scholarly journals Evaluation of methanolic crude extract of Linum usitatissimum for the removal of biofilm in diabetic foot isolates

2023 ◽  
Vol 83 ◽  
Author(s):  
M. Haroon ◽  
M. J. Iqbal ◽  
W. Hassan ◽  
S. Ali ◽  
H. Ahmed ◽  
...  
Keyword(s):  
Crude Extract ◽  
Diabetic Foot ◽  
Linum Usitatissimum ◽  
Study Groups ◽  
Proximate Analysis ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Gram Positive Bacteria ◽  
Linum Usitatissimum L ◽  
Proximate Analyses

Abstract Linum usitatissimum L is a widely used traditionally for multiple ailments. The present research was carried out to explore the antimicrobial, and anti-biofilm activity of crude extract of Linum usitatissimum L (Lu. Cr). Phytochemical and proximate analyses were performed. The bandages of diabetic foot patients were collected from the various hospitals. The bandages were cultured to isolate the bacterial strains present on it. The disc diffusion method was used to identify the antimicrobial potential whereas the minimum inhibitory concentration of the Lu.Cr were also determined. Proximate analysis confirms moisture content 8.33%, ash content 4.33%, crude protein 21.20%, crude fat 49.2% and crude fiber 5.63%. It was revealed that Gram-positive bacteria are most prevalent among all study groups. Lu.Cr possess significant bactericidal potential against S. aureus among all other microbes. Owing to this potential, linseed coated bandages can be used alternatively for the treatment of diabetic foot.

scholarly journals Evaluation of Anti-Microbial Activity and Proximate Composition of Alkaloids from Vitex doniana Seed

2021 ◽  
Vol 20 (1) ◽  
pp. 81-86
Author(s):  
Theophilus K Udeani ◽  
Linus O Ugwu ◽  
Charles O Nnadi ◽  
Chukwugozie N Okwuosa
Keyword(s):  
Microbial Activity ◽  
Crude Extract ◽  
Salmonella Typhi ◽  
Proximate Analysis ◽  
Diffusion Method ◽  
Significant Activity ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Vitex Doniana

The study investigated the in vitro anti-microbial activity of crude alkaloids from the seeds of Vitex doniana against some selected bacteria and fungal strains. Agar well diffusion method was used to test the antimicrobial activity using ciprofloxacin and ketoconazole as controls. Phytochemical and proximate analyses were carried out by standard methods. The crude extract showed the presence of alkaloids, tannins and resins in abundance. Proximate analysis indicated fat/oil (11±0.73 %), protein (0.26±0.02 %), moisture (0.21±0.01 %), ash (5.0±0.22 %) and carbohydrate (6.42±0.28 %). The antimicrobial study indicated that the crude extract was more effective against Pseudomonas aeruginosa (MIC 3.84 mg/ml) and Salmonella typhi (MIC 3.84 mg/ml) and other Gram-positive bacteria. The crude alkaloids generally showed lower activity in case of Gram-negative (MIC Salmonella typhi 4.20 mg/ml) than in Gram-positive bacteria (MIC Staphylococcus aureus 2.52 mg/ml). Surprisingly, the crude alkaloids from the seeds, in addition to improved activity against all the bacteria strains, showed significant activity against Candida albicans (MIC 1.18 mg/mL. V. doniana seed extract was found to be potent against some clinical strains of both Gram-positive and negative bacteria but not fungi; however, its alkaloids has promising antifungal activity. Dhaka Univ. J. Pharm. Sci. 20(1): 81-86, 2021 (June)

scholarly journals EVALUATING THE ANTI-BACTERIAL ACTIVITY OF MYCELIUM EXTRACT FROM DIFFERENT WOOD-DECAY-FUNGUS SPECIES COLLECTED IN SOUTHERN VIETNAM

