scholarly journals PERFORMA PEMIJAHAN IKAN BELIDA (Notopterus chitala) MELALUI INDUKSI HORMON HCG DAN LHRH

2018 ◽  
Vol 13 (2) ◽  
pp. 115
Author(s):  
Lies Setijaningsih ◽  
Jojo Subagja ◽  
Deni Radona ◽  
Brata Pantjara ◽  
Anang Hari Kristanto ◽  
...  
Keyword(s):  
Body Weight ◽  
Survival Rate ◽  
Fertilization Rate ◽  
Hatching Rate ◽  
Processed Food ◽  
Induction Dose ◽  
Breeding Technology ◽  
Technology Applications ◽  
Egg Diameter ◽  
Lhrh Analog

Permintaan ikan belida cukup tinggi untuk memenuhi kebutuhan konsumsi dan bahan baku olahan makanan yang masih mengandalkan tangkapan dari alam. Tingginya eksploitasi mengakibatkan populasi ikan dari tahun ke tahun semakin berkurang dan terancam punah. Teknologi pembenihan ikan belida dengan penggunaan hormon diduga dapat meningkatkan produksi larva untuk keperluan budidaya. Penelitian dilakukan untuk mengevaluasi performasi pemijahan ikan belida secara alami dan buatan melalui induksi hormon HCG dan LHRH analog. Pemijahan alami dan buatan dilakukan menggunakan induk betina sebanyak enam ekor dan jantan tiga ekor (rasio 2:1). Induk yang digunakan berukuran panjang 46 ± 5 cm dan bobot 2.209 ± 623 g. Pemijahan buatan dilakukan dengan induksi hormon HCG dosis 500 IU/kg dan LHRH analog dosis 0,5 mL/kg. Penyuntikan HCG dan LHRH diberikan dengan interval 24 jam. Hasil penelitian menunjukkan pada pemijahan buatan (induksi hormon) diperoleh induk yang memijah sebanyak dua ekor dengan nilai diameter telur (3,1 ± 0,3 mm); fekunditas (282-907 butir); derajat pembuahan (21%-40%); derajat penetasan (56%-75%); dan sintasan (30%-50%); sedangkan pada pemijahan alami diperoleh satu ekor induk yang memijah dengan nilai diameter telur (3,5 ± 0,3 mm); fekunditas (1.616 butir); dan derajat pembuahan (86,7%); selama tiga bulan pengamatan secara intensif dengan frekuensi pemijahan enam kali. Aplikasi teknologi pemijahan dengan menggunakan induksi hormon dapat menghasilkan produk larva pada ikan belida.The demand for fish consumption of Clown Knifefish is considerably high. However, the fish supply for consumption and processed food still relies on wild-caught. Such exploitation has decreased the population of Clown Knifefish to a point that it can become an endangered species. Efforts on the breeding technology of Clown Knifefish through natural and artificial propagation using of hormones have shown promising results. The current study was conducted to evaluate the performance of Clown Knifefish in natural and artificial spawning conditions using the induction of HCG hormone and LHRH analogues. The natural and artificial spawning treatments were conducted using six fish females and three fish males (a ratio of 2:1). The average Broodstocks’ size was 46 ± 5 cm in length and 2,209 ± 623 g in weight. The artificial spawning was conducted with HCG hormone induction (dose of 500 IU/kg body weight) and LHRH analogue (dose: 0.5 mL/kg). HCG and LHRH injections were given within 24 hours interval. The results showed that the artificial spawning (hormone induction) had successfully caused two broodstock to spawn producing eggs with the diameter of 3.1 ± 0.3 mm; fecundity of 282-907 eggs; fertilization rate of 21%-40%; hatching rate of 56%-75%; and survival rate of 30%-50%. In the natural spawning, one broodstock had spawned with the egg diameter of 3.5 ± 0.3 mm; fecundity of 1,616 eggs; and fertilization rate of 86.7%. This study concludes that spawning technology applications using hormonal induction can increase the production larvae of Clown Knifefish broodstock.

scholarly journals PROFIL HORMON ESTRADIOL 17β DAN INDUKSI HORMON OVAPRIM DALAM PEMIJAHAN BUATAN IKAN SELAIS (Ompok hypophthalmus Blkr)

2018 ◽  
Vol 46 (2) ◽  
pp. 11
Author(s):  
Nasril Ismail ◽  
Usman M Tang ◽  
Henni Syawal
Keyword(s):  
Body Weight ◽  
Survival Rate ◽  
Fertilization Rate ◽  
The Body ◽  
Hatching Rate ◽  
Randomized Design ◽  
Before And After ◽  
Egg Diameter ◽  
Estradiol 17Β

