scholarly journals Interactions between plant-derived oestrogenic substances and the mycoestrogen zearalenone in a bioassay with MCF-7 cells

2017 ◽  
Vol 20 (3) ◽  
pp. 513-520 ◽  
Author(s):  
Sabine Hessenberger ◽  
K. Botzi ◽  
C. Degrassi ◽  
P. Kovalsky ◽  
C. Schwab ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Oestrogen Receptor ◽  
Proliferative Effect ◽  
Stimulate Cell ◽  
Incubation Experiments ◽  
The Individual ◽  
Stimulate Cell Proliferation ◽  
Mcf 7

Abstract Human and animal diets may contain several non-steroidal oestrogenic compounds which originate either from plants (phytoestrogens) or from fungi that infect plants (mycoestrogens such as zearalenone (ZEN)). Phytoestrogens may compete with ZEN in binding to the oestrogen receptor β and thereby may counteract the oestrogenic activity of ZEN. Using a modified version of the E-screen assay, plant-derived oestrogenic substances were tested for their proliferative or anti-proliferative effect on oestrogen-dependent MCF-7 cells. The samples were additionally tested for their ability to influence the oestrogenic activity of ZEN (1 μM). Among the individual substances tested, 8-prenylnaringenin had the strongest effect, as cell proliferation was increased by 78% at the lowest concentration (0.23 μM), and by 167% at the highest concentration (29.4 μM). Coumestrol (5.83 μM) increased cell proliferation by 39%, and genistein (370 μM) by 61%, respectively. Xanthohumol and enterolactone did not stimulate cell proliferation significantly. In the co-incubation experiments with ZEN, none of the single substances was able to decrease the oestrogenic activity of ZEN. Only for 8-prenylnaringenin (14.7 and 29.4 μM) was a trend towards an increase in the ZEN-induced cell proliferation up to 72% observed. In conclusion, with the exception of 8-prenylnaringenin, no substantial interaction between phytoestrogens and the mycotoxin ZEN could be detected using a bioassays with MCF-7 cells.

Proliferative effect of whey from cows’ milk varying in phyto-oestrogens in human breast and prostate cancer cells

2012 ◽  
Vol 79 (2) ◽  
pp. 143-149 ◽  
Author(s):  
Tina S. Nielsen ◽  
Annika Höjer ◽  
Anne-Maj Gustavsson ◽  
Jens Hansen-Møller ◽  
Stig Purup
Keyword(s):  
Prostate Cancer ◽  
Cell Proliferation ◽  
Cancer Cells ◽  
Dietary Treatment ◽  
Human Breast ◽  
Prostate Cancer Cells ◽  
Proliferative Effect ◽  
Significant Difference ◽  
Breast And Prostate Cancer ◽  
Mcf 7

Intake of dietary phyto-oestrogens has received a great deal of attention owing to their potential influence on hormone-sensitive cancers such as breast and prostate cancer. Cows’ milk contains phyto-oestrogens and the content varies according to the composition of the feed and the type and amount of legumes used. In this study we evaluated the proliferative effect of milk (whey) with different phyto-oestrogen content in human breast (MCF-7) and prostate cancer cells (PC-3). Milk was obtained from cows fed either a birdsfoot trefoil-timothy silage based ration (B1) or two different red clover silage based diets (R1 and R2) resulting in total phyto-oestrogen contents of 403, 1659 and 1434 ng/ml for the B1, R1 and R2 diets, respectively. Whey was produced from the milk and added to cell culture medium in concentrations up to 10% for MCF-7 cells and 5% for PC-3 cells. Cell proliferation was measured fluorometrically after 7 d for MCF-7 cells and 5 d for PC-3 cells. There was no significant difference in the proliferative effect of whey from the different dietary treatments at any of the whey concentrations tested. An anti-proliferative effect (P<0·01) of 5 and 10% whey was seen when tested in the presence of 10 pmoestradiol in the medium. This effect was independent of dietary treatment of cows. Whey induced a significant (P<0·01) proliferative response in PC-3 cells independent of dietary treatment. Purified equol in concentrations similar to equol concentrations in milk decreased PC-3 cell proliferation, and therefore the stimulatory effect of whey in PC-3 cells is believed to be mediated by other bioactives than equol. In conclusion, our results suggest that using whey in these proliferation assays, it was not possible to discriminate between milk with high or low levels of phyto-oestrogens.

