Formulation of Nanoemulgel from Extracts of Musa acuminata: In-Vitro Kinetics and Antimicrobial Studies

2021 ◽  
pp. 1-8
Author(s):  
Gaanapriya Veeramani ◽  
Sivakumar Vaiyazhipalayam Murugaiyan ◽  
Thirumarimurugan Marimuthu
Keyword(s):  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Biological Activities ◽  
Musa Acuminata ◽  
Alpha Tocopherol ◽  
Malassezia Furfur ◽  
Antimicrobial Studies ◽  
Ph Range ◽  
In Vitro Kinetics

The study was intended to formulate nanoemulgel from the leaf extract of Musa acuminata. Ethanol and Chloroform were used as solvents in the ratio of 1:8 (%w/v). DL- alpha-Tocopherol, characterized by GCMS, was identified to be the major component with potential biological activities. Based on a pseudo ternary plot, the 1:1 Smix (Surfactant: Co- Surfactant) ratio was optimized as it posed maximum regions of emulsion. The prepared nanoemulgel was evaluated for physical appearance, pH, spreadability, and swelling index. The appearance was pale yellowish-white, translucent within a pH range of 5-5.8. Antimicrobial studies were performed against dandruff-causing microbes (Staphylococcus epidermidis and Malassezia furfur). Invitro studies were carried out for optimized formulations of EG2, EG4, CG2, and CG3. The drug release of 94.28% after 12 h with Higuchi plot of R2 value as 0.99 was observed for EG2. The kinetically optimized formulation, EG2 was found to have good spreadability of 12.2 (g cm) s−1 and a swelling index of 64%.

scholarly journals BIOLOGICAL ACTIVITIES OF NOVEL IN VITRO RAISED STEVIA PLANT

2017 ◽  
Vol 10 (8) ◽  
pp. 240 ◽  
Author(s):  
Neeta Raj Sharma ◽  
Vineet Meshram ◽  
Mahiti Gupta
Keyword(s):  
Pancreatic Lipase ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Activity ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Gram Negative Bacteria ◽  
Herbal Formulation ◽  
Further Development

  Objective: This communication explores a lead fraction from methanolic extract of novel Stevia species raised under in vitro conditions for its various biological activities.Methods: The dried Stevia leaves were crushed in methanol to get the polar extract. This methanol extract was tested for pancreatic lipase and alpha-amylase inhibitory activity using quantitative plate assays. Antibacterial property of the extract was also evaluated against Staphylococcus epidermidis, Escherichia coli, Bacillus subtilis, Staphylococcus aureus, and Pseudomonas aeruginosa. Further, the antioxidant potential was evaluated using 1,1-diphenyl-2-picrylhydrazyl.Results: The methanolic extract inhibited pancreatic lipase with IC50 of 5.74 μg/ml in a similar manner to a well-known anti-obesity drug in the market orlistat. The methanolic extract also showed a better pancreatic α-amylase inhibitory activity (IC50=88 μg/ml) than acarbose. Further, the lead fraction exhibited 88.48% antioxidant activity. It also exhibited broad spectrum antimicrobial activity against the spectrum of Gram-positive and Gram-negative bacteria tested under laboratory conditions with a minimal inhibitory concentration ranging from 1.95 to 31.25 μg/ml.Conclusion: Thus, this study signifies the vast potential of the lead fraction from a novel Stevia species for further development into a herbal formulation for prevention of various infectious and non-infectious diseases.

In-vitro Investigation of Wound Healing Potential of Musa acuminata Leaf Extract

2021 ◽  
Vol 11 (3) ◽  
pp. 437-449
Author(s):  
Ghosh Tanmoy ◽  
Rajamanickam Deveswaran ◽  
Sinhari Apurba ◽  
K. Kavana ◽  
R. Monisha ◽  
...  
Keyword(s):  
Wound Healing ◽  
Leaf Extract ◽  
Musa Acuminata ◽  
In Vitro Investigation

scholarly journals Green Synthesis of Reduced Graphene Nanosheets Using Leaf Extract of Tridax procumbens and its Potential In-Vitro Biological Activities

2020 ◽  
Vol 2 (1) ◽  
pp. 93
Keyword(s):  
Graphene Oxide ◽  
Green Synthesis ◽  
Reduced Graphene Oxide ◽  
Leaf Extract ◽  
Spectroscopic Analysis ◽  
Graphene Nanosheets ◽  
Biological Activities ◽  
Tridax Procumbens ◽  
Reduced Graphene

