Toxicity evaluation of Dolichandrone serrulata flower extract on vital and reproductive organs in adult male rats

Abstract Although Dolichandrone serrulata flower (DSF) aqueous extract has been shown to possess pharmacological properties, its systemic toxicity has still to be evaluated. The present study aimed to investigate the sub-chronic toxicity effect of DSF extract on biochemical parameters and histological structures of liver, kidney, testis, and epididymis plus vas deferens. Adult male rats were administered DSF at 100, 300, and 600 mg/kgBW via oral gavage for 48 consecutive days while control rats received distilled water. At the end of the experiment, blood, liver, kidney, testis, and epididymis plus vas deferens samples were collected to determine any changes to serum biochemical components including ALT, ALP, and creatinine levels and histological structures. The results revealed no significant difference in body weight and food or water consumption between control and the DSF-treated groups. It was found that DSF significantly increases the weight of epididymis plus vas deferens, while the kidney and liver showed a decrease in the high dose group (P value < 0.05). Histological changes in these vital and reproductive tissues including fibrosis were not observed after administration but ALT, ALP, and creatinine levels were significantly altered in the treated groups (P value < 0.05). These altered levels, however, were still within normal ranges. In conclusion, these findings demonstrated that D. serrulata flower extract had no sub-chronic toxicity on vital and reproductive structures but slightly altered some liver and kidney functions.

Download Full-text

A Single High Dose of Methamphetamine Reduces Monoamines and Impairs Egocentric and Allocentric Learning and Memory in Adult Male Rats

Neurotoxicity Research ◽

10.1007/s12640-018-9871-9 ◽

2018 ◽

Vol 33 (3) ◽

pp. 671-680 ◽

Cited By ~ 10

Author(s):

Arnold Gutierrez ◽

Michael T. Williams ◽

Charles V. Vorhees

Keyword(s):

Learning And Memory ◽

Adult Male ◽

Male Rats ◽

High Dose ◽

Single High Dose

Download Full-text

Ultrastructural changes in the reproductive organs of adult male rats after chronic soy diet

Reproductive Toxicology ◽

10.1016/j.reprotox.2013.06.054 ◽

2013 ◽

Vol 41 ◽

pp. 30

Author(s):

Paraskevi Papaioannidou ◽

Theodora Papamitsou ◽

Ioannis Makaronidis ◽

Ioannis Kyriakidis ◽

Ioannis Papaioannidis ◽

...

Keyword(s):

Adult Male ◽

Reproductive Organs ◽

Male Rats ◽

Ultrastructural Changes

Download Full-text

197 DIFFERENTIAL EFFECTS OF FLUTAMIDE AND DI-(2-ETHYLHEXYL) PHTHALATE ON MALE REPRODUCTIVE ORGANS IN A RAT MODEL

Reproduction Fertility and Development ◽

10.1071/rdv21n1ab197 ◽

2009 ◽

Vol 21 (1) ◽

pp. 197

Author(s):

T. T. B. Vo ◽

E.-M. Jung ◽

M.-G. Back ◽

V. H. Dang ◽

K.-C. Choi ◽

...

Keyword(s):

Sex Determination ◽

Microarray Analysis ◽

Expression Patterns ◽

Reproductive Organs ◽

Male Rats ◽

Seminiferous Tubules ◽

High Dose ◽

Total N ◽

Male Reproductive Organs ◽

Ethylhexyl Phthalate

Endocrine disruptors (EDs) with androgenic and anti-androgenic effects may alter reproductive function by binding to androgenic receptors (AR) and inducing or modulating AR-dependent responses in the male reproductive system. However, the molecular mechanism(s) underlying these events remains unclear. Thus, in the present study, we elucidated the prenatal effects of maternal testosterone propionate (TP), flutamide (Flu), and di-(2-ethylhexyl) phthalate (DEHP) on male reproductive organs of newborn rats. Pregnant Sprague-Dawley (n = 32 in total, n = 8/each group) rats were treated with these compounds at gestation days 11 to 21, and newborn males (n = 154 in total) were euthanized at post-neonatal day (PND) 63. Interestingly, maternal exposure to Flu or DEHP caused fluctuations in the neonatal levels of serum testosterone (T) and luteinizing hormone (LH). Serum T and LH were up-regulated by Flu, but these hormones were down-regulated by DEHP. The anogenital distances (AGD) of male newborns were detected at PND 1, 21, and 63. Male rats treated prenatally with DEHP (100 mg kg–1 mother’s body weight) or Flu showed an AGD shorter than that of control rats. At PND 63, the sperm concentration, viability, and mobility were reduced in the maternal DEHP and Flu-treated groups. The numbers of seminiferous tubules were reduced in the Flu- and DEHP-treated offspring when compared with vehicle- and TP-treated groups, and the tubules of the testes at PND 63 were disrupted by a high dose of Flu. In addition, we examined differential gene expression patterns in the testes by microarray analysis following ED exposure, particularly in sex determination-related genes. Significantly distinct expressions of sex determination-related genes were observed in the testes by microarray analysis following treatments with different types of EDs in this study. Although Flu and DEHP are considered to be identical with regard to their anti-androgenic effects, their effects on developing male reproductive organs were distinct, suggesting that Flu competes with endogenous T, while DEHP influences a different step in androgenesis.

