scholarly journals Gonadal characterization of the Amazonian fish Serrapinnus kriegi (Characidae: Cheirodontinae)

2023 ◽  
Vol 83 ◽  
Author(s):  
G.R. Felicio ◽  
J.G. Cordeiro ◽  
B.P. Dutra-Costa ◽  
C. Maximino ◽  
G.S. Branco ◽  
...  
Keyword(s):  
Cell Formation ◽  
Seminiferous Tubules ◽  
Zona Radiata ◽  
Amazonian Fish ◽  
Asynchronous Development ◽  
Ovarian Morphology ◽  
Amazon Fish ◽  
Reproduction Management ◽  
Spawning Sites

Abstract The knowledge of the testicular and ovarian morphology of a particular fish species is of paramount importance. Such analyze enables the development of studies and techniques aiming the improvement of their reproduction, management, commercialization and even their conservation. This study performed the ovarian and testicular characterization of the ornamental Amazon fish Serrapinnus kriegi. A total of three males and three females had their gonads analyzed by optical microscopy. Females present ovaries filled with oocytes in asynchronous development, indicating partial spawning in the species. Moreover, the micropyle and micropilar cell formation was observed in primary growing oocytes, representing a precocious oocyte development; and the zona radiata in the final vitellogenic oocytes is thicker than other related species, evidencing the development of a better protection to the embryos in function of the waters’ turbulence that characterize it spawning sites in the Amazonian streams. The male specimens’ present anastomosed tubular testes with unrestricted spermatogonia spread along the entire seminiferous tubules. The present data elucidate the dynamic of spermatogenesis and oogenesis of an ornamental Amazonian species, through the description of the male and female germ cells development.

Characterization of the glycoconjugates of boar testis and epididymis

Reproduction ◽  
2000 ◽  
pp. 325-335 ◽  
Author(s):  
A Calvo ◽  
LM Pastor ◽  
S Bonet ◽  
E Pinart ◽  
M Ventura
Keyword(s):  
Ricinus Communis ◽  
Lectin Histochemistry ◽  
Apical Part ◽  
Seminiferous Tubules ◽  
In Situ Characterization ◽  
Intense Staining ◽  
Terminal Galactose ◽  
Boar Spermatozoa

Lectin histochemistry was used to perform in situ characterization of the glycoconjugates present in boar testis and epididymis. Thirteen horseradish peroxidase- or digoxigenin-labelled lectins were used in samples obtained from healthy fertile boars. The acrosomes of the spermatids were stained intensely by lectins with affinity for galactose and N-acetyl-galactosamine residues, these being soybean, peanut and Ricinus communis agglutinins. Sertoli cells were stained selectively by Maackia ammurensis agglutinin. The lamina propria of seminiferous tubules showed the most intense staining with fucose-binding lectins. The Golgi area and the apical part of the principal cells of the epididymis were stained intensely with many lectins and their distribution was similar in the three zones of the epididymis. On the basis of lectin affinity, both testis and epididymis appear to have N- and O-linked glycoconjugates. Spermatozoa from different epididymal regions showed different expression of terminal galactose and N-acetyl-galactosamine. Sialic acid (specifically alpha2,3 neuraminic-5 acid) was probably incorporated into spermatozoa along the extratesticular ducts. These findings indicate that the development and maturation of boar spermatozoa are accompanied by changes in glycoconjugates. As some lectins stain cellular or extracellular compartments specifically, these lectins could be useful markers in histopathological evaluation of diseases of boar testis and epididymis.

Development and characterization of a human marrow stromal cell line that enhances osteoclast-like cell formation.

Endocrinology ◽  
1995 ◽  
Vol 136 (4) ◽  
pp. 1441-1449 ◽  
Author(s):  
S Takahashi ◽  
S V Reddy ◽  
M Dallas ◽  
R Devlin ◽  
J Y Chou ◽  
...  
Keyword(s):  
Cell Line ◽  
Stromal Cell ◽  
Cell Formation ◽  
Marrow Stromal Cell ◽  
Stromal Cell Line

scholarly journals Immunohistochemical Characterization of Spontaneous Sertoli Cell Clusters in the Seminiferous Tubules of C57BL/6J Mice

2015 ◽  
Vol 43 (5) ◽  
pp. 743-746 ◽  
Author(s):  
Akiko Anagawa-Nakamura ◽  
Kochi Kakimoto ◽  
Katsuhiro Miyajima ◽  
Yuzo Yasui ◽  
Yusuke Kemmochi ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Seminiferous Tubules ◽  
Cell Clusters

scholarly journals Morphological aspects of the ovaries of turtle Kinosternon scorpioides raised in captivity

2012 ◽  
Vol 32 (7) ◽  
pp. 667-671 ◽  
Author(s):  
Elba P. Chaves ◽  
Sâmia C.R. Oliveira ◽  
Lianne P.F. Araújo ◽  
Antonia S. Oliveira ◽  
Maria A. Miglino ◽  
...  
Keyword(s):  
Rural Areas ◽  
Rainy Season ◽  
Basal Layer ◽  
Vitelline Membrane ◽  
Zona Radiata ◽  
Morphological Aspects ◽  
Ovarian Morphology ◽  
In Captivity ◽  
Mud Turtle ◽  
The Right

The swear turtle "jurará" (Kinosternon scorpioides) is a mud turtle of the Amazon region exposed to disordering capture in the rural areas of Maranhão, Brazil. Despite its popularity in these areas, little meaningful information regarding the reproductive morphology is currently available, fact that impedes the adoption of policies for preservation of the species. To obtain more information, we studied the ovarian morphology adult jurará females kept in captivity by morphological and morphometric analysis in the dry and rainy season. The results revealed that all females were sexually mature and were in a vitellogenic period. The ovaries are two irregular structures composed by follicles in different stages of development (primary, secondary and tertiary) scattered in a stroma of loose connective highly vascularized tissue. The ovary weight was 6.25±4.23g and 2.27±1.42g, for the right and left one respectively. The gonadosomatic indexes were 2.06% for the dry season and 1.79% for the rainy season. The average of the follicles was 29.83 units per ovary. Microscopically, the mature ovaries revealed a basal layer composed by four cellular layers: the inner and outer theca, stratum granulosum with perivitelline membrane and zona radiata with vitelline membrane. No significant differences were observed in the ovaries either in the dry or wet period.

