scholarly journals Optimization of the extraction and precipitation process of a leaf protein concentrate from Moringa oleifera Lam.

2022 ◽  
Vol 75 (1) ◽  
Author(s):  
Byron Patricio Pérez Simba ◽  
Javier Alberto Garrido Espinosa ◽  
Andrea Belén Endara Vargas ◽  
Andrea Carolina Landázuri Flores ◽  
Lucía de los Ángeles Ramírez Cárdenas
Keyword(s):  
Moringa Oleifera ◽  
Protein Extraction ◽  
Extraction Process ◽  
Raw Material ◽  
Leaf Protein ◽  
Precipitation Process ◽  
Randomized Design ◽  
Leaf Protein Concentrate ◽  
Influence Of Ph ◽  
Moringa Oleifera Lam

This study aimed to determine the best extraction and precipitation conditions of Moringa oleifera Lam. leaf protein. The influence of pH (10, 11, 12) and the concentration of NaCl (0, 0.25, 0.5) for the protein extraction process were studied through a Completely Randomized Design (CRD) with factorial arrange 32. The combination of pH 11 and 12 with 0 M NaCl had the best yield (P<0.05). The treatment of pH 11 without NaCl followed a precipitation stage for its purification, and the effect of different levels of pH (4, 4.5, 5) and temperature (40, 60, 80 °C) were evaluated using a CRD with factorial arrange 22 and 6 central points. The temperature did not affect the yield of the process in a significant way and the amount of precipitate was maximized at pH 4 and 4.5. From 100 g of the dry leaf, 7.26±0.19 g of protein was isolated with a recovery of 26.93±0.22 g 100 g-1 from the total protein. Due to their astringency and bitterness, consuming large amounts of Moringa oleifera Lam leaves is not a solution; therefore, obtaining a leaf proteinconcentrate could be useful for diverse applications in nutritional supplements, and as raw material for functional products development.

scholarly journals PENGARUH VARIASI WAKTU PERENDAMAN SAMPEL DAN JUMLAH PELARUT HOMOGENASI TERHADAP PERSENTASE EKTRAK PROTEIN DAUN KELOR (Moringa oleifera)

2020 ◽  
Vol 7 (1) ◽  
pp. 51-56
Author(s):  
Meirita Sari ◽  
Methatias Ayu Moulina
Keyword(s):  
Immersion Time ◽  
Moringa Oleifera ◽  
Protein Extraction ◽  
Health Sector ◽  
Extraction Process ◽  
Food Plants ◽  
Leaf Protein ◽  
Protein Extract ◽  
Salting Out ◽  
Science And Education

This research is one form of utilization of food plants, namely Moringa (Moringa oleifera). M. oleifera can grow well in Bengkulu Province. The leaves of M. oleifera contain protein compounds that behave like lectins. This compound can be used as an antimicrobial material. The stages in this study include the extraction of M. oleifera leaf protein using salting in and salting out methods. This study aims to determine the effect of the immersion time and the amount of solvent in the homogenation stage extraction process on the percentage of protein extract. The results showed that the immersion time had a significant effect compared to the amount of solvent in the homogenation stage of Moringa oleifera leaf protein extraction. The longer the time needed, the greater the percentage of M. oleifera leaf protein extract. This research is useful for researchers, the public, the health sector, and the development of science and education.     Keywords: Moringa oleifera, Extraction, Protein

scholarly journals Application of Moringa in the Removal of Salts from the Desalinator Reject

2019 ◽  
pp. 1-13
Author(s):  
A. J. Gomes Filho ◽  
S. C. de Paiva ◽  
G. M. C. Takaki ◽  
A. S. Messias
Keyword(s):  
Contact Time ◽  
Moringa Oleifera ◽  
Chemical Elements ◽  
Saline Water ◽  
Physical Chemical ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Whole Seed ◽  
Semi Arid ◽  
Moringa Oleifera Lam

This study aims to analyze the reject produced by the desalinators implanted in the semi-arid Pernambuco in contact with the seeds of Moringa oleifera Lam. For this, Moringa seeds were collected and prepared with the following treatments: reject (control), reject with whole seed with and without husks, reject with crushed seed with and without husks, seed residue with and without husks, in five replicas. Each replica was constituted with doses equivalent to 2.0 g of Moringa seeds for 200 mL of reject, with contact time corresponding to 30, 60, 120 and 180 minutes, in a completely randomized design, under laboratory conditions, total of 140 experimental units. The physical-chemical and statistical analyses were performed through analysis of variance (ANOVA), and the F test, at confidence interval of 95%. It has been found through laboratory tests that seeds with crushed or ground Moringa husks are equally effective at adsorbing sodium from 1,868,0 mg/L to 24,6 mg/L (98,7 %), calcium from 1,005.0 mg/L to 894.6 mg/L (11%), magnesium from 741.0 mg/L to 728.3 mg/L (1.7%) and chloride from 6,997.5 mg/L to 6,782.4 mg/L (1.8%). Therefore, Moringa seed can be considered promising in the adsorption of chemical elements of saline water desalinizers.

