scholarly journals Isolation and screening of chromium resistant bacteria from industrial waste for bioremediation purposes

2023 ◽  
Vol 83 ◽  
Author(s):  
Kalsoom ◽  
Afshan Batool ◽  
Ghufranud Din ◽  
Salah Ud Din ◽  
Johar Jamil ◽  
...  
Keyword(s):  
Industrial Waste ◽  
Heavy Metal Contamination ◽  
Industrial Effluent ◽  
Toxic Metal ◽  
Maximum Growth ◽  
Major Constituent ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Toxic Heavy Metals ◽  
16S Rrna Sequence

Abstract Chromium (VI) a highly toxic metal, a major constituent of industrial waste. It is continuously release in soil and water, causes environmental and health related issues, which is increasing public concern in developing countries like Pakistan. The basic aim of this study was isolation and screening of chromium resistant bacteria from industrial waste collected from Korangi and Lyari, Karachi (24˚52ʹ46.0ʺN 66˚59ʹ25.7ʺE and 24˚48ʹ37.5ʺN 67˚06ʹ52.6ʺE). Among total of 53 isolated strains, seven bacterial strains were selected through selective enrichment and identified on the basis of morphological and biochemical characteristics. These strains were designated as S11, S13, S17, S18, S30, S35 and S48, resistance was determined against varying concentrations of chromium (100-1500 mg/l). Two bacterial strains S35 and S48 showed maximum resistance to chromium (1600 mg/l). Bacterial strains S35 and S48 were identified through 16S rRNA sequence and showed 99% similarity to Bacillus paranthracis and Bacillus paramycoides. Furthermore, growth condition including temperature and pH were optimized for both bacterial strains, showed maximum growth at temperature 30ºC and at optimum pH 7.5 and 6.5 respectively. It is concluded that indigenous bacterial strains isolated from metal contaminated industrial effluent use their innate ability to transform toxic heavy metals to less or nontoxic form and can offer an effective tool for monitoring heavy metal contamination in the environment.

scholarly journals Bioremoval of Different Heavy Metals in Industrial Effluent by the Resistant Fungal Strain Aspergillus niger

2021 ◽  
Vol 20 (4) ◽  
Author(s):  
K. J. Naveen Kumar ◽  
J. Prakash
Keyword(s):  
Heavy Metals ◽  
Aspergillus Niger ◽  
Metal Binding ◽  
Metal Removal ◽  
Low Cost ◽  
Heavy Metal Removal ◽  
Industrial Effluent ◽  
Toxic Metal ◽  
Differential Pulse ◽  
Toxic Heavy Metals

Developing countries are increasingly concerned with pollution due to toxic heavy metals in the environment. Unlike most organic pollutants which can be destroyed, toxic metal ions released into the environment often persist indefinitely circulating and eventually accumulating throughout the food chain thus posing a serious threat to mankind. The use of biological materials for heavy metal removal or recovery has gained importance in recent years due to their good performance and low cost. Among the various sources, both live and inactivated biomass of organisms exhibits interesting metal binding capacities. Their complex cell walls contain high content of functional groups like amino, amide, hydroxyl, carboxyl, and phosphate which have been implicated in metals binding. In the present study, Aspergillus niger was used to analyze the metal uptake from an aqueous solution. The determination of Cu+2, Pb+2, Cd+2, Zn+2, Co-2 and Ni+2 in samples was carried out by differential Pulse Anodic Voltammetry (DPASV) and the Voltammograms. Production of oxalic acid was carried out by submerged fermentation. The organism used in the present study has the ideal properties to sequester toxic metals and grow faster.