The purpose of this study was analyze the amount of estradiol and tostesterone hormone in the body of the selais (O. hypophthalmus Blkr) fish the mature gonads and the number of ovaprim hormone doses used based on the amount of hormone in the fish body on ovulatory success and spawning of fish Selais. The study used a complete randomized design consisting of four treatments and three replicates consisting of controls, 0.05 mL / 100 grams of body weight, 0.07, mL / 100 gram body weight, 0.09 mL / 100 gram body weight and 0,11 mL / 100 gram body weight. The results showed that the concentrations of estradiol-17β hormone in blood ranged from 199.13 pg / mL to 236.28 pg / mL before and after ovulation of each treatment ranging from 273.97 pg / mL to 873.65 pg / mL and after ovulation ranged from 0.71 pg / mL to 1.78 pg / mL Whereas for other parameters obtained the results of the latency 11.27 ± 0.252 to 13.40 ± 1,95 hours, fecundity 1919 ± 0.546 to 3243 ± 0.420 grains, egg diameter after ovulation 1.32 ± 0.29 to 1.32 ± 0.29 mm, fertilization rate of 28.99±157 to 36.54±148 %, hatching rate of 40.79± 071 to 36.54±148 % and the survival rate 3 day 75.82 ± 047 to 84.53 ± 0.36 %.

scholarly journals REPRODUKSI IKAN BAUNG (Hemibagrus nemurus) DENGAN PERLAKUAN DOSIS HORMON GnRH-a BERBEDA

2018 ◽  
Vol 13 (3) ◽  
pp. 213
Author(s):  
Jojo Subagja ◽  
Vitas Atmadi Prakoso
Keyword(s):  
Body Weight ◽  
Survival Rate ◽  
Larval Survival ◽  
Fertilization Rate ◽  
Fertility Rate ◽  
Time Interval ◽  
Hatching Rate ◽  
Good Prospect ◽  
Hormonal Manipulation ◽  
Different Doses

Ikan baung (Hemibagrus nemurus) memiliki prospek bagus untuk dikembangkan sebagai komoditas budidaya di Indonesia karena citarasa daging yang enak banyak diminati konsumen. Dewasa ini, peningkatan produksi benih dapat dilakukan melalui pemijahan buatan dengan manipulasi hormonal. Penelitian yang dilakukan bertujuan untuk mengevaluasi produksi induk ikan baung melalui pemijahan buatan dengan dosis penyuntikan GnRH-a berbeda. Penelitian dilakukan di Instalasi Riset Plasma Nutfah Perikanan Air Tawar, Cijeruk, Bogor pada bulan November 2017. Induk betina ikan baung yang digunakan berukuran 465,0 ± 71,8 g; dan induk jantan 426,3 ± 46,8 g. Induksi dilakukan dengan penyuntikan hormon GnRH-a dengan tiga dosis yang berbeda pada induk betina (0,3; 0,5; dan 0,7 mL/kg bobot badan), dan ikan jantan dengan dosis 0,4 mL/kg bobot badan. Masing-masing perlakuan terdiri atas tiga ekor induk betina sebagai ulangan. Hormon disuntikkan secara intramuskular, diberikan dua kali penyuntikan 35% dari dosis total pada penyuntikan pertama, dan 65% diberikan pada penyuntikan kedua, dengan interval waktu penyuntikan delapan jam. Parameter yang diamati yaitu jumlah telur ovulasi, derajat pembuahan, derajat penetasan, dan sintasan larva selama tujuh hari. Hasil penelitian menunjukkan bahwa dosis penyuntikan hormon GnRH-a 0,5 mL/kg pada induk ikan baung saat proses pemijahan buatan menghasilkan derajat penetasan yang lebih tinggi dibandingkan dosis 0,3 dan 0,7 mL/kg; serta sintasan larva yang lebih baik dibandingkan dosis 0,7 mL/kg. Sedangkan nilai jumlah telur yang berhasil ovulasi dan derajat pembuahan yang relatif lebih baik ditemukan pada perlakuan dosis 0,7 mL/kg dibandingkan dengan dosis 0,3 dan 0,5 mL/kg.Asian redtail catfish (Hemibagrus nemurus) has a good prospect to be developed as aquaculture commodity in Indonesia. It was proved by the demand of consumers regarding to its delicious flesh taste. Recently, efforts to increase seedling production could be implemented through artificial spawning with hormonal manipulation. This study was conducted to evaluate the production of broodstocks through artificial spawning with different doses of GnRH-a. Study was conducted at Research Institute for Freshwater Fisheries Germplasm, Cijeruk, Bogor in November, 2017. The broodstock used in this study was 465.0 ± 71.8 g for females, and 426.3 ± 46.8 g for males. Induction was performed by injection of GnRH-a hormone with three different doses on female broodstocks (0.3, 0.5 and 0.7 mL/kg of body weight), and male broodstocks with a dose of 0.4 mL/kg of body weight. Each treatment consisted of three females as replications. The hormone was injected intramuscularly, given twice injection: 35% of the total dose is given at the first injection, and the remaining 65% was given at the second injection, with an injection time interval of eight hours. The results showed the observed parameters of number of the ovulated eggs, fertilization rate, hatching rate, and larval survival for seven days, treatment dosage of 0.5 mL/kg showed better results (P<0.05) than the dosage of 0.3 mL/kg and 0.7 mL/kg. Parameters observed were number of ovulated eggs, fertility rate, hatching rate, and survival rate for seven days. The results showed that the dosage of 0.5 mL/kg GnRH-a injection to the broodstock during artificial spawning resulted in higher hatching rate compared to 0.3 and 0.7 mL/kg and better larval survival rate than the dosage of 0.7 mL/kg. Meanwhile, the number of ovulated eggs and fertility rate was relatively better in the treatment of 0.7 mL/kg compared with the dosage of 0.3 and 0.5 mL/kg.

