scholarly journals Ferulic acid as major antioxidant phenolic compound of the Tetragonisca angustula honey collected in Vera Cruz - Itaparica Island, Bahia, Brazil

2024 ◽  
Vol 84 ◽  
Author(s):  
Â. C. O. Lima ◽  
E. R. Dias ◽  
I. M. A. Reis ◽  
K. O. Carneiro ◽  
A. M. Pinheiro ◽  
...  

Abstract The antioxidant activity of Tetragonisca angustula honey (TAH) and its ethanolic extract (TAEE) were investigated. The total levels of phenolic (TPC) and flavonoids (TFC) were also evaluated. The results for TPC were 19.91 ± 0.38 and 29.37 ± 1.82 mg GAE g-1 and for TFC 0.20 ± 0.02 and 0.14 ± 0.01 mg QE g-1 of TAH and TAEE, respectively. Antioxidant activities were 73.29 ± 0.49% and 93.36 ± 0.27% in the DPPH● assay and 71.73 ± 4.07% and 97.86 ± 0.35% in ABTS●+ for TAH and TAEE, respectively. The total reducing activity was determined by the method of reducing power (PR) and iron ion (Fe III) and the results varied in PR from 151.7 ± 25.7 and 230.7 ± 25.2 mg GAE L-1, for TAH and TAEE respectively and for (Fe III) in EC50 0.284 in TAEE and 0.687 in TAH. Chemical analysis by HPLC-DAD of the ethanolic extract (TAEE) revealed the presence of ferulic acid as majority phenolic component in the extract. The 1H NMR analysis confirmed this structure and showed the also presence of glucose, citric acid, succinic acid, proline and hydrocarbon derivatives. In addition, the botanical origin was also investigated and showed a multifloral characteristic, having found 19 pollen types with a botanical predominance of the Anacardiaceae family, with Tapirira pollen occurring as predominant (42.6%) and Schinus as secondary (25.7%). The results showed that T. angustula honey is an interesting source of antioxidant phenolic compounds due to its floral origin and can act as a protector of human health when consumed.

2009 ◽  
Vol 15 (5) ◽  
pp. 435-444 ◽  
Author(s):  
Zuofa Zhang ◽  
Jie Jin ◽  
Liangen Shi

The antioxidant properties and total phenolic contents of four fractions of ethanolic extract from Ramulus mori were examined. Various experimental models including superoxide radical, hydroxyl radical, 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH) scavenging activity, metal chelating activity, and reducing power were used for characterization of their antioxidant activity. The four fractions showed various degrees of efficacy in each assay in a dose-dependent manner. The third fraction with the highest amount of total phenolics was the most potent antioxidant in all assays used. In addition, the most powerful compound (oxyresveratrol) was isolated and identified followed by on-line HPLC method and characterized by different spectral analysis. Oxyresveratrol exhibited impressive antioxidant activities in scavenging the superoxide radical, hydroxide radical, and DPPH. On the basis of the results obtained, Ramulus mori may serve as a potential source of natural antioxidant due to its significant antioxidant activity and oxyresveratrol may be the most powerful antioxidant in ethanolic extracts of Ramulus mori.


2019 ◽  
Vol 18 (1) ◽  
pp. 49-60
Author(s):  
Kousik Ahmed Khan ◽  
Manik Zahan ◽  
Fatema Zohura Talukder ◽  
Riaz Uddin ◽  
Manik Chandra Shill ◽  
...  

The current investigation was carried out to evaluate the antioxidant properties of ethanolic extract of Alternanthera bicolor and to assess the potential hypoglycemic effect of the extract in alloxan-induced diabetic mice. HPLC-DAD method was used to determine polyphenolic compounds present in the extract. Different in vitro assays (i.e. DPPH radical scavenging activity test, reducing power test, NO radical inhibition assay and scavenging of hydrogen peroxide) were used to determine the antioxidant potential of the plant. Antidiabetic activity was evaluated in alloxan-induced diabetic mice by glucose tolerance test and standard biochemical analyses. HPLC-DAD analysis of the extract confirmed the presence of (+)-catechin hydrate, caffeic acid, quercetin and kaempferol. A. bicolor showed potent antioxidant activities in DPPH radical, hydrogen peroxide and nitric oxide scavenging assays. Moreover, A. bicolor showed potent reducing power and dose-dependent increment of total antioxidant capacity. Furthermore, the plant showed potent hypoglycemic activities in alloxan-induced diabetic mice. Ethanolic extract of the plant at doses of 200- and 400-mg/kg body weight (administered orally for 3 weeks) significantly decreased the elevated levels of blood glucose, lipid peroxidation product TBARS, hydroperoxides and nitric oxide in experimental animals. Apart from these activities, the ethanolic extract of the plant restored the reduced catalase function in liver. From this study, we can conclude that ethanolic extract of A. bicolor exhibited hypoglycemic and antioxidant activities in alloxan-induced diabetic mice. Dhaka Univ. J. Pharm. Sci. 18(1): 49-60, 2019 (June)


