% from the muscle. No Salmonella was isolated from the 175 brine shrimp samples. Phylogenetic analysis using the stn genes of Salmonella isolated from the aquatic environment, migratory aquatic birds, and chicken showed a strong association between these isolates. In addition, a higher nucleotide identity percentage was observed between the sequences recovered from migratory aquatic birds and Lake Qarun water samples. Conclusion: Salmonella distribution was confirmed through migratory aquatic birds, based on our phylogeny tree analysis, Salmonella considered a likely carrier of zoonotic bacterial pathogens. Furthermore, the close relationship between chicken and fish sequences highlights the scenarios of using chicken manure in fish farms and its public health implications. The presence of Salmonella in different environmental sources spotlights the urgent need to control and break down its epidemiological cycle.

scholarly journals Detection of assemblages A and B of Giardia duodenalis in water and sewage from São Paulo state, Brazil

2011 ◽  
Vol 9 (2) ◽  
pp. 361-367 ◽  
Author(s):  
Licia Natal Fernandes ◽  
Patrícia Pereira de Souza ◽  
Ronalda Silva de Araújo ◽  
Maria Tereza Pepe Razzolini ◽  
Rodrigo Martins Soares ◽  
...  
Keyword(s):  
Public Health ◽  
Phylogenetic Analysis ◽  
Water Samples ◽  
Nested Pcr ◽  
Giardia Duodenalis ◽  
Spring Water ◽  
Human Faeces ◽  
Paulo State ◽  
Treated Sewage ◽  
Assemblage B

Giardia duodenalis is a protozoan that parasitizes humans and other mammals and causes giardiasis. Although its isolates have been divided into seven assemblages, named A to G, only A and B have been detected in human faeces. Assemblage A isolates are commonly divided into two genotypes, AI and AII. Even though information about the presence of this protozoan in water and sewage is available in Brazil, it is important to verify the distribution of different assemblages that might be present, which can only be done by genotyping techniques. A total of 24 raw and treated sewage, surface and spring water samples were collected, concentrated and purified. DNA was extracted, and a nested PCR was used to amplify an 890 bp fragment of the gdh gene of G. duodenalis, which codes for glutamate dehydrogenase. Positive samples were cloned and sequenced. Ten out of 24 (41.6%) samples were confirmed to be positive for G. duodenalis by sequencing. Phylogenetic analysis grouped most sequences with G. duodenalis genotype AII from GenBank. Only two raw sewage samples presented sequences assigned to assemblage B. In one of these samples genotype AII was also detected. As these assemblages/genotypes are commonly associated to human giardiasis, the contact with these matrices represents risk for public health.

scholarly journals Radon Activity Concentrations in Natural Hot Spring Water: Dose Assessment and Health Perspective

2021 ◽  
Vol 18 (3) ◽  
pp. 920
Author(s):  
Eka Djatnika Nugraha ◽  
Masahiro Hosoda ◽  
June Mellawati ◽  
Untara Untara ◽  
Ilsa Rosianna ◽  
...  
Keyword(s):  
Public Health ◽  
Effective Dose ◽  
Radiation Protection ◽  
Water Samples ◽  
Hot Springs ◽  
Hot Spring ◽  
Annual Effective Dose ◽  
Spring Water ◽  
Dose Assessment ◽  
West Java

The world community has long used natural hot springs for tourist and medicinal purposes. In Indonesia, the province of West Java, which is naturally surrounded by volcanoes, is the main destination for hot spring tourism. This paper is the first report on radon measurements in tourism natural hot spring water in Indonesia as part of radiation protection for public health. The purpose of this paper is to study the contribution of radon doses from natural hot spring water and thereby facilitate radiation protection for public health. A total of 18 water samples were measured with an electrostatic collection type radon monitor (RAD7, Durridge Co., USA). The concentration of radon in natural hot spring water samples in the West Java region, Indonesia ranges from 0.26 to 31 Bq L−1. An estimate of the annual effective dose in the natural hot spring water area ranges from 0.51 to 0.71 mSv with a mean of 0.60 mSv for workers. Meanwhile, the annual effective dose for the public ranges from 0.10 to 0.14 mSv with an average of 0.12 mSv. This value is within the range of the average committed effective dose from inhalation and terrestrial radiation for the general public, 1.7 mSv annually.

scholarly journals South-east Asian Zika virus strain linked to cluster of cases in Singapore, August 2016

2016 ◽  
Vol 21 (38) ◽  
Author(s):  
Sebastian Maurer-Stroh ◽  
Tze-Minn Mak ◽  
Yi-Kai Ng ◽  
Shiau-Pheng Phuah ◽  
Roland G Huber ◽  
...  
Keyword(s):  
Public Health ◽  
Phylogenetic Analysis ◽  
Virus Strain ◽  
Zika Virus ◽  
East Asian ◽  
Public Health Emergency ◽  
Global Public Health ◽  
Genome Sequences ◽  
First Case ◽  
Large Outbreak

