scholarly journals The comparison between the effect of Glycyrrhizae uralensis (Gan-Cao) and Montelukast on the expression of T-bet and GATA-3 genes in children with allergic asthma

2022 ◽  
Vol 67 (4) ◽  
pp. 306-312
Author(s):  
Zang Ping ◽  
Xue Jun ◽  
Wang Yan ◽  
Zhang Jun
Keyword(s):  
Allergic Asthma ◽  
Mononuclear Cells ◽  
Respiratory Infections ◽  
Statistical Tests ◽  
Chinese Herb ◽  
Root Extract ◽  
Expression Ratio ◽  
Peripheral Blood Mononuclear ◽  
Chemical Treatments ◽  
The Relationship

Pediatric allergic asthma is a chronic disease that affects the lungs and airways. If a child is exposed to certain stimulants such as pollen inhalation, colds, or respiratory infections, the lungs become inflamed and if left untreated can lead to dangerous asthma attacks. One of the most important treatments for this disease is the use of leukotriene modulators, such as montelukast. But recently, due to easier access, cheaper prices and fewer side effects, attention has shifted to non-chemical treatments. Gan-Cao (Glycyrrhizae uralensis), as traditional Chinese medicine, has been proved to have a good therapeutic effect on experimental allergic asthma. But its anti-asthma mechanism is currently unclear. Therefore, the study aimed the comparison between the effect of Gan-Cao and montelukast on the expression of T-bet and GATA-3 genes in children with allergic asthma. For this purpose, fifty children with allergic asthma were divided into two groups. The first group was treated with montelukast for one month. The second group was treated with Gan-Cao root extract. Then the peripheral blood mononuclear cells were isolated, their RNA was extracted, and the relative expression of T-bet and GATA3 transcription factors was evaluated by Real-time PCR. The relationship between them and risk factors for asthma was assessed by relevant statistical tests. The result showed the expression of the GATA3 gene (P = 0.102), T-bet gene (P = 0.888), and the expression ratio of T-bet/GATA-3 genes (P = 0.061) was not significantly different between the two groups. It showed that Gan-Cao can affect the expression of these genes just as much as montelukast. Therefore, this Chinese herb can be used as an alternative or supplement medicine to treat allergic asthma in children.

Vertical transmission of HCV is related to maternal peripheral blood mononuclear cell infection

Blood ◽  
2000 ◽  
Vol 96 (6) ◽  
pp. 2045-2048 ◽  
Author(s):  
Chiara Azzari ◽  
Massimo Resti ◽  
Maria Moriondo ◽  
Roberto Ferrari ◽  
Paolo Lionetti ◽  
...  
Keyword(s):  
Vertical Transmission ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Hcv Rna ◽  
Cell Infection ◽  
Transmitted Infection ◽  
Peripheral Blood Mononuclear ◽  
Maternal Peripheral Blood ◽  
Hcv Transmission ◽  
The Relationship

Abstract Infection of peripheral blood mononuclear cells (PBMNCs) has been demonstrated to be a crucial event in the vertical transmission of viruses, and it is known that hepatitis C virus (HCV) can infect PBMNCs. The relationship between vertical transmission of HCV and the presence of positive and negative strands of HCV-RNA in the PBMNCs of HCV-carrier mothers was investigated using reverse transcriptase–polymerase chain reaction (RT-PCR). During the study, 13 consecutive mothers who transmitted infection to their offspring and 53 consecutive mothers who did not were examined. The positive strand of HCV-RNA was identified in the PBMNCs of all mothers who transmitted the infection and in 13 of 53 mothers who did not (P < 10−6). The HCV-RNA−strand was found in 5 of 13 mothers who transmitted the infection, and the strand was not found in the mothers who did not transmit the infection (P = .0001). Neither maternal PBMNC infection nor HCV transmission to the offspring was significantly related to the viral genotype or to the maternal viral load. These data show that maternal PBMNC infection by HCV and viral replicative activity in PBMNCs are important factors in the transmission of HCV from mother to child. The mechanism through which HCV infection of PBMNC favors vertical transmission of the virus is still incompletely understood.

scholarly journals Circulating immunoglobulin-secreting cells in patients with plasma cell dyscrasia

Blood ◽  
1980 ◽  
Vol 55 (4) ◽  
pp. 590-594 ◽  
Author(s):  
K Shimizu ◽  
T Murate ◽  
A Kunii
Keyword(s):  
Plasma Cell ◽  
Mononuclear Cells ◽  
Clinical Stage ◽  
Laboratory Data ◽  
Plaque Assay ◽  
Plasma Cell Dyscrasia ◽  
Healthy Individuals ◽  
Normal Range ◽  
Peripheral Blood Mononuclear ◽  
The Relationship

