Proliferative activity of the epithelial sheet of the mucous membrane in the lower airways during experimental diabetes mellitus

2013 ◽  
Vol 59 (3) ◽  
pp. 27-29
Author(s):  
O A Pivovarova ◽  
B N Man'kovskiĭ
Keyword(s):  
Diabetes Mellitus ◽  
Experimental Diabetes ◽  
Intraperitoneal Administration ◽  
Male Rats ◽  
Control Group ◽  
Intact Male ◽  
Buffer Solution ◽  
Solution Ph ◽  
Citrate Buffer ◽  
Epithelial Sheet

The present study was designed to develop the experimental model of diabetes mellitus based on 5-6 month-old Wistar rats weighing 234.00±2.64 g (n=47). Diabetes was induced by a single intraperitoneal administration of streptozotocin (60 mg/kg, "Sigma", USA) in a 0.1 M citrate buffer solution, pH 4.5. The control group was comprised of 43 intact male rats. The animals with experimental diabetes had a reduced number of secretory cell nuclei per unit area of the epithelial sheet of the bronchial tree; the area of epitheliocyte nuclei also decreased.

scholarly journals Comparative characteristics of the manifestations of damage and reparative processes in the mucous membrane of the duodenum of rats under the conditions of skin burns and skin burns associated with diabetes

2020 ◽  
Vol 26 (2) ◽  
pp. 39-44
Author(s):  
I.A. Tymoshenko ◽  
L.M. Sokurenko ◽  
A.Ya. Yanchyshyn ◽  
V.A. Pastukhova
Keyword(s):  
Diabetes Mellitus ◽  
Mucous Membrane ◽  
Structural Changes ◽  
Burn Injury ◽  
Experimental Diabetes ◽  
Male Rats ◽  
Control Group ◽  
Experimental Diabetes Mellitus ◽  
Buffer Solution ◽  
Solution Ph

Currently, severe thermal injury is becoming one of the most important problems of practical medicine. Diabetes is also recognized as another global medical and social challenge of our century. The emergency situation for the treatment and prevention of the consequences of these pathologies is a consequence of the lack of a reliable theoretical basis for solving specific clinical problems regarding the course of burns, diabetes and their complications. The aim of the study is to establish the patterns of structural changes in the mucous membrane of the duodenum after burn injury of the skin of rats under conditions of experimental diabetes mellitus. The study was performed on 63 laboratory white adult male rats weighing 180-210 g, which were divided into 3 groups: intact animals, rats with skin burns and rats with skin burns on the background of diabetes. The model of experimental diabetes mellitus was reproduced by administering Streptozotocin to rats intraperitoneally once at a dose of 50 mg/kg, pre-dissolved in 0.1 M citrate buffer solution (pH=4.5). The control of the development of hyperglycemia in the experimental groups was the level of glucose in the blood 24.24±0.79 mmol/l. In the control group this index was 8.03±0.4 mmol/l. Rats with skin burns revealed destructive manifestations, which are accompanied by an active inflammatory reaction and corresponding necrotic changes, while rats with skin burns on the background of diabetes mellitus pathological processes are not just “summed up”, but in some way adaptively modified with the involvement of stress mechanisms of the endoplasmic reticulum and associated autophagy.

scholarly journals Age features of morphological changes of vessels of testicular hemomicrocirculatory flow in streptozotocin diabetes mellitus

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Ivan-Andriy Kondrat
Keyword(s):  
Diabetes Mellitus ◽  
Morphological Changes ◽  
Glycosylated Hemoglobin ◽  
Streptozotocin Diabetes ◽  
Male Rats ◽  
Diabetic Microangiopathy ◽  
Control Group ◽  
Buffer Solution ◽  
Statistical Research ◽  
Citrate Buffer

