CELLS CULTURED FROM THE GROWING TIP OF RED DEER ANTLER EXPRESS ALKALINE PHOSPHATASE AND PROLIFERATE IN RESPONSE TO INSULIN-LIKE GROWTH FACTOR-I

1994 ◽  
Vol 143 (2) ◽  
pp. R9-R16 ◽  
Author(s):  
Joanna S. Price ◽  
Babatunde O. Oyajobi ◽  
Richard O. C. Oreffo ◽  
R. Graham G. Russell
Keyword(s):  
Alkaline Phosphatase ◽  
Growth Factor ◽  
Red Deer ◽  
Cultured Cells ◽  
Cell Protein ◽  
Insulin Like Growth Factor ◽  
Deer Antler ◽  
Alp Activity ◽  
Antler Growth ◽  
Cells Cultured

ABSTRACT Deer antler growth provides a unique natural model of rapid and complete bone regeneration. In this study, the distal antler tips of male red deer (Cervus elaphus) were collected post-mortem during the annual growth period (April-August), and an in vitro system established for the culture of cells from three regions; the inner layer of the perichondrium, the reserve mesenchyme and the cartilage zone. Alkaline phosphatase (ALP) expression by cultured cells, as demonstrated by enzyme histochemistry and biochemical assay, reflected the stage of cellular differentiation. ALP activity was highest in cells cultured from the hypertrophic cartilage region (3.6 ± 0.2 μmol/μg cell protein/minute), and lowest in undifferentiated mesenchymal cells (0.3 ± 0.01 μmol/μg cell protein/minute). ALP expression was lost with passage in culture. Levels of ALP activity in cultured cells correlated with the pattern and extent of enzyme expression in tissue sections as demonstrated by histochemical staining. Insulin-like growth factor (IGF)-I (10−9M-10−7M) was found to be mitogenic for cultured cells from all three zones as shown by increased incorporation of [3H]thymidine into DNA. These results demonstrate that cells from three different regions of the antler tip can be maintained in culture, and that antler cells share certain phenotypic characteristics of growth plate chondrocytes. These data provide further evidence of a role for IGF-1 in the regulation of antler growth. Antler regrowth is a potentially useful model for the study of the factors that regulate bone formation.

Expression and localization of insulin-like growth factor-I in four parts of the red deer antler

2007 ◽  
Vol 25 (4) ◽  
pp. 264-279 ◽  
Author(s):  
Lijuan Gu ◽  
Eunkyoung Mo ◽  
Zhihong Yang ◽  
Xuemei Zhu ◽  
Zheming Fang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Red Deer ◽  
Insulin Like Growth Factor ◽  
Deer Antler ◽  
Factor I ◽  
Growth Factor I

scholarly journals Partial Purification and Quantification of Insulin-like Growth Factor-I from Red Deer Antler

2007 ◽  
Vol 17 (10) ◽  
pp. 1321-1329 ◽  
Author(s):  
Lijuan Gu ◽  
Eun-Kyoung Mo ◽  
ZheMing Fang ◽  
BaiShen Sun ◽  
XueMei Zhu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Red Deer ◽  
Partial Purification ◽  
Insulin Like Growth Factor ◽  
Deer Antler ◽  
Factor I ◽  
Growth Factor I

Insulin-like growth factor 1, growth and body composition in red deer stags

1991 ◽  
Vol 53 (2) ◽  
pp. 237-242 ◽  
Author(s):  
J. M. Suttie ◽  
I. D. Corson ◽  
P. D. Gluckman ◽  
P. F. Fennessy
Keyword(s):  
Growth Rate ◽  
Weight Gain ◽  
Growth Factor ◽  
Red Deer ◽  
Live Weight ◽  
Late Summer ◽  
Carcass Weight ◽  
Insulin Like Growth Factor ◽  
Live Weight Gain ◽  
Antler Growth

