scholarly journals Evaluation of three alum-precipitated Aeromonas hydrophila vaccines administered to Labeo rohita, Cirrhinus mrigala and Ctenopharyngodon idella: immunokinetics, immersion challenge and histopathology

2023 ◽  
Vol 83 ◽  
Author(s):  
F. Sughra ◽  
M. Hafeez-ur Rahman ◽  
F. Abbas ◽  
I. Altaf
Keyword(s):  
Immune Response ◽  
Aeromonas Hydrophila ◽  
Labeo Rohita ◽  
Local Strain ◽  
Ctenopharyngodon Idella ◽  
Fixed Dose ◽  
Control Group ◽  
Cirrhinus Mrigala ◽  
Wide Range ◽  
Antibody Titers

Abstract Aeromonas hydrophila is a cause of infectious disease outbreaks in carp species cultured in South Asian countries including Pakistan. This bacterium has gained resistance to a wide range of antibiotics and robust preventive measures are necessary to control its spread. No prior use of fish vaccines has been reported in Pakistan. The present study aims to develop and evaluate inactivated vaccines against local strain of A. hydrophila in Pakistan with alum-precipitate as adjuvant. The immunogenic potential of vaccine was evaluated in two Indian major carps (Rohu: Labeo rohita, Mori: Cirrhinus mrigala) and a Chinese carp (Grass carp: Ctenopharyngodon idella). Fish were vaccinated intraperitoneally followed by a challenge through immersion. Fish with an average age of 4-5 months were randomly distributed in three vaccinated groups with three vaccine concentrations of 108, 109 and 1010 colony forming unit (CFU)/ml and a control group. Fixed dose of 0.1ml was applied to each fish on 1st day and a booster dose at 15 days post-vaccination (DPV). Blood samples were collected on 14, 28, 35, 48 and 60 DPV to determine antibody titers in blood serum using compliment fixation test (CFT). Fish were challenged at 60 DPV with infectious A. hydrophila with 108 CFU/ml through immersion. Significantly higher levels of antibody titers were observed from 28 DPV in all vaccinated groups as compared to those in the control group. In challenge experiment the average RPS (relative percent survivability) was 71% for groups vaccinated with 109 and 1010 CFU/ml and 86% for 108 CFU/ml. Vaccine with 108 CFU/ml induced highest immune response followed by 109 and 1010 CFU/ml. The immune response of L. rohita and C. idella was better than that of C. mrigala. In general, normal histopathology was observed in different organs of vaccinated fish whereas minor deteriorative changes were found in fish vaccinated with higher concentrations of the vaccine.

scholarly journals In vivo assessment of oxidative stress in heart cells of Cirrhinus mrigala (Hamilton, 1822) challenged with sublethal doses of Aeromonas hydrophila

2019 ◽  
Vol 66 (4) ◽  
Author(s):  
Sriparna Datta Ray ◽  
Sumit Homechaudhuri
Keyword(s):  
Oxidative Stress ◽  
Immune Response ◽  
Aeromonas Hydrophila ◽  
Heart Cells ◽  
Apoptotic Cells ◽  
Fish Heart ◽  
Cirrhinus Mrigala ◽  
Immune Biomarkers ◽  
Significant Difference

Experiment was performed to study the effect of Aeromonas hydrophila MTCC 646 on the heart of Cirrhinus mrigala (Hamilton, 1822) . The in vivo innate immune response was also monitored in these fishes. Fishes were inoculated with 3 asymptomatic doses (2x105, 2x106 and 2x107 CFU ml-1) intraperitoneally (i.p.) @ 0.5 ml 100 g-1. Dose dependent pattern of change was observed in the immune biomarkers chosen for the study. Histopathology revealed endocarditis and cell death identified as apoptosis. The percentage contribution of late apoptotic cells was 0.36% after 7 days in fish heart exposed to the highest dose of bacteria which was reduced to 0.01% after 30 days. Oxidative stress and dosage were directly proportional. The oxidised dichloro fluorescein peak was about 4.05 fold more than the control samples, after 7 days with respect to the highest dose and immune responses helped to decrease the mean peak to 1.02 after 30 days, signifying recovery of phagocytic activity, which is a part of the reticulo-endothelial system. This work presents an insight into the immune response and the results analysed statistically show that there was significant difference between percent population of early apoptotic cells (p<0.05) collected after 7 days and 30 days duration with respect to all the three doses.

