In vitro germination of Abricó-de-Macaco (Couroupita guianensis aubl.)zygotic embryos in different culture media and light spectra

ABSTRACT: Couroupita guianensis Aubl. is an Amazonian forest species with important medicinal and ornamental value. This study evaluated the effect of different culture media and light spectra on the in vitro germination and development of the zygotic embryos of C. guianensis. The culture media, MS and WPM, were evaluated without the addition of plant growth regulators and were associated with four LED light spectra: white (CW), 70% red + 30% blue (R2B), 100% red (R), and 100% blue (B). One hundred percent of the seeds successfully underwent in vitro germination, and the culture media did not interfere with embryo development. In addition to this, the different light spectra induced in vitro morphogenesis and R2B treatment significantly promoted the production of secondary roots. This effect may aid in the rooting and acclimatization of seedlings of this species.

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Effect of plant growth regulators on in vitro germination of coffee zygotic embryos

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb11.2794 ◽

2011 ◽

Vol 10 (82) ◽

Author(s):

J. Emanuel de A. Bojórquez-Quintal

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Zygotic Embryos ◽

In Vitro Germination

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Propagação in vitro de Zingiber spectabile

Ornamental Horticulture ◽

10.14295/oh.v23i3.1035 ◽

2017 ◽

Vol 23 (3) ◽

pp. 270

Author(s):

Michele Valquíria Dos Reis ◽

Fernanda Carlota Nery ◽

Débora De Oliveira Prudente ◽

Patricia Duarte de Oliveira Paiva ◽

Renato Paiva ◽

...

Keyword(s):

Plant Growth ◽

Activated Charcoal ◽

In Vitro Propagation ◽

Culture Media ◽

In Vitro Germination ◽

Phase Pattern ◽

High Germination ◽

Length Width ◽

Ornamental Species

Zingiber spectabile is a tropical ornamental species with difficulties to obtain efficient propagation system. Thus, this study aimed to assess the in vitro propagation of Zingiber spectabile. Seed characterization was determined by measuring length, width and thickness, the weight of 1000 seeds and imbibition curve. In vitro germination of seeds was at constant (25 °C) or alternating temperatures (20-30 ºC). For optimization of in vitro multiplication, different concentrations of activated charcoal (0.0, 0.1 and 0.3%) and sucrose (0.0, 0.1, 0.3, 0.5 and 0.7 M) were evaluated. Plantlets were inoculated in flasks with different sealing systems (PVC covers with or without filters at the center) and culture media (MS or WPM). The plants were acclimatized in Plantmax® substrate. Seeds were of 6.06 mm length, 3.22 mm wide and 2.83 mm thick. The weight of 1,000 seeds corresponded to 46.4 g. The seed imbibition curve approaches to a tree phase pattern. Alternating temperatures induced high germination rates (68%). The addition of 0.3% activated charcoal provided higher root growth and plants with smaller number of senescent leaves. The best plant growth was obtained by the use of 0.1 M sucrose. All acclimatized plants survived (100%). The results demonstrate that Z. spectabile respond well to in vitro propagation.

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In vitro germination of zygotic embryos of hybrid BRS Manicoré (E. guineensis X E. oleifera)

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201620140685 ◽

2016 ◽

Vol 88 (3 suppl) ◽

pp. 1841-1850

Author(s):

KEILA A.P. BONETTI ◽

MARGUERITE QUOIRIN ◽

REGINA C. QUISEN ◽

SUELEN C.S. LIMA

Keyword(s):

Culture Medium ◽

Culture Media ◽

Germination Rate ◽

Phosphate Concentration ◽

Zygotic Embryos ◽

In Vitro Germination ◽

N6 Medium ◽

Whole Plants ◽

Tetrazolium Test

ABSTRACT The interspecific oil palm hybrid BRS Manicoré (E. guineensis x E. oleifera) has superior agronomic characteristics. However, the germination rate is low (30%) and the process is slow when the seeds are sown in a conventional form. The purpose of this study was to optimize the in vitro germination of zygotic embryos of this hybrid comparing seed lots. The viability of zygotic embryos was evaluated by the tetrazolium test (0.075%) for 4 h. The embryos were cultured on MS and Y3 culture media, with and without the addition of NaH2PO4, as well as on MS, MS1/2 and N6 medium. In MS medium containing NaH2PO4, the germination rate was increased from 40 to 70% in comparison with the medium without sodium phosphate. The comparison between the culture media MS, MS 1/2, N6 and Y3 showed that 75% of zygotic embryos cultured in the Y3 medium formed whole plants (with roots and shoots defined), a higher percentage than embryos cultured on MS, MS 1/2 and N6 media (46, 35 and 17% respectively). In the same Y3 culture medium, the embryos were larger (36% ≥ 2 cm and 30% ≥ 5 cm) than in the other media. Results obtained by the tetrazolium test were similar to those of germination, showing the effect of the genotype of each seed lot. For the germination and development of plantlets it is essential to add NaH2PO4 to a culture medium containing no phosphate or with a low phosphate concentration.