AbstractInsulin-like growth factor 1 (IGF1) correlates with antler growth and body growth in penned red deer stags. The present study aimed to investigate the relationships among plasma concentration of IGF1, body growth and carcass composition in grazing stags. Twenty-eight 10-month-old stags were kept at pasture from September to February. They were bled, weighed and their antlers measured fortnightly. Plasma was analysed for total IGF1. All stags were slaughtered in February (aged 15 months); organ weights were recorded and the carcasses were analysed chemically for water, fat and ash. The stags began the study weighing 60·7 (s.e. 1·4) kg and were 96·3 (s.e. 2·0) kg at the end. They grew rapidly in spring and early summer but growth rate slowed in mid summer before increasing again in late summer.Total plasma 1GF1 for each stag throughout the study correlated positively with antler length (P < 0·001), total live-weight gain (P < 0·001), hot carcass weight (P < 0·01), fat-free carcass weight (P < 0·01), carcass fat weight (P > 0·05) and carcass fat percentage (P > 0·05). Thus IGF1 correlated significantly with all measures of body weight except fat. IGF1 correlated positively with antler growth rate (P < 0·001) calculated individually for each stag during each fortnight. IGF1 correlated positively with the spring phase of live-weight gain calculated as above (P < 0·001) but negatively (P < 0·01) with live-weight gain during the late summer growth phase. The relationships between IGF1 and growth in penned stags also pertain in grazing animals, and in addition 1GF1 is closely associated with carcass lean mainly via an overall effect on body size.

Pulsatile growth hormone, insulin-like growth factors and antler development in red deer (Cervus elaphus scoticus) stags

1989 ◽  
Vol 121 (2) ◽  
pp. 351-360 ◽  
Author(s):  
J. M. Suttie ◽  
P. F. Fennessy ◽  
I. D. Corson ◽  
F. J. Laas ◽  
S. F. Crosbie ◽  
...  
Keyword(s):  
Growth Rate ◽  
Growth Factor ◽  
Red Deer ◽  
Pulse Amplitude ◽  
Pulse Frequency ◽  
Insulin Like Growth Factor ◽  
Antler Growth ◽  
Two Samples ◽  
Igf I ◽  
Antler Development

ABSTRACT Plasma samples taken every 30 min over a 26-h period each month from six 4- to 15-month-old red deer stags were analysed for GH. In addition, two samples taken at 10.00 and 22.00 h were analysed for insulin-like growth factor-I (IGF-I) and insulin-like growth factor-II (IGF-II). A concentrate diet was available ad libitum. Food intake, body weight and antler status were recorded. Concentrations of GH were analysed using the PULSAR peak detection routine. Secretion of GH was pulsatile in every month of sampling, but the pattern of pulsatility differed seasonally. During the autumn and early winter (April–June in the Southern hemisphere) GH pulses were frequent and of low amplitude. In contrast, GH pulses in spring (August–September) were of high amplitude and high frequency resulting in a high mean level of GH circulating in the plasma. In early summer (November) the GH pulse amplitude was much lower and pulse frequency fell. There was a rise in GH pulse frequency not accompanied by an increase in GH pulse amplitude in summer (December–January). GH pulse amplitude seemed to be the main determinant of mean GH plasma level. Secretion of IGF-I was raised 1 month after peak monthly mean GH secretion. There was little consistent relationship between concentrations of IGF-II and mean daily GH. Concentrations of GH correlated positively and significantly with liveweight gain and antler growth rate with a delay of 1 month. Significantly positive correlations between concentrations of IGF-I, liveweight gain and antler growth rate were observed. It is considered that the spring and summer (September–December) seasonal acceleration of liveweight gain and antler development in stags could be a consequence of high winter/early spring (August–September) GH pulse frequency and amplitude resulting in increased concentrations of IGF-I, particularly in October. Journal of Endocrinology (1989) 121, 351–360

The rôles of photoperiod and nutrition in the seasonal increases in growth and insulin-like growth factor-1 secretion in male red deer