scholarly journals ANATOMICAL STUDY OF MORINGA LEAF (MORINGA OLEIFERA LAM.) UNDER IRON FERTILIZATION

2020 ◽  
Vol 21 (supplement 1) ◽  
Author(s):  
Jawad Kadhim Obaid Al-Hujayri ◽  
Abdoun H. Alwan ◽  
Neepal Imtair Al-garaawi
Keyword(s):  
Moringa Oleifera ◽  
Foliar Application ◽  
Anatomical Study ◽  
Iron Mineral ◽  
Randomized Design ◽  
The Mean ◽  
Completely Randomized Design ◽  
Normal Epidermis ◽  
Different Levels ◽  
Moringa Oleifera Lam

The effect of different levels of nano- and mineral-Iron and their method of application on anatomical properties of the upper and lower epidermis of Moringa (Moringa oleifera Lam.) leaves was studied. Seeds of Moringa were sown in 15 kgs soil capacity pots during the growing season of 2018/2019 arranged as a factorial experiment within Completely Randomized Design, with three replicates. The factorswere a method of fertilization(soil or foliar application),three levels i.e. 0,180 and 360mg.l-1 of each of mineral and nano-Iron. The total number of experimental units was 2×3×3×3 for method, nano-Iron, mineral-Iron, and replicates respectively.The application method caused an increase in the mean dimensions of normal epidermis cells, stomatoes, and hairs.These traits were increased as Iron levels increased. Where 360 mg.l-1 of both types of Iron gave the highest dimensions. Soil application gave higher values of epidermis dimensions than that obtained from the foliar application. The number of stomatoesincreased with increasing the level of Iron. The number of normal epidermis cells was conversely proportioned with their volume.

scholarly journals Pengaruh penggunaan Beberapa level Tepung Daun Kelor (Moringa oleifera Lam) dalam Ransum Basal Terhadap Plasma Metabolit Ayam Broiler

2021 ◽  
Vol 11 (1) ◽  
pp. 82
Author(s):  
Edi Erwan ◽  
Santika Yulia Wulandari ◽  
Evi Irawati
Keyword(s):  
Vitamin C ◽  
Total Cholesterol ◽  
Total Protein ◽  
Broiler Chickens ◽  
Moringa Oleifera ◽  
Plasma Metabolites ◽  
Nutrient Contents ◽  
Randomized Design ◽  
Basal Ration ◽  
Moringa Oleifera Lam

Abstract  Moringa leaves (Moringa oleifera Lam) is one of the potential local feed ingredients of broiler rations due to its nutrient contents such as carbohydrate (57,01%), crude protein (30,30%), crude fat (2,74%) as well as a source of vitamin C, carotenoids and flavonoids. This study aimed to determine the effect of using different levels of Moringa oleifera Lam leaf meal (MOLM) in basal ration on plasma metabolites, including total cholesterol (TCHO), triglycerides (TG), glucose (GLU), and total protein (TP). The research design of this study was a completely randomized design (CRD) with four treatments and five replications. The treatments were 4 levels of MOLM (0%, 5%, 10% and 15%), in basal ration. The parameters measured were TCHO, TG, Glu, and TP of in blood plasma. The results showed that inclusion of MOLM up to 15% of in basal ration very significantly (P<0.01) lowering levels TG and decreasing Glu. However, the inclusion of MOLM had no effect (P>0.05) on TCHO and TP. It is concluded that moringa leaf flour addition to the basal ration up to 15% could be used to decrease plasma metabolites especially TG and GLU in broiler chickens.  Key words:Broilers; Moringa oleifera meal; total cholesterol, triglycerides, glucose  and total protein   Abstrak  Daun kelor (Moringa oleifera Lam) merupakan salah satu bahan pakan lokal yang berpotensi dapat dimanfaatkan sebagai bahan penyusun ransum ayam ras pedaging karena mengandung nutrisi yang tinggi diantaranya karbohidrat (57,01%), protein kasar (30,30%), lemak kasar (2,74%) dan sebagai sumber vitamin C, karotenoid serta flavonoid. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan beberapa level tepung daun kelor  dalam ransum basal terhadap plasma metabolit ayam ras pedaging yang meliputi total kolesterol (TCHO), trigliserida (TG), glukosa (Glu) dan total protein (TP). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan empat perlakuan dan lima ulangan. Perlakuan yang diberikan terdiri dari 4 level tepung daun kelor di dalam ransum basal yakni 0%, 5%, 10% dan 15%. Parameter yang diukur meliputi TCHO, TG, Glu dan TP pada plasma darah. Hasil penelitian menunjukkan bahwa pemanfaatan tepung daun kelor hingga 15% dalam ransum berpengaruh nyata (P<0,05) menurunkan kadar TG dan berpengaruh sangat nyata (P<0,01) menurunkan Glu. Akan tetapi, pemanfaatan tepung daun kelor hingga 15% tidak berpengaruh nyata (P>0,05) terhadap TCHO dan TP. Kesimpulan penelitian ini adalah penggunaan tepung daun kelor dalam ransum basal hingga 15% dapat dimanfaatkan untuk menurunkan plasma metabolit khususnya TG dan Glu pada  plasma darah ayam broiler.   Kata kunci : Ayam ras pedaging; tepung daun kelor; plasma metabolit