Graphene oxide nanoparticles has found immense application in bio therapeutics owing to its biocompatibility and enhanced effectiveness in drug delivery. The present study investigates the green synthesis of reduced graphene oxide using leaf extract using Tridax procumbens and testing its in-vitro biological activities. The biosynthesized reduced graphene oxide (rGO) was characterized by various spectroscopic and microscopic techniques. UV-vis spectroscopic analysis primarily detected the shift in the absorption peak from 232 to 287 nm confirming the reduction of TP-rGO. FTIR spectra of TP-rGO confirmed effective deoxygenation of GO assisted by bioactive molecules present in the leaf extract. Raman spectroscopic analysis identified successful reduction of GO through the presence of D band at 1329 cm-1 and G band at 1577 cm-1. XRD spectra of TP-rGO showed diffraction peak 2θ at 25o confirming the presence of reduced GO. SEM analysis revealed the presence of graphene nanosheets appearing as large aggregated structure. The bioreduced rGO showed anti-oxidant effect against DPPH radical observed in concentration dependent manner. The TP-rGO nanosheets exhibited bactericidal effect against gram positive bacteria: B.subitils (18 mm) and S. epidermis (9 mm) and gram negative bacteria: E.coli (14 mm) and P. aeruginosa (6 mm) at 100 µg/ml confirming the enhanced anti-bacterial effects of TP-rGO against gram positive pathogens. Hence, it is highlighted that leaf extract of Tridax procumbens act as green reducing agent for the successful biosynthesis of rGO nanosheets demonstrating potential in-vitro biological activities and has great scope in the preparation of nano drugs for the treatment of various diseases.

scholarly journals Theoretical Molecular Properties Prediction, Docking and Antimicrobial Studies on Anisidine-Isatin Schiff Bases

2021 ◽  
Vol 33 (12) ◽  
pp. 3025-3030
Author(s):  
G.K. Ayyadurai ◽  
R. Jayaprakash ◽  
S. Rathika
Keyword(s):  
Staphylococcus Aureus ◽  
Schiff Bases ◽  
Staphylococcus Epidermidis ◽  
Biological Activities ◽  
Position Effect ◽  
Group Position ◽  
Antimicrobial Studies ◽  
Main Protease ◽  
Lactam Ring ◽  
Molecular Properties Prediction

The continuous intake of a specific antibiotic for diseases is continuously reducing the immunity of the human in course of time, which includes Covid-19 treatment. Recent research on Schiff bases shows the promising biological activities and good antibacterial results. In this study, three Schiff bases with lactam ring using isatin and three different anisidines in presence of acetic acid were synthesized and characterized. Drug likeness was examined using Molsoft and docking against the target proteins such as 5J6R, 3L9L, 5HVY, covid main protease and 3ZBO proteins for drug suitability. The experimental antibacterial activity against Gram-positive strains like Staphylococcus aureus, Bacillus subtilis and Staphylococcus epidermidis. Among the synthesized compounds, three Schiff bases ortho and meta substituted compounds exhibited good results when compared to para compound, where the methoxyl group position effect was observed.

scholarly journals Effects of Ulva sp. Extracts on the Growth, Biofilm Production, and Virulence of Skin Bacteria Microbiota: Staphylococcus aureus, Staphylococcus epidermidis, and Cutibacterium acnes Strains

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4763
Author(s):  
Mathilde Fournière ◽  
Gilles Bedoux ◽  
Djouhar Souak ◽  
Nathalie Bourgougnon ◽  
Marc G. J. Feuilloley ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Biological Activities ◽  
Opportunistic Pathogen ◽  
Bacterial Biofilm ◽  
Skin Bacteria ◽  
Assisted Extraction ◽  
Cutibacterium Acnes ◽  
First Time

Ulva sp. is known to be a source of bioactive compounds such as ulvans, but to date, their biological activity on skin commensal and/or opportunistic pathogen bacteria has not been re­ported. In this study, the effects of poly- and oligosaccharide fractions produced by enzyme-assisted extraction and depolymerization were investigated, for the first time in vitro, on cutaneous bacteria: Staphylococcus aureus, Staphylococcus epidermidis, and Cutibacterium acnes. At 1000 μg/mL, poly- and oligosaccharide fractions did not affect the growth of the bacteria regarding their generation time. Polysaccharide Ulva sp. fractions at 1000 μg/mL did not alter the bacterial biofilm formation, while oligosaccharide fractions modified S. epidermidis and C. acnes biofilm structures. None of the fractions at 1000 μg/mL significantly modified the cytotoxic potential of S. epidermidis and S. aureus towards keratinocytes. However, poly- and oligosaccharide fractions at 1000 μg/mL induced a decrease in the inflammatory potential of both acneic and non-acneic C. acnes strains on keratinocytes of up to 39.8%; the strongest and most significant effect occurred when the bacteria were grown in the presence of polysaccharide fractions. Our research shows that poly- and oligosaccharide Ulva sp. fractions present notable biological activities on cutaneous bacteria, especially towards C. acnes acneic and non-acneic strains, which supports their potential use for dermo-cosmetic applications.

