scholarly journals Somatic embryogenesis in the commercial papaya hybrid UENF/Caliman 01 relying on plantlet production from sexed adult hermaphrodite donor plants

2019 ◽  
Vol 91 (3) ◽  
Author(s):  
DRIELLY S. GOUVEA ◽  
KRISTHIANO CHAGAS ◽  
JAMILE L.D. CIPRIANO ◽  
JOSÉ CARLOS LOPES ◽  
EDILSON R. SCHMILDT ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Plantlet Production ◽  
Adult Hermaphrodite

scholarly journals Haploid Plantlet Production through Somatic Embryogenesis in Anther-Derived Callus ofBupleurum falcatum

2004 ◽  
Vol 7 (2) ◽  
pp. 204-211 ◽  
Author(s):  
Tae-Kwon Shon ◽  
Sang-Kuk Kim ◽  
Daniel Acquah ◽  
Sang-Chul Lee
Keyword(s):  
Somatic Embryogenesis ◽  
Plantlet Production

scholarly journals Somatic Embryogenesis and Regeneration from Cotyledon Explants of Six Squash Cultivars

HortScience ◽  
1995 ◽  
Vol 30 (6) ◽  
pp. 1295-1297 ◽  
Author(s):  
Carol Gonsalves ◽  
Baodi Xue ◽  
Dennis Gonsalves
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Induction Medium ◽  
Dichlorophenoxyacetic Acid ◽  
Plantlet Production ◽  
Summer Squash ◽  
Napthaleneacetic Acid ◽  
Relative Production ◽  
2,4 Dichlorophenoxyacetic Acid

Six summer squash (Cucurbita pepo L.) cultivars were regenerated via somatic embryogenesis using cotyledons excised from germinated or nongerminated seeds. Genotypes included were zucchini, commercial F1 hybrids, `President', `Seneca Zucchini', `Jade'; the noncommercial inbred line `Caserta Inbred 557311'; and two yellow squash hybrids `Dixie' and `Seneca Butterbar'. Somatic embryogenesis was initiated in induction medium containing 22.62 μm 2, 4-D, and embryos were germinated in maturation medium containing 0.27 μm NAA and 0.23 μm kinetin. Plants were elongated and rooted on basal medium without hormones. All media contained carbenicillin at 500 mg·liter–1. Sixty-one percent of the `Seneca Butterbar' cotyledons produced somatic embryos when kept on induction medium for 10 weeks. Overall, 7% of the initial explants produced plantlets, and regeneration efficiency was calculated as 0.3 plantlets per initial explant. The relative production of plants from cotyledons that were kept on induction medium for different time periods were determined for `Caserta Inbred 557311' and `Seneca Zucchini'. All cotyledons produced somatic embryos after 11 to 17 weeks on induction medium. However, plantlet production was optimal with explants kept on induction medium for 13 weeks for `Seneca Zucchini' and for 15 weeks for `Caserta Inbred 557311', producing an average of 4.5 and 9.3 plants per explant, respectively, from 90% to 70% of the explants. We recovered plants from all six cultivars; thus, our regeneration protocol may be applicable to other genotypes. The high percentage of regenerants obtained indicates that the regeneration method is efficient enough to be adapted successfully to squash transformation experiments. Chemical names used: α-carboxybenzylpenicillin (carbenicillin); 2,4-dichlorophenoxyacetic acid (2,4-D); 6-furfurylaminopurine (kinetin); α-napthaleneacetic acid (NAA).

scholarly journals 028 SOMATIC EMBRYOGENESIS IN QUERCUS SPECIES

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 431e-431
Author(s):  
Sudeep Vyapari ◽  
Houchang Khatamian
Keyword(s):  
Somatic Embryogenesis ◽  
Callus Induction ◽  
Somatic Embryos ◽  
Zygotic Embryo ◽  
Shoot Formation ◽  
Chilling Temperature ◽  
Air Drying ◽  
Plantlet Production ◽  
Quercus Species ◽  
Quercus Palustris

Somatic embryogenesis was successfully achieved in chinkapin oak (Quercus muehlenbergii Engelm.) and pin oak (Quercus palustris Muenchh.) when surface disinfested zygotic embryo explants were cultured on MS or WPM containing BA or kinetin (1.0 or 2.0 mg 1-1) plus IBA (1.0 mg 1-1). Immature embryos resulted in greater callus induction than the mature ones. Two weeks of dark, proved to be superior to 4 weeks or no dark in callus induction. Somatic embryos of pin oak distinctly showed globular, heart and cotyledonary stages. Maturation and germination of pin oak somatic embryos was done in growth regulator free WPM by increasing levels of agar (7 - 15 g 1-1). Somatic embryos cultured at various levels of agar were then maintained in incubator under standard conditions, desiccated by air-drying or subjected to chilling temperature for 4 weeks to enhance germination of somatic embryos. Root or shoot formation was observed in some cultures, and medium with 9 g 1-1 agar induced plantlet production in 7% of the cultures.