Deciphering the Multi-Dimensional Abilities of Indigenous Bacteria Enterobacter Cloacae Isolated from Arsenic Contaminated Industrial Sites

2021 ◽  
Author(s):  
Reeta Bhati ◽  
Smitha Mony Sreedha ◽  
Rajni Singh
Keyword(s):  
Defense Mechanisms ◽  
Toxic Metal ◽  
Enterobacter Cloacae ◽  
Maximum Growth ◽  
Contaminated Site ◽  
Bacterial Strains ◽  
High Concentration ◽  
Industrial Sites ◽  
Zinc Cadmium ◽  
Cadmium Selenium

Abstract Arsenic (As) is a quintessential toxic metalloid and it has been classified as Group 1 human carcinogen. The evolution of arsenic defense mechanisms due to the omnipresent nature of arsenic has resulted in its alteration to less toxic forms. The present study deals with the isolation of arsenic remediating microbial strains from soil samples and their integration into bioremediation strategy. From the metal contaminated site, 118 different bacterial strains were isolated from heavy metal contaminated site. Twenty-five strains were tolerant to arsenic and one bacterial strain Enterobacter cloacae (RSC3) demonstrated maximum growth at high concentration of arsenate (6000ppm). The cell growth kinetics of RSC3revealed the specific growth rate (µ) to be 0.55 h-1. The The bacteria hosts arsC gene in the genome involved in the reduction of arsenate to arsenite. AAS, SEM, TEM and EDX studies confirmed the arsenate transportation and efflux of arsenic by the bacteria. Furthermore, the strain showed multi-resistance to other heavy metals like zinc, cadmium, selenium and nickel and several antibiotics indicating its application for facilitating bioremediation of toxic metal contaminated sites.

scholarly journals Characterization of multiple metal resistant Bacillus licheniformis and its potential use in arsenic contaminated industrial wastewater

2021 ◽  
Vol 11 (4) ◽  
Author(s):  
Shahid Sher ◽  
Sikander Sultan ◽  
Abdul Rehman
Keyword(s):  
Bacillus Licheniformis ◽  
Toxic Metal ◽  
Growth Conditions ◽  
Oxidation Potential ◽  
Optimum Growth ◽  
Toxic Heavy Metals ◽  
16S Rrna Sequence ◽  
Optimum Growth Temperature ◽  
Potential Use

AbstractIn the present study, the arsenic bioremediation ability of Bacillus licheniformis (dubbed as A6) was determined. The strain was isolated from metal polluted wastewater and was identified on the basis of 16S rRNA sequence homology with accession number of KX 785,171. The bacterium showed resistance against multiple toxic heavy metals, and MIC against arsenic was 3000 µg/ml. Resistance of the bacterium against other toxic metal ions was 3000 µg/ml (Cr), 50 µg/ml (Hg), 1000 µg/ml (Mn), 4000 µg/ml (Se), 500 µg/ml (Pb), 100 µg/ml (Co), 70 µg/ml (Cd) and 100 µg/ml (Zn). The optimum growth temperature was 37 °C while pH was 7. The strain also showed resistance against commonly used antibiotics except ceftriaxone 30 µg and amoxicillin with clavulanic acid (2:1) 3 µg. B. licheniformis could oxidize arsenite into arsenate 86 and 98% after 48 and 96 h from the medium at optimum growth conditions. Due to its high oxidation potential, B. licheniformis can be used in the biological treatment of wastewater containing arsenic.

scholarly journals Study of an arsenic metabolizing bacteria from arsenic contaminated soil of Chandpur district, Bangladesh

2019 ◽  
Vol 8 (1) ◽  
pp. 57-65
Author(s):  
Roksana Khanam ◽  
Ripa Moni ◽  
Md Zahidul Islam ◽  
Md Morsaline Billah ◽  
Umme Salma Zohora ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Bacterial Species ◽  
Toxic Metal ◽  
Extracellular Enzyme ◽  
Bacterial Isolates ◽  
Toxic Heavy Metals ◽  
16S Rrna Sequence ◽  
Yeast Extract Mannitol ◽  
16S Rrna Sequence Analysis