Zika virus (ZIKV) is an ongoing global public health emergency with 70 countries and territories reporting evidence of ZIKV transmission since 2015. On 27 August 2016, Singapore reported its first case of local ZIKV transmission and identified an ongoing cluster. Here, we report the genome sequences of ZIKV strains from two cases and find through phylogenetic analysis that these strains form an earlier branch distinct from the recent large outbreak in the Americas.

scholarly journals Investigation of Cryptosporidium spp. and Giardia spp. in Lake Eğirdir (Isparta)

2021 ◽  
Author(s):  
Tuğba Sağlam ◽  
Serdar Düşen ◽  
Meral Apaydın Yağcı ◽  
Abdülkadir Yağcı
Keyword(s):  
Public Health ◽  
Drinking Water ◽  
Water Samples ◽  
Average Density ◽  
Agricultural Irrigation ◽  
Control Programs ◽  
Cryptosporidium Spp ◽  
Direct Microscopic Examination ◽  
Different Seasons ◽  
Autumn And Winter

Objective: The aim of this study was to assess both the presence and seasonal variability of Cryptosporidium spp. and Giardia spp. in Eğirdir Lake within the borders of Isparta province, which is used for drinking, agricultural irrigation and recreational purposes. Method: The research was carried out between July 2016 and January 2017 and water samples were taken from five different stations in three different seasons in Lake Eğirdir. After direct microscopic examination of the samples (Native-Lugol method), they were stained with Modified Acid Fast (MAF), and examined under the light microscope for parasites. Results: Cryptosporidium spp and Giardia spp were detected in 15 water samples in summer months, with an average density of 99.2% and 93.3% respectively, in Lake Eğirdir. In addition, both parasites were also detected intensively in autumn and winter Conclusion: The use of Lake Eğirdir for daily needs of people, agriculture andrecreational purposes cause increase in protozoal density. Thus, it is necessary to conduct parasitological studies on Lake Eğirdir, especially during the periods of swimming tourism, to determine the protozoal epidemiology in humans and animals. In addition, it is important to carry out adequate disinfection processes and plan the necessary control programs in terms of public health in the regions where Lake Eğirdir is used as drinking water.

scholarly journals Increasing Recombinant Strains Emerged in Norovirus Outbreaks in Jiangsu, China: 2015–2018

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jianguang Fu ◽  
Changjun Bao ◽  
Xiang Huo ◽  
Jianli Hu ◽  
Chao Shi ◽  
...  
Keyword(s):  
Public Health ◽  
Phylogenetic Analysis ◽  
Primary Schools ◽  
Point Mutations ◽  
The Other ◽  
Recombinant Strains ◽  
Viral Rdrp ◽  
Conformational Epitopes ◽  
Orf2 Region ◽  
Recombination Breakpoints

AbstractFrom January 2015 to December 2018, 213 norovirus outbreaks with 3,951 patients were reported in Jiangsu, China. Based on viral RdRp and VP1 genes, eight genotypes, GII.2[P16] (144, 67.6%), GII.3[P12] (21, 9.9%), GII.6[P7] (5, 2.3%), GII.14[P7] (4, 1.9%), GII.4 Sydney[P31] (3, 1.4%), GII.1[P33] (1, 0.5%), GII.2[P2] (3, 1.4%), and GII.17[P17] (16, 7.5%) were identified throughout the study period. These genotypes were further regrouped as GII.R (Recombinant) and GII.Non-R (Non-recombinant) strains. In this report we showed that GII.R strains were responsible for at least 178 (83.6%) of 213 norovirus-positive outbreaks with a peak in 2017 and 2018. Most norovirus outbreaks occurred in primary schools and 94 of 109 (86.2%) outbreaks in primary schools were caused by GII.R, while GII.Non-R and GII.NT (not typed) strains accounted for 6 (5.5%) and 9 (8.3%) norovirus outbreaks, respectively. The SimPlot analysis showed recombination breakpoints near the ORF1/2 junction for all six recombinant strains. The recombination breakpoints were detected at positions varying from nucleotides 5009 to 5111, localized in the ORF1 region for four strains (GII.2[P16], GII.3[P12], GII.6[P7], and GII.14[P7]) and in the ORF2 region for the other (GII.4 Sydney[P31] and GII.1[P33]). We identified four clusters, Cluster I through IV, in the GII.P7 RdRp gene by phylogenetic analysis and the GII.14[P7] variants reported here belonged to Cluster IV in the RdRp tree. The HBGA binding site of all known GII.14 strains remained conserved with several point mutations found in the predicted conformational epitopes. In conclusion, gastroenteritis outbreaks caused by noroviruses increased rapidly in the last years and these viruses were classified into eight genotypes. Emerging recombinant noroviral strains have become a major concern and challenge to public health.

