scholarly journals Isolation and selection of bacteria inhibiting Streptococcus agalactiae causing dark-body disease on snakeskin gourami fish (Trichogaster pectoralis)

2021 ◽  
Vol 19 (3) ◽  
pp. 547-555
Author(s):  
Le Thi Anh Hong ◽  
Pham Thi Minh Ngoc ◽  
Duong Khanh ◽  
Vo Van Tuan ◽  
Nguyen Hoang Dung
Keyword(s):  
Streptococcus Agalactiae ◽  
Pathogenic Bacteria ◽  
Survival Rates ◽  
Microbial Pathogens ◽  
Control Treatment ◽  
Fish Ponds ◽  
16S Rrna Sequence ◽  
Experimental Conditions ◽  
Experimental Treatments ◽  
Dark Body

Streptococcus agalactiae is one of the microbial pathogens causing the dark-body disease on snakeskin gourami fish (Trichogaster pectoralis) that affects the growth and quality of fish. This research aimed to isolate and select bacteria inhibiting S. agalactiae which are able to use for controling pathogenic bacteria instead of antibiotics. Fourteen bacteria strains were isolated and screened from healthy fishes, sediment and water samples at fish ponds in Dong Thap province. Among these strains, L7 strain showed the highest inhibition ability with the clear zone diameter was 9,3 mm. The results of the 16S rRNA sequence analysis indicated that the L7 strain belonged to Bacillus subtilis. The experiment to evaluate the inhibition capacity and fish disease control of selected B. subtilis in experimental conditions was conducted by challenging fish with S. agalactiae. Fishes in the control treatment was infected with S. agalactiae at 106 CFU/mL had survival rate 41,7%. The experimental treatments NT1, NT2, NT3 which were treated with B. subtilis at concentrations of 105 CFU/mL, 106 CFU/mL, and 107 CFU/mL gave higher survival rates compared with the non-treated control, with the rates of 60%, 76,7%, and 81,7%, respectively. These results revealed that the isolated B. subtilis is potential used in control dark-body disease on snakeskin gourami fish.

scholarly journals KOMBINASI SIRIH DAN KIPAHIT SEBAGAI IMUNOSTIMULAN TERHADAP PENYAKIT Streptococcosis PADA IKAN NILA (Oreochromis niloticus)

2021 ◽  
Vol 16 (1) ◽  
pp. 39
Author(s):  
Nunak Nafiqoh ◽  
Septyan Andriyanto ◽  
Hessy Novita ◽  
Desy Sugiani ◽  
Taukhid Taukhid
Keyword(s):  
Medicinal Plants ◽  
Streptococcus Agalactiae ◽  
Nile Tilapia ◽  
Pathogenic Bacteria ◽  
Survival Rates ◽  
Challenge Test ◽  
Research Direction ◽  
Future Research ◽  
Control Group ◽  
Active Compounds

Serangan penyakit yang paling banyak ditemui menyerang kegiatan budidaya ikan nila adalah bakteri Streptococcus agalactiae sebagai salah satu agen penyakit Streptococcosis. Peningkatan sistem imun dari ikan yang dibudidayakan merupakan cara yang efektif untuk menanggulangi masalah tersebut. Salah satu fungsi tanaman obat adalah sebagai imunostimulan, di mana sistem imun akan meningkat terutama saat mengalami wabah penyakit ikan. Penelitian ini di tujukan untuk mengetahui efek penggunaan kombinasi ekstrak tanaman sirih dan kipahit dengan dosis yang berbeda terhadap respons imun bawaan dari ikan nila. Dosis yang digunakan adalah 1%, 2%, 4%, dan 8% per kg pakan pemberian pakan dilakukan selama empat minggu, diikuti dengan uji tantang menggunakan bakteri patogen S. agalactiae. Meskipun tidak terlihat perbedaan yang nyata, namun rerata respons imun hematokrit, eritrosit, leukosit, fagositik oksidase, dan lisosim pada kelompok perlakuan menunjukkan nilai yang yang lebih tinggi dibandingkan dengan kontrol. Perbedaan yang signifikan ditunjukkan oleh sintasan, di mana kelompok perlakuan mempunyai nilai sintasan yang lebih tinggi dibandingkan dengan kelompok kontrol. Hasil tersebut diduga disebabkan oleh kandungan bahan aktif yang terdapat dalam tanaman obat. Sehingga penggunaannya mampu bertindak sebagai sebagai upaya pencegahan dari infeksi bakteri patogen S. agalactiae. Perlu diketahui juga apakah kombinasi yang digunakan mampu bertindak untuk terapi (pengobatan).Streptococcosis disease in nile tilapia culture is caused by Streptococcus agalactiae, a bacterium commonly found in freshwater systems. Vaccines and probiotics have been used to prevent the disease outbreaks. However, increasing the innate immune system of cultured fish is more effective in preventing the disease. Medicinal plants have immunostimulant properties, which could be used as an alternative prevention measure against Streptococcosis. This research aimed to determine the effects of betel and kipahit combination as immunostimulant against S. agalactiae in nile tilapia. The combinations consisted of four different doses of 1%, 2%, 4%, and 8% of the plants’ extracts per kg of feed. Feeding experiment was carried out for four weeks, followed by a challenge test using the pathogenic bacteria S. agalactiae. Despite no observable significant differences, haematocrit, erythrocyte, leukocyte, phagocytic oxidase, and lysozyme in the treatment group showed higher values than the control. The treatment groups also had better survival rates than the control group. These results indicate the presence of active compounds within the medicinal plants. In conclusion, supplementation of the plants’ active compounds in feed has the potential as immunostimulant agents in tilapia to prevent Streptococcosis disease caused by pathogenic bacteria S. agalactiae. Determining the capability of this plant combination as a therapeutic agent against S. agalactiaeis an interesting future research direction to be pursued.