scholarly journals Effect of pituitary gland doses on artificial propagation of Guchibaim, Mastacembelus pancalus (Hamilton)

2012 ◽  
Vol 9 (2) ◽  
pp. 305-310
Author(s):  
MM Rahman ◽  
MI Miah ◽  
KR Hasan ◽  
SM Farid
Keyword(s):  
Body Weight ◽  
Single Dose ◽  
Survival Rate ◽  
Pituitary Gland ◽  
Fertilization Rate ◽  
Ovulation Rate ◽  
Optimum Dose ◽  
Hatching Rate ◽  
Double Dose ◽  
Artificial Propagation

Artificial propagation of Mastacembelus pancalus was conducted in order to determine the optimum dose of pituitary  gland (PG) hormone at the hatchery of the Field Laboratory Complex, Faculty of Fisheries, Bangladesh Agricultural  University, Mymensingh. The present study consisted of two trials and each trial had four treatments (T1, T2, T3 and  T4, respectively) with three replications of each. In the experiment single dose and double dose had been used for T1,  T2, T3 and T4, respectively to evaluate the efficiency on ovulation rate, fertilization rate, and hatching rate of M.  pancalus eggs. The hatchlings were reared in aquarium up to 30 days and survival rate was determined. Treatment-3  (T3) of double dose (170mg PG kg-1 and 60mg PG kg-1 body weight of fish for female and male respectively) showed  better results in terms of ovulation rate (90.03±2.56%), fertilization rate (90±0.81%), hatching rate (80±1.84%), and  survival rate (40.00±3.19%) was recorded than other treatments of single and double dose. The water temperature  was recorded 27 to 31°C. In the present study, treatment-3 (T3 in double dose) was the best findings in terms of  ovulation rate, fertilization rate, and hatching rate which can be used in artificial propagation of M. pancalus for the  development of hatchery production. May and June are the suitable months for artificial propagation of the said  species.   DOI: http://dx.doi.org/10.3329/jbau.v9i2.11045   J. Bangladesh Agril. Univ. 9(2): 305–310, 2011

scholarly journals Induced breeding of the riverine catfish Rita rita

1970 ◽  
Vol 6 (2) ◽  
pp. 361-366
Author(s):  
MFA Molla ◽  
MR Amin ◽  
MN Sarowar ◽  
M Muhammadullah
Keyword(s):  
Body Weight ◽  
Pituitary Gland ◽  
Fertilization Rate ◽  
Hatching Rate ◽  
Induction Of Ovulation ◽  
Successful Induction ◽  
Hatching Rates