Download Full-text

Expression of Nerve Growth Factor (NGF), and Its Receptors TrkA and p75 in the Reproductive Organs of the Adult Male Rats

ZOOLOGICAL SCIENCE ◽

10.2108/zsj.22.933 ◽

2005 ◽

Vol 22 (8) ◽

pp. 933-937 ◽

Cited By ~ 16

Author(s):

ChunMei Li ◽

Gen Watanabe ◽

Qiang Weng ◽

WanZhu Jin ◽

Chie Furuta ◽

...

Keyword(s):

Nerve Growth Factor ◽

Growth Factor ◽

Adult Male ◽

Reproductive Organs ◽

Male Rats ◽

Nerve Growth

Download Full-text

Effect of Giving Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract to Epithelialization In Incision Wound of White Rats (Rattus Norvegicus)

Jurnal Ilmiah Keperawatan STIKES Hang Tuah Surabaya ◽

10.30643/jiksht.v12i1.37 ◽

2019 ◽

Vol 12 (1) ◽

Author(s):

Idola Perdana Sulistyoning Suharto

Keyword(s):

Treatment Group ◽

Rattus Norvegicus ◽

Male Rats ◽

Control Group ◽

P Value ◽

Control Group Design ◽

White Rats ◽

Significant Difference ◽

Phaleria Macrocarpa ◽

Incision Wound

The research purpose was to analysis effect of giving mahkota dewa fruits (Phaleria macrocarpa) extract to epithelialization in incision wound of white rats (Rattus norvegicus). The method was randomized posted-only control group design. There were 30 male rats (Rattus norvegicus) grouped on control and treatment group. Control group divided into three groups (KK1, KK2, KK3) and also treatment group divided into three groups (KP1, KP2, KP3). Control group just given CMC 1% peroral without mahkota dewa fruits extract, the treatment group given mahkota dewa fruits extract 22.5 mg/kg body weight. The data was analyzed by Kruskall Wallis. Based on Kruskall Wallis test, obtained result that there was a significant difference (p<0.05) epithelialization variable with p value p = 0.000 between control and treatment group. And based on One-way Anova test, obtained result that there was a significant difference (p<0.05) with p value p =0.000 between control and treatment group. The conclusion of this research was giving mahkota dewa fruits (Phaleria macrocarpa) extract can increase epithelialization in incision wound of white rats (Rattus norvegicus). Keywords : Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract, Epithelialization, Incision Wound

Download Full-text

Duration-dependent effects of high dose of phthalate exposure on semen quality in adult male rats

JBRA Assisted Reproduction ◽

10.5935/1518-0557.20210033 ◽

2021 ◽

Author(s):

Victor Emojevwe ◽

Alexander O Naiho ◽

Eze K Nwangwa ◽

Mega O Oyovwi ◽

Kenneth K Anachuna ◽

...

Keyword(s):

Adult Male ◽

Semen Quality ◽

Male Rats ◽

High Dose ◽

Phthalate Exposure

Download Full-text

Vascular Rehabilitation Benefits of Tribulus Terrestris (TT), Taurine and High Dose Alpha Lipoic Acid (ALA) Supplementation with Interval Walking Training Program after Surgical Vascular Bypass Treatment (Pilot Study)

International Journal of Kinesiology and Sports Science ◽

10.7575/aiac.ijkss.v.7n.3p.22 ◽

2019 ◽

Vol 7 (3) ◽

pp. 22

Author(s):

Sid Solakovic Solakovic ◽

Ratko Pavlovic ◽

Mensur Vrcic ◽

Emir Solakovic

Keyword(s):

Physical Activity ◽

Training Program ◽

P Value ◽

High Dose ◽

Tribulus Terrestris ◽

Graft Occlusion ◽

Amputation Level ◽

Below The Knee ◽

Significant Difference ◽

Vein Bypass

Background: Some of main raisons for the elderly graft occlusion after successful aortal-iliac, aortal Femoral and Femoral Distal Vein Bypass, progression of main disease, continuing bad life Habits and uncontrolled risk factors such are mostly: poor nutrition traditional or fast food, Smocking and Lacks of Walking and Physical Activity Habits. Objective: The primary objective of the study was to estimate influence of Interval Walking Training Program combine with Tribulus Terrestris, 3-5 gram of Taurine and high dose of 1800mg supplementation of ALA on primary potency and vascular treatment. Secondary goals of this study is determinate by establishing better understanding connection between ordinary vascular walking therapy 30-45 min and interval walking program combine with Tribulus Terrestris, Taurine and high dose of and ALA as secondary supplementation after surgical and endovascular treatment. Methodology: The study included 112 patients, at the Clinic of cardiovascular surgery, Clinical Center University of Sarajevo, age between 50 and 75 (50 patients surgical treated with aortic-iliac, aortic-femoral and femoral distal vein bypass with and without Linton-patch/Taylor patch-first group) and (62 endovascular Iliac treated patients (indication TASC II A and B) with and without support Tribulus Terrestris, high dose of ALA and Taurine - second group). Results: Final analysis has reveal the rehabilitation outcome in 83% patients with bypass above the knee was fully rehabilitated compared to 46,6% patients with bypass below the knee was statistically considered significant by using p value less than (p<0,05) In anamnestic history in 83% patient with amputation above the knee was documented the presence of hypertension, hyperlipidemia, nicotinismus and diabetes compared to 66,7% of patients with amputation level below the knee but there was no statistically significant difference (p>0,05). Conclusion: Interval Walking Training Program on Tribulus Terrestris, Taurine and high dose of ALA had a significantly and successfully higher bypass potency and rehabilitation prognosis compare to patients without supplementation and postsurgical physical therapy concept. It is obviously the is certain link between physical activity, life style modification and serum testosterone on primary bypass potency.