Abstract Immunoglobulin-secreting cells (ISC) in peripheral blood mononuclear cells (PBM) isolated from patients with plasma cell dyscrasia of various stages were studied using reverse hemolytic plaque assay. Normal healthy individuals contained 35 +/- 16 ISC/10(5) PBM. Aleukemic or subleukemic patients with overt myeloma contained 825 +/- 713 ISC, whereas leukemic patients contained 12,675 +/- 2520 ISC. Patients of premyelomatous stage, however, contained ISC/10(5) PBM of normal range. The relationship between ISC and clinical stage or laboratory data is discussed.

scholarly journals The CD40 rs1883832 Polymorphism Affects Sepsis Susceptibility and sCD40L Levels

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Zuo-Liang Liu ◽  
Jing Hu ◽  
Xue-Fei Xiao ◽  
Yue Peng ◽  
Shang-Ping Zhao ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Healthy Controls ◽  
Peripheral Blood Mononuclear ◽  
Single Nucleotide ◽  
Protein Levels ◽  
Potential Association ◽  
Functional Single Nucleotide Polymorphism ◽  
The Relationship ◽  
Control Study ◽  
Increased Susceptibility

Sepsis is a severe and progressive disease characterized by systemic inflammatory response syndrome (SIRS). CD40 serves as a vital link between immune response and inflammation. This study was designed to investigate the potential association between a functional single-nucleotide polymorphism (SNP) of CD40 (rs1883832) and susceptibility to sepsis. We first performed a case-control study to explore the relationship between the CD40 rs1883832 polymorphism and sepsis. CD40 mRNA expression and protein expression were determined by real-time PCR and western blotting, respectively, in peripheral blood mononuclear cells (PBMCs) from sepsis patients and healthy controls. The plasma sCD40L levels in the two groups were measured by ELISA. The results showed that the frequencies of the TT genotype and the CD40 rs1883832 T allele were significantly higher in sepsis patients than in healthy controls. Plasma sCD40L levels were also significantly increased in sepsis patients. In addition, TT genotype carriers among sepsis patients displayed the highest CD40 expression at both the mRNA and protein levels, accompanied by the highest plasma sCD40L concentrations. In conclusion, the CD40 rs1883832 T allele acts as a risk factor for increased susceptibility to sepsis and may be involved in the process of sepsis through regulation of CD40 expression and plasma sCD40L levels.

scholarly journals Low-Dose Steroid Therapy Is Associated with Decreased IL-12 Production in PBMCs of Severe Septic Patients

2016 ◽  
Vol 2016 ◽  
pp. 1-8
Author(s):  
Huang-Pin Wu ◽  
Chi-Chung Shih ◽  
Duen-Yau Chuang ◽  
Tien-Hsing Chen
Keyword(s):  
Steroid Therapy ◽  
Low Dose ◽  
Mononuclear Cells ◽  
Human Leukocyte ◽  
Mortality Rates ◽  
Peripheral Blood Mononuclear ◽  
Leukocyte Antigen ◽  
Hla Dr ◽  
Relationship Of ◽  
The Relationship

Background.Sepsis-induced immunosuppression may result in higher mortality rates in patients.Methods. We examined the relationship of cytokine responses from stimulated peripheral blood mononuclear cells (PBMCs) and monocyte human leukocyte antigen-DR (HLA-DR) expression (days 1 and 7) with low-dose steroid therapy in 29 septic patients. Patients were treated according to the guidelines. Thirty healthy controls were enrolled for validation.Results. Eighteen patients were prescribed low-dose steroids and 11 were not. Interleukin- (IL-) 12 responses in patients without low-dose steroid therapy on days 1 and 7 were higher than those with low-dose steroid therapy. Compared to day 1, IL-12 responses significantly increased on day 7 in patients without low-dose steroid therapy. After regression analysis, the change in the IL-12 response from day 7 to day 1 was found to be independently associated with the low-dose steroid therapy. There was no difference in monocyte HLA-DR expression between patients treated with and without low-dose steroid on day 1 or 7. No change in monocyte HLA-DR expression from day 7 to day 1 was observed in patients with or without low-dose steroid therapy.Conclusion. Decreased IL-12 response was associated with the low-dose steroid therapy in PBMCs of septic patients.

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