scholarly journals Profiling of Collagens from Swim Bladder of Catfish (Pangasius sp.) by Acid Extraction

2019 ◽  
Vol 22 (2) ◽  
pp. 327-339
Author(s):  
Gressty Sari Br Sitepu ◽  
Joko Santoso ◽  
Wini Trilaksani
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Triple Helix ◽  
Extraction Process ◽  
Raw Material ◽  
Acid Extraction ◽  
Swim Bladder ◽  
Acid Pretreatment ◽  
Randomized Design ◽  
Completely Randomized Design

Swim bladders of catfish contain high protein, therefore it is can be used as a raw material for collagen. The study aims to determine the chemical characteristics of swim bladders, the pretreatment of non-collagen, extraction of collagen dissolves acid and to evaluate the characteristics of collagen. The method of this study is KOH pretreatment with a concentration of 0,05; 0,1; and 0,15 for 12 hours. The extraction process is done by soaking the sample in a solution of acetic acid with a concentration of 0,25; 0,5 and 0,75 M (ratio 1:10; b/v) and extraction time for 24; 48; and 72 hours at 4oC. The experimental design used for alkaline and acetic acid pretreatment were factorial completely randomized design. The result showed that the protein content of swim bladder was 85,26% (db), the profile of amino acids were dominated by three amino acids namely glycine (56,85 mg/g), prolyne (31,03 mg/g), and alanyne (23,85 mg/g). Using 0,05 M KOH for 8 h was selected as the best pretreatment method for collagen extraction. Extraction method using 0,50 M acetic for 48 h resulted he best collagen which revealed the existence of a triple helix structure and had Tg 84oC.

scholarly journals Pengaruh Panjang dan Diameter Stek Batang Terhadap Pertumbuhan Bibit Kelor (Moringa oleifera Lam.)

2018 ◽  
Vol 4 (2) ◽  
pp. 120
Author(s):  
Wahyu Astiko ◽  
Ahsani Taqwim ◽  
Bambang Budi Santoso
Keyword(s):  
Moringa Oleifera ◽  
Stem Diameter ◽  
Stem Cutting ◽  
Stem Length ◽  
Stem Cuttings ◽  
Randomized Design ◽  
Cutting Length ◽  
Nursery Field ◽  
Moringa Oleifera Lam

The purpose of the study is to discover the best length and the diameter of Moringa oleifera Lam. stem cuttings to produce high quality of seedlings. The study was conducted during March-May 2017 located in the nursery field at Dasan Agung, Mataram, 16 m asl. This study was experimental research with Complete Randomized Design with three three replications, and each contains 5 serial experiment units. The treatment for stem cutting length (25 cm, 50 cm, and 75 cm) was then combined with different stem cutting diameters (3-4 cm, 4.1-5 cm, and 5.1-6 cm). The result shows that combination of 75 cm stem length with stem  diameter of 5.1-6 cm resulted to have the best seedling growth.The longer the cutting (up to 75 cm), the better the growth of the seedling. The thickest stem diameter (5.1-6 cm) resulted better seedling

scholarly journals SYNTHESIS AND CHARACTERIZATION OF NICKEL HYDROXIDE FROM EXTRACTION SOLUTION OF SPENT CATALYST

Metalurgi ◽  
2020 ◽  
Vol 35 (3) ◽  
pp. 111
Author(s):  
Kevin Cleary Wanta ◽  
Felisha Hapsari Tanujaya ◽  
Federick Dwi Putra ◽  
Ratna Frida Susanti ◽  
Gelar Panji Gemilar ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Nickel Hydroxide ◽  
Precursor Solution ◽  
Extraction Process ◽  
Rechargeable Batteries ◽  
Raw Material ◽  
Precipitation Method ◽  
Precipitation Process ◽  
Spent Catalyst ◽  
Almost All