scholarly journals Extract from phyllosphere bacteria with antibiofilm and quorum quenching activity to control several fish pathogenic bacteria

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Olivia Nathalia ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
Quorum Quenching ◽  
Indicator Bacteria ◽  
Antibiofilm Activity ◽  
Fish Pathogen ◽  
Phyllosphere Bacteria

Abstract Objective The objective of this research were to screen quorum quenching activity compound from phyllosphere bacteria as well as antibiofilm activity against several fish pathogen bacteria such as Aeromonas hydrophila, Streptococcus agalactiae, and Vibrio harveyi. Results We found eight phyllosphere bacteria isolates with potential quorum quenching activity to inhibit Chromobacterium violaceum as indicator bacteria. Crude extracts (20 mg/mL) showed various antibiofilm activity against fish pathogenic bacteria used in this study. Isolate JB 17B showed the highest activity to inhibit biofilm formation of A. hydrophila and V. harveyi, meanwhile isolate JB 3B showed the highest activity to inhibit biofilm of S. agalactiae. From destruction assay, isolate JB 8F showed the highest activity to disrupt biofilm of A. hydrophila isolate JB 20B showed the highest activity to disrupt biofilm of V. harveyi, isolate JB 17B also showed the highest activity to disrupt biofilm of S. agalactiae.

scholarly journals Influence of Tillage and Daily Manure Application on the Survival of Bacterial Pathogens Indicators in Soil and on Radish

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
James A. Entry ◽  
David L. Bjorneberg ◽  
Sheryl Verwey
Keyword(s):  
Bacterial Adhesion ◽  
Raphanus Sativus ◽  
Pathogenic Bacteria ◽  
Rhizosphere Soil ◽  
Bacterial Colonization ◽  
Sampling Period ◽  
Animal Manure ◽  
Dairy Manure ◽  
Control Treatment ◽  
E Coli

We measuredEscherichia coli, andEnterococcussp. numbers in soil and on fresh radish (Raphanus sativusL.) at 1, 7, 14, 28, 54, and 84 days after the addition of high and low amounts of solid dairy manure in combination with chisel tillage to a 20 cm depth (deep) or roller tillage to a 10 cm depth (shallow). When the high or low amount of solid dairy manure was added to the soil,E. colipopulations in soil were higher in the 54 days following manure addition compared to the control treatment. Dairy manure addition increasedEnterococcussp. in soils compared to the control treatment for the entire 84 days sampling period. At harvest, which was 84 days after application, we did not detectE. coliin radish in rhizosphere soil or on radish roots. Addition of solid dairy manure increasedEnterococcussp. numbers in radish rhizosphere soil and on radish roots. We suggest that fresh animal manure be applied to soil at least 120 days prior to planting to allow die-off of human pathogenic bacteria and reduce the incidence of bacterial adhesion on or bacterial colonization of ready to eat vegetables.