Medico-social problem of diabetes is caused by early disability and mortality of patients due to specific complications of micro- and macroangiopathies. Therefore, the aim of our study was to establish morphological changes in vessels of the hemomicrocirculatory flow of the testes of immature rats with experimental streptozotocin diabetes mellitus (SDM). The material for the study were the testicles of 20 two-month-old immature (weighing 65-95 g) white outbred male rats, which were divided equally into 2 groups: experimental and control ones. SDM in animals of the experimental group was simulated by a single intraperitoneal injection of streptozotocin (dissolved in 0.1 M citrate buffer solution with a pH of 4.5) at a dose of 7 mg per 100 g of mass. The control group of animals received intraperitoneally an equivalent dose of 0.1 M citrate buffer. Histological, electron microscopic, biochemical, morphometric and statistical research methods were used. It was found that in the early stages of SDM (14th day) on the background of hyperglycemia in the hemomicrocirculatory flow of the testes there is a spasm of the vessels of the afferent link, which is confirmed by a decrease in the area of arterioles lumen and an increase in their VI. On the 56th day of SDM, on the background of elevated levels of glucose and glycosylated hemoglobin in the links of the hemorrhagic circulatory flow of the testes there are initial signs of diabetic microangiopathy, manifested by: hemorheological disorders in micro-hemo-vessels (erythrocyte sludges, adhesion of erythrocytes and platelets, microclasmatosis), decreased capacity of arterioles and capillaries (increase in VI, respectively by 1.2 and 1.9 times), microclasmatosis, thickening and proliferation of the basement membrane of capillaries. Thus, on the 56th day of SDM in the hemomicrocirculatory flow of the testes, the development of diabetic microangiopathy is observed, which leads to the disruption of the blood-testis barrier, and as a consequence, to a violation of spermatogenesis.

scholarly journals Pathogenetical aspects of tubulointerstitial syndrome development in alloxan-induced experimental diabetes mellitus

2021 ◽  
Vol 25 (1) ◽  
pp. 17-21
Author(s):  
О. А. Olenovych
Keyword(s):  
Diabetes Mellitus ◽  
Filtration Rate ◽  
Experimental Diabetes ◽  
Intraperitoneal Administration ◽  
Water Extract ◽  
Male Rats ◽  
Control Group ◽  
Sodium Reabsorption ◽  
Experimental Diabetes Mellitus ◽  
Sodium And Potassium

Annotation. The aim of our study was to explore the pathogenetical aspects of tubulointerstitial syndrome development in alloxan-induced experimental diabetes mellitus. The experiments were carried out on 20 white non-linear mature male rats, 10 with experimental diabetes mellitus (EDМ) induced by intraperitoneal administration of alloxan at a dose of 160 mg/kg of body weight, 10 intact rats served as the control group. 25 days after administration of the diabetogenic substance, the animals were withdrawn from the experiment. The concentration of sodium and potassium ions in urine and blood plasma samples was determined, followed by calculation (considering water-induced 2-hour diuresis and endogenous creatinine clearance) of glomerular filtration rate, electrolyte excretion, their filtration rate, absolute and relative reabsorption, clearance, their proximal and distal renal transport. Removed after decapitation rats’ kidneys were dissected to 3 parts – renal cortex, medulla and papilla, sodium and potassium content was determined in water-extract of the corresponding part of the renal parenchyma, and papillary-cortical, papillary-medullar and medullary-cortical concentration ion gradients were calculated. Significant suppression of papillary-medullar and papillary-cortical concentration sodium gradients, as well as a slight limitation of its medullary-cortical gradient were established. The concentration potassium gradients were significantly reduced. Statistical processing of the obtained data was carried out with the determination of the average value, standard deviations. To assess the probability of the difference between the study groups used non-parametric Mann-Whitney ranking criterion according to the algorithms implemented in the computer program “Statistica for Windows”, “Version 8.0”. There was a decrease of the sodium-potassium ratio in urine, enhanced urinary excretion of potassium and an increase of its content in urine, as well as intensification of absolute transtubular sodium transport due to equivalent augmentation of the filtration charge of this cation, increase of proximal sodium reabsorption and, to a lesser degree, – of distal one. The distal and proximal sodium reabsorption, reduced to a unit of active nephrons, was found to be decreased, and the relative reabsorption of the cation significantly exceeded the control values, contributing to the limitation of natriuresis. The results of the study suggest that in 26-day alloxan-induced experimental diabetes hemodynamic-hyperperfusion overload on the tubular apparatus of the kidney causes the development of relative insufficiency of the proximal and distal tubules, disorders of hormone-dependent reabsorption of cations, limitation of regulatory influence of aldosterone and ADH with further tubulointerstitial disturbances that unable adequate osmotic concentration of urine.

scholarly journals APOPTOTIC PROCESSES’ RESEARCH ON THE MODEL OF TYPE 2 DIABETES MELLITUS ON OBESITY’S BACKGROUND UNDER THE INFLUENCE OF DENSE BEAN EXTRACT