Nickel is an essential metal element and is applied in various sectors. One of the useful nickel–based derivatives products is nickel hydroxide [Ni(OH)2]. This compound is widely applied as raw material for electrodes of rechargeable batteries, capacitors, electrolyzers, and catalysts. This study focuses on the synthesis of Ni(OH)2 using the hydroxide precipitation method. A solution from the extraction process of spent catalysts was used as a precursor solution. After the precursor solution was obtained, the precipitation process was carried out at pH 10, where the operating temperature was varied at 30–60oC. NaOH, KOH, and MgO solutions were used as precipitating agents. The experimental results show that the Ni(OH)2 compounds were produced optimally at low temperatures, 30oC. It could be indicated from the lowest concentration of Ni2+ ions in the liquid phase that reached that temperature. The three precipitation agents also gave good results in the precipitation of Ni2+ ions, where almost all of the Ni2+ ions were precipitated from the liquid phase. The precipitated products were characterized using SEM, XRD, and XRF. The analysis results showed that the product was agglomerated and formless. The purity of the precipitates formed were 24.1 and 29% for the precipitating agents MgO and NaOH, respectively.

scholarly journals Pengaruh Berbagai Macam Media terhadap Pertumbuhan Bibit Kelor (Moringa oleifera Lam.) Asal Stek Batang

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Sawaludin Sawaludin ◽  
Aluh Nikmatullah ◽  
Bambang Budi Santoso
Keyword(s):  
Experimental Design ◽  
Experimental Method ◽  
Sea Level ◽  
Moringa Oleifera ◽  
Stem Cutting ◽  
Soil Charcoal ◽  
Randomized Design ◽  
The Media ◽  
Completely Randomized Design ◽  
Moringa Oleifera Lam

This study aimedseedling derived from stem cutting to determine the effect of various media on the growth of drum stick (Moringa oleifera Lam.). Experiment was conducted from March to May 2017 in the nursery located in Dasan Agung, Mataram, with altitude of 16 meters above sea level. The method was experimental method with 6 media treatments: soil, soil+sawdust, soil+cocopeat, soil+corncob, soil+bamboo leaf, and soil+charcoal rice husk. The experimental design was Completely Randomized Design with 5 replications and 5 unit series. The result shows that the media significantly effect on the growth ofsedling stem cutting. The best media forseedling derived from stem cutting were mixture media of soil+ricecharcoal andsoil+cocopeat

scholarly journals Pertumbuhan Bibit Tanaman Kelor (Moringa oleifera Lam.) Pada Beberapa Macam Media Organik dengan Teknik Pembibitan Tidak Langsung

2018 ◽  
Vol 4 (2) ◽  
pp. 132
Author(s):  
Rasmani Rasmani ◽  
I Gusti Made Arya Parwata ◽  
Bambang Budi Santoso
Keyword(s):  
Leaf Litter ◽  
Experimental Research ◽  
Seedling Growth ◽  
Moringa Oleifera ◽  
Organic Media ◽  
Randomized Design ◽  
Soil Media ◽  
Completely Randomized Design ◽  
Nursery Field ◽  
Moringa Oleifera Lam

The experimental research is aimed to find out the kind of nursery organic media for better growth of drumstick seedling. It was implemented in January-April 2017 in the nursery field in Dasan Agung, Mataram. The treatments studied were kinds of nursery media including soil media, mixture of soil-sawdust, mixture of soil-cocopeat, mixture of soil-crushed corncobs, mixture of soil-bamboo leaf litter. The experiment design was arranged in a Completely Randomized Design with 5 replications and each content of 5 serial experiment units. The result shows that the kind of seedling media significantly affected the growth to Moringa seedling growth. The soil-cocopeat mixture medium, soil-crushed corn cobs, and soil-bamboo leaf litter were the best medium and more suitable for drumstick seedling compared to soil media and soil-sawdust mixture media

Sign in / Sign up

Export Citation Format

Share Document