Significant production of vanillin and in vitro amplification of ech gene in local bacterial isolates

Abstract Vanillin is the major component which is responsible for flavor and aroma of vanilla extract and is produced by 3 ways: natural extraction from vanilla plant, chemical synthesis and from microbial transformation. Current research was aimed to study bacterial production of vanillin from native natural sources including sewage and soil from industrial areas. The main objective was vanillin bio-production by isolating bacteria from these native sources. Also to adapt methodologies to improve vanillin production by optimized fermentation media and growth conditions. 47 soil and 13 sewage samples were collected from different industrial regions of Lahore, Gujranwala, Faisalabad and Kasur. 67.7% bacterial isolates produced vanillin and 32.3% were non-producers. From these 279 producers, 4 bacterial isolates selected as significant producers were; A3, A4, A7 and A10. These isolates were identified by ribotyping as A3 Pseudomonas fluorescence (KF408302), A4 Enterococcus faecium (KT356807), A7 Alcaligenes faecalis (MW422815) and A10 Bacillus subtilis (KT962919). Vanillin producers were further tested for improved production of vanillin and were grown in different fermentation media under optimized growth conditions for enhanced production of vanillin. The fermentation media (FM) were; clove oil based, rice bran waste (residues oil) based, wheat bran based and modified isoeugenol based. In FM5, FM21, FM22, FM23, FM24, FM30, FM31, FM32, FM34, FM35, FM36, and FM37, the selected 4 bacterial strains produced significant amounts of vanillin. A10 B. subtilis produced maximum amount of vanillin. This strain produced 17.3 g/L vanillin in FM36. Cost of this fermentation medium 36 was 131.5 rupees/L. This fermentation medium was modified isoeugenol based medium with 1% of isoeugenol and 2.5 g/L soybean meal. ech gene was amplified in A3 P. fluorescence using ech specific primers. As vanillin use as flavor has increased tremendously, the bioproduction of vanillin must be focused.

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Isolation and evaluation of rhizobacteria against Ralstonia solanacearum the incitant of bacterial wilt of tomato

INTERNATIONAL JOURNAL OF PLANT PROTECTION ◽

10.15740/has/ijpp/13.2/195-199 ◽

2020 ◽

Vol 13 (2) ◽

pp. 195-199

Author(s):

C.S. Karibasappa ◽

Yogendra Singh

Keyword(s):

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Inhibition Zone ◽

Crop Plants ◽

The Novel ◽

Bacterial Isolates ◽

Bacterial Strains ◽

Biocontrol Activity ◽

Highly Effective

Bacterial wilt caused by Ralstonia solanacearum is the world’s most economically important destructive disease of crop plants. In the current study, we aimed to evaluate the novel bacterial isolates from tomato rhizosphere for biocontrol of Ralstonia solanacearum. One eighty bacterial strains were isolated from the tomato rhizosperic soils collected from different regions of Uttarakhand state and evaluated for their biocontrol activity against R. solanacearum under in vitro conditions. Among them, six isolates were found to be highly effective in inhibiting the growth of R. solanacearum. The isolate GP2NA8 produced the highest inhibition zone followed by that of GP1NA2 and GP3NA6.

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Compost bacteria as potential agents in biological plant control

Progress in Plant Protection ◽

10.14199/ppp-2021-034 ◽

2021 ◽

Vol 61 (4) ◽

pp. 319-326

Keyword(s):

Pathogenic Fungi ◽

16S Rrna Gene Sequencing ◽

Alcaligenes Faecalis ◽

Diffusion Method ◽

Rrna Gene ◽

Bacterial Strains ◽

Fungistatic Activity ◽

Activity Spectrum ◽

Rrna Gene Sequencing

The assumptions of integrated pest management put great emphasis on the development of non-chemical methods which increases the interest in biological methods and the search for microorganisms that would be an alternative to the most frequently used fungicides. The aim of the experiments was the isolation of the compost bacteria, in vitro determination of their fungistatic activity against some pathogenic fungi of the genus Fusarium, Alternaria, Sclerotinia, Botrytis, Rhizoctonia and Pythium and identification of selected isolates. From the backyard compost, 44 bacterial strains were isolated and assessed for the fungistatic properties by the well diffusion method. The obtained results allowed for the selection of 12 isolates of compost bacteria, characterised by the broadest and the strongest fungistatic activity spectrum against tested fungi. Identification of bacterial isolates by: MALDI-TOF mass spectrometry and 16S rRNA gene sequencing methods showed their belonging to the species Bacillus subtilis, Alcaligenes faecalis, Stenotrophomonas maltophilia and Serratia liquefaciens.