2020 ◽  
Vol 73 (8) ◽  
pp. 1690-1695
Author(s):  
Viktoria A. Rybak ◽  
Viktoria V. Korol ◽  
Natalia V. Derkach
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Cell Function ◽  
Male Rats ◽  
Control Group ◽  
Citrate Buffer ◽  
Pancreatic Cell ◽  
Alcoholic Fatty Liver

The aim of the study was to observe the influence of dense bean extract on the intensity of apoptotic processes in the liver cells and pancreas of rats on a model of type 2 diabetes mellitus on obesity’s background. Materials and methods: The main method was to model type 2 diabetes mellitus on the background of obesity in organism of mature six-month-old male rats of the Wistar population (n = 21), weighing 150-170 g. The modelling was carried out by intraperitoneal low dose administration of streptozotocin (30 mg / kg, in citrate buffer pH = 4, 5) inside after three months period of keeping animals on a combined diet. Apoptosis in DNA samples of liver and pancreas cells was identified in duplicates using electrophoresis in a 1% agarose gel with using a 1kb DNA SibEnzyme apoptosis marker (from 10,000 to 250 nucleotides). Results: Only in two of the seven studied DNA samples of the pancreas of a group of rats, treated with a dense bean extract, were observed the traces of necrosis without detectable manifestations of the apoptotic process. It situates at the level of indicators of the animals’ intact control group and indicates the distinct effect’s presence which includes maintaining pancreas cells survival (in both endocrine and exocrine parts) if imbalance of carbohydrate and lipid metabolism take place in organism. Conclusion: Dense bean extract showed a more distinct effect than the comparison drug metformin in relation to the risk of premature loss of pancreatic cell function and the development of non-alcoholic fatty liver disease. A dense bean extract is promising for further pharmacological studies, with the aim of creating phytopreparations – «Glyphasonorm» tablets and «Glyfasolin» capsules for the correction of type 2 diabetes mellitus and its complications.

KIDNEY FUNCTION IN EXPERIMENTAL DIABETES

2019 ◽  
Vol 23 (1) ◽  
pp. 79-83
Author(s):  
A. Yu. Zharikov ◽  
B. A. Balandovich ◽  
R. O. Shchekochikhina ◽  
G. V. Zharikova
Keyword(s):  
Diabetes Mellitus ◽  
Renal Function ◽  
Disease Control ◽  
Experimental Diabetes ◽  
Intraperitoneal Administration ◽  
Urine Volume ◽  
Control Group ◽  
Excretory Function ◽  
Daily Urine ◽  
Intact Rats

THE AIM. To study the renal function of rats in conditions of streptozotocin-induced model of experimental diabetes mellitus. MATERIAL AND METHODS. The study was conducted on 25 male Wistar stock rats (12 individuals – a group of intact rats and 13 individuals – a disease control group). Diabetes mellitus was simulated by intraperitoneal administration of Stretpozotocin at a dose of 65 mg / kg. For a more selective simulation of type II diabetes, rats of the disease control group were injected intraperitoneally with a cytoflavin solution at the rate of 115 mg / kg nicotinamide dosage. In both groups, the daily urine volume was collected every 7 days during the month, in which the concentrations of glucose, protein, and creatinine were determined. Taking into account the daily volume of diuresis, excretion of glucose, protein, and creatinine was calculated. RESULTS. At baseline, all determinable indicators of renal function in rats did not differ between groups. Then, in the disease control group, there was a significant increase in daily diuresis, protein excretion, glucose excretion, and creatinine. As a result, by the end of the experiment, the magnitude of these indicators exceeded the level of intact rats by 2.0; 1.5, 3.2 and 1.9 times, respectively, CONCLUSION. Under conditions of the streptozotocin model of diabetes mellitus, characteristic changes in the renal excretory function are observed: an increase in daily diuresis, an increase in the excretion of glucose, an excretion of protein and creatinine.