2001 ◽  
Vol 73 (2) ◽  
pp. 305-311 ◽  
Author(s):  
J. R. Webster ◽  
I. D. Corson ◽  
R. P. Littlejohn ◽  
S. K. Martin ◽  
J. M. Suttie
Keyword(s):  
Growth Factor ◽  
Food Intake ◽  
Growth Rates ◽  
Energy Demand ◽  
Red Deer ◽  
Young Male ◽  
Live Weight ◽  
Metabolizable Energy ◽  
Plasma Prolactin ◽  
Insulin Like Growth Factor

AbstractYoung male red deer follow a seasonal growth pattern that can be shifted by altering the photoperiod they experience. An increase in photoperiod to 16 h of light per day (16L : 8D) during winter advances the onset of rapid growth and high food intake that normally commences in spring. These changes are associated with increased growth hormone (GH) and insulin-like growth factor-1 (IGF-1) secretion. The GH/IGF-1 axis is acutely sensitive to the level of nutrition and the relative rôles of photoperiod and nutrition in determining the spring IGF-1 rise is unknown. The present experiment set out to examine this by exposing two groups of deer (no. = 8 per group) to a photoperiod shift during their 1st year of life (16L : 8D from 2 June), designed to cause accelerated growth and increased food intake after approximately 7 weeks. However, after 6 weeks the food intake (pellets containing 11 MJ metabolizable energy and 160 g crude protein per kg dry matter (DM)) of one group (LDRES) was clamped, thereby preventing the intake component of the response. The intake of the other group (LDAL) remained ad libitum for a further 12 weeks until 6 October, when the experiment concluded.During the first 6 weeks of 16L : 8D, growth rate (118 (s.e. 15·4) g/day) and food intake (1·37 (s.e. 0·031) kg DM per head per day) did not differ between the groups. Food intake following the clamp in LDRES averaged 1·40 (s.e. 0·015) kg per head per day. The intake of LDAL increased 2 weeks after the clamp and thereafter was higher than LDRES (P < 0·001). Food intake of LDAL averaged 2·13 (s.e. 0·051) kg during the nutritional clamp period. Growth rates increased in both groups during the first 3 weeks of the clamp, averaging 237 (s.e. 25·0) g/day, then growth slowed in LDRES and live weights diverged. Growth rates until the end of the experiment (147 (s.e.23·0) g/ day v. 299 (s.e. 12·5) g/day, P < 0·001) and mean live weight over the last 5 weeks of the experiment were lower (P < 0·05) in LDRES than LDAL, weights reaching 88·3 (s.e. 1·86) kg and 97·9 (s.e. 2·74) kg respectively on the final sampling date. Metatarsal bone length grew more in LDAL than in LDRES (3·1 v. 2·2 cm, s.e.d. = 0·23, P < 0·01). Prior to the nutritional clamp, mean plasma prolactin and IGF-1 concentrations increased at 3 and 6 weeks after 16L : 8D respectively, in both groups. Prolactin concentrations were lower in LDRES than LDAL on two occasions, at weeks 3 and 7 after the onset of the nutritional clamp, and IGF-1 concentrations were lower in LDRES than LDAL (676 v. 872 ng/ml, s.e.d. = 73·8, P < 0·05) over the last 7 weeks of sampling.In summary, a photoperiodically driven increase in IGF-1 occurred even when the usual associated increase in food intake was prevented. This indicates that the seasonal IGF-1 rise in red deer is not a consequence of the increased food intake, although the latter appears necessary to maintain elevated IGF-1 concentrations. The rise in IGF-1 may therefore be considered as a component of the photoperiodically entrained seasonal drive to grow, and the increase in food intake a response to satisfy the increased energy demand.

