The Oocytes of the Goby Pomatoschistus minutus. III. Determination of Amino Acid Component

1980 ◽  
Vol 35 (11-12) ◽  
pp. 1094-1095
Author(s):  
Rüdiger Riehl
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Acid Content ◽  
Amino Acid Content ◽  
Acid Component ◽  
Pomatoschistus Minutus ◽  
Protein Amino Acids ◽  
Amino Acid Component

Abstract The oocytes of the marine goby Pomatoschistus minutus were analyzed for their amino acid content. Most of the amino acids exist as protein, only a little part is free or peptide-bound. Among the protein-bound amino acids, high levels of glutamic acid, proline, alanine, aspartic acid, valine and leucine were detected. These represent more than 60% of the protein amino acids. Among the free acids, glutamic acid, serine and alanine, are dominant. There are no certain proofs of the occurrence of peptide pools in the oocytes of Pomatoschistus minutus.

scholarly journals Chromatographic Method for Determination of the Amino Acid Content in Dioscorea bulbifera L. Tubers by RP-HPLC

2019 ◽  
Vol 25 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Musadiq Hussain Bhat ◽  
Mufida Fayaz ◽  
Amit Kumar ◽  
Alamgir Ahmad Dar ◽  
Ashok Kumar Jain
Keyword(s):  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Acid Content ◽  
High Performance ◽  
Amino Acid Content ◽  
Column Temperature ◽  
Dioscorea Bulbifera

Background: The present study was carried out for determination of amino acid content in tubers of Dioscorea bulbifera using reverse-phase high-performance liquid chromatography. Methods: The method involved the vapor phase hydrolysis of the sample, automated derivatisation of the amino acids with the aid of AccQ-Fluor reagent kit, separated on a high performance liquid chromatography equipped with photo diode array (HPLC-PDA) at 254 nm having column temperature of 37 ºC. Results: The proportional molar concentration for each amino acid was calculated based on the concentration of standard amino acids and expressed as μg amino acid/mg sample. Methionine, aspartic acid and leucine were major components while as tyrosine was found minor from the plant on dry weight basis. Conclusion: The method is reliable, simple and economical for determining the amino acid content of Dioscorea bulbifera tubers.

scholarly journals Effect of Growing Seasons, Locations and Planting Dates on Total Amino Acid Composition of Two Valencia Peanut Varieties Grown in New Mexico1

1981 ◽  
Vol 8 (2) ◽  
pp. 131-133 ◽  
Author(s):  
David C. H. Hsi ◽  
Clyde T. Young ◽  
Melchor Ortiz
Keyword(s):  
Amino Acids ◽  
New Mexico ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Acid Content ◽  
Amino Acid Content ◽  
Planting Date ◽  
Total Amino Acid

Abstract Two Valencia peanut (Arachis hypogaea L.) cultivars, New Mexico Valencia A and New Mexico Valencia C, were grown at Arch and Los Lunas, N. M. All peanuts were grown under either sprinkler or furrow irrigation. Samples were obtained following harvest, hydrolyzed and analyzed in Raleigh, N. C. for amino acid content. With samples from Arch, no variety by planting date effects were noted for amino acid composition. With samples from Los Lunas, however, significant variety by planting date effects were noted for glutamic acid, glycine, methionine, isoleucine, and leucine. Planting date effects were found only for glycine at the Los Lunas location and phenylalanine at both locations. Significant variety differences were found for methionine, tyrosine, phenylalanine, and lysine at the Arch location and for glycine, methionine, phenylalanine, and lysine at the Los Lunas location. A significant year effect, although small, was noted for about half of the amino acids (aspartic acid, serine, glutamic acid, proline, glycine, tyrosine, phenylalanine, arginine, and the sum of all amino acids) studied at the Arch location. Data at Los Lunas were observed only during the second year. The amino acid content agrees closely with that previously reported for other types of peanuts except for a 100% higher level of cystine found in this study.