2020 ◽  
pp. 18-27
Author(s):  
Kiliobas Kiri Sha’a ◽  
Susan Artimas Pwakangdi

Antioxidants are agents which scavenge free radicals and prevent cellular damage cause by them. They reduce the damage due to free radicals by neutralizing them before they can attack the cells. The aim of this research was to evaluate the antioxidant, nutritional and antinutritional properties of two leafy vegetables, Cleome rutidosperma and Cassia tora consumed in Adamawa State, Nigeria. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) models were used to determine antioxidant activities of extracts. Nutritional composition and antinutritional properties were determined using standard procedures. There was a significant (p=0.05) decrease in the concentration of DPPH radical due to the scavenging activity of ethanol leaf extract of  Cleome rutidosperma compared to control/standard. In FRAP also, C. rutidosperma ethanolic extract exhibited higher ferric reducing power than C. tora. Proximate analysis revealed the nutrients for C. rutidosperma and C. tora as; crude protein (31.06±0.00% and 26.24±0.00%), fat (7.13±0.00% and 6.93±0.10%), ash (15.17±0.61% and 11.60±0.00%), crude fiber (11.06±0.01 and 13.19±0.10%), moisture (9.99±0.00% and 9.12±0.10%), NFE (25.60±0.10% and 32.94±0.10%) respectively. Antinutritionals in ethanolic extracts of C. rutidosperma and C. tora were; oxalates, saponins, tannins, cyanogenic glycosides, phytate and alkaloids. Both vegetables revealed good percentages of proteins which can be used to compliment other sources of protein. All antinutrients of extracts were within tolerable levels.


2021 ◽  
Vol 18 ◽  
Author(s):  
Amar Otmani ◽  
Nadia Amessis-Ouchemoukh ◽  
Zina Mouhoubi-Tafinine ◽  
Kenza Tighlit ◽  
Ibtissam Redouan ◽  
...  

Background: Honey is a sweet and flavorful natural product which comes from a lot of nectar of medicinal plants. Objective: The aims of this study were to determine the botanical origin, physicochemical parameters and the antioxidant contents and antibacterial activities of some Algerian honeys. Method: The physico-chemical parameters of the honeys analyzed were measured using the methods established by the European Honey Commission. The antioxidant contents were determined using colorimetric assays, and the antioxidant activities were estimated using reducing power, DPPH, ABTS, and FRAP assays. Antibacterial activities were measured using sensitivity testing and minimal inhibitory concentration. Results: The majority of the samples analyzed were monofloral honeys (Fabaceae) and other medicinal plant pollens were shown (Asteraceae, Tiliaceae, Myrtaceae and Apiaceae). The physicochemical parameters of the honeys were in accordance with the legislation, and the analyses of proline and HMF confirmed their authenticity. The content of total phenolic compounds and total flavonoids ranged from 26 to 159 mg GAE/100 g and 10 to 43 mg EC/100 g, respectively. The analyzed honeys showed variable antioxidant activities that differed from one honey to another, and antibacterial activity tests showed that S. aureus and K. pneumoniae were the most sensitive strains with inhibition zones of 24 to 28 mm and 8 to 35 mm in diameter, respectively. Very high correlations were observed between color, antioxidants and antioxidant activities. Conclusion: This study confirmed the powerful properties of honey to trap free radicals and to inhibit bacterial growth that could be used as a therapeutic agent.


2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Sung-Ho Lee ◽  
Seong-Wook Seo ◽  
Seung-Yub Song ◽  
Deuk-Sil Oh ◽  
Jung-Hyun Shim ◽  
...  