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Technological Potential ofLactobacillusStrains Isolated from Fermented Green Olives:In VitroStudies with Emphasis on Oleuropein-Degrading Capability

The Scientific World JOURNAL ◽

10.1155/2016/1917592 ◽

2016 ◽

Vol 2016 ◽

pp. 1-11 ◽

Cited By ~ 13

Author(s):

Massimo Iorizzo ◽

Silvia Jane Lombardi ◽

Vincenzo Macciola ◽

Bruno Testa ◽

Giuseppe Lustrato ◽

...

Keyword(s):

Optimal Growth ◽

Growth Conditions ◽

Medium Composition ◽

Technological Properties ◽

Bacterial Strains ◽

Good Tolerance ◽

Lactobacillus Pentosus ◽

Technological Potential ◽

Nutrient Rich Medium

Technological properties of two strains ofLactobacillus plantarum(B3 and B11) and one ofLactobacillus pentosus(B4), previously isolated from natural fermented green olives, have been studiedin vitro. Acidifying ability, salt, temperature, and pH tolerances of all strains were found in the range reported for similar strains produced in Italy and optimal growth conditions were found to be 6.0–8.0 pH, 15–30°C temperature, and less than 6% NaCl. Moreover, all strains showed very good tolerance to common olive phenol content (0.3% total phenol) and high oleuropein-degrading capability. It was found that medium composition affected the bacterial oleuropein degradation. B11 strain grown in a nutrient-rich medium showed a lower oleuropein-degrading action than when it was cultivated in nutrient-poor medium. Furthermore, enzymatic activity assays revealed that oleuropein depletion did not correspond to an increase of hydroxytyrosol, evidencing that bacterial strains could efficiently degrade oleuropein via a mechanism different from hydrolysis.

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Microbial Purification of Postfermentation Medium after 1,3-PD Production from Raw Glycerol

BioMed Research International ◽

10.1155/2013/949107 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 4

Author(s):

Daria Szymanowska-Powałowska ◽

Joanna Piątkowska ◽

Katarzyna Leja

Keyword(s):

Lactic Acid ◽

Carbon Source ◽

Organic Acids ◽

Butyric Acid ◽

Bacillus Licheniformis ◽

Fermentation Medium ◽

Alcaligenes Faecalis ◽

Bacterial Strains ◽

Chemical Product ◽

Raw Glycerol

1,3-Propanediol (1,3-PD) is an important chemical product which can be used to produce polyesters, polyether, and polyurethanes. In the process of conversion of glycerol to 1,3-PD byClostridiumlarge number of byproducts (butyric, acetic and lactic acid) are generated in the fermentation medium. The aim of this work was to isolate bacteria strains capable of the utilization of these byproducts. Screening of 30 bacterial strains was performed using organic acids as carbon source. Selected isolates were taxonomically characterized and identified asAlcaligenes faecalisandBacillus licheniformis. The most active strains,Alcaligenes faecalisJP1 andBacillus licheniformisJP19, were able to utilize organic acids almost totally. Finally, it was find out that by the use of coculture (C. butyricumDSP1 andA. faecalisJP1) increased volumetric productivity of 1,3-PD production (1.07 g/L/h) and the yield equal to 0.53 g/g were obtained in bioreactor fermentation. Moreover, the only by-product present was butyric acid in a concentration below 1 g/L.

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Indigenous Bacillus paramycoides and Alcaligenes faecalis: potential solution for the bioremediation of wastewaters

10.1101/2020.05.20.105940 ◽

2020 ◽

Author(s):

Aneeba Rashid ◽

Safdar A. Mirza ◽

Ciara Keating ◽

Sikander Ali ◽

Luiza C. Campos

Keyword(s):

Low Cost ◽

Growth Conditions ◽

Alcaligenes Faecalis ◽

Hospital Wastewater ◽

Bacterial Strains ◽

The Sustainable Development ◽

Rdna Sequencing ◽

Wide Range ◽

Wastewater Samples ◽

Sustainable Development Goal 6

AbstractFarmers near towns and cities are using wide range of untreated wastewaters for crop irrigation in Pakistan due to severe freshwater shortage. The present study aimed to treat different types of wastewater including domestic, hospital, textile, pharmaceutical and mixed wastewaters using indigenous bacterial isolates to remove contaminants and render these wastewaters safer for irrigation. 37 bacterial strains were isolated from the 5 wastewater samples collected from different sites in Lahore, Pakistan. Under optimum growth conditions, the isolates D6, D7 and P1 showed maximum decolourisation potential of 96, 96, 93 %, respectively against hospital wastewater. GCMS analysis of the untreated hospital wastewater confirmed the presence of pharmaceutic pollutants i.e. Phenol, Salicylic acid, Caffeine, Naproxen, Octadecene and Diazepam. These organic compounds were biodegraded into derivate Ticlopidine in the case of isolate D6, derivatives Tetradecene and Griseofulvin in the case of isolate D7, and derivatives Lidocaine and Butalbital in the case of isolate P1. 16S rDNA sequencing was used to identify these isolates. Isolates D6 and D7 showed 100 and 99.86 % homology to Bacillus paramycoides, a novel strain from Bacillus cereus group (Liu et al., 2017). Isolate P1 showed 97.47 % homology to Alcaligenes faecalis. These strains therefore could represent a low-cost and low-tech alternative to bioremediate complex wastewaters prior to irrigation to support the achievement of the Sustainable Development Goal 6 - clean water and sanitation in Pakistan.