Arsenic is a toxic metal found as inorganic oxyanion arsenate As(V) and arsenite As (III) species. The disposal of toxic heavy metals such as arsenic poses high risk to environment. The present study was undertaken to isolate arsenic-metabolizing bacteria from arsenic contaminated soil of Chandpur district, which is one of the most arsenic contaminated area in Bangladesh and later these bacteria were screened for their ability to metabolize arsenate. Out of ninety eight isolates, ten were found to be capable of metabolizing arsenic in Yeast Extract Mannitol (YEM) medium containing 2 mM arsenate at 37ºC. One of the bacterial isolates designated as I-25 was found to produce an extracellular enzyme which can reduce As(V) into As(III) and able to grow in presence of up to 500 mM arsenate. Subsequent molecular identification of this enzyme producing bacterial isolate using 16s rRNA sequence analysis was correlated with previously identified isolate as Bacillus aryabhatti. Further characterization of the enzyme showed that optimum pH of the extracellular enzyme by the bacterial species was 7 and optimum temperature for the enzyme activity was 60ºC. The bacterial isolates can be exploited for the study of possible bioremediation of arsenic contamination. Jahangirnagar University J. Biol. Sci. 8(1): 57-65, 2019 (June)

Phyto-fabrication of Iron Nanoparticles: Characterization and Antibacterial Capacity

2020 ◽  
Vol 16 ◽  
Author(s):  
Asma S. Algebaly ◽  
Afrah E. Mohammed ◽  
Mudawi M. Elobeid
Keyword(s):  
Electron Microscopy ◽  
Plant Extracts ◽  
Large Scale ◽  
Iron Nanoparticles ◽  
Ethanolic Extract ◽  
Green Technology ◽  
Resistant Bacteria ◽  
Scale Production ◽  
Bacterial Strains ◽  
Plant Sources

Introduction: Fabrication of iron nanoparticles (FeNPs) has recently gained a great concern for their varied applications in remediation technologies of the environment. Objective: The current study aimed to fabricate iron nanoparticles by green technology approach using different plant sources, Azadirachta indica leaf and Calligonum comosum root following two extraction methods. Methods: Currently, a mixture of FeCl2 and FeCl3 was used to react with the plant extracts which are considered as reducing and stabilizing agents for the generation of FeNPs in one step. Different techniques were used for FeNPs identification. Results: Immediately after mixing of the two reaction components, the color changed to dark brown as an indication of safe conversion of Fe ions to FeNPs, that later confirmed by zeta sizer, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). FeNPs fabricated by C. comosum showed smaller size when compared by those fabricated by A. indica. Using both plant sources, FeNPs fabricated by the aqueous extract had smaller size in relation to those fabricated by ethanolic extract. Furthermore, antibacterial ability against two bacterial strains was approved. Conclusion: The current results indicated that, at room temperature plant extracts fabricated Fe ion to Fe nanoparticles, suggesting its probable usage for large scale production as well as its suitability against bacteria. It could also be recommended for antibiotic resistant bacteria.

Adsorptive Removal of Copper from Waste Water Using Biomass & Biochar Based Materials

2022 ◽  
Vol 1048 ◽  
pp. 459-467
Author(s):  
Sadamanti Sireesha ◽  
Utkarsh Upadhyay ◽  
Inkollu Sreedhar ◽  
K.L. Anitha
Keyword(s):  
Adsorption Capacity ◽  
Wheat Bran ◽  
Heavy Metal Contamination ◽  
Copper Ions ◽  
Low Cost ◽  
Toxic Metal ◽  
Environmental Concerns ◽  
Corn Cob ◽  
Mango Kernel ◽  
Neem Leaves