Effects of oestradiol benzoate (OeB) and human chorionic gonadotrophin (hCG) on the testes and accessory sex glands of the rat

1981 ◽  
Vol 96 (2) ◽  
pp. 273-280 ◽  
Author(s):  
Mridula Chowdhury ◽  
Robert Tcholakian ◽  
Emil Steinberger
Keyword(s):  
Treated Group ◽  
Inhibitory Effect ◽  
Male Rats ◽  
Plasma Testosterone ◽  
Control Group ◽  
Intact Male ◽  
Intact Control ◽  
Testosterone Levels ◽  
Oestradiol Benzoate ◽  
Direct Inhibitory Effect

Abstract. It has been suggested that treatment of intact male rats with oestradiol benzoate (OeB) causes an interference with testosterone (T) production by the testes by a direct inhibitory effect on steroidogenesis. To test this hypothesis, different doses (5, 10 or 25 IU) of hCG were administered concomitantly with 50 μg of OeB to adult intact or hypophysectomized male rats. The testicular and plasma testosterone, and serum hCG levels were determined. The sex accessory weights were recorded. In the intact OeB-treated group of animals, hCG stimulated both the secondary sex organs and plasma testosterone levels above the intact control group. However, in hypophysectomized animals, although plasma testosterone levels increased above that of intact controls, their secondary sex organ weights did not. Moreover, inspite of high circulating hCG levels, the testicular testosterone content and concentration remained suppressed in OeB-treated animals. The reason for such dichotomy of hCG action on OeB-treated animals is not clear at present.

scholarly journals Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen receptor in formalin-fixed paraffin sections.

1993 ◽  
Vol 41 (11) ◽  
pp. 1599-1604 ◽  
Author(s):  
S R Shi ◽  
B Chaiwun ◽  
L Young ◽  
R J Cote ◽  
C R Taylor
Keyword(s):  
Androgen Receptor ◽  
Enzymatic Digestion ◽  
Antigen Retrieval ◽  
Urea Solution ◽  
Paraffin Sections ◽  
Buffer Solution ◽  
Solution Ph ◽  
Citrate Buffer ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed

We developed a staining protocol for demonstration of androgen receptor (AR) in formalin-fixed, paraffin-embedded tissue sections. The method is based on the antigen retrieval microwave (MW) heating technique. Results are compared with different types of enzyme digestion pre-treatments. The strongest immunostaining signal and clearest background were obtained by MW heating of dewaxed paraffin sections in 5% urea or citrate buffer solution (pH 6); pure distilled water gave less consistent results. Enzymatic digestion with pepsin (0.05% in 2 N HCl) for 30 min at room temperature, or trypsin followed by pronase, or pronase digestion alone, also produced enhanced staining of AR in some cases, but there was more nonspecific background, and specific reactivity was less intense. The antigen retrieval MW method can be used to demonstrate AR epitope in prostate tissue after fixation in formalin for as long as 7 days. AR immunolocalization was also compared in frozen and paraffin sections processed from the same specimen of prostate carcinoma tissue and was found to be qualitatively and quantitatively similar. This study also provided new information concerning the basic principles of the antigen retrieval MW method that may be helpful in further development of this technique.

scholarly journals Change of energy supply oxidation indices changes in rats with type 2 diabetes mellitus and acetaminophen toxic lesions

2017 ◽  
Author(s):  
O. B. Furka
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Energy Supply ◽  
Intraperitoneal Administration ◽  
Liver Homogenate ◽  
Intragastric Administration ◽  
Starch Solution ◽  
Citrate Buffer

Introduction. Acetaminophen is an effective and safe drug for emergency usage, but there are contraindications for its usage. Diabetes mellitus is an endocrine disease, which caused by the absolute or relative deficiency of pancreas hormone (insulin).The aim of the study – to learn the change of energy supply oxidation indices in animals with type 2 diabetes mellitus and acetaminophen toxic lesions in time dynamics.Research Methods. We conducted two series of experiments. In the first series toxic lesion was caused by a single intragastric administration of acetaminophen suspension in 2 % starch solution to animals in a dose of 1250 mg/kg (1/2 LD50). In the second series the suspension of acetaminophen in 2 % starch solution in a dose of 55 mg/kg was given. Non-genetic form of experimental type 2 diabetes mellitus was modeled by a single intraperitoneal administration of streptozotocin solution in doses 65 mg/kg, which was diluted by citrate buffer (pH 4.5) with the previous intraperitoneal nicotinamide administration in doses of 230 mg/kg. Rats, which were given the same amount of solvent (citrate buffer pH 4.5), were used as the control group.Results and Discussion. Cytochrome oxidase and succinate dehydrogenase activity decreased in rat liver homogenate under the influence of acetaminophen with type 2 diabetes mellitus throughout the experiment.Conclusions. Acetaminophen administration to rats with type 2 diabetes mellitus causes significant violations of energy supply oxidation.