A method for the separation and quantification of acteoside by reverse-phase high-performance liquid chromatography (HPLC) was developed and validated. Hot water and ethanolic extracts of Osmanthus fragrans leaves and flowers were analyzed for acteoside content. Excellent linearity was obtained, with an r2 higher than 0.999. The precision, specificity, and accuracy of our method were excellent, suggesting that it can be conveniently used for the quantification of acteoside in the crude extract of O. fragrans. The hot water and ethanol extracts were analyzed, and their biological activities were tested. The extraction yields, marker (acteoside) contents, and antioxidant activities of the leaf and flower extracts were analyzed. The antioxidant activity was confirmed by measuring the 2,2-diphenyl-2-picrylhydrazyl radical scavenging activity, reducing power, and total phenolic content. The acteoside content tended to be higher in the 100% ethanol extract of O. fragrans compared to those with the other extraction conditions tested. Overall, almost all extracts prepared with ethanolic solvents tended to produce better antioxidant activity than those prepared with hot water. These results suggest that the ethanolic extract of O. fragrans could serve as a potential antioxidant and anti-inflammatory pharmaceutical source, and our validated method would be useful for the quality control of O. fragrans extracts.


2019 ◽  
Vol 35 (2) ◽  
pp. 718-722
Author(s):  
D. Roselin Jenifer ◽  
B. R. Malathy

The current investigation is focused to determine antioxidant activities of the leaf extract of Plumbago zeylanica. The leaf extracts were prepared by using multiform solvents with increasing polarity, including as hexane, chloroform, ethyl acetate and Methanol. The antioxidant activities enlisting DPPH assay, hydrogen peroxide assay, reducing power assay and nitric oxide assay method were performed for all extracts. Barring no one, the extracts had shown average to potent antioxidant activity. Uniquely, the ethyl acetate extract and the chloroform extract explicated the utmost antioxidant activity.


Author(s):  
ERNEST NOGMA SOMBIÉ ◽  
NOUFOU OUÉDRAOGO ◽  
ADAMA HILOU

Objective: This study aimed to assess the anti-inflammatory and antioxidant properties in vitro of Calotropis procera root bark. Methods: Anti-inflammatory activities of the ethanolic extract of root bark of C. procera and its fractions were evaluated on lipoxygenase and xanthine oxidase (XO). Antioxidant activity was evaluated by trapping the 2,2-diphenylpicrylhydrazyl (DPPH) radical, hydrogen peroxide (H2O2), by the reducing power ferric reducing antioxidant power. Inhibitory powers of lipid peroxidation and of degradation of D-deoxyribose as well as the contents of phenolic and triterpene compounds were also evaluated. Results: Ethanolic extract showed interesting anti-inflammatory and antioxidant activities. Butanol fraction which is one of richest fractions for flavonoids (17.38±0.13 mg EQ/10 g extracts) showed the best percentages for inhibitions of XO (60.14±4.89%), degradation of D-deoxyribose (81.04±0.77%), and the DPPH radical (71.43±6.35%). Dichloromethane fraction which is the richest in triterpenes (1067.02±5.77 mg UAE/g extracts) showed the best percentages for inhibitions of lipoxygenase (56.45±1.15%) and of lipid peroxidation (82.45±0.73%). Ethyl acetate and residual aqueous fractions, respectively, showed the best activities of inhibiting H2O2 (75.38±0.76%) and for iron-reducing capacity (23.51±1.69 mg EAA/g extracts). Conclusion: Ethanolic extract from C. procera root bark and its fractions, their particular the butanol and dichloromethane one, has anti-inflammatory and antioxidant properties which could prevent or attenuate liver injury and its complications.


Author(s):  
Gerard Hodeve Tiko ◽  
Abdou Madjid O. Amoussa ◽  
Rafiou Adamou ◽  
Adande A. Medjigbodo ◽  
Luc S. Djogbenou ◽  
...  