The paper reports the first incidence of successful induction of breeding in the riverine catfish Rita rita using carp pituitary gland (PG) extract. A breeding trial using four PG doses viz. 80, 100, 120 and 140mg/kg body weight of fish was conducted to optimize the dose of pituitary gland (PG) extract in terms of induction of ovulation in female. The male received a dose of 40mg PG/kg body weight in all cases and was sacrificed for collection of milt. The best performance was shown by the fish treated with 100mg PG/kg body weight in respect of inducing ovulation in females and fertilization and hatching rates of eggs. Keywords: Induced breeding; Rita rita; Ovulation; Fertilization rate; Hatching rate DOI: 10.3329/jbau.v6i2.4835 J. Bangladesh Agril. Univ. 6(2): 361-366, 2008

scholarly journals Reproductive performance of asian catfish (Hemibagrus wyckii Bagridae), a candidate species for aquaculture

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 683 ◽  
Author(s):  
Netti Aryani ◽  
Indra Suharman ◽  
Hafrijal Syandri
Keyword(s):  
Reproductive Performance ◽  
Fertilization Rate ◽  
Culture Conditions ◽  
Hatching Rate ◽  
Male Fish ◽  
Egg Weight ◽  
Study Results ◽  
Candidate Species ◽  
Asian Catfish ◽  
Egg Diameter

Background: Hemibagrus wyckii Bagridae is one of the most important economic fish species that lives in the rivers and reservoir in Riau Province, Indonesia. The present study aimed to determine the reproductive performance of H.wyckii under culture conditions. Methods: A total of 10 female and 10 male fish were selected, and weight, length, characteristics of egg and sperm, and hatchery performance were measured. Eggs were fertilized using the dry method. Egg weight and egg diameters were measured for 50 eggs per female. Egg size (50 eggs for each fish) was measured using an Olympus microscope (CX40). Then, saline solution was added over the eggs, followed by the addition of pooled sperm from 10 males. Results: Average relative fecundity, egg weight and egg diameter were 2060±512 eggs/kg fish, 29.86±1.21 mg and 2.67±0.26 mm, respectively. The fertilization rate and hatching rate were 60.91±4.68% and 42.91±2.92% respectively. Sperm characteristics such as volume per fish (mL), pH, concentration (per mL), motility (%) and duration of motility (second) were 0.82±0.20, 7.15±0.12, 3.68±0.15, 72.77±1.46 and 47.5±4.84, respectively. Conclusion: The study results and scientific observations regarding reproductive performance suggest that H. wyckii can be considered a new candidate species for aquaculture.

scholarly journals Effect of dietary astaxanthin on reproductive performance, egg quality and larvae of clowfish Amphiprion ocellaris (Cuvier, 1830)

2021 ◽  
Vol 20 (4A) ◽  
pp. 163-172
Author(s):  
Nguyen Thi Nguyet Hue ◽  
Ho Son Lam ◽  
Dao Thi Hong Ngoc ◽  
Dang Tran Tu Tram ◽  
Huynh Minh Sang ◽  
...  
Keyword(s):  
Survival Rate ◽  
Reproductive Performance ◽  
Egg Quality ◽  
Quality Parameters ◽  
Hatching Rate ◽  
Larval Size ◽  
Spawning Period ◽  
Spawning Frequency ◽  
Feeding Trials ◽  
Egg Diameter

This study was designed to evaluate the effects of astaxanthin in broodfish diets on reproductive performance, egg quality and larvae quality parameters of clownfish (Amphirion ocellaris). Five treatments were tested with 5 levels of astaxanthin (Carophyll Pink 10% CWS) of 0, 50, 100, 150 and 200 mg/kg added to the feed. Each treatment was repeated in triplicate and the supplemental feeding trials were arranged for 13 months. The results showed that there were significant differences in hatching rate of egg, malformed rate and survival rate of larvae in 3 days post-hatch (p<0.05) among the feeding trials of astaxanthin supplements. The highest hatching rate of egg and survival rate and the lowest malformed rate of larvae were observed in the treatment that was supplemented with astaxanthin 150 mg/kg feed, respectively 92.14 %; 93.57 % and 0.55 %. However, the astaxanthin supplementary diets did not affect the re-maturation and spawning period, spawning frequency, fecundity, egg diameter and larval size of nemo fish among the treatments. The results also suggested that astaxanthin requirement for clownfish broodstock to improve reproductive performance was 150 mg/kg feed.

scholarly journals REPRODUCTIVE PERFORMANCE OF DOMESTICATED BROODSTOCK OF SILVER PERCH, Bidyanus bidyanus (MITCHELL 1838) AND THE RELATIONSHIP BETWEEN OIL GLOBULE FRAGMENTATION AND EGG QUALITY