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Effect of culture media, plant growth regulators and genotypes on growth and developmental stages of oil palm (Elaeis guineensis Jacq.) zygotic embryos

Indian Journal of Agricultural Research ◽

10.18805/a-5129 ◽

2019 ◽

Author(s):

D.S. Sparjanbabu ◽

P. Naveen Kumar ◽

M.S.R. Krishna ◽

D. Ramajayam ◽

B. Kalyana Babu ◽

...

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Seed Production ◽

Growth Regulators ◽

Oil Palm ◽

Elaeis Guineensis ◽

Culture Media ◽

Zygotic Embryos ◽

Elaeis Guineensis Jacq

Oil palm (Elaeis guineensis Jacq.) has become the largest traded vegetable oil in the world due to its innumerable uses and its huge economic importance. Which increases the requirement of quality seedling but the conventional seed production limits the commercial seed production with its long germination period and low germination rate. In this context in vitro germination of zygotic embryo could be a promising alternative. Hence in this study the effect of culturing media, plant growth regulators and genotypes were assessed on in vitro regeneration and plantlet development of oil palm zygotic embryos. Zygotic embryos of four elite genotypes were cultured on MS, N6 and Y3 media, with or without plant growth regulators (NAA, BA and GA3-0.2mg/lt). There was no effect of culture media, plant growth regulators and genotypes on germination of zygotic embryos. Whereas on the growth and development of in vitro plantlets, there was a significant effect of culture media and genotypes. Where culture media N6 performs well and Y3 has shown more abnormality. In case of genotypes, genotypes G1 and G2 shown better performance when compared to genotypes G3 and G4. The effect of plant growth regulators on germination and plant growth was non significant.

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Influence of Light Conditions and Medium Composition on Morphophysiological Characteristics of Stevia rebaudiana Bertoni In Vitro and In Vivo

Horticulturae ◽

10.3390/horticulturae7070195 ◽

2021 ◽

Vol 7 (7) ◽

pp. 195

Author(s):

Alla A. Shulgina ◽

Elena A. Kalashnikova ◽

Ivan G. Tarakanov ◽

Rima N. Kirakosyan ◽

Mikhail Yu. Cherednichenko ◽

...

Keyword(s):

Culture Media ◽

Stevia Rebaudiana ◽

Adventitious Shoot ◽

Optimal Growth ◽

Medium Composition ◽

Light Spectra ◽

Stevia Rebaudiana Bertoni ◽

Multiplication Coefficient

We investigated the influence of different conditions (light composition and plant growth regulators (PGRs) in culture media) on the morphophysiological parameters of Stevia rebaudiana Bertoni in vitro and in vivo. Both PGRs and the light spectra applied were found to significantly affect plant morphogenesis. During the micropropagation stage of S. rebaudiana, optimal growth, with a multiplication coefficient of 15, was obtained in an MS culture medium containing 2,4-epibrassinolide (Epin) and indole-3-acetic acid (IAA) at concentrations of 0.1 and 0.5 mg L−1, respectively. During the rooting stage, we found that the addition of 0.5 mg L−1 hydroxycinnamic acid (Zircon) to the MS medium led to an optimal root formation frequency of 85% and resulted in the formation of strong plants with well-developed leaf blades. Cultivation on media containing 0.1 mg L−1 Epin and 0.5 mg L−1 IAA and receiving coherent light irradiation on a weekly basis resulted in a 100% increase in the multiplication coefficient, better adventitious shoot growth, and a 33% increase in the number of leaves. S. rebaudiana microshoots, cultured on MS media containing 1.0 mg L−1 6-benzylaminopurine (BAP) and 0.5 mg L−1 IAA with red monochrome light treatments, increased the multiplication coefficient by 30% compared with controls (white light, media without PGRs).

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Control of browning during seed germination of Meizotropis pellita: a rare and an endangered plant

Research Journal of Biotechnology ◽

10.25303/168rjbt11821 ◽

2021 ◽

Vol 16 (8) ◽

pp. 118-120

Author(s):

Renu Chandola ◽

Anshulika Upadhyaya

Keyword(s):

Seed Germination ◽

Culture Media ◽

Light Condition ◽

Endangered Plant ◽

In Vitro Germination ◽

Light Conditions ◽

Low Intensity ◽

Browning Reaction ◽

Threatened Plant Species

Meizotropis pellita is a rare, endangered and threatened plant species found in Patwadanger forest. In nature this species grows from rootstock in precise and sensitive habitats. However, phenolic compounds provoke browning reaction which is responsible for lethal browning in plant tissue culture and hindering in vitro germination. The aim of this study was to minimize the effect of phenolic compound during seed germination. We evaluate different antibrowning treatments in Meizotropis pellita seeds. The seeds were pretreated in Polyvinylpyrrolidone and then treated seeds were cultured on MS media and sterilized seeds were directly inoculated on PVP containing MS media. These culture media incubate under dark and light conditions at different durations respectively. Our results showed that addition of antibrowning agent, PVP inhibit onset of browning. Dark and light condition also played a crucial role on onset of browning. Delayed and low intensity of browning were observed in case of seeds that were inoculated on MS media containing PVP and were kept in dark. The present research offers a positive in vitro seed germination protocol for Meizotropis pellita conservation.

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