Aims: This study was designed to assess antiplasmodial and antioxidant activities in relation to phytochemical contents and toxicological profile of crude extracts of Cola millenii leaves. Place and Duration of Study: The study was carried out from April 2015 to November 2018 at University of Abomey-Calavi, Laboratory of Biochemistry and Bioactives Natural Products and Laboratory of Infectious Vector-borne Diseases. Methodology: Phytochemical screening of aqueous and ethanolic crude extracts was performed using standard methods. Estimation of total phenolics contains (TPC) and total flavonoids contains (TFC) was done using colorimetric methods. Antioxidant activity was assessed in vitro by 2,2-diphenyl-1-picrylhydrazyl (DPPH) Radical-Scavenging, Reducing power, superoxide radical scavenging and Hydrogen peroxide scavenging assay. Antiplasmodial activity was investigated in vitro using both field isolate and laboratory 3D7 strains of P. falciparum using Plasmodium lactate dehydrogenase (pLDH) assay. Hemoglobin release was measured spectrophotometrically to assess hemolytic power. Acute oral toxicity of extracts was evaluated on rats according to OECD 423 guideline. Results: Flavonoids, anthraquinones, coumarins, and triterpenes were recorded in both extracts while tannins were recorded only in ethanolic extract. High TPC and TFC were recorded with ethanolic extract with significate difference (P <0.01) when compared to aqueous extract. Ethanolic extract exhibited the highest parasite growth inhibition against both field isolate (41.62±1.78%) and 3D7 (45.89±1.66%) strains at 100 µg/mL. Both extracts exhibited strong antioxidant activity according to different methods used. Toxicological profile revealed no hemolytic effect on human red blood cells as well as acute toxicity signs in rats. Conclusion: This study demonstrated strong antioxidant and moderate antiplasmodial activities of C. millenii extracts without toxicity effect on rats and human erythrocytes. It would play an important role in malaria and oxidative damage control.


2020 ◽  
Vol 10 (5) ◽  
pp. 306-310
Author(s):  
Saliha Djidel ◽  
Amel Bouaziz ◽  
Assia Bentahar ◽  
Seddik Khennouf

The objective of this study was to evaluate the phenolic content, in vitro antioxidant activity and the gastroprotective effect of the ethanolic extract of pear fruits (Pyrus communis L.). The amount of polyphenols and flavonoids contents of ethanolic extract were found to be 77,50 ± 2,50 mg GAE/g of dry extract and 0,31 ± 0,003 mg QE/ g of dry extract, respectively. Whereas the amount of tannins was 61,43 ± 0,009 mg TAE/ g of dry extract. Hydroxyl radical scavenging and reducing power tests were used to evaluate the antioxidant activities of this extract. The scavenging effect of ethanolic extract against hydroxyl radicals showed IC50 value of 2.71 ± 0.02 mg/ ml. Reducing power test indicated the ability of ethanolic extract of pear fruits to reduce Fe+2 to Fe+3  with EC50value of 1.043±0.01 mg/ml. The treatment of rats with the ethanolic extract at  dose of 200 mg/kg reduced the ulcerogenic effect of ethanol with value of protection rate of 94.44%, but the protection rate decreased in 600 mg/kg treated group  to 90.17%.  In conclusion, natural antioxidants derived from pear fruits can prevent gastric ulcer through their antioxidant activity  Keywords: Pyrus communis L., Polyphenols, Antioxidant activity, Ulcer, Rat.


2019 ◽  
Vol 37 (No. 5) ◽  
pp. 351-358 ◽  
Author(s):  
Nebojša Živić ◽  
Slaviša Milošević ◽  
Vidoslav Dekić ◽  
Biljana Dekić ◽  
Novica Ristić ◽  
...  

The content of phytochemicals, total phenolics, total flavonoids and antioxidant potential of extracts of Juniperus communis L. and Juniperus oxycedrus L. berries were determined. Ethanol, ethyl acetate and chloroform were used for the process of extraction. Phytochemical monitoring was based on already known methods, while in vitro antioxidant activities were done by DPPH assay. Phytochemical screening showed a wide spectrum of phytochemicals. Ethanolic extract of Juniperus communis L. possesses the strongest antioxidant activity (IC50 = 28.55 ± 0.24 µ/ml), as well the higher contents of total phenolics, 189.82 ± 0.27 mg of gallic acid equivalent per g of dried weight extract (mg GAE/g extract DW), and total flavonoids, 42.85 ± 0.13 mg of rutin equivalents per g of dried weight extract (mg RE/g extract DW). The results indicated the potential application of the tested extracts as significant antioxidants.


Sign in / Sign up

Export Citation Format

Share Document