2017 ◽  
Vol 12 (2) ◽  
pp. 43 ◽  
Author(s):  
Sulaeman Sulaeman ◽  
Ravi Fotedar
Keyword(s):  
Survival Rate ◽  
Reproductive Performance ◽  
Egg Quality ◽  
Fertilization Rate ◽  
Hatching Rate ◽  
High Fecundity ◽  
Fertilisation Rate ◽  
Significant Difference ◽  
The Relationship

The experiments investigated the reproductive performance of the domesticated broodstock of the silver perch and the relationship between various degrees of oil globule fragmentation and egg quality. Six years old of second generation broodstock (n=3) were evaluated based on the fecundity, fertilisation rate, hatching rate, the degree of oil fragmentation of egg, and the quality of embryos and larvae produced. The fragmentation were grouped into three categories: un-fragmented (cat-1), moderately fragmented (cat-2), and highly fragmented (cat-3). The results showed that the broodstock had a relatively high fecundity (132,400 ± 7,22), fertilization rate (94.27 ± 1.28%), and hatching rates (87.94 ± 1.23%). The survival rate of larvae at 12 days post hatching (dph) in cat-1 (71.3 ± 0.9%) was higher than cat-2 (66.7 ± 0.9%) whereas cat-2 was higher than cat-3 (61.3 ± 0.3%). The eggs was dominated by cat-1 (78.11 ± 2.44%) which was significantly higher than cat-2 (21.26 ± 2.45%) and cat-3 ones (0.40 ± 0.21%). The survival rate of embryo at 20 hours post spawning (hps) and hatching rate of cat-1 (95.33 ± 0.00% and 93.33 ± 0.00%) and cat-2 (90.00 ± 0.00% and 85.00 ± 0.00%) were significantly higher than cat-3 (72.33 ± 1.76% and 60.33 ± 0.00%). The total length (TL) of the larvae of cat-1 and cat-2 (8.44 ± 0.21 mm and 8.35 ± 0.23 mm respectively) were significantly higher than larvae of cat-3 (7.09 ± 0.14 mm). No significant difference was found in the larval deformities among any categories. In conclusion, the reproductive performance of six year-old broodstock silver perch showing acceptable performance and egg categorisation based on oil globule fragmentation can be used as a useful tool to indicate eggs quality of silver perch.

scholarly journals Pengaruh penambahan bahan pengencer sperma terhadap fertilitas spermatozoa ikan lele dumbo (Clarias gariepinus)

2014 ◽  
Vol 1 (1) ◽  
pp. 46
Author(s):  
Prama Hartami ◽  
Muhammad Nur ◽  
Eva Ayuzar
Keyword(s):  
Water Quality ◽  
Survival Rate ◽  
Clarias Gariepinus ◽  
Fertilization Rate ◽  
Coconut Water ◽  
Hatching Rate ◽  
Aceh Province ◽  
Quality Value ◽  
Natural Milk

Penelitian ini dilaksanakan selama 9 hari yang bertempat di Laboratorium Hatcheri dan Teknologi Budidaya Universitas Malikussaleh, Aceh Utara,Propinsi Aceh. Penelitian ini bertujuan untuk mengatahui pengaruh penambahan bahan pengencer sperma terhadap fertilitas spermatozoa ikan lele dumbo dan memperoleh jenis bahan pengencer yang terbaik dalam pemijahan ikan lele dumbo. Penelitian ini di gunakan metode eksperimen laboratorium yang di gunakan pola rancangan acak lengkap (RAL) non faktorial dengan 4 perlakuan 3 ulangan. Bahan uji yang digunakan adalah spermatozoa ikan lele dumbo, Ovaprim, Akuadest, Susu steril, Air kelapa muda, Larutan Infus/ NaCl. Parameter yang di ukur ialah daya fertilitas telur, derajat penetasan telur, kelangsungan hidup benih. Berdasarkan hasil penelitian menunjukkan bahwa penggunaan Aquades sebagai pengencer merupakan bahan yang terbaik dimana fertilitas didapatkan sebesar 98.33 %, derajat penetasan telur sebesar 77.28 %, tingkat kelangsungan hidup larva sebesar 93.44 %. Hasil uji analisis statistik untuk semua parameter uji menunjukkan F hitung > dari F Tabel untuk perlakuan Aquades sebagai bahan pengencer. Sementara itu, hasil pengukuran parameter kualitas air seperti suhu 26,07 – 28,15 0C dan pH air 8,67 – 8,95.This research was done for 9 days in Laboratory of Hatchery and Aquaculture Technology Malikussaleh University, North Aceh, Aceh Province. The aim of this research was to know effect of different sperm solutions for effectiveness fertilization rate of Clarias gariepinus. This research used non factorial completely randomized treatment with three replications on each treatments that using aquadest, natural milk, coconut water and NaCl fisiologis as the sperm solutions. The test observed in this research was fertilization rate, hatching rate, and survival rate. Result showed us that the used of aquadest as the sperm solutions got the best goals which was 98,33 % for ferlilization rate, 77,28 % for hatching rate, and 93,44 % for survival rate. Based on statistical analyze also showed that f count > f table in 99.99 % level of trust for the first treatment with value 29.417 > 7.59. Meanwhile the water quality value that measurable during this research was 26.07 – 28.15 0C and 8.67 – 8.95 for pH.