scholarly journals Genome-Wide Differential Expression Profiling of Pulmonary circRNAs Associated With Immune Reaction to Pasteurella multocida in Goats

2021 ◽  
Vol 8 ◽  
Author(s):  
Qiaoling Chen ◽  
Zhenxing Zhang ◽  
Si Chen ◽  
Jie Chen ◽  
Yiwen Cheng ◽  
...  
Keyword(s):  
Immune Response ◽  
Messenger Rna ◽  
Pasteurella Multocida ◽  
Clinical Signs ◽  
Infected Group ◽  
Differentially Expressed ◽  
Control Group ◽  
Small Rna Sequencing ◽  
Nasal Discharge ◽  
Wide Range

Pasteurella multocida is a highly versatile pathogen that infects a wide range of animals, including goats, causing pneumonia and hemorrhagic septicemia. Circular RNA (circRNA) is a type of non-coding RNA that plays an important role in regulating cellular metabolism. However, whether and how circRNA is involved in regulating immune responses in the goat lung has not been reported. Thus, this study was designed to examine the function of circRNA in goats infected with Pasteurella multocida. Goats were assigned into one of two groups: an uninfected control group (CK) and an infected group challenged with P. multocida. Compared with the CK group, which remained healthy, the infected goats showed clinical signs of infection, including depression, cough, nasal discharge, and dyspnea, along with elevated body temperature and lesions in the lung. Whole-transcriptome sequencing and small RNA sequencing were then performed using lung samples from goats from each group. A total of 138 circRNA, 56 microRNAs (miRNA), and 2,673 messenger RNA (mRNA) molecules were significantly differentially expressed in the P. multocida-infected group compared with the CK group. Randomly selected differentially expressed circRNA, miRNA, and mRNA molecules (n = 5 per group) were then validated by quantitative reverse-transcriptase polymerase chain reaction analysis. Gene ontology (GO) analysis of the source genes indicated that six immune-related terms were enriched among the differentially expressed cirRNA molecules, including inflammatory response, immune effector process, cell activation involved in immune response, cytokine-mediated signaling pathway, response to endogenous stimulus, and immune response. The corresponding circRNA molecules were then selected for construction of a competitive endogenous RNA network to identify networks that may be involved in the immune response to P. multocida infection. The results indicated that P. multocida HN01 may cause pneumonia and stimulate an immune response in goats via regulation of circRNA expression. This study presents the first comprehensive circRNA profile in response to P. multocida infection in goats, thus, providing a basis for understanding the function of circRNA in the host immune response to P. multocida infection.

The Immune Response of Children with Chronic Lymphocytic Thyroiditis to a Viral Challenge

PEDIATRICS ◽  
1966 ◽  
Vol 37 (6) ◽  
pp. 1009-1011
Author(s):  
Allen W. Root ◽  
Alfred M. Bongiovanni ◽  
Wanlter R. Eberlein
Keyword(s):  
Immune Response ◽  
Adenovirus Type ◽  
Control Group ◽  
Chronic Lymphocytic Thyroiditis ◽  
Viral Challenge ◽  
Lymphocytic Thyroiditis ◽  
Control Subjects ◽  
Antibody Titers ◽  
The Mean ◽  
Adenovirus Vaccine

Twenty-eight children with CLT and 22 control subjects were immunized with an adenovirus vaccine. The mean anti-Adenovirus Type 7 titer after vaccination in the group with CLT was significantly less than that in the control group. No significant changes occurred in anti-thyrold antibody titers consequent to the immunization. Patients with CLT had higher mean anti-A and anti-B titers than did control subjects. It is concluded that patients with CLT do not hyperrespond to a viral challenge.

Effect of cellular products of potential probiotic bacteria on the immune response of Labeo rohita and susceptibility to Aeromonas hydrophila infection

2015 ◽  
Vol 46 (2) ◽  
pp. 716-722 ◽  
Author(s):  
Sib Sankar Giri ◽  
Shib Sankar Sen ◽  
Cheng Chi ◽  
Hyoun Joong Kim ◽  
Saekil Yun ◽  
...  
Keyword(s):  
Immune Response ◽  
Aeromonas Hydrophila ◽  
Labeo Rohita ◽  
Probiotic Bacteria
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