Heavy metal contamination has been one of the primary environmental concerns for many years in most developing countries. As the industries continue to search for low-cost and efficient adsorbents to treat their effluents contaminated with these toxic metal ions, biomass-based adsorbents have gained much attention. This work exploits such ten different biomass-based adsorbents (namely, Karanja de-oiled cake, Neem de-oiled cake, Neem leaves, Moringa Leaves, Bagasse, Mango Kernel, Wheat Bran, Eucalyptus, Fly ash, and Corn cob) for adsorption of copper ions in particular. Further, selected adsorbents (namely Karanja de-oiled cake, Neem de-oiled cake, Bagasse, Wheat Bran and Mango Kernel) were taken to the next stage and modified to biochar and tested again for copper removal. Among the biomass-based adsorbents, the highest adsorption capacity was observed for Neem de-oiled cake (equal to 9.6 mg/g). While for biochar-based adsorbents, Bagasse showed the highest adsorption capacity for copper (equivalent to 13.0 mg/g).

Community of Hydrocarbon-Oxidizing Bacteria in Petroleum Products on the example of Ts-1 Aviation Fuel and AI-95 Gasoline

2021 ◽  
Vol 37 (1) ◽  
pp. 54-68
Author(s):  
E.S. Lobakova ◽  
G.A. Dolnikova ◽  
E.A. Ivanova ◽  
D.A. Sanjieva ◽  
A.A. Burova ◽  
...  
Keyword(s):  
Rhodococcus Erythropolis ◽  
Basic Research ◽  
Petroleum Products ◽  
The State ◽  
Aviation Fuel ◽  
Bacterial Strains ◽  
16S Rrna Sequence ◽  
State Assignment ◽  
Microbial Biofilms ◽  
Microbial Potential

It has been shown that the studied petroleum products (kerosene and gasoline) contain microflocules of heterogeneous microbial biofilms, the cells of which are integrated into a polymer matrix containing acidic polysaccharides. Thirteen bacterial strains were microbiologically isolated from petroleum products, and their taxonomy was identified by the 16S rRNA sequence. Kerosene was characterized by a diverse bacterial composition including the following genera: Sphingobacterium, Alcaligenes, Rhodococcus and Deinococcus, while gasoline bacterial community included only two genera: Bacillus and Paenibacillus. Representatives of the Deinococcus genera capable of growing on the hydrocarbons were isolated from fuels for the first time. The strains isolated from gasoline (Bacillus safensis Bi13 and Bacillus sp. Bi14) proved to be the most effective biodegraders of all n-alkanes, isoalkanes, cycloalkanes, alkenes and aromatic hydrocarbons, whereas the kerosene strain Rhodococcus erythropolis Bi6 effectively decomposed n-alkanes and trimethylbenzene. Both types of petroleum products contained hydrocarbon-oxidizing communities, some members of which were more active in the biodegradation of hydrocarbons, while others were capable of producing biosurfactants and had either emulsifying activity (Deinococcus sp. Bi7) or cell wall hydrophobicity (Sphingobacterium sp. Bi5 from kerosene; Bacillus pumilus Bi12 from gasoline) significantly higher than the average level. The indicated properties of the studied strains make them promising for use in bioremediation. biodegradation, petroleum products, hydrocarbon-oxidizing bacteria, bio-surfactants The work was carried out within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation (topic no. 10.5422.2017/8.9.). Investigation of microbial potential in the use hydrocarbons was supported by the Russian Foundation for Basic Research (RFBR), contract no. 18-29-05067. Physicochemical research was performed within the framework of the state assignment to the TIPS RAS

Nghiên cứu sự kháng kháng sinh của các chủng vi khuẩn gây nhiễm khuẩn đường tiết niệu phân lập được tại Bệnh viện Hữu nghị Đa khoa Nghệ An

2021 ◽  
Author(s):  
Trâm Quế Anh
Keyword(s):  
Urinary Tract ◽  
General Hospital ◽  
Urinary Tract Infections ◽  
Resistant Bacteria ◽  
Antibiotics Resistance ◽  
Bacterial Strains ◽  
E Coli ◽  
Linezolid Resistance ◽  
Drug Resistant Bacteria ◽  
Tract Infections