scholarly journals Komposisi Kandungan Asam Amino Pada Teripang Emas (Stichoupus horens) di Perairan Pulau Bintan, Kepulauan Riau

2018 ◽  
Vol 6 (2) ◽  
pp. 186-192
Author(s):  
Gianto Gianto ◽  
Made Suhandana ◽  
R. Marwita Sari Putri
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Sea Cucumber ◽  
Acid Value ◽  
Essential Amino Acids ◽  
Sea Cucumbers ◽  
High Protein Content ◽  
Acid Component ◽  
Amino Acid Component

This study aims to analyze the amino acid component contained in the golden sea cucumber so that it can be used as a basis for consideration, management, especially the development of sea cucumbers. Protein in sea cucumbers have complete amino acids, both essential amino acids and non-essential amino acids. The result of the proximate test of golden sea cucumber has high protein content which is 95,14% and fat 0,20% compared to other sea cucumber type while the protein of sea cucumber is very low that is 1.78% and 1.54% ash. The highest content of dried sea cucumber amino acids in golden sea cucumber meat seen in glutamic acid of 6.6049%, 7.1769% glycine, aspartic acid 3.9227% and proline of 3.4189% while the results of wet sea cucumber amino acids have a low amino acid value with glutamic acid 0.2281%, Glycine 0.2308% and Alanin 0.2169%.

scholarly journals Changes in the Pree Amino Acids of the Blood of Blowfly Larvae At Metamorphosis

1956 ◽  
Vol 9 (3) ◽  
pp. 400 ◽  
Author(s):  
RH Hackman
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Free Amino Acid ◽  
Acid Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Content ◽  
Free Amino Acid Content ◽  
Metabolic Significance

Blood from the larval, prepupal, and early pupal stages of Calliphora augttr' (F.) contains the same 18 free amino acids. In addition, hydroxyproline is present in larval and prepupal blood. The quantitative differenccs in the concent,ratiolls of these amino acids arc reported. Larval blood has the highest free amino acid content (6'6 mgjml) followcd by early pupal blood (4'6 mgjml) and prepnpal blood (3'3 mgjml). As the larva matures to the prepupa, the greatest decreases occm in the concentrations in the blood of alanine, glutamic acid, glutamine, glycine, proline, and tyrosine. The metabolic significance of these changes are discussed.

scholarly journals Rapid Determination of Amino Acids of Nitraria tangutorum Bobr. from the Qinghai-Tibet Plateau Using HPLC-FLD-MS/MS and a Highly Selective and Sensitive Pre-Column Derivatization Method

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1665 ◽  
Author(s):  
Wu Zhou ◽  
Yuwei Wang ◽  
Fang Yang ◽  
Qi Dong ◽  
Honglun Wang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Rapid Determination ◽  
Amino Acid Content ◽  
Tibet Plateau ◽  
Relative Standard ◽  
Nitraria Tangutorum ◽  
Qinghai Tibet Plateau

Amino acids are indispensable components of living organisms. The high amino acid content in Nitraria tangutorum Bobr. fruit distinguishes it from other berry plants and is of great significance to its nutritional value. Herein, using 10-ethyl-acridine-3-sulfonyl chloride as a fluorescent pre-column labeling reagent, a method for the efficient and rapid determination of amino acid content in N. tangutorum by pre-column fluorescence derivatization and on-line mass spectrometry was established and further validated. The limits of detection (signal-to-noise ratio = 3) were between 0.13 and 1.13 nmol/L, with a linear coefficient greater than 0.997 and a relative standard deviation between 1.37% and 2.64%. In addition, the method required a short analysis time, separating 19 amino acids within 20 min. Subsequently, the method was used to analyze the amino acid content of Nitraria tangutorum Bobr. from tissues retrieved from seven regions of the Qinghai-Tibet Plateau. Nitraria tangutorum Bobr. was shown to contain a large amount of amino acids, with the total content and main amino acid varying between the different tissues. This research supports the nutritional evaluation, quality control, and development and utilization of Nitraria tangutorum Bobr.