Micro-mechanical forces stimulate cell proliferation and vascularization of perfused tissues

2006 ◽  
Vol 203 (3) ◽  
pp. S60
Author(s):  
Giorgio Pietramaggiori ◽  
Jeffrey Rentz ◽  
Saja Scherer ◽  
Kaipainen Arja ◽  
Perry Liu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Mechanical Forces ◽  
Stimulate Cell ◽  
Stimulate Cell Proliferation

scholarly journals Developing oligodendrocytes express functional GABABreceptors that stimulate cell proliferation and migration

2007 ◽  
Vol 100 (3) ◽  
pp. 822-840 ◽  
Author(s):  
Karen Luyt ◽  
Timothy P. Slade ◽  
Jienchi J. Dorward ◽  
Claire F. Durant ◽  
Yue Wu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Proliferation And Migration ◽  
Stimulate Cell ◽  
And Migration ◽  
Stimulate Cell Proliferation ◽  
Proliferation And Migration

Drug with Neuroprotective Properties Noopept Does Not Stimulate Cell Proliferation

2019 ◽  
Vol 166 (4) ◽  
pp. 466-468
Author(s):  
L. F. Zainullina ◽  
T. V. Ivanova ◽  
R. U. Ostrovskaya ◽  
T. A. Gudasheva ◽  
Yu. V. Vakhitova ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stimulate Cell ◽  
Stimulate Cell Proliferation

Synergistic cytotoxic effects of tamoxifen and black cohosh on MCF-7 and MDA-MB-231 human breast cancer cells: an in vitro studyThis article is one of a selection of papers published in this special issue (part 2 of 2) on the Safety and Efficacy of Natural Health Products.

2007 ◽  
Vol 85 (11) ◽  
pp. 1153-1159 ◽  
Author(s):  
Mahéra Al-Akoum ◽  
Sylvie Dodin ◽  
Ali Akoum
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Cell Growth ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Cytotoxic Effect ◽  
Black Cohosh ◽  
Proliferative Effect ◽  
Dose Dependent ◽  
Mcf 7

Breast cancer cell cultures were exposed to different concentrations of black cohosh, estradiol (E2), and tamoxifen to examine the effect on cell proliferation; cytotoxicity was assessed by using sulforhodamine B (SRB) dye solution. E2 (10−10–10−8 mol/L) markedly stimulated the proliferation of MCF-7 cells (p < 0.01). Tamoxifen stimulated MCF-7 cell proliferation at 10−6 mol/L and 10−5 mol/L (p < 0.005) but inhibited in a dose-dependent fashion the proliferative effect of E2 (p < 0.001). Black cohosh alone did not show any stimulatory effect, but exhibited a cytotoxic effect, which was significant at 103 μg/mL (p < 0.001). Adding black cohosh at 100–103 μg/mL to E2 at 10−9 mol/L also resulted in a dose-dependent inhibition of E2 proliferative effect. Interestingly, the combination of black cohosh (100–103 μg/mL) with increasing tamoxifen concentrations further inhibited MCF-7 cell growth. On MDA-MB-231 cells, neither E2 nor tamoxifen displayed any detectable effect. However, black cohosh inhibited MDA-MB-231 cell proliferation at 103 μg/mL (p < 0.05), and this inhibitory effect was enhanced by increasing tamoxifen concentrations. This study reveals a cytotoxic effect of black cohosh on both estrogen-sensitive and estrogen-insensitive breast cancer cells and a synergism with tamoxifen for inhibition of cancerous cell growth.

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