2018 ◽  
Vol 15 (4) ◽  
pp. 711-720
Author(s):  
Pham Thi Hoa ◽  
Ngo Nguyen Vu ◽  
Tran Thi Phuong Linh
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Wood Decay ◽  
Diffusion Method ◽  
Screening Methods ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Pycnoporus Sanguineus ◽  
Southern Vietnam ◽  
Fungal Isolates

The antibacterial activity of twelve different wood-decay fungal isolated in Southern Vietnam was investigated to find out the new potential antibiotics produced by macro fungi. The antibacterial activity of the collected fungal isolates was tested by three screening methods against seven different strains of bacteria: Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Vibrio parahaemolyticus, Vibrio cholerae, and Salmonella typhi. The screening test were performed at first with mycelium grew on surface of agar medium similar to a disc diffusion test, next by an extracellular metabolite test by flood-plate method, and finally with an intracellular metabolite test using grounded mycelium fluid. To determine the activity of metabolites produced in the mycelium, the fungal isolates were grown on culture substrates to promote mycelium formation. Antibacterial activity of the selected fungi was confirmed by disc diffusion method using crude extract of the mycelium harvested from the substrate cultures. Four fungal species identified with strong antibacterial compounds produced by their mycelium including Pycnoporus sanguineus, Flavodon flavus, Fomitopsis ostreiformis and Meripilus giganteus. Crude extract of Pycnoporus sanguineus showed the strongest antibacterial activity, with 7/7 bacterial strains inhibited. Fomitopsis ostreiformis antibacterial activity was recognized with the ability to inhibit 6/7 bacterial strains. Flavodon flavus and Meripilus giganteus showed weaker activity than other two species, with only 4/7 strains inhibited. The fungal isolates identified with antibacterial activity discovered in this study can be potential candidates for the new source of antibiotic to fight against new generations of antibiotic resistance bacteria in the future.

scholarly journals Extraction and Isolation of Compound from Roots of Kniphofia pumila, and Its Antibacterial Activities in Combination with Zinc Chloride

2020 ◽  
Vol 17 (7) ◽  
pp. 631-638
Author(s):  
Dele ABDISSA ◽  
Gezahegn FAYE ◽  
Melkamu FAYERA ◽  
Shiferaw DEMISSIE
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Spectroscopic Techniques ◽  
Bacterial Strains ◽  
Pure Compound ◽  
Standard Drug ◽  
Isolated Compound

Kniphofia pumila has been traditionally used for the treatment of different diseases in Oromiya regional state, western part of Ethiopia. Thus, the main objective of this study was to extract bioactive natural products from the roots of K. pumila and perform in vitro antibacterial activity tests against selected pathogens. Extraction of compound from the roots of K. pumila was carried out by maceration method at room temperature and its antibacterial activity was done using agar disc diffusion method. The crude extracts alone and along with ZnCl2 were tested against pathogenic bacteria Escherichia coli (ATCC 25722), Klebsiella pneumoniae subsp. pneumoniae (DSM 19613), Staphylococcus aureus (ATCC 25925) and Salmonella Typhimurium (ATCC 13311). Following its promising activity, the acetone crude extract that have shown better antibacterial activity was subjected to column chromatography for isolation of pure compound. Hence, one pure compound (GZ-1) was obtained from acetone crude extract. Meanwhile, the antibacterial activities of the isolated compound alone and in combination with ZnCl2 were also performed against all aforementioned bacterial strains. Then, isolated compound was characterized by using NMR spectroscopic techniques such as 1H-NMR, 13C-NMR and, 2D NMR, so as to establish its structure as 3’-acetyl-2’,6’-dihydroxy-4-methoxyphenyl-1,8-dihydroxy-3-methylanthraquinone, trivial name knipholone. Finally, GZ-1 was isolated from the roots of K. pumila and it was noted that the antibacterial activities of ZnCl2 in combination with crude extract as well as with isolated compound against E. coli (ATCC 25722), S. aureus (ATCC 25925) and S. Typhimurium (ATCC 13311) bacterial strains showed remarkable results which were greater than the antibacterial activity of the positive standard drug (gentamycin). To sum up, from the chromatographic isolation of roots acetone extract of K. pumila one pure compound (knipholone) was obtained. Its antibacterial activity was also evaluated in combination with ZnCl2 salt and alone.

scholarly journals Escherichia coli and Staphylococcus aureus responsible for bovine mastitis sensitivity to the essential oil of Algerian Thymus fontanesii Boiss. et Reut.

