scholarly journals Amazonian soil fungi are efficient degraders of glyphosate herbicide; novel isolates of Penicillium, Aspergillus, and Trichoderma

2023 ◽  
Vol 83 ◽  
Author(s):  
L. O. Correa ◽  
A. F. M. Bezerra ◽  
L. R. S. Honorato ◽  
A. C. A. Cortez ◽  
J. V. B. Souza ◽  
...  
Keyword(s):  
Soil Fungi ◽  
Hydrological Cycle ◽  
Culture Media ◽  
Carbon Substrate ◽  
Metabolic Versatility ◽  
Aminomethylphosphonic Acid ◽  
Amazonian Rainforest ◽  
Glyphosate Herbicide ◽  
Glyphosate Degradation ◽  
Herbicide Glyphosate

Abstract Pesticide residues that contaminate the environment circulate within the hydrological cycle can accumulate within the food chain and cause problems to both environmental and human health. Microbes, however, are well known for their metabolic versatility and the ability to degrade chemically stable substances, including recalcitrant xenobiotics. The current study focused on bio-prospecting within Amazonian rainforest soils to find novel strains fungi capable of efficiently degrading the agriculturally and environmentally ubiquitous herbicide, glyphosate. Of 50 fungal strains isolated (using culture media supplemented with glyphosate as the sole carbon-substrate), the majority were Penicillium strains (60%) and the others were Aspergillus and Trichoderma strains (26 and 8%, respectively). All 50 fungal isolates could use glyphosate as a phosphorous source. Eight of these isolates grew better on glyphosate-supplemented media than on regular Czapek Dox medium. LC-MS revealed that glyphosate degradation by Penicillium 4A21 resulted in sarcosine and aminomethylphosphonic acid.

scholarly journals Effects of Glyphosate-Based and Derived Products on Sea Urchin Larval Development

2020 ◽  
Vol 8 (9) ◽  
pp. 661
Author(s):  
Davide Asnicar ◽  
Costanza Cappelli ◽  
Ahmad Safuan Sallehuddin ◽  
Nur Atiqah Maznan ◽  
Maria Gabriella Marin
Keyword(s):  
Larval Development ◽  
Sea Urchin ◽  
Marine Invertebrates ◽  
Parent Compound ◽  
Paracentrotus Lividus ◽  
Marine Species ◽  
Negative Effects ◽  
Aminomethylphosphonic Acid ◽  
Main Degradation Product ◽  
Herbicide Glyphosate

Despite the widespread use of herbicide glyphosate in cultivation, its extensive runoff into rivers and to coastal areas, and the persistence of this chemical and its main degradation product (aminomethylphosphonic acid, AMPA) in the environment, there is still little information on the potential negative effects of glyphosate, its commercial formulation Roundup® and AMPA on marine species. This study was conducted with the aim of providing a comparative evaluation of the effects of glyphosate-based and its derived chemicals on the larval development of the sea urchin Paracentrotus lividus, thus providing new data to describe the potential ecotoxicity of these contaminants. In particular, the effects on larval development, growth and metabolism were assessed during 48 h of exposure from the time of egg fertilization. The results confirm that AMPA and its parent compound, glyphosate have similar toxicity, as observed in other marine invertebrates. However, interestingly, the Roundup® formulation seemed to be less toxic than the glyphosate alone.

The fate of a hazardous herbicide: a DFT-based ab initio study on glyphosate degradation

2021 ◽  
Author(s):  
Malek Sadatsharifi ◽  
Daniel W. Ingersoll ◽  
Mihály Purgel
Keyword(s):  
Ab Initio ◽  
Peroxyl Radicals ◽  
Ab Initio Study ◽  
Glyphosate Degradation ◽  
Herbicide Glyphosate

Interaction of the well-known herbicide, glyphosate, with small radicals like hydroxyl and peroxyl radicals, such that the reaction between glyphosate radicals and oxygen molecules results in different species.

scholarly journals Development of RR soybean in function of glyphosate doses and Bradyrhizobium inoculation

2018 ◽  
Vol 22 (12) ◽  
pp. 854-858
Author(s):  
João W. Bossolani ◽  
Nadia M. Poloni ◽  
Edson Lazarini ◽  
João V. T. Bettiol ◽  
João A. Fischer Filho ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Symbiotic Nitrogen Fixation ◽  
Soybean Seeds ◽  
Leaf Chlorophyll ◽  
Outdoor Experiment ◽  
Glyphosate Herbicide ◽  
Soybean Plants ◽  
Biological Nitrogen ◽  
Different Doses ◽  
Herbicide Glyphosate

