scholarly journals Rеgаrding the standаrdіzаtion of glycine and thiotriazoline in the model mixture by high-performance liquid chromatography

Author(s):  
O. V. Khromylova ◽  
M. О. Avramenko ◽  
H. R. Nimenko ◽  
E. Yu. Hura
Author(s):  
O. V. Korobova ◽  
L. K. Samarska ◽  
S. L. Humenjuk ◽  
M. I. Bereziyk ◽  
M. V. Yurkevych

Aim of our war was to investigate the degradation of diazinon in the model mixture in comparison with the experimental preparation. Methyl alcohol was used as a basis for the manufacture of diazinon drops and the model mixture. The concentration of diazinon was determined by reversed-phase high performance liquid chromatography on the third, fourteenth and twenty-first day after preparation of the model mixture and experimental preparation when stored at room temperature. Despite the large number of publications studying the degradation of diazinon under the influence of various factors, there is a need to study this process in mixtures of a specific composition, in particular in new veterinary drugs that have not yet been studied. This article presents the results of determining the content of diazinon in 10% of the drug and the model mixture based on methyl alcohol. The analyzes were performed on a Knauer liquid chromatograph with a spectrophotometric detector equipped with a Luna® Omega Polar C 18 150 × 4.6 mm column filled with a 5 μm particle sorbent from Phenomenex with a universal C 18 pre-column 4 × 3.0 mm ID. Mobile phase: degassed mixture of acetonitrile: water in the ratio 65:35, flow rate was 1.1 ml/min, temperature of the column was ambient. Diazinone was detected at 245 nm. Injection volume was 0.050 ml and the time of one separation – 20 minutes. The appearance of an unknown peak on the 3 min chromatogram of the solution of the production experimental preparation was revealed. On the twenty-first day of storage of the experimental drug, the content of diazinon decreased to 34 % of the stated amount. On the third and twenty-first day of storage of the model mixture, a decrease in the content of diazinon by 16 % and 79%, respectively, and the appearance of an additional peak were observed. The use of methyl alcohol with other components is likely to be a factor in the breakdown of diazinon in the drug. According to the recipe of the drug, methanol is about half the content of ingredients. This composition does not provide stability of the proposed dosage form of the drug. The next step will be to study the effect of other solvents on maintaining the stated concentration of diazinon.


2007 ◽  
Vol 177 (4S) ◽  
pp. 295-295
Author(s):  
Michael Mullerad ◽  
Haleem J. Issaq ◽  
Alexander Kravtsov ◽  
Timothy Waybright ◽  
Brian Luke ◽  
...  

2013 ◽  
Vol 83 (2) ◽  
pp. 122-128 ◽  
Author(s):  
Cécile Renaud ◽  
Jacques Berger ◽  
Arnaud Laillou ◽  
Sylvie Avallone

Vitamin A deficiency is still one of the major public health problems in least developed countries. Fortification of vegetable oils is a strategy implemented worldwide to prevent this deficiency. For a fortification program to be effective, regular monitoring is necessary to control food quality in the producing units. The reference methods for vitamin A quantification are expensive and time-consuming. A rapid method should be useful for regular assessment of vitamin A in the oil industry. A portable device was compared to high-performance liquid chromatography (HPLC) for three plant oils (rapeseed, groundnut, and soya). The device presented a good linearity from 3 to 30 mg retinol equivalents per kg (mg RE.kg- 1). Its limits of detection and quantification were 3 mg RE.kg- 1 for groundnut and rapeseed oils and 4 mg RE.kg- 1 for soya oil. The intra-assay precision ranged from 1.48 % to 3.98 %, considered satisfactory. Accuracy estimated by the root mean squares error ranged from 3.99 to 5.49 and revealed a lower precision than HPLC (0.4 to 2.25). Although it offers less precision than HPLC, the device estimates quickly the vitamin A content of the tested oils from 3 or 4 to 15 mg RE.kg- 1.


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