scholarly journals Utility of a PCR-based method for rapid and specific detection of toxigenic Microcystis spp. in farm ponds

2020 ◽  
Vol 32 (3) ◽  
pp. 369-381 ◽  
Author(s):  
Jian Yuan ◽  
Hyun-Joong Kim ◽  
Christopher T. Filstrup ◽  
Baoqing Guo ◽  
Paula Imerman ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Water Samples ◽  
Low Cost ◽  
Farm Animals ◽  
Rrna Gene ◽  
Analytical Sensitivity ◽  
Water Safety ◽  
Farm Ponds ◽  
Pcr Method

Microcystis is a widespread freshwater cyanobacterium that can produce microcystin, a potent hepatotoxin harmful to animals and humans. Therefore, it is crucial to monitor for the presence of toxigenic Microcystis spp. to provide early warning of potential microcystin contamination. Microscopy, which has been used traditionally to identify Microcystis spp., cannot differentiate toxigenic from non-toxigenic Microcystis. We developed a PCR-based method to detect toxigenic Microcystis spp. based on detection of the microcystin synthetase C ( mcyC) gene and 16S rRNA gene. Specificity was validated against toxic and nontoxic M. aeruginosa strains, as well as 4 intergeneric freshwater cyanobacterial strains. Analytical sensitivity was as low as 747 fg/µL genomic DNA (or 3 cells/µL) for toxic M. aeruginosa. Furthermore, we tested 60 water samples from 4 farm ponds providing drinking water to swine facilities in the midwestern United States using this method. Although all water samples were positive for Microcystis spp. (i.e., 16S rRNA gene), toxigenic Microcystis spp. were detected in only 34 samples (57%). Seventeen water samples contained microcystin (0.1–9.1 μg/L) determined with liquid chromatography–mass spectrometry, of which 14 samples (82%) were positive for mcyC. A significant correlation was found between the presence of toxigenic Microcystis spp. and microcystin in water samples ( p = 0.0004). Our PCR method can be a low-cost molecular tool for rapid and specific identification of toxigenic Microcystis spp. in farm ponds, improving detection of microcystin contamination, and ensuring water safety for farm animals.

scholarly journals Assessing Potable Water Quality and Identifying Areas of Waterborne Diarrheal and Fluorosis Health Risks Using Spatial Interpolation in Peshawar, Pakistan

Water ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2163
Author(s):  
Mahmood Ahmad ◽  
Arshad Jamal ◽  
Xiao-Wei Tang ◽  
Mohammed A. Al-Sughaiyer ◽  
Hassan M. Al-Ahmadi ◽  
...  
Keyword(s):  
Public Health ◽  
Water Quality ◽  
Spatial Distribution ◽  
Water Samples ◽  
Potable Water ◽  
World Health ◽  
Faecal Coliforms ◽  
Entire Study ◽  
Distribution Mapping ◽  
Potable Water Quality

Waterborne diseases have become one of the major public health concerns worldwide. This study is aimed to investigate and develop spatial distribution mapping of the potable water quality parameters in the city of Peshawar, Pakistan. A total of 108 water samples collected across the entire study area were subjected to physio-chemical and biological analyses. Tested parameters included pH, turbidity, temperature, fluoride concentration levels, and bacterial counts (faecal coliforms). Inverse distance weighting (IDW) interpolation in geographic information systems (GIS) was used for spatial analysis. Test results revealed that 48% of water samples had faecal coliforms count (per 100 mL) greater than World Health Organization (WHO) minimum limits, while 31% of samples had fluoride concentrations in excess of the WHO maximum guide values. Spatial distribution mapping was developed for faecal coliforms count and fluoride ion concentration using ArcGIS to highlight the high-risk settlements in the study area. Results showed that around 20% area under faecal coliforms and approximately 33% area based on fluoride concentrations fall under the need for treatment category. The pH and turbidity were found in compliance with WHO desirable limits. The sanitary inspection score significantly depicted that ineffective multi-barrier approaches consequently deteriorated the water quality at the consumer’s end. Findings from the present study shall be useful to policymakers for adopting necessary remedial measures before it severely affects public health.