ABSTRACT Soybean has traditionally been produced in systems that include the use of herbicides, often in higher than recommended doses. The process of symbiotic nitrogen fixation in legumes can be hampered by these herbicides, both by direct effects on rhizobia and indirect effects on the host plant. An outdoor experiment was performed to evaluate the effects of different doses of a glyphosate herbicide on Bradyrhizobium strains and biological nitrogen fixation in soybean BMX Potência RR plants. Soybean seeds were inoculated with Bradyrhizobium elkanii (SEMIA 5019) and Bradyrhizobium japonicum (SEMIA 5079) strains in a commercial liquid inoculant. The treatments consisted of the absence and presence of Bradyrhizobium genotypes inoculated via seed and four doses of the herbicide glyphosate applied on the leaves (0, 1.0, 2.0, and 4.0 L ha-1 of the commercial product) at the V3 stage. The leaf chlorophyll index of inoculated RR soybean plants did not change on the application of glyphosate and, regardless of inoculation, plants had the capacity to recover from the effects of glyphosate application, without impaired development.

scholarly journals DETERMINAÇÃO DE GLIFOSATO E ÁCIDO AMINOMETILFOSFÔNICO EM ÁGUAS SUPERFICIAIS DO ARROIO PASSO DO PILÃO

2003 ◽  
Vol 13 ◽  
Author(s):  
MARCELO DUTRA DA SILVA ◽  
MARIA DO CARMO RUARO PERALBA ◽  
MARIA LAURA TURINO MATTOS
Keyword(s):  
High Performance Liquid Chromatography ◽  
Environmental Protection Agency ◽  
High Performance ◽  
Herbicide Application ◽  
Protection Agency ◽  
Brazilian Legislation ◽  
Aminomethylphosphonic Acid ◽  
Glyphosate Herbicide ◽  
High Concentrations

Para investigar a presença do herbicida Glifosato na microbacia hidrográfica arroio Passo do Pilão foram coletadas amostras de água em 15 distintos pontos no arroio Passo do Pilão, nos períodos de 30 e 60 dias após a aplicação do herbicida (DAAH), as quais foram analisadas por cromatografia a líquido de alta eficiência (CLAE). As análises revelaram a presença do herbicida nas águas superficiais dessa microbacia, tanto nas amostras após 30 dias de aplicação do Glifosato como nas de 60 DAAH. Concentrações elevadas (acima de 100 ppb) foram detectadas, principalmente em pontos próximos às áreas de intenso cultivo. As concentrações detectadas foram menores que 500 e 700 ppb, limites de concentrações máximas permitidas para o Glifosato pela legislação brasileira e pela Agência de Proteção Ambiental dos Estados Unidos, respectivamente. DETERMINATION OF GLYPHOSATE AND AMINOMETHYLPHOSPHONIC ACID IN SUPERFICIAL WATERS OF ARROIO PASSO DO PILÃO Abstract To investigation the presence of Glyphosate herbicide in Arroio Passo do Pilão watershed, samples of water were collected in 15 distinct points in Arroio Passo do Pilão, in period of 30 and 60 days after herbicide application (DAHA), which were analysed by high performance liquid chromatography (HPLC). The analysis revealed the presence of the herbicide in superficial areas of this watershed, even in the samples after 30 days of Glyphosate applicatication as in 60 DAHA. High concentrations (above 100 ppb) were detected, mainly in points near to intense cultivation areas. The concentrations detected were smaller than 500 and 700 ppb, limits of the maximum concentrations allowed for Glyphosate by the brazilian legislation and by the Environmental Protection Agency of United States, respectivelly.

Some Aspects of the Influence of Residual Amounts of Glyphosate Herbicide in Soil On Cultural Plants

2020 ◽  
Vol 24 (5) ◽  
pp. 24-27
Author(s):  
I.A. Dobrosmyslova ◽  
A.A. Sazanova ◽  
O.E. Nasakin
Keyword(s):  
Immune System ◽  
Control Sample ◽  
Sharp Decrease ◽  
Beta Carotene ◽  
Vitamin B2 ◽  
Untreated Soil ◽  
Pea Seedlings ◽  
Glyphosate Herbicide ◽  
The Difference ◽  
Herbicide Glyphosate

Studies have been conducted on the effect of residual quantities of the most common herbicide Glyphosate (0.8; 1.4; 17) mg / kg of soil on the greens of peas and oats. Spectrophotometrically analyzed samples for their content of beta-carotene, which protects the cells of the immune system from damage by free radicals and can improve the immune system. The data obtained showed that the higher the content of herbicide in the soil, the less beta-carotene in samples of hay. The results of the experiments showed that the height of pea seedlings decreases when the soil is treated with Glyphosate. When the concentration of the herbicide is 1.4 mg / kg, it decreases by 32 % compared to the control; when selenium is added, the difference compared to untreated soil is 23 %. less than in the control sample. A sharp decrease in vitamin B2 and vitamin A in greens of peas grown in soil treated with Glyphosate is shown. The content of vitamins increases with the addition of selenium to the soil. The obtained experimental data indicate a decrease in beta-carotene, vitamins A and B2 in greens grown on soil containing Glyphosate.