scholarly journals CONSERVATION OF AN ENDANGERED CARNIVOROUS FISH RITA RITA THROUGH INDUCED BREEDING

2020 ◽  
Vol 2020 (1) ◽  
Author(s):  
S Hayat ◽  
M Ramzan ◽  
M Zafarullaf ◽  
I Ahmad ◽  
Q Ali ◽  
...  
Keyword(s):  
Body Weight ◽  
Water Temperature ◽  
Fertilization Rate ◽  
Hatching Rate ◽  
Breeding Performance ◽  
Carnivorous Fish ◽  
Induced Spawning ◽  
Different Doses ◽  
Successful Production

In present study the Effects of different doses of ovaprim on induced spawning activities of Rita rita were observed with an aim to standardize the dose of ovaprim for successful breeding. In the trial experiment, the females of Rita rita were treated with ovaprim at the rate of 0.5, 0.8 and 1.0ml/kg body weight and males of Rita rita in all the cases were treated at the rate of 0.4ml/kg.  The doses of ovaprim at the rates of 0.5, 0.8, 1.0 ml/kg body weight resulted in 0%, 100% and 100% ovulation respectively. In case of injection of ovaprim at the rate of 0.8ml/kg, the fertilization rate was observed to be about 70% and hatching rate was 58%, which occurred within 20-25 hours after fertilization at water temperature of 270C-290C. In case of injection of ovaprim at the rate of 1.0ml/kg, 18% fertilization rate and 5% hatching rate was observed. The doses of ovaprim at the rates of 0.8ml/kg and 0.4ml/kg body weight for female and male Rita rita respectively was found to be satisfactory for overall breeding performance and the commercially successful production of this fish. Hatchlings from over all experiment were reared up to fingerlings size to about 2 month and were finally stocked into the Chashma lake.

scholarly journals Dose Optimization With Pituitary Gland Hormone For Induced Breeding Of Bata Fish (Labeo Bata)

1970 ◽  
Vol 37 (1) ◽  
pp. 70-77
Author(s):  
MI Miah ◽  
AA Mamun ◽  
MMR Khan ◽  
MM Rahman
Keyword(s):  
Survival Rate ◽  
Pituitary Gland ◽  
Fertilization Rate ◽  
Ovulation Rate ◽  
Optimum Dose ◽  
Dose Optimization ◽  
Hatching Rate ◽  
Brood Rearing ◽  
Labeo Bata ◽  
Average Body

An experiment on induced breeding of bata fish (Labeo bata) was carried out to determine the optimum dose of pituitary gland hormone. The present study consisted of four treatments namely TI, T2, T3, and T4 with three replications of each. Twenty-four pairs of male and twelve pairs of female were selected from the brood rearing ponds and the average body wt of the female and male were 390±10 and 258±9 g respectively. To observe the effective dose for induced breeding, the females were first injected at the rate of 0.5 (T1), 1.0 (T2), 1.5 (T3) and 2.0 (T4) mg PG/kg body wt. while the second doses were performed at the rate of 4.0 (T1), 4.5 (T2), 5.0 (T3), and 5.5 (T4) mg PG/kg body wt. after 6 h from the first dose. On the other hand the males were administered a single dose of 2 mg PG/kg body wt. at the time of second dose of the female. The ovulation rate, fertilization rate, hatching rate and survival rate were determined. Among the four treatments T2 (1.0 mg PG/ kg body wt.) showed the best result in terms of ovulation rate (89%), fertilization rate (84%), hatching rate (85%) and survival rate (84%). The dose of 1.0 mg PG/kg body wt. can be used in induced breeding of bata fish (L. bata) for the development of hatchery propagation. DOI: http://dx.doi.org/10.3329/bjas.v37i1.9871 BJAS 2008; 37(1): 70-77

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