Effect of pasteurization at 76 and 63 °C on the absorption of colostral IgG in calves

2000 ◽  
Vol 67 (4) ◽  
pp. 619-623 ◽  
Author(s):  
JEFF W. TYLER ◽  
JEFF LAKRITZ ◽  
DOUGLAS E. HOSTETLER ◽  
VICTORIA DOUGLAS ◽  
DUSTY M. WEAVER ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Adult Life ◽  
Disease Status ◽  
Heat Treating ◽  
Passive Transfer ◽  
Experimental Conditions ◽  
Biological Behaviour ◽  
Critical Determinant ◽  
Calf Health ◽  
Potential Disadvantage

The timely ingestion and absorption of colostral immunoglobulin is a critical determinant of neonatal calf health. Calves are born without appreciable concentrations of the serum immunoglobulins needed to protect against pathogenic bacteria, viruses and protozoa (Tyler & Parish, 1995). The beneficial effect of passive transfer of colostral immunoglobulin also extends beyond the neonatal period and persists into juvenile and adult life (Robison et al. 1988; Tyler et al. 1998; DeNise et al. 1989). Calves with failure of passive transfer, defined as serum protein < 50 g/l or serum IgG < 10 g/l, have increased mortality risks that persist until 10 weeks of age (Tyler et al. 1998).Several diseases are potentially spread by the ingestion of colostrum, including bovine leukosis and Johne's disease (Perrin & Polack, 1988; Streeter et al. 1995). In one study 22% of latently infected cows were demonstrated to shed Mycobacterium paratuberculosis in their colostrum (Streeter et al. 1995). Optimal programmes to prevent and eradicate these diseases generally include the provision that calves are given colostrum derived from cows of known negative disease status. Pasteurization or heat treatment of colostrum may provide a mechanism whereby calves are provided with protection against neonatal disease without creating undue potential for infection by chronic, economically relevant diseases.Attempts to heat disinfect colostrum are common in goat herds (MacKenzie et al. 1987). Pasteurization has been demonstrated to be effective against the caprine arthritis–encephalomyelitis virus (Adams et al. 1983; MacKenzie et al. 1987). Although Myco. paratuberculosis appears to resist pasteurization, this form of processing has been demonstrated to decrease the likelihood of positive colostral cultures for Myco. paratuberculosis under experimental conditions (Meylan et al. 1996). Sterilizing the milk and colostrum given to calves is a logical and reasonable strategy to prevent transmission of infectious microorganisms. The potential disadvantage of heat treating colostrum is that the immunoglobulins in colostrum may become denatured (Smith & Sherman, 1994). Pasteurization causes only a slight decrease in the colostral concentration of IgG in cattle (Meylan et al. 1996); however, the biological behaviour of these pasteurized immunoglobulins has not been critically examined. Therefore, we cannot be completely confident that immunoglobulin absorption, persistence in serum and biological activity are unchanged by this processing.The goal of this study was to determine the effect of pasteurization at 76 and 63 °C on the absorption of IgG from colostrum. Should these procedures decrease immunoglobulin absorption, the use of pasteurization in disease eradication programmes would require increased efforts to optimize the passive transfer of immunoglobulin.

scholarly journals Efficiency investigation of the use of pyrophyllite in ensiling maize plant

2020 ◽  
Vol 47 (2) ◽  
pp. 109-118
Author(s):  
Milan Adamović ◽  
Mirjana Stojanović ◽  
Muhamed Harbinja ◽  
Marijana Maslovarić ◽  
Aleksandra Bočarov-Stančić ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Volatile Fatty Acids ◽  
Ph Value ◽  
Fine Sand ◽  
Maize Plant ◽  
Control Treatment ◽  
Green Mass ◽  
The Moment ◽  
Experimental Treatments ◽  
Air Passage

The paper presents the results of testing the effect of pyrophyllite shale (pyrophyllite) on the quality of maize plant silage, primarily on the production of organic acids, pH value, quality assessment and microbiological safety. The ensiling was done in plastic containers which allowed the storage of 10 kg of chopped green mass. Tested pyrophyllite doses were: 0% in the control treatment (I), 0.5 and 1.0% in experimental treatments (II and III, respectively). Granulated pyrophyllite (100 µm), originating from Parsovići, Konjic site, AD Harbi Ltd., Sarajevo, Bosnia and Herzegovina, was manually incorporated. The chopped green mass of the maize plant came from FAO 600 hybrids. The green mass containing 37.17% of dry matter (final waxy ripening phase) was compressed in the same manner in all three treatments during the filling of the vessels. The containers were then covered with nylon foil (0.2 mm) above which a layer of fine sand (approx. 5 cm) was placed in toward the silage protection from air passage. The silages were opened after 7 weeks and organoleptic, chemical and microbiological analyses were performed. The organoleptic properties of silages (colour and odour) were better in silages containing 0.5 and 1.0% pyrophyllite. The silage temperature at the moment of opening of the containers was lower in the treatments with 0.5 and 1.0% added pyrophyllite (13.7 and 13.2 °C, respectively) while in the control treatment it was 14.6 °C. The addition of pyrophyllite to silage affected the production of volatile fatty acids (p ≤ 0.05). The highest amount of lactic acid was found in the silage sample without the addition of pyrophyllite, and acetic acid in the silage treated with 0.5 and 1.0% of pyrophyllite. The lowest amount of butyric acid was determined in the silage with the addition of 1% pyrophyllite. Based on the content and interrelationship of lactic, acetic and butyric acid, as well as the pH values, all three silages were rated as the highest (I) class. The number of aerobic mesophilic bacteria, as well as the number of yeasts, was lower in the silages with the addition of 0.5 and 1.0% pyrophyllite. In future, particular attention should be paid on the possibility of pyrophyllite enrichment (e.g. with nitrogen) and more appropriate physical formulation (e.g. granules) that would allow more efficient practical application.