scholarly journals Alternative methods for detecting soybean seeds genetically modified for resistance to herbicide glyphosate

2013 ◽  
Vol 35 (3) ◽  
pp. 381-386
Author(s):  
Lilian Faria de Melo ◽  
Marcelo Fagioli ◽  
Marco Eustáquio de Sá
Keyword(s):  
Genetically Modified ◽  
Alternative Methods ◽  
Soybean Seeds ◽  
Transgenic Soybean ◽  
Genetically Modified Soybean ◽  
Soybean Cultivars ◽  
Seed Analysis ◽  
Glyphosate Herbicide ◽  
Herbicide Glyphosate

The objective of this study was to verify application of two methodologies: substrate moistened with herbicide solution (SM) and immersion of seeds in herbicide solution (IH) for detecting soybean seeds genetically modified. For this, non-transgenic and transgenic soybean seeds, harvested in the 2008/2009 crop seasons were used. The treatments with substrate moistened were: SM1) 0.03% herbicide solution, at 25 ºC, with evaluation in the sixth day (hs -0.03% -25 ºC, 6th d); SM2) HS -0.03% -35 ºC, 5th d; SM3) HS -0.03% - 40 ºC, 5th d; and SM4) hs -0.06% -5 ºC, 5th d. In the methodology of immersion of seeds the following treatments were performed: IH1) seed immersion in a 0.6% herbicide solution, at 25 ºC, for 1 h, (si -0.06% -25 ºC, 1 h; IH2) si -0.06% - 35 ºC, 30 min.; IH3) si -0.06% -40 ºC, 30 min.; IH4) si -0.12% -35 ºC, 30 min.; and IH5) si -0.12% -40 ºC, 30 min. Bioassays allow detecting soybean seeds tolerant to glyphosate herbicide within five days. The seeds of non-genetically modified and genetically modified soybean cultivars may be easily distinguished through the treatments SM2 and SM4 of the moistened substrate methodology; and treatments IH3, IH4, and IH5 of seed immersion methodology. Both methodologies are easily feasible, practical, and applicable in seed analysis laboratories, once do not require special equipments.

Resistance to degradation and effect of the herbicide glyphosate on the bacterioplankton community of a large river system dominated by agricultural activities

2020 ◽  
Vol 71 (8) ◽  
pp. 1026
Author(s):  
Claudia Piccini ◽  
Stefano Fazi ◽  
Germán Pérez ◽  
Giampiero Batani ◽  
Gabriela Martínez de la Escalera ◽  
...  
Keyword(s):  
Intergenic Spacer ◽  
Bacterial Biomass ◽  
River System ◽  
Degradation Pathway ◽  
Large River ◽  
Bacterioplankton Community ◽  
Aminomethylphosphonic Acid ◽  
Significant Enrichment ◽  
Glyphosate Degradation

Glyphosate-based herbicides are widely used for several crops, such as transgenic soybean and forestry. The aim of this study was to determine the effect of glyphosate on the community structure of riverine bacterioplankton and to evaluate the potential of bacterioplankton to degrade the herbicide. River water to which 13C-labelled glyphosate (10, 100µgL–1) was added or not (control) was incubated for 6 days at the temperature measured in situ (20°C). Significant differences in bacterioplankton community composition, as assessed by microfluidics-based automated ribosomal intergenic spacer analysis, were found among treatments, with differences in the presence of 100µgL–1 of glyphosate being more pronounced, namely significant decreases in bacterial richness and diversity. The glyphosate degradation product aminomethylphosphonic acid (AMPA) was detected, accounting for 1.2% of glyphosate conversion in water with 100µgL–1 of 13C-labelled glyphosate, together with a significant enrichment of 13C in the bacterial biomass. These findings suggest that glyphosate had a direct detrimental effect on most bacterioplankton taxa, but enriched those that were able to degrade the herbicide. Together, the results indicate that glyphosate degradation in the river assessed would be a slow process (months–years), taking place through the AMPA degradation pathway and meaning glyphosate accumulate in the ecosystem.

scholarly journals MONITORAMENTO AMBIENTAL DO GLIFOSATO E DO SEU METABÓLITO (ÁCIDO AMINOMETILFOSFÔNICO) NA ÁGUA DE LAVOURA DE ARROZ IRRIGADO