scholarly journals Amino acids in animal feed: significance and determination techniques

2021 ◽  
Vol 854 (1) ◽  
pp. 012082
Author(s):  
M Sefer ◽  
R B Petronijevic ◽  
D Trbovic ◽  
J Ciric ◽  
T Baltic ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Animal Feed ◽  
Accurate Determination ◽  
Amino Acid Content ◽  
Analytical Techniques ◽  
Normal Structure ◽  
And Function

Abstract Amino acids are fundamental for animal nutrition. Their presence is necessary to maintain the normal structure and function of the intestine, and they are key in regulating metabolic pathways for improving health, survival, growth, development, lactation, and reproduction. The animal feed industry invests great resources and efforts to obtain optimal formulations in which the composition of amino acids plays a key role. In support of these aspirations in recent decades, much attention has been paid to the development and improvement of analytical techniques for the reliable, rapid and accurate determination of amino acid content in animal feed. This paper outlines different methodologies for the analysis of amino acid content in animal feed. Various methods, based on different analytical techniques, are presented for determination of amino acids in feed for nutritional and regulatory purposes.

Comparisons of amino acid and protein levels in oil-extracted seeds of Brassica and Sinapis species, with observations on environmental effects

2000 ◽  
Vol 80 (1) ◽  
pp. 169-174 ◽  
Author(s):  
J. M. Bell ◽  
G. Rakow ◽  
R. K. Downey
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Crude Protein ◽  
Amino Acid Content ◽  
Protein Levels ◽  
Protein Amino Acids ◽  
Amino Acid Levels ◽  
Lower Magnitude ◽  
Dispensable Amino Acids

Seeds from 21 selections of Brassica and Sinapis were analyzed for crude protein (CP; N × 6.25) and amino acid content following oil extraction and grinding. In exp. 1 three cultivars of B. napus, B. rapa and B. juncea were grown in four locations in 4 successive years, with duplicate samples taken from each plot. Brassica juncea contained more CP (P < 0.001) than B. napus or B. rapa (44.6 > 40.1 > 38.8%, respectively). Location affected CP level but not the ranking among cultivars. Year-to-year effects on CP levels (P < 0.001) were of lower magnitude than location effects. Treatments (cultivar, location, year) affected CP and amino acid levels differently. Amino acid levels expressed as percent of CP differed among many cultivars (P < 0.01). Threonine, valine, histidine, lysine and arginine were strongly influenced by location (P < 0.001), as were the dispensable amino acids serine, glutamine and tyrosine. In exp. 2, 10 cultivars and strains grown in one location and in three replicates resulted in greater CP levels in two B. juncea cultivars than in B. napus and B. rapa (42.9 and 43.2 vs. 39.7 and 38.8%), respectively, but lower levels of lysine and threonine in the CP (lysine: 5.4 vs. 6.3; threonine: 3.5 and 3.7 vs. 3.8 and 4.0%). Brassica napus and B. rapa ranked among the lowest in percent CP. In exp. 3, comparisons of eight samples from B. napus, B. rapa and Sinapis alba plots revealed a CP range of 38.1 to 45.0% but differences in amino acid content of the CP were relatively small. Key words: Brassica, Sinapis, protein, amino acids

scholarly journals PROFIL PROTEIN DAN ASAM AMINO KEONG IPONG-IPONG (Fasciolaria salmo) PADA PENGOLAHAN YANG BERBEDA

2013 ◽  
Vol 8 (1) ◽  
pp. 77
Author(s):  
Sri Purwaningsih ◽  
Ella Salamah ◽  
Gian P Apriyana
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Amino Acid Content ◽  
Treatment Method ◽  
Essential Amino Acids ◽  
Before And After ◽  
Weight Calculation ◽  
Improve Health