Effects of Five Experimental Treatments on Stuttering

1979 ◽  
Vol 22 (1) ◽  
pp. 132-146 ◽  
Author(s):  
Richard Martin ◽  
Samuel K. Haroldson
Keyword(s):  
Auditory Feedback ◽  
Change Score ◽  
Treatment Change ◽  
Noise Condition ◽  
Experimental Conditions ◽  
Delayed Auditory Feedback ◽  
Change Scores ◽  
Time Out ◽  
Experimental Treatments

Twenty adult stutterers were exposed to each of five experimental treatments: time-out, noise, delayed auditory feedback (DAF), “wrong” and metronome. In each session a subject spoke for 20 minutes without treatment (baserate) followed by 30 minutes in one of the five experimental conditions. Before the five treatment sessions, subjects accomplished three pre-experimental tasks: expectancy, changeability, and adaptation tasks. Percent stuttering decreased significantly in all conditions, and stuttering duration reduced significantly in all but the noise condition. The amount of reduction in percent stuttering from baserate to treatment (change score) in time-out was positively related to the change scores in DAF and metronome. Change scores in metronome were positively related to change scores in time-out and “wrong.” Percent stuttering change scores in noise, DAF, and “wrong” were essentially unrelated. Stuttering duration change scores were related only for the time-out and DAF, and metronome and DAF conditions. In general, the pre-experimental expectancy, changeability, and adaptation scores were unrelated to change scores in any of the experimental conditions. Words spoken per minute did not change significantly from baserate to treatment for any experimental condition except time-out.

THE STATUS OF RECYCLING OF ORGANIC WASTE ON THE POULTRY FARM «MIKHNEVSKAYA»

2019 ◽  
Vol 1 (2) ◽  
pp. 198-204
Author(s):  
Yu. M. Subbotina ◽  
Keyword(s):  
Pathogenic Bacteria ◽  
Aquatic Vegetation ◽  
Experimental Studies ◽  
Sewage Water ◽  
Poultry Farm ◽  
Fish Ponds ◽  
Seed Material ◽  
Higher Aquatic Vegetation ◽  
The Status ◽  
Poultry Wastewater

The article deals with the problem of recycling in poultry farm Mikhnevskaya. The work program provided for the continuation of studies on the development of the system of pond preparation of sewage water in biological ponds of purification. As one of the options, the systems of BOCS ponds (biological oxidation contact stabilization ponds) and ponds with higher aquatic vegetation, as a system of posttreatment of wastewater, as well as the use of fish ponds were proposed. The development of devices that provide deeutrophication of decontaminated wastewater in the BOCS ponds was started. As measures that contributed to the improvement of water purification to be discharged into the environment, an attempt was made to introduce at the final stages of purification of the algobacterial complex, higher aquatic vegetation, and fish seed material. A survey of the poultry farm was conducted; the conditions of wastewater formation of the Mikhnevskaya poultry farm of Stupinsky district of the Moscow region were studied. Model experiments were carried out at the Mikhnevskaya poultry farm in laboratory and semi-production conditions, directly in the laboratory of the Institute at the facility. In the course of experimental studies it was found that in the ponds under the influence of algological complex of microalgae was the process of purification and disinfection of poultry wastewater. The total death of pathogenic bacteria of the E. coli group occurred on 6-11 days after the introduction of microalgae into the water of the algal pond, which does not contradict the existing developments. At the same time, there was still an increase in fish seed material, which was sufficiently provided with food and by the autumn reached a mass of 410 grams.

scholarly journals In Vitro Evaluation of Probiotic Properties of Lactic Acid Bacteria Isolated from Some Traditionally Fermented Ethiopian Food Products