scholarly journals Green Synthesis of Reduced Graphene Nanosheets using Leaf Extract of Tridax procumbens and its Potential In Vitro Biological Activities

2020 ◽  
Vol 11 (3) ◽  
pp. 9975-9984
Keyword(s):  
Graphene Oxide ◽  
Green Synthesis ◽  
Leaf Extract ◽  
Graphene Nanosheets ◽  
Biological Activities ◽  
Bioactive Molecules ◽  
Dependent Manner ◽  
Tridax Procumbens ◽  
Reduced Graphene

Graphene oxide nanoparticles have found immense application in biotherapeutics owing to its biocompatibility and enhanced effectiveness in drug delivery. The present study investigates the green synthesis of reduced graphene oxide (rGO) using leaf extract using Tridax procumbens and testing its in-vitro biological activities. The biosynthesized TP-rGO was characterized by various spectroscopic and microscopic techniques. UV-vis spectroscopic primarily detected the absorption peak from 232 to 287 nm. XRD spectra showed diffraction peak 2θ at 25o, confirming the presence of reduced GO. FTIR and Raman spectra confirmed effective deoxygenation of GO assisted by bioactive molecules present in the leaf extract. EDAX revealed the presence of carbon (74%) and oxygen (24%). TEM analysis revealed the presence of graphene nanosheets structure appearing as a thin sheet stacked with one another. The bioreduced rGO showed an antioxidant effect against DPPH radical observed in a concentration-dependent manner. The TP-rGO nanosheets were confirming the enhanced antibacterial effects against gram-positive pathogens than gram-negative bacteria. Hence, it is highlighted that leaf extract of T. procumbens act as a green, reducing agent for the successful biosynthesis of rGO nanosheets demonstrating potential in-vitro biological activities and has great scope in the preparation of nano drugs for the treatment of various diseases.

scholarly journals Phytochemical Screening and Antibacterial Test of Leaf Extract of Canar Susu (Smilax macrocarpa Blume) Against Eschercihia coli, Pseudomonas aeruginosa, and Staphylococcus epidermidis

2019 ◽  
Vol 7 (1) ◽  
pp. 28
Author(s):  
Lela Lailatul Khumaisah ◽  
Vina Juliana Anggraeni ◽  
Muhamad Salman Fareza
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Biological Activities ◽  
Ash Content ◽  
Phytochemical Screening ◽  
E Coli ◽  
Activity Test ◽  
Preliminary Study

Smilax is one of the genus Smilacaceae is widely used as a medicinal plant because it contains various secondary metabolites with some bioactivity, such as anti-inflammatory, antirheumatic, analgesic, antioxidant, anticancer and antibacterial. One species of Smilax that has not been studied and only grew in Indonesia is Smilax macrocarpa Blume (canar susu). Therefore, a preliminary study of phytochemicals and biological activities is required to encourage progress and novelty in science and to know its phylogenetics in Indonesia's biodiversity. The research was done by extraction method using maceration with methanol as a solvent. Simplicia characteristic, toxicity test with BSLT method, phytochemical screening according to Harborne method, and antibacterial activity test using microdilution against including Escherihia coli, Pseudomonas aeruginosa, and Staphylococcus epidermidis were performed to leaf extract of canar susu. The results obtained that methanol extract of canar susu leaves contains alkaloids, flavonoids, steroids, tannins, terpenoids, saponins, and glycosides. Water content, ash content, acid-soluble ash content, water sari content, and alcohol sari concentration 8.74%; 3.60%; 0.11%; 19.01% and 5.40% respectively. Toxicity results obtained LC50 680.07 ppm. Antibacterial activity test against E. coli has MIC 625 ppm, whereas in P. aeruginosa and S. epidermidis ATCC 12228 are 1.250 ppm. The MBC values for E. coli, P. aeruginosa, and S. epidermidis ATCC 12228 were 5,000 ppm. Based on this result known S. macrocarpa Blume is not potential as antibacterial, but potential as biopesticide according to toxicity result. 

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