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Enhanced production of β- glucosidase by locally isolated fungal strain employing submerged fermentation

Bioscience Journal ◽

10.14393/bj-v35n5a2019-47943 ◽

2019 ◽

Vol 35 (5) ◽

Cited By ~ 1

Author(s):

Roheena Abdullah ◽

Sobia Nazir Chudhary ◽

Afshan Kaleem ◽

Mehwish Iqtedar ◽

Kinza Nisar ◽

...

Keyword(s):

Submerged Fermentation ◽

Wide Spectrum ◽

Nitrogen Sources ◽

Fermentation Medium ◽

Fungal Strain ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Enhanced Production ◽

Different Sources ◽

Fermentation Media

β-glucosidase has wide spectrum of biotechnological applications in different industries including food, textile, laundry detergents, pulp and paper, pharmaceutical and biofuel industry. The present investigation related to isolation, screening, and process optimization of fungal strain for enhanced production of β-glucosidase (BGL). For this purpose, different fungal stains were isolated from different sources including soil, fruits, bark of tree as well as from the compost. The screening of fungal strain for BGL production was carried out via submerged fermentation. All the tested strains were identified on the basis of micro and macroscopic features. The fungal strain having greater ability for BGL synthesis among tested ones was identified as Aspergillus niger and given the code SBT-15. The process parameter including fermentation media, temperature, pH, rate of fermentation, carbon and nitrogen sources, volume of media were optimized. Five different fermentation media were evaluated M3 medium gave maximum production. The optimal conditions for BGL production was 72 hours of incubation at 40°C, pH 6 and 50 ml fermentation medium. Glucose (1%) and ammonium sulphate (3%) were optimized as best carbon and nitrogen sources, respectively.

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In Vitro Study of Butyric Acid Deodorization Potential by Indigenously Constructed Bacterial Consortia and Pure Cultures from Pit Latrine Fecal Sludge

Sustainability ◽

10.3390/su12125156 ◽

2020 ◽

Vol 12 (12) ◽

pp. 5156

Author(s):

John Bright Joseph Njalam’mano ◽

Evans Martin Nkhalambayausi Chirwa ◽

Refilwe Lesego Seabi

Keyword(s):

Butyric Acid ◽

In Vitro Study ◽

Bacterial Consortium ◽

Growth Conditions ◽

Bacterial Strains ◽

Bacterial Consortia ◽

Fecal Sludge ◽

Pure Cultures ◽

Pit Latrine

The present study aims at developing an efficient bacterial consortium to biodegrade butyric acid, one of the odor-causing compounds that contribute significantly to pit latrine malodors. Six bacterial strains isolated from pit latrine fecal sludge were selected for the study. Nineteen bacterial consortia of different combinations were artificially constructed. The individual bacterial strains and bacterial consortia were compared by culturing in mineral salt medium supplemented with 1000 mg/L butyric acid as a sole carbon and energy source at pH 7, 30 °C, and 110 rpm under aerobic growth conditions. A co-culture of Serratia marcescens and Bacillus cereus was an effective bacterial consortium compared to individual component bacterial strains and other bacterial consortia, in which 1000 mg/L butyric acid was completely degraded within 16 h of incubation. A temperature of 30 °C and pH 7 were found to be optimum for the maximum degradation for both S. marcescens and B. cereus. The inoculation sizes of 2.0 and 2.5 were optimal for the maximum degradation for B. cereus and S. marcescens, respectively. The study provides insights that will be of substantial help in the development of effective biological treatment technologies for pit latrine odor to change the pit latrine user community’s and would be users’ perception of pit latrines.

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Characterization of plant growth-promoting bacteria inhabiting Vriesea gigantea Gaud. and Tillandsia aeranthos (Loiseleur) L.B. Smith (Bromeliaceae)

Biota Neotropica ◽

10.1590/s1676-06032013000300010 ◽

2013 ◽

Vol 13 (3) ◽

pp. 80-85 ◽

Cited By ~ 8

Author(s):

Adriana Giongo ◽

Anelise Beneduzi ◽

Kelsey Gano ◽

Luciano Kayser Vargas ◽

Laura Utz ◽

...