2020 ◽  
Vol 7 (1) ◽  
pp. 26-32
Author(s):  
Bendella Amina nor elhouda ◽  
Ghazi Kheira ◽  
Meliani Samia
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Bovine Mastitis ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Zones Of Inhibition

AbstractThe aim of this study is to test two different methods for evaluating the in vitro antibacterial effect of Thymus fontanesii Boiss. et Reut. essential oil against standard and clinical bacterial strains responsible for bovine mastitis: the disc diffusion method or the aromatogram which allows the demonstration of the antibacterial power of essential oils on the bacterial strains tested, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and two strains isolated from bovine mastitis milk S. aureus and E. coli. The inhibition activity of the essential oil of T. fontanesii on bacterial strains by the two methods shows that the antimicrobial power of this oil is very important and is characterized by bactericidal and bacteriostatic action against gram negative and gram positive bacteria. The antimicrobial evaluation by the aromatogram showed good antibacterial activity against all the strains tested, the zones of inhibition of the bacteria were between 23,33±1,527mm and 37,5±3,535 mm. The search for minimum inhibitory concentrations MIC and bactericides CMB made it possible to quantitatively assess the antimicrobial power of this essential oil. In this work, the MIC was 0,625 µl/ml for all strains tested, and the lowest CMB was that of T. fontanesii against E. coli ATCC 25922 was 0,625 µl/ml.

scholarly journals Antibacterial activity of Beta vulgaris L. pomace extract

2011 ◽  
pp. 263-269 ◽  
Author(s):  
Aleksandra Velicanski ◽  
Dragoljub Cvetkovic ◽  
Sinisa Markov ◽  
Jelena Vulic ◽  
Sonja Djilas
Keyword(s):  
Antibacterial Activity ◽  
Beta Vulgaris ◽  
Staphylococcus Epidermidis ◽  
Control Sample ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Natural Isolates

Antibacterial activity of Beta vulgaris L. (beetroot) pomace extract (concentration 100 mg/ml) was tested against five Gram positive and seven Gram negative bacterial strains (reference cultures and natural isolates). Disc diffusion method with 15 ?l of extract and agar-well diffusion method with 50 and 100 ?l were used. Antibiotic (cefotaxime/clavulanic acid) was used as a control sample. The tested extract showed the highest activity against Staphylococcus aureus and Bacillus cereus, where clear zones (without growth) appeared. There was no any activity against other tested Gram-positive bacteria, except for Staphylococcus epidermidis, with a small zone of reduced growth. Growth of all tested Gram-negative bacteria was inhibited usually with 100 ?l of extract. The most susceptible were Citrobacter freundii and Salmonella typhymurium. The tested antibiotic gave clear, usually large zones for all tested strains except for Staphylococcus cohni spp. cohni, where only a zone of reduced growth appeared.

scholarly journals High Efficacy of Ozonated Oils on the Removal of Biofilms Produced by Methicillin-Resistant Staphylococcus aureus (MRSA) from Infected Diabetic Foot Ulcers

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3601
Author(s):  
Vanessa Silva ◽  
Cecília Peirone ◽  
Joana S. Amaral ◽  
Rosa Capita ◽  
Carlos Alonso-Calleja ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Diabetic Foot ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Foot Ulcers ◽  
Diabetic Foot Ulcers ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
Methicillin Resistant ◽  
Mrsa Strains