TÓM TẮT Đặt vấn đề: Xác định đúng căn nguyên gây NKĐTN và mức độ kháng kháng sinh của các vi khuẩn sẽ giúp cho việc điều trị có hiệu quả, giảm được chi phí điều trị, hạn chế sự gia tăng vi khuẩn đề kháng kháng sinh. Đối tượng và phương pháp nghiên cứu: Các chủng VK gây nhiễm khuẩn đương tiết niệu phân lập được tại bệnh viện Hữu nghị Đa khoa Nghệ An từ 1/2020 đến 12/2020. Thiết kế nghiên cứu: Cắt ngang mô tả. Kết quả: Phân lập được 473 chủng vi khuẩn gây NKĐTN, trong đó, E. coli 38,48%; P. aeruginosa 14,15; Enterococcus sp 10,57; K. pneumoniae 13,32%. E. coli: kháng các kháng sinh Cephalosporine, Quinolones từ 56,7 - 63,8%, Carbapenem 4,5 - 6,2%, sinh ESBL 49,4%. P. aeruginosa: đã kháng các kháng sinh thử nghiệm từ 59,1 - 69,2%. Enterococcus sp: kháng với các kháng sinh nhóm Quinolone 73,5%, kháng Vancomycin 8,3%; Chưa ghi nhận đề kháng Linezolid. K. pneumoniae: kháng nhóm Cephalosporin, Quinolone từ 66,7 - 74,6%, đề kháng với Carbapenem từ 46,0 - 50,8%. Kết luận: Các vi khuẩn gây nhiễm khuẩn tiết niệu thường gặp là: E. coli, P. aeruginosa, Enterococcus sp. K. pneumoniae. Các vi khuẩn phân lập được đã đề kháng với nhiều kháng sinh thường dùng với các mức độ khác nhau. Xuất hiện các chủng vi khuẩn Gram âm kháng Carbapenem, Gram dương kháng Vancomycin. Từ khóa: Nhiễm khuẩn tiết niệu, E.coli, Klebsiella, P.aeruginosa, Enterococcus sp ABSTRACT RESEARCH OF ANTIBIOTICS RESISTANCE OF BACTERIA STRAINS CAUSING URINARY TRACT INFECTIONS ISOLATED AT NGHEAN FRIENDSHIP GENERAL HOSPITAL Background: The good identification of UTI microorganism and their antimicrobial susceptibility would promote the effective treatment, reduce the cost as well as the emergence of drug resistant bacteria. Methods: Bacterial strains causing urinary tract infections were isolated at Nghe An Friendship General Hospital from 1/2020 to 12/2020. Study design: Descriptive cross section. Results: 473 bacterial strains causing UTIs were isolated, in which, E. coli 38.48%; P. aeruginosa 14.15; Enterococcus sp 10.57; K. pneumoniae 13.32%. E. coli: resistant to Cephalosporin antibiotics, Quinolones from 56.7 - 63.8%, Carbapenem 4.5 - 6.2%, producing ESBL 49.4%. P. aeruginosa: was resistant to the tested antibiotics from 59.1 - 69.2%. Enterococcus sp: resistant to Quinolone antibiotics 73.5%, resistant to Vancomycin 8.3%; Linezolid resistance has not been recorded. K. pneumoniae: resistant to Cephalosporin, Quinolone from 66.7 - 74.6%, resistant to Carbapenem from 46.0 - 50.8%. Conclusion: Common bacteria causing urinary tract infections are: E. coli, P. aeruginosa, Enterococcus sp. K. pneumoniae. The isolates were resistant to many commonly used antibiotics to varying degrees. Occurrence of strains of Gram - negative bacteria resistant to Carbapenem, Gram - positive resistant to Vancomycin. Keywords: Urinary Tract infections, E. coli, Klebsiella, P. aeruginosa, Enterococcus sp.