Keyword(s):

Phosphate Solubilization ◽

Plant Growth Promoting Bacteria ◽

Water Tank ◽

Bacterial Isolates ◽

Bacterial Strains ◽

H Gene ◽

Plant Growth Promoting ◽

High Nutrient Concentration ◽

Water Tanks

Microorganisms that live inside and around a plant can supply it with essential substances, such as phytohormones and essential nutrients. The present investigation aimed to isolate and characterize the phyllosphere, the endophytic, and the water tank bacteria associated with Vriesea gigantea and Tillandsia aeranthos. The bacteria were tested for siderophore and indole-3-acetic acid (IAA) production, phosphate solubilization, and presence of the nif H gene. Genetic diversity of the bacterial isolates was evaluated by rep-PCR. Sixty-eight bacterial strains were isolated from 3 different microhabitats of V. gigantea and from 2 microhabitats of T. aeranthos bromeliad plants. Gram-positive, spore-forming bacilli comprised most bacterial isolates. All isolates produced IAA in vitro in presence of very low amounts of tryptophan. More than 70% of the evaluated bacteria presented the ability of siderophore production and phosphate solubilization, and possessed the nif H gene. It was not possible to distinguish well-defined groups of isolates based on the bromeliad species and microhabitat they inhabit using genetic characterization by rep-PCR. Water tanks presented the most abundant diversity compared with phyllosphere and endophytes, probably due to the high nutrient concentration, which promotes an ideal environment for complex microbial communities.

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Antibacterial Activity of Some Nano Particles on Antibiotic-resistant Bacterial Pathogens from the Air of Operation Theatre

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2019/v29i230231 ◽

2019 ◽

pp. 1-8

Author(s):

Nazish Mazhar Ali ◽

Saiqa Andleeb ◽

Abdullah Mazhar ◽

Bushra Mazhar ◽

Sehar Zahid

Keyword(s):

Antibacterial Activity ◽

Ferric Oxide ◽

Bacterial Pathogens ◽

Research Work ◽

Bacterial Pathogen ◽

Growth Conditions ◽

Nano Particles ◽

Bacterial Isolates ◽

Bacterial Strains ◽

Operation Theatre

The current research work was carried out to find the antibacterial activity of some nano particles against bacterial pathogens isolated from the air of operation theatre of Mayo hospital, Lahore, Pakistan. Three pathogenic bacterial strains were isolated, namely A1, A2, A3. Molecular characterisation, optimum growth conditions and antibiotic resistance of bacterial isolates were checked. The antibiotics used in this study were Amoxycillin, Cefepime and Ampicillin. Nano particles were used in methanolic solutions (mg/ml). Nano particles included ferric oxide, Zinc oxide and Silver Oxide. Results showed A3 was resistant to all antibiotics. Other strains showed sensitivity and resistance to these three antibiotics. All nano particles showed antibacterial activity against pathogenic bacterial isolates. Maximum zone of inhibition of 1 cm was formed when used Ferric oxide against the A1 bacterial pathogen. Optimum temperature was 37°C while the optimum pH was 7. These bacterial pathogens were identified by ribotyping as Staphylococcus aureus (A1), Pseudomonas aeruginosa (A2) and Streptococcus pyogenes (A3).

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Organic Acid Profiles of Phosphate Solubilizing Bacterial Strains in the Presence of Different Insoluble Phosphatic Sources Under In vitro Buffered Conditions

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.15.2.59 ◽

2021 ◽

Author(s):

Chandandeep Kaur ◽

Govindan Selvakumar ◽

Kaushal Kishore Upreti

Keyword(s):

Organic Acids ◽

Organic Acid ◽

Gluconic Acid ◽

High Performance ◽

Culture Media ◽

Soil Conditions ◽

Bacterial Isolates ◽

Bacterial Strains ◽

Phosphate Solubilizing

The production of weak organic acids by microorganisms has been attributed as the prime reason for the solubilization of insoluble phosphates under both in vitro and soil conditions. Literature seems to be heavily biased towards gluconic acid production by microbes and its subsequent release into the environment as the key factor responsible for phosphate solubilization. This has found credibility since gluconic acid being a product of the Kreb’s cycle is often detected in large quantities in the culture media, when assayed under in vitro conditions. In the present work, the organic acid profiles of four elite phosphate solubilising isolates were determined in the presence of different insoluble sources of phosphates, under in vitro buffered culture conditions by HPLC (High-Performance Liquid Chromatography). While most previous studies did not use a buffered culture media for elucidating the organic acid profile of phosphate solubilizing bacterial isolates, we used a buffered media for estimation of the organic acid profiles. The results revealed that apart from gluconic acid, malic acid is produced in significant levels by phosphate solubilizing bacterial isolates, and there seems to be a differential pattern of production of these two organic acids by the isolates in the presence of different insoluble phosphate sources.

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