2002 ◽  
Vol 12 ◽  
Author(s):  
MARIA LAURA TURINO MATTOS ◽  
MARIA DO CARMO RUARO PERALBA ◽  
SILVIO L. P. DIAS ◽  
FÁBIO PRATA ◽  
LARISSA CAMARGO
Keyword(s):  
United States ◽  
Environmental Protection ◽  
Environmental Protection Agency ◽  
The United States ◽  
Concentration Limit ◽  
Protection Agency ◽  
Irrigation Channel ◽  
Channel Water ◽  
Aminomethylphosphonic Acid ◽  
Herbicide Glyphosate

Monitorou-se a presença do herbicida Glifosato e do seu metabólito, ácido aminometilfosfônico (AMPA), em amostras de águas coletadas em área orizícola, submetida a plantio direto. Níveis de Glifosato em concentrações acima do limite máximo permitido (7,0 µg/L) pela Agência de Proteção Ambiental dos Estados Unidos (Environmental Protection Agency) foram detectados. A presença do metabólito AMPA, nas águas dos canais de irrigação, foi detectada até 120 dias após a aplicação (DAA). Pode-se dizer que determinado nível de segurança será alcançado nas águas de lançamento da granja para o Arroio Bretanhas, 120 DAA da formulação de Glifosato. Como não foi observada deserção do Glifosato em GLEYSSOLO HÁPLICO Ta Eutrófico pode-se inferir que a concentração do mesmo permaneceu como resíduo ligado. Assim, a dissipação do herbicida nesse solo pode ser reflexo, entre outros fatores, da formação de resíduo ligado, determinando desta forma o seu destino e/ou comportamento no ambiente. ENVIRONMENTAL MONITORING OF GLYPHOSATE AND ITS METABOLITE (AMINOMETHYLPHOSPHONIC ACID) IN TILLAGE WATER OF IRRIGABLE RICE Abstract Monitor the presence of the herbicide glyphosate and its metabolite, aminomethylphosphonic acid (AMPA), in samples of water collected in orizicole area, subject to direct plantation, was the objective of this study. Levels of glyphosate above the maximum permitted concentration limit (7.0 µg/L) were detected by the United States Environmental Protection Agency (EPA). The presence of the metabolite AMPA in irrigation channel water was detected up to 120 days after application (DAA). It can be deduced that a certain level of safety will be reached 120 DAA from formulation of glyphosate, for water from the Arroio Bretanhas farm. Since the desertion of glyphosate in a Gleyssolo Háplico Ta Eutrophic was not observed, it can be judged that the concentration of it remained as bonded residue. Thus, the dissipation of this herbicide in this soil can be a reflex of, among other factors, the formation of bonded residue, determining, in this manner, its destiny and/or behavior in the environment.

Optimization and Validation of a Residue Analysis Method for Glyphosate, Glufosinate, and Their Metabolites in Plant Matrixes by Liquid Chromatography with Tandem Mass Spectrometry

2017 ◽  
Vol 100 (3) ◽  
pp. 631-639 ◽  
Author(s):  
Dasharath P Oulkar ◽  
Sandip Hingmire ◽  
Arnab Goon ◽  
Manjusha Jadhav ◽  
Bharat Ugare ◽  
...  
Keyword(s):  
Residue Analysis ◽  
Direct Analysis ◽  
Field Trials ◽  
Cotton Leaf ◽  
Solvent Ratio ◽  
Extraction Solvent ◽  
Aminomethylphosphonic Acid ◽  
Cleanup Procedure ◽  
Aqueous Layer ◽  
Herbicide Glyphosate

Abstract A sensitive and accurate LC with tandem MS (MS/MS)-based method was developed and validated for the analysis of the herbicide glyphosate, its metabolite aminomethylphosphonic acid (AMPA), and glufosinate after derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) in various plant matrixes. The method also covers direct analysis of the glufosinate metabolites 3-methylphosphinicopropionic acid (3-MPPA) and N-acetyl-glufosinate (NAG). The homogenized samples were extracted with 0.1% formic acid in water–dichloromethane (50 + 50). The aqueous layer was derivatized with FMOC-Cl, cleaned through an HLB SPE cartridge, and determined by LC-MS/MS. The sample size, extraction solvent, sample-to-solvent ratio, derivatization conditions,and cleanup procedure were thoroughly optimized, the LOQs of glyphosate, glufosinate, and AMPA were 0.5 ng/g in grape, corn (leaf and seed), and cotton (leaf, seed, and oil) and 2 ng/g in soybean and tea. The LOQs of NAGand 3-MPPA were 50 ng/g in all the test matrixes, except tea and soybean, for which the LOQ was 100 ng/g. Inall cases, average recoveries were >80%. The method successfully performed the estimation of glyphosate in incurred corn and cotton leaf samples collected from supervised field trials.

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