Characterization of a nuclear receptor for testosterone in seminiferous tubules of mature rat testes

1975 ◽  
Vol 2 (3) ◽  
pp. 171-182 ◽  
Author(s):  
Eppo Mulder ◽  
Marjan J. Peters ◽  
Joan De Vries ◽  
Henk J. Van Der Molen
Keyword(s):  
Nuclear Receptor ◽  
Seminiferous Tubules

scholarly journals Identification and Characterization of Genes Involved in Embryonic Crystal Cell Formation During Drosophila Hematopoiesis

Genetics ◽  
2004 ◽  
Vol 168 (1) ◽  
pp. 325-339 ◽  
Author(s):  
Allison B. Milchanowski ◽  
Amy L. Henkenius ◽  
Maya Narayanan ◽  
Volker Hartenstein ◽  
Utpal Banerjee
Keyword(s):  
Cell Formation ◽  
Crystal Cell ◽  
Identification And Characterization

Acute Exposure to the Neonicotinoid Insecticide Imidacloprid of Zebrafish (Danio rerio) Gonads: A Histopathological Approach

2021 ◽  
Vol 57 ◽  
pp. 23
Author(s):  
Cansu Akbulut
Keyword(s):  
Interstitial Fibrosis ◽  
Periodic Acid ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Periodic Acid Schiff ◽  
Histological Techniques ◽  
Zona Radiata ◽  
Structural Deterioration ◽  
Neonicotinoid Insecticide

Neonicotinoids are the new class of insecticides that are high target specificity to insects. Imidacloprid is a neonicotinoid that is the most widely used insecticide in the world. As a result of its widespread use in agriculture, imidacloprid interferes with the aquatic system and threatens the aquatic environment. In this study, an investigation of the histopathological effects of imidacloprid on zebrafish gonads was aimed. Zebrafish were exposed to 9.5 mg/L, 19 mg/L, and 38 mg/L of imidacloprid for 5 days, considering the 96 h LC50 value. After dissecting the gonadal tissues, routine histological techniques were applied, and the tissues were stained with Periodic Acid-Schiff (PAS), Toluidine Blue (TB), and Haematoxylin and Eosin (H&E). Sections were examined under a light microscope. While normal gonad histology was observed in the control group, histopathological alternations such as degeneration and union in the seminiferous tubules, hypertrophy in spermatogenic and Leydig cells, and interstitial fibrosis were detected in testicular tissue of the experimental groups. In the ovarian tissues of the experimental groups, structural deterioration in oocytes, autolysis, increase in the number of atretic oocytes, vacuolization in cortical alveoli, thickening and curling in the zona radiata, and opening in the perifollicular layers were detected.

Development and characterization of microsatellite loci in Amazonian dwarf cichlids Apistogramma spp. (Perciformes: Cichlidae): Uncovering geological influence on Amazonian fish population

2017 ◽  
Vol 33 (6) ◽  
pp. 1196-1199 ◽  
Author(s):  
C. S. S. Leitão ◽  
C. H. A. Santos ◽  
É. M. S. Souza ◽  
G. C. C. Vilarinho ◽  
M. N. Paula-Silva ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Fish Population ◽  
Amazonian Fish

Molecular characterization of a germ-cell-specific antigen, TEX101, from mouse testis

Zygote ◽  
2006 ◽  
Vol 14 (3) ◽  
pp. 201-208 ◽  
Author(s):  
Hong Jin ◽  
Hiroshi Yoshitake ◽  
Hiroki Tsukamoto ◽  
Mai Takahashi ◽  
Miki Mori ◽  
...  
Keyword(s):  
Germ Cell ◽  
Biochemical Characterization ◽  
Specific Antigen ◽  
Cell Formation ◽  
Gonad Development ◽  
Enzyme Treatment ◽  
Molecular Characteristics ◽  
Specific Marker ◽  
Morphological Studies

SummaryTEX101, a glycoprotein we recently identified, is primarily characterized as a unique germ-cell-specific marker protein that shows sexually dimorphic expression during mouse gonad development. Based on data obtained from molecular biological as well as immuno-morphological studies, we believe this molecule may play a role in the process underlying germ cell formation. However, many points remain unclear as the molecular characteristics and its physiological functions are far from being completely understood. To clarify the molecular basis of TEX101, we herein report a further biochemical characterization of the molecule using testicular Triton X-100 extracts from mice. Deglycosylation studies using endoglycohydrolases that delete N-linked oligosaccharides (OS) from the molecule show that TEX101 is highly (approximately 47%) N-glycosylated. All potential N-glycosylation sites within TEX101 are glycosylated and most of these sites are occupied by endoglycosidase F2-sensitive biantennary complex type OS units. In addition, an extremely low population among TEX101 possesses only endoglycosidase H-sensitive hybrid type OS units. In studies using phosphatidylinositol-specific phospholipase C against native testicular cells or TEX101 transfectant, the enzyme treatment caused major reduction of the TEX101 expression on the cell, suggesting that TEX101, at least in part, is expressed as a glycosylphosphatidylinositol-anchored protein. Taken together, these findings will help elucidate the molecular nature of TEX101, a marker molecule that appeared on germ cells during gametogenesis.

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