scholarly journals Effects of holothuria extract on pain behaviour and Fos like immunoreactivity (FLI) in formalin pain

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Che Badariah AA ◽  
Asma HA ◽  
Mohd Nizam H ◽  
Siti FA
Keyword(s):  
Phase 1 ◽  
Intraperitoneal Administration ◽  
Male Rats ◽  
Repeated Measure ◽  
Saline Control ◽  
Control Group ◽  
Formalin Injection ◽  
Intraplantar Injection ◽  
Pain Behaviour ◽  
Behaviour Score

Introduction: The aim of this study was to determine the effects of gamat extract on pain behaviour and Fos like immunoreactivity (FLI) expression in the ventral posterolateral thalamus using the acute pain model. Materials & Methods: Fourteen Sprague-Dawley male rats (220-300 gram) were given intraplantar injection of 0.05ml formalin (1%) followed by intraperitoneal administration of either 4 mg/kg gamat extracts (Holothuria spp.) or saline (control). Behavioural changes were observed and rats were sacrificed 2 hours post-formalin injection. Immunohistochemistry testing was done on the brain sections. FLI was examined using a light microscope attached to an image analyser. The behaviour and FLI data were analysed using repeated measure analysis of variance and independent t-test respectively. Significance level was taken as p<0.05. Results: The control group has significantly higher pain scores compared to holothuria group (F (1) =13.635, p=0.003). There was significant reduction in the pain behaviour score in the holothuria group when compared to the control group in phase 1 (t (14) =2.9, p=0.012) and most of the time from 15 to 60 minutes post-formalin injection (t (12) =3.535, p=0.004). There was a significant reduction (P<0.05) in the number of FLI on the contralateral aspect of the ventral posterolateral thalamic nucleus in the group that received 4mg/kg of holothuria extract (63  3.18) compared to control group (84   6.36). Conclusion: This study showed that administration of holothuria extract significantly suppressed the pain behaviour and reduced the number of FLI in formalin injected rats compared to control.

scholarly journals Diabetic Rats Present High Mean Platelet Count in the Presence of Oral Infections

2017 ◽  
Vol 28 (5) ◽  
pp. 548-551 ◽  
Author(s):  
Luciana Louzada Ferreira ◽  
João Eduardo Gomes Filho ◽  
Dóris Hissako Sumida ◽  
Suely Regina Mogami Bonfim ◽  
Gustavo Sivieri-Araújo ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Platelet Count ◽  
Periodontal Disease ◽  
Rat Model ◽  
Inflammatory Diseases ◽  
Diabetic Rats ◽  
Apical Periodontitis ◽  
Buffer Solution ◽  
Citrate Buffer ◽  
Oral Infections

Abstract Platelet count is associated with inflammatory diseases like diabetes mellitus (DM), which in turn, is related in a bidirectional manner with apical periodontitis and periodontal disease. The aim of this study was to evaluate the effects of apical periodontitis and/or periodontal disease on mean platelet count in a rat model of diabetes mellitus. Eighty Wistar rats were randomly divided into 8 groups (n=10): control (C), apical periodontitis (AP), periodontal disease (PD), apical periodontitis with periodontal disease (AP-PD), diabetes mellitus (DM), diabetes mellitus with apical periodontitis (DM-AP), diabetes mellitus with periodontal disease (DM-PD) and diabetes mellitus with apical periodontitis and periodontal disease (DM-AP-PD). Rats were anesthetized and DM was induced with a single dose of streptozotocin diluted in citrate buffer solution. After 6 days, the DM was confirmed. The animals were sedated and apical periodontitis was induced by dental exposure and periodontal disease was induced by periodontal ligature. After 30 days, animals were anesthetized and the blood was collected by cardiac puncture. Samples were processed and the mean platelet count was obtained. Data were tabulated and subjected to statistical analysis (p<0.05). Diabetic rats had higher mean glycemic levels compared with nondiabetic rats at 6 and 36 days after DM induction (p<0.05). The DM-PD and DM-PD-AP groups showed increased mean platelet count compared to control and AP groups (p<0.05). The periodontal disease alone or associated with apical periodontitis influence mean platelet count in a rat model of diabetes mellitus.

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