Ozone has a high wound healing capacity and antibacterial properties and can be used as a complementary treatment in infections. Methicillin-resistant S. aureus (MRSA) is the most common pathogen found in infected diabetic foot ulcers. Most of MRSA are resistant to several classes of antibiotics and, therefore, there is a need for new, effective, and well-tolerated agents. Thus, we aimed evaluate the antimicrobial and antibiofilm potentials of ozonated vegetable oils against MRSA strains isolated from diabetic foot ulcers. Six ozonated oils were produced with concentrations of ozone ranging from 0.53 to 17 mg of ozone/g of oil. The peroxide values were determined for each oil. Ozonated oils content on fatty acid was determined by gas chromatography equipped with a flame ionization detector. The antimicrobial susceptibility testing was performed by the Kirby–Bauer disk diffusion method and the effect of ozonated oils on biofilm formation ability and on established biofilms was investigated. In general, the content in identified unsaturated fatty acid in oils decreased with the increase of ozonation time and, consequently, the peroxide value increased. Most bacterial strains were inhibited by ozonated oil at a concentration of 4.24 mg/g. Ozonated oils had moderate to high ability to remove adhered cells and showed a high capacity to eradicate 24 h old biofilms. Our results show promising use of ozonated oils on the treatment of infections, in particular those caused by multidrug-resistant MRSA strains.

scholarly journals Synthesis, characterization, and in vitro antimicrobial investigation of novel pyran derivatives based on 8-hydroxyquinoline

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Mohamed Rbaa ◽  
Abdelhadi Hichar ◽  
Omar Bazdi ◽  
Younes Lakhrissi ◽  
Khadija Ounine ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Magnetic Resonance ◽  
Penicillin G ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Wide Range

Abstract Background 8-Hydroxyquinoline derivatives are known for their extensive applications in the field of analytical chemistry and separation techniques; their complexes with transition metals also exhibit antibacterial and antifungal activity. Results In the present study, we synthesized a new series of pyranoquinoline derivatives and evaluated their antibacterial activities. The structures of the synthesized compounds were characterized by Fourier transform infrared (FT-IR), hydrogen-1 nuclear magnetic resonance, carbon-13 nuclear magnetic resonance, and elemental analysis. All the prepared compounds were evaluated in vitro as antimicrobial agents against Gram-positive and Gram-negative bacterial strains (Escherichia coli (ATCC35218), Staphylococcus aureus (ATCC29213), Vibrio parahaemolyticus (ATCC17802), and Pseudomonas aeruginosa (ATCC27853)). The screening test was determined by using the standard protocol of disc diffusion method (DDM). Conclusion We have synthesized new pyranic compounds bearing an 8-hydroxyquinoline moiety on their structure. The preliminary screening results showed that all the tested compounds have a remarkable inhibitory effect on the growth of the majority of the tested bacterial strains compared to the standard antibiotic (penicillin G), and the chlorinated compound (Q1) is more active against Gram-positive bacteria than Gram-negative bacteria such as the Staphylococcus aureus strain which is the most sensitive. Gram-positive bacteria are responsible for a wide range of infectious diseases, and rising resistance in this group is causing increasing concern. Thus, this study develops novel heterocyclic compound derivatives of 8-hydroxyquinoline that have demonstrated good antibacterial activity against Gram-positive bacteria. Graphical abstract

scholarly journals Antimicrobial activity of crude henna extract against Gram-positive bacteria

2021 ◽  
Vol 5 (3) ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Crude Extract ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Drug Resistant ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Bacterial Agent ◽  
Henna Extract