Download Full-text

Fast and Delayed Locomotor Response to Acute High-Dose Nicotine Administration in Adult Male Rats

Physiological Research ◽

10.33549/physiolres.932610 ◽

2013 ◽

pp. S81-S88 ◽

Cited By ~ 1

Author(s):

K. JANDOVÁ ◽

D. MAREŠOVÁ ◽

J. POKORNÝ

Keyword(s):

Adult Male ◽

Drug Application ◽

Test Session ◽

Behavioral Pattern ◽

Male Rats ◽

High Dose ◽

Delayed Response ◽

Locomotor Response ◽

Nicotine Administration ◽

Horizontal Activity

The aim of the present study was to compare the immediate and delayed locomotor response to high-dose nicotine (NIC) administration in rats. The vertical and horizontal activity of behavior in adult male rats exposed to 1 mg/kg NIC or saline (SAL) were tested in a Laboras apparatus for one hour after drug application. Animals were then returned to their cages and housed for another seven days. After this period all animals were placed in Laboras again and their behavioral pattern was retested for another period of one hour (delayed response). Horizontal activity: immediately after nicotine administration animal were less mobile (first 2-minutes interval), when compared with controls. The immobilization effect of nicotine disappeared within 4 minutes and during whole first 10-minutes interval time spent by locomotion did not differ from controls. Locomotion activity of animals treated with nicotine increased robustly in following 10 minutes and remained significantly higher in 2nd, 3rd and 5th 10-minutes interval. Vertical activity: Rearing frequency was significantly lowered by NIC administration in first two minutes of the experiment and the same was found when the duration of rearing was analyzed. Lower rearing intensity of NIC treated animals disappeared in 4 minutes and was finally higher during whole test session as compared with controls. When duration of rearing was analyzed it was significantly longer in NIC treated animals. In majority of observed behavioral aspects there were no differences between NIC treated rats and controls seven days after NIC or SAL treatment. Our results reflect effect of NIC and we conclude that NIC significantly influences behavior of experimental animals.

Download Full-text

EFFECTS OF LYCOPENE ON SPERMATOZOA MORPHOLOGY IN Balb/C MICE EXPOSED TO 2-METHOXYETHANOL (2-ME)

Folia Medica Indonesiana ◽

10.20473/fmi.v53i4.7158 ◽

2017 ◽

Vol 53 (4) ◽

pp. 264

Author(s):

Sandra Dewi Sitaresmi ◽

Reny I'tishom ◽

Arifa Mustika

Keyword(s):

Direct Contact ◽

Vas Deferens ◽

Beta Carotene ◽

Reproductive Organs ◽

The Body ◽

Control Group ◽

Morphological Examination ◽

Toxic Materials ◽

Significant Difference ◽

Better Than

The compound of 2-methoxyethanol (2-ME) is one of toxic materials that potentially damage male reproductive organs. This compound can enter the body by various means of direct contact through the skin, respiratory and digestive tract. This compound can cause oxidative stress in spermatozoa which is the main cause of spermatozoa dysfunction. Lycopene, also known as red pigment, is an antioxidant class of carotene. Lycopene has antioxidant activity twice stronger than beta carotene and ten times stronger than vitamin E. Thus, the lycopene reaction as an antioxidant in the body is better than vitamin A, C, E, and other minerals. This study was conducted to determine the effects of lycopene on spermatozoa morphology in mice exposed to 2-ME. The study was conducted on 30 mice, divided into 5 groups. The K-group was the control group without 2-methoxyethanol and lycopene, the K + group was the group with 200 mg/kg BW 2-methoxyethanol on days 1-5, and the P1, P2 and P3 groups were the ones with 200 mg/kg BW 2-methoxyethanol on days 1-5 and lycopene doses of 5, 10 and 20 mg/kg BW on days 6-35. 2-ME was given intraperito-neally and lycopene was given per sonde. On day 36, the mice were sacrificed and the epididymis and vas deferens were removed for morphological examination of spermatozoa. The observations were performed using a microscope with 1000x magnification. The results showed that there was significant difference. In the morphology of spermatozoa, the administration of lycopene increases normal morphological percentage of spermatozoa in mice exposed to 2-ME.

Download Full-text