scholarly journals Advances in Antarctic Research for Antimicrobial Discovery: A Comprehensive Narrative Review of Bacteria from Antarctic Environments as Potential Sources of Novel Antibiotic Compounds Against Human Pathogens and Microorganisms of Industrial Importance

Antibiotics ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 90 ◽  
Author(s):  
Kattia Núñez-Montero ◽  
Leticia Barrientos
Keyword(s):  
Public Health Problem ◽  
Narrative Review ◽  
Bioactive Molecules ◽  
Resistant Bacteria ◽  
Natural Environments ◽  
Antimicrobial Compounds ◽  
Human Pathogens ◽  
Bacterial Strains ◽  
Antarctic Bacteria ◽  
Recent Emergence

The recent emergence of antibiotic-resistant bacteria has become a critical public health problem. It is also a concern for industries, since multidrug-resistant microorganisms affect the production of many agricultural and food products of economic importance. Therefore, discovering new antibiotics is crucial for controlling pathogens in both clinical and industrial spheres. Most antibiotics have resulted from bioprospecting in natural environments. Today, however, the chances of making novel discoveries of bioactive molecules from various well-known sources have dramatically diminished. Consequently, unexplored and unique environments have become more likely avenues for discovering novel antimicrobial metabolites from bacteria. Due to their extreme polar environment, Antarctic bacteria in particular have been reported as a potential source for new antimicrobial compounds. We conducted a narrative review of the literature about findings relating to the production of antimicrobial compounds by Antarctic bacteria, showing how bacterial adaptation to extreme Antarctic conditions confers the ability to produce these compounds. We highlighted the diversity of antibiotic-producing Antarctic microorganisms, including the phyla Proteobacteria, Actinobacteria, Cyanobacteria, Firmicutes, and Bacteroidetes, which has led to the identification of new antibiotic molecules and supports the belief that research on Antarctic bacterial strains has important potential for biotechnology applications, while providing a better understanding of polar ecosystems.

scholarly journals Heavy metal composition of maize and tomato grown on contaminated soils

2018 ◽  
Vol 3 (1) ◽  
pp. 414-426
Author(s):  
A.O. Adekiya ◽  
A.P. Oloruntoba ◽  
S.O. Ojeniyi ◽  
B.S. Ewulo
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Contaminated Soils ◽  
Heavy Metal Contamination ◽  
Mining Area ◽  
Atomic Absorption Spectrophotometry ◽  
Toxic Heavy Metals ◽  
Mining Site ◽  
Solanum Lycopersicum L ◽  
Sources Of Pollution

Abstract The study investigated the level of heavy metal contamination in plants {maize (Zea mays) and tomato (Solanum lycopersicum L.)} from thirty soil samples of three locations (Epe, Igun and Ijana) in the Ilesha gold mining area, Osun State, Nigeria. Total concentrations of As, Cd, Co, Cr, Cu, Ni, Pb and Zn were determined using atomic absorption spectrophotometry. Spatial variations were observed for all metals across the locations which was adduced to pH and the clay contents of the soils of each location. The results showed that heavy metals are more concentrated in the areas that are closer to the mining site and the concentrations in soil and plants (maize and tomato) decreased with increasing perpendicular distance from the mining site, indicating that the gold mine was the main sources of pollution. The mean concentrations of heavy metals in plants (tomato and maize) samples were considered to be contaminated as As, Cd and Pb respectively ranged from 0.6 - 2.04 mg kg-1, 0.8 - 5.2 mg kg-1, 0.8 - 3.04 mg kg-1 for tomato and respectively 0.60 - 2.00 mg kg-1, 1.50 - 4.60 mg kg-1 and 0.90 - 2.50 mg kg-1 for maize. These levels exceeded the maximum permissible limits set by FAO/WHO for vegetables. In conclusion, monitoring of crops for toxic heavy metals is essential for food safety in Nigeria.

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