Somatic embryogenesis in conifers

1991 ◽  
Vol 69 (9) ◽  
pp. 1873-1899 ◽  
Author(s):  
T. E. Tautorus ◽  
L. C. Fowke ◽  
D. I. Dunstan
Keyword(s):  
Somatic Embryogenesis ◽  
Picea Glauca ◽  
Somatic Embryos ◽  
Zygotic Embryos ◽  
Zygotic Embryogenesis ◽  
Direct Gene Transfer ◽  
Plantlet Production ◽  
Substantial Progress ◽  
Liquid Suspensions

Substantial progress has been made towards the development of systems for in vitro embryogenesis in conifers. Since the first report of somatic embryogenesis from zygotic embryos of Picea abies in 1985, cultured explants of at least 18 different coniferous species have been induced to produce somatic embryos. Somatic embryos have been cryopreserved, grown in liquid suspensions, and matured into plants. In addition, plantlets have been regenerated from protoplasts isolated from embryogenic suspensions of Picea glauca and Larix ×eurolepis, permitting studies into direct gene transfer and somatic hybridization. Currently however, it is only possible to obtain somatic embryogenesis from embryonic and juvenile explants. Furthermore, for most species the efficiency of plantlet production from somatic embryos is poor and remains a problem for the commercial utilization of this technology. Biochemical, cytological, and physiological studies of conifer somatic embryogenesis have resulted in improved knowledge concerning the origin of somatic embryos, storage product accumulation during embryo development, and similarities with zygotic embryos. Furthermore, the technique of indirect immunofluorescence microscopy has permitted investigations of the cytoskeleton in conifer cells and protoplasts, providing insights into cell division and morphogenesis. In this review, emphasis is placed on the more fundamental aspects of conifer somatic embryogenesis. Where possible, comparisons between zygotic and somatic embryogenesis are made. Key words: somatic embryogenesis, zygotic embryogenesis, conifers, review.

Plantlet production through high frequency somatic embryogenesis in long term cultures ofEucalyptus citriodora

1989 ◽  
Vol 8 (1) ◽  
pp. 41-43 ◽  
Author(s):  
E. M. Muralidharan ◽  
P. K. Gupta ◽  
A. F. Mascarenhas
Keyword(s):  
Somatic Embryogenesis ◽  
High Frequency ◽  
Plantlet Production

Somatic embryogenesis of Cyclamen persicum in liquid medium

1995 ◽  
Vol 94 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Marc Kreuger ◽  
Erik Postma ◽  
Yvon Brouwer ◽  
Gerrit-Jan van Holst
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Cyclamen Persicum

Somatic Embryogenesis and In vitro Plant Regeneration in Vigna trilobata (L.) Verd. - A Potential Pasture Legume

1970 ◽  
Vol 19 (1) ◽  
pp. 89-99
Author(s):  
K. Choudhary ◽  
M. Singh ◽  
M. S. Rathore ◽  
N. S. Shekhawat
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Long Term Study ◽  
Continuous Application ◽  
First Time ◽  
Pasture Legume

This long term study demonstrates for the first time that it is possible to propagate embryogenic Vigna trilobata and to subsequently initiate the differentiation of embryos into complete plantlets. Initiation of callus was possible on 2,4-D. Somatic embryos differentiated on modified MS basal nutrient medium with 1.0 mg/l  of 2,4-D and 0.5 mg/l  of Kn. Sustained cell division resulted in globular and heart shape stages of somatic embryos. Transfer of embryos on to a fresh modified MS basal medium with 0.5 mg/l of Kn and 0.5 mg/l of GA3 helped them to attain maturation and germination. However, the propagation of cells, as well as the differentiation of embryos, were inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking these growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in Vigna species are discussed. Key words: Pasture  legume, Vigna trilobata, Globular, Heart shape, somatic embryogenesis D.O.I. 10.3329/ptcb.v19i1.4990 Plant Tissue Cult. & Biotech. 19(1): 89-99, 2009 (June)

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