Objectives: This study aimed to examine the effect of ethanolic extract of local Basra henna leaves on Gram-positive bacteria species. Also, to assess the antibacterial properties of henna crude extract in vitro and compare them with antibiotics. Methods: In this study, Lawsonia inermis (henna) leaves were extracted with ethanol using the solvent extraction technique. The pathogens were isolated from wound samples obtained from hospitalized patients in two different hospitals in Duhok city. The culture of thirty isolates had been recognized by routine methods. Different concentrations of ethanol crude extract were acquired and bio-assayed in vitro to inhibit the growth of five human pathogenic Gram-positive bacteria. Agar well diffusion assay was used for achieving henna antibiotic activity. Moreover, an antibiotics susceptibility test was done by the disk diffusion method using the Muller-Hinton agar medium. Results: The growth of all tested bacteria was suppressed to various degrees by increasing the concentration of the extract. The data has revealed that Staphylococcus aureus was more sensitive than other examined isolates, where the diameter zone of inhibition was ranging from 16-27, 14-25, and 8-18 mm for Staphylococcus epidermidis, Lactobacillus spp. and Streptococcus pneumonia respectively. The antimicrobial activity of henna extract indicates that it is suitable for being used as significant certain medications. Consequently, henna is active to serve as an anti-bacterial agent against multi-drug resistant Gram-positive bacteria. Conclusion: The antimicrobial activity of henna extract indicates that it is suitable for being used as significant certain medications. Consequently, henna is active to serve as an anti-bacterial agent against multi-drug resistant Gram-positive bacteria.

scholarly journals Antimicrobial Activity of Chloroform and Methanol Extract of Lennea coromandelica Merr

2015 ◽  
Vol 45 ◽  
pp. 67-74 ◽  
Author(s):  
P. Vedhanarayanan ◽  
T. Vaithiyanathan ◽  
P. Sundaramoorthy
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Methanol Extracts ◽  
Gram Positive Bacteria ◽  
Zones Of Inhibition ◽  
Inhibition Zones ◽  
Bacterial Concentrations

The antimicrobial activity of chloroform and methanol extracts of Lennea coromandelica were screened for their was studied against gram positive bacteria strains Staphylococcus aureus,Streptococcus pyogenes and Bacillus subtilis gram negative bacteria strains Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli and fungal strains such as Candida albicans, C. parapsilosis, C. krusei and C. glabrata using disc diffusion method, determination of Minimum Inhibitory Concentrations (MIC), Minimum Bacterial Concentrations (MBC) and Minimum Fungicidal Concentrations (MFC). The antimicrobial activity for different concentrations like 250 μg, 500 μg and 1000 μg of different solvent extracts of Lannea cormandelica. bacterial strains and recorded in highest mean zones of inhibition ranged from 19.6 mm and Candidal strains and the exhibited the highest mean zones of inhibition ranged from 10.6 mm. Methanol extracts showed the best results as inhibition zones against tested organisms. Results showed also that, the greatest effect was towards Staphylococcus aureus and the lowest was against Candida krusei. The present study reported the great effect of Lannea cormandelica extracts against some of most important pathogens.

scholarly journals Phytochemistry PREPARATION OF DIFFERENT CRUDE EXTRACT AND ANTIMICROBIAL STUDIES OF SPIROGYRA RHIZOPUS

2019 ◽  
pp. 271-274
Author(s):  
DANIEL E ◽  
GIRMA T ◽  
VENKATESAN JAYAKUMAR S
Keyword(s):  
Green Algae ◽  
Crude Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
Discovery Research ◽  
Crude Extracts ◽  
Drug Discovery Research ◽  
Pharmacologically Active

Objective: The importance of this work is to prepare the crude extracts of Spirogyra rhizopus and to study the biological activity of crude extract against four bacterial strains. Materials and Methods: Spirogyra algae collected from Jimma town, and crude extracts were prepared by cold percolation method and sonication method and further analyzed for qualitative phytochemical analysis. The efficacy of crude extracts is tested for bacterial activity by disc-diffusion method. Results: The maximum zone of inhibition shown by the crude extract is compared to standard and control. Among the four extracts, chloroform extract displayed the promising inhibitory action against four bacterial strains. Conclusions: The preliminary study concludes that green algae S. rhizopus is a potential source of pharmacologically active lead molecules. In vitro screening of crude extracts of green algae S. rhizopus shows promising activity against bacterial strains and thus suggests its application in drug discovery research.

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