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Guesh Mulaw ◽  
Tesfaye Sisay Tessema ◽  
Diriba Muleta ◽  
Anteneh Tesfaye
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Survival Rates ◽  
Lactobacillus Paracasei ◽  
Probiotic Properties ◽  
Sequence Comparisons

Probiotics are live microorganisms which when consumed in large number together with a food promote the health of the consumer. The aim of this study was to evaluate in vitro probiotic properties of lactic acid bacteria (LAB) isolated from traditional Ethiopian fermented Teff injera dough, Ergo, and Kocho products. A total of 90 LAB were isolated, of which 4 (4.44%) isolates showed 45.35–97.11% and 38.40–90.49% survival rates at pH values (2, 2.5, and 3) for 3 and 6 h, in that order. The four acid-tolerant isolates were found tolerant to 0.3% bile salt for 24 h with 91.37 to 97.22% rate of survival. The acid-and-bile salt-tolerant LAB isolates were found inhibiting some food-borne test pathogenic bacteria to varying degrees. All acid-and-bile-tolerant isolates displayed varying sensitivity to different antibiotics. The in vitro adherence to stainless steel plates of the 4 screened probiotic LAB isolates were ranged from 32.75 to 36.30% adhesion rate. The four efficient probiotic LAB isolates that belonged to Lactobacillus species were identified to the strain level using 16S rDNA gene sequence comparisons and, namely, were Lactobacillus plantarum strain CIP 103151, Lactobacillus paracasei subsp. tolerans strain NBRC 15906, Lactobacillus paracasei strain NBRC 15889, and Lactobacillus plantarum strain JCM 1149. The four Lactobacillus strains were found to be potentially useful to produce probiotic products.

The influence of some physical characteristics of the milking machine on the rate of new mastitis infections

1973 ◽  
Vol 40 (1) ◽  
pp. 117-129 ◽  
Author(s):  
C. C. Thiel ◽  
Carol L. Cousins ◽  
D. R. Westgarth ◽  
F. K. Neave
Keyword(s):  
Short Duration ◽  
Streptococcus Agalactiae ◽  
The Other ◽  
Vacuum Fluctuations ◽  
Infection Rates ◽  
Experimental Conditions ◽  
Different Types ◽  
Before And After ◽  
Milking Machine ◽  
Do So

SummaryLarge differences in new infection rates occurred with half-udder milking machines in which one half of the cluster operated substantially free from vacuum fluctuations while the other half was subjected to various combinations of different types of fluctuation in vacuum. To increase the rate of new infection all teats were contaminated before and after each milking by immersion in a suspension of Streptococcus agalactiae and Str. dysgalactiae. A series of short-duration trials indicated that neither irregular nor cyclic fluctuations in vacuum acting alone were able to increase new infection rate. However, either the Nyhan and Cowhig irregular fluctuation, or a variant of it in combination with cyclic fluctuations in vacuum, was able to do so under the particular experimental conditions used.

scholarly journals in vivo gene expression and the adaptive response: from pathogenesis to vaccines and antimicrobials

2000 ◽  
Vol 355 (1397) ◽  
pp. 633-642 ◽  
Author(s):  
Douglas M. Heithoff ◽  
Robert L. Sinsheimer ◽  
David A. Low ◽  
Michael J. Mahan
Keyword(s):  
Pathogenic Bacteria ◽  
Acid Stress ◽  
Infection Process ◽  
Bacterial Virulence ◽  
Microbial Pathogens ◽  
Virulence Determinants ◽  
Preferential Expression ◽  
Successful Infection ◽  
Dna Adenine Methylase

Microbial pathogens possess a repertoire of virulence determinants that each make unique contributions to fitness during infection. Analysis of these in vivo –expressed functions reveals the biology of the infection process, encompassing the bacterial infection strategies and the host ecological and environmental retaliatory strategies designed to combat them (e.g. thermal, osmotic, oxygen, nutrient and acid stress). Many of the bacterial virulence functions that contribute to a successful infection are normally only expressed during infection. A genetic approach was used to isolate mutants that ectopically expressed many of these functions in a laboratory setting. Lack of DNA adenine methylase (Dam) in Salmonella typhimurium abolishes the preferential expression of many bacterial virulence genes in host tissues. Dam − Salmonella were proficient in colonization of mucosal sites but were defective in colonization of deeper tissue sites. Additionally, Dam − mutants were totally avirulent and effective as live vaccines against murine typhoid fever. Since dam is highly conserved in many pathogenic bacteria that cause significant morbidity and mortality worldwide, Dams are potentially excellent targets for both vaccines and antimicrobials.

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