Study of a hepatitis E virus outbreak involving drinking water and sewage contamination in Shimla, India, 2015–2016

2019 ◽  
Vol 113 (12) ◽  
pp. 789-796 ◽  
Author(s):  
Anuradha S Tripathy ◽  
Meenal Sharma ◽  
Avinash R Deoshatwar ◽  
Prasad Babar ◽  
Rakesh Bharadwaj ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Water Samples ◽  
Hepatitis E Virus ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Hepatitis E ◽  
Rt Pcr ◽  
Oral Transmission ◽  
Sewage Contamination ◽  
Current Outbreak

Abstract Background Hepatitis E, caused by hepatitis E virus (HEV), accounts for 50% of acute hepatitis cases in India. We report an outbreak of hepatitis E in Shimla, India, in 2015–2016. Methods ICMR-National Institute of Virology (NIV), Pune, received two batches of water samples from Shimla in January 2016 to test for the presence of enterically transmitted hepatitis viruses. Subsequently, 57 icterus patients were tested for various markers of hepatotropic viruses, i.e. anti-HEV IgM/IgG, anti-hepatitis A virus (anti-HAV) IgM/IgG antibodies and HEV RNA. Water samples were screened for HEV and HAV RNA followed by phylogenetic analysis. Results Overall, 48/57 patients availing municipal water had evidence of HEV infection, detected by serology and RT-PCR. All the water samples tested positive for HEV and HAV RNA, while the patients were negative for anti-HAV IgM antibody, indicating no recent HAV infection. Phylogenetic analysis confirmed the aetiological agent of the current outbreak to be HEV genotype 1. Conclusions Serology and RT-PCR confirmed HEV as the aetiology of the outbreak. The absence of new cases of hepatitis A, despite the presence of HAV in the water supply, could be due to previously acquired immunity. Sewage contamination of water leading to faecal-oral transmission of HEV still remains a concern, thus emphasising the need for a vaccination/control strategy.

scholarly journals Microbiological Analysis of Drinking Water of Kathmandu Valley

1970 ◽  
Vol 5 (5) ◽  
pp. 112-114 ◽  
Author(s):  
Tista Prasai ◽  
Binod Lekhak ◽  
Dev Raj Joshi ◽  
Madhav Prasad Baral
Keyword(s):  
Public Health ◽  
Drinking Water ◽  
Water Samples ◽  
Enteric Bacteria ◽  
Coliform Bacteria ◽  
Kathmandu Valley ◽  
Microbiological Analysis ◽  
Public Health Importance ◽  
Guideline Value ◽  
Drinking Water Samples

Drinking water quality assessment in Kathmandu valley has always been crucial with reference to public health importance. A study was conducted to evaluate the quality of drinking water of the valley. A total of 132 drinking water samples were randomly collected from 49 tube wells, 57 wells, 17 taps and 9 stone spouts in different places of Kathmandu valley. The samples were analyzed for microbiological parameters. Total plate and coliform count revealed that 82.6% and 92.4% of drinking water samples found to cross the WHO guideline value for drinking water. During the study, 238 isolates of enteric bacteria were identified, of which 26.4% were Escherichia coli, 25.6% were Enterobacter spp, 23% were Citrobacter spp, 6.3% were Pseudomonas aeruginosa, 5.4% were Klebsiella spp, 4.0% were Shigella spp, 3.0% were Salmonella typhi, 3.0% were Proteus vulgaris, 3.0% were Serratia spp and 1.0% were Vibrio cholerae . Key words: Drinking water; Public health; Coliform; Bacteria. DOI: 10.3126/sw.v5i5.2667 Scientific World, Vol. 5, No. 5, July 2007 112-114

scholarly journals Emergence of influenza A viruses

2001 ◽  
Vol 356 (1416) ◽  
pp. 1817-1828 ◽  
Author(s):  
R. J. Webby ◽  
R. G. Webster
Keyword(s):  
Intermediate Host ◽  
Influenza A ◽  
Influenza Viruses ◽  
Human Populations ◽  
Aquatic Bird ◽  
Intermediate Hosts ◽  
Aquatic Birds ◽  
Influenza A Viruses ◽  
Bird Populations ◽  
Human Viruses

Pandemic influenza in humans is a zoonotic disease caused by the transfer of influenza A viruses or virus gene segments from animal reservoirs. Influenza A viruses have been isolated from avian and mammalian hosts, although the primary reservoirs are the aquatic bird populations of the world. In the aquatic birds, influenza is asymptomatic, and the viruses are in evolutionary stasis. The aquatic bird viruses do not replicate well in humans, and these viruses need to reassort or adapt in an intermediate host before they emerge in human populations. Pigs can serve as a host for avian and human viruses and are logical candidates for the role of intermediate host. The transmission of avian H5N1 and H9N2 viruses directly to humans during the late 1990s showed that land-based poultry also can serve between aquatic birds and humans as intermediate hosts of influenza viruses. That these transmission events took place in Hong Kong and China adds further support to the hypothesis that Asia is an epicentre for influenza and stresses the importance of surveillance of pigs and live-bird markets in this area.

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