Ipong-Ipong (Fasciolaria salmo) snail is a mollusk from marine gastropod class in Cirebon, West Java which is consumed by public and believed to improve health, but no research on the protein and amino acid content. This study aimed to determine the proximate content, acid insoluble ash, amino acids (before and after treatment), and content of taurine on the best methods. The research was conducted in two parts. The first part covers sampling, identification, preparation, determination of size and weight, calculation of body recovery, processing, and organoleptic tests. The second part consists of processing treatments boiling (100ºC for 15 minutes), steaming (100ºC for 20 minutes), and boiling with the addition of 3% salt for 15 minutes. The results showed that the treatment gives a significant effect in fat, protein, water, ash and acid insoluble ash content (α=0.05). The highest content of essential amino acids in Ipong-ipong snail was arginine and non-essential amino acid was glutamic. The treatment method causes decreased in amino acid content. Steaming resulted in a decrease of taurine content of 164.17 mg/100 g to 149.62 mg/100 g.<br /> <br /><br />

scholarly journals AMINO ACID STATUS OF BOVINE MILK UNDER SEASONAL VARIATION AROUND SINGRAULI DISTRICT

2021 ◽  
pp. 1-4
Author(s):  
RAJESH K. PATEL ◽  
P. K. SINGH ◽  
RAJESH PANDEY ◽  
BABBULAL PATEL ◽  
SANDEEP MISHRA
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Acid Content ◽  
Bovine Milk ◽  
Amino Acid Content ◽  
Buffalo Milk ◽  
Milk Samples ◽  
Acid Status

Objective: The present work deals with the concerned study to determine the influence of variable season on amino acid evaluation from 90 bovine milk collected (March 2018 to February 2019) samples belongs to four tehsils of Singrauli district. Methods: An aliquot of hydrolysate of collected bovine milk samples were injected into the column (Shim-pack ISC-07/S1504 Na) of the higher performance liquid chromatography-based amino acid analyzer. Results: Most abundant amino acids were glutamic acid followed by proline and leucine. Average amino acid content comparison, results, in the rainy season, maximum amino acid content was in Deosar and minimum in Singrauli, In winter amino acid is higher in Waidhan and minimum in Deosar and maximum amino acid content was recorded intended for Waidhan and minimum for Deosar in summer. Concentration of amino acid in cow milk was found to be in order of glycine<histidine<alanine<methionine<isoleucine<phenylalanine<threonine<tyrosine<serine<lysine<valine and aspartic acid<arginine<leucine<proline<glutamic acid. Amino acid status in buffalo milk sample was found in order Glycine<histidine<methionine<alanine<phenylalanine<isoleucine<threonine<arginine<tyrosine<serine and lysine<aspartic acid<valine<leucine<proline<glutamic acid. All the amino acids are evaluated as per the mean (n = 6) mean±SD. Conclusion: It is concluded that cow and buffalo milk samples contained all the amino acids but differed in the contents of certain amino acids. Such variation is purely biological and genetic and is intended for the nourishment of the young. The current investigation would be valuable for the dairy processing industries to formulate nutritionally improved milk-based functional products for a vulnerable segment of the population.

scholarly journals Absorption of Chemically Unmodified Lysine from Proteins in Foods That Have Sustained Damage During Processing or Storage

2005 ◽  
Vol 88 (3) ◽  
pp. 949-954 ◽  
Author(s):  
Paul J Moughan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Hydrolysis ◽  
Acid Content ◽  
Amino Acid Analysis ◽  
Amino Acid Content ◽  
Food Proteins ◽  
New Compounds

Abstract When a food is processed or stored, amino acids can react with a number of chemical entities to produce new compounds that are often nutritionally unavailable to the consumer. During acid hydrolysis used in amino acid analysis, some of these compounds revert back to the parent amino acid, leading to errors in estimates of both the amino acid content of foods and amino acid digestibility. This is a particular concern for the amino acid lysine in damaged food proteins. Chemical assays have thus been developed to allow determination of unaltered or reactive lysine. However, there is evidence that, in damaged food proteins, not all of the reactive lysine is released during digestion and absorbed. The development and application of an assay for absorbed (ileal digestible) reactive lysine is discussed.

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