Alkaline Phosphatase, Sucrase, and Insulin-like Growth Factor Receptors are Present in Atrophied Small Bowel 14 Years after Jejunoileal Bypass

1993 ◽  
Vol 3 (3) ◽  
pp. 319-323
Author(s):  
Ruth S. MacDonald ◽  
William H. Thornton Jr ◽  
Boyd E. Terry ◽  
Barbro A.-L. Barrett ◽  
Edward H. Adelstein
Keyword(s):  
Alkaline Phosphatase ◽  
Growth Factor ◽  
Small Bowel ◽  
Growth Factor Receptors ◽  
Jejunoileal Bypass ◽  
Insulin Like Growth Factor

Characteristics of first-antler growth in reindeer and their association with seasonal fluctuations in steroid and insulin-like growth factor 1 levels

1998 ◽  
Vol 76 (11) ◽  
pp. 2096-2102 ◽  
Author(s):  
J E Blake ◽  
J E Rowell ◽  
J M Suttie
Keyword(s):  
Growth Factor ◽  
Growth And Development ◽  
Rangifer Tarandus ◽  
Plasma Testosterone ◽  
Insulin Like Growth Factor ◽  
Seasonal Fluctuations ◽  
Testosterone Levels ◽  
Rangifer Tarandus Tarandus ◽  
Antler Growth ◽  
Measurable Growth

Growth and development of the pedicles and first antlers were monitored in seven reindeer (Rangifer tarandus tarandus) calves (three males, four females) from birth to 1 year of age. Calves were weighed, blood was sampled, and antler observations and (or) measurements were taken on the day of birth and days 2, 4, and 7 and then at weekly intervals for the remainder of the year. Prior to measurable growth, the frontal lateral crest was carefully palpated for evidence of pedicle initiation, starting on the day of birth. Calves were born between April 8 and 26, 1994. Pedicle initiation occurred in all reindeer by day 7. Velvet skin first appeared when calves were 4-6 weeks old, when estimated pedicle height was 3.5 ± 0.9 cm (mean ± SD). Antler growth in both sexes followed a sigmoidal pattern, producing a mean antler length of 33.5 ± 4.2 and 46.7 ± 13.8 cm in females and males, respectively. Antler growth was complete by week 18 in females and week 21 in males. Velvet cleaning was associated with a significant increase in plasma testosterone levels in males and estradiol-17beta (but not testosterone) levels in females. Insulin-like growth factor 1 levels were significantly higher in males than in females and were positively correlated with the period of most rapid antler growth in both sexes. Casting occurred between March 14 and April 17, when steroid levels were at a minimum, and was immediately followed by growth of the second antler.

Colocalization of insulin-like growth factor-binding protein with insulin-like growth factor I

1991 ◽  
Vol 261 (1) ◽  
pp. F22-F28 ◽  
Author(s):  
S. Kobayashi ◽  
D. R. Clemmons ◽  
M. A. Venkatachalam
Keyword(s):  
Growth Factor ◽  
Cultured Cells ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Intense Staining ◽  
Factor Binding ◽  
Factor I ◽  
Collecting Ducts ◽  
Igf I ◽  
Growth Factor I

We report the localization of insulin-like growth factor I (IGF-I) and a 25-kDa form of insulin-like growth factor-binding protein (IGF-BP-1) in adult rat kidney. The antigens were localized using a rabbit anti-human IGF-I antibody, and a rabbit anti-human IGF-BP-1 antibody raised against human 25-kDa IGF-BP-1 purified from amniotic fluid. Immunohistochemistry by the avidin-biotin peroxidase conjugate technique showed that both peptides are located in the same nephron segments, in the same cell types. The most intense staining was in papillary collecting ducts. There was moderate staining also in cortical collecting ducts and medullary thick ascending limbs of Henle's loop. In collecting ducts the antigens were shown to be present in principal cells but not in intercalated cells. In distal convoluted tubules, cortical thick ascending limbs, and in structures presumptively identified as thin limbs of Henle's loops there was only modest staining. The macula densa, however, lacked immunoreactivity. Colocalization of IGF-I and IGF-BP-1 in the same cells supports the notion, derived from studies on cultured cells, that the actions of IGF-I may be modified by IGF-BPs that are present in the same location.

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