Cyanobacteria Total Lipid Extraction from Polycarbonate Filters v1

2021 ◽  
Author(s):  
Robbie Martin ◽  
Steven W Wilhelm ◽  
Katarina A. Jones ◽  
Hector Castro ◽  
Shawn Campagna
Keyword(s):  
Mass Spectrometry ◽  
Total Lipid ◽  
Lipid Analysis ◽  
Lipid Extraction ◽  
Total Lipid Extraction ◽  
Additional Information ◽  
Field Samples ◽  
Cellular Lipids ◽  
Yeast Lipid

This protocol is designed/used for extraction of total cellular lipids from cyanobacteria samples (either lab cultures or field samples) collected on polycarbonate filters for use in lipid analysis and quantification via mass spectrometry. Please contact Dr. Steven Wilhelm ([email protected]) or Robbie M. Martin ([email protected]) for additional information regarding this protocol. Modified from Guan, X. L., Riezman, I., Wenk, M. R., & Riezman, H. (2010). Yeast lipid analysis and quantification by mass spectrometry. Methods in Enzymology, 470, 369-391.

scholarly journals Rapid Quantification of Yeast Lipid using Microwave-Assisted Total Lipid Extraction and HPLC-CAD

2013 ◽  
Vol 85 (10) ◽  
pp. 4912-4919 ◽  
Author(s):  
Sakda Khoomrung ◽  
Pramote Chumnanpuen ◽  
Suwanee Jansa-Ard ◽  
Marcus Ståhlman ◽  
Intawat Nookaew ◽  
...  
Keyword(s):  
Total Lipid ◽  
Lipid Extraction ◽  
Total Lipid Extraction ◽  
Microwave Assisted ◽  
Rapid Quantification ◽  
Yeast Lipid

scholarly journals The BUME method: a novel automated chloroform-free 96-well total lipid extraction method for blood plasma

2012 ◽  
Vol 53 (8) ◽  
pp. 1690-1700 ◽  
Author(s):  
Lars Löfgren ◽  
Marcus Ståhlman ◽  
Gun-Britt Forsberg ◽  
Sinikka Saarinen ◽  
Ralf Nilsson ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Total Lipid ◽  
Extraction Method ◽  
Lipid Extraction ◽  
Total Lipid Extraction

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

1959 ◽  
Vol 37 (1) ◽  
pp. 911-917 ◽  
Author(s):  
E. G. Bligh ◽  
W. J. Dyer
Keyword(s):  
Rapid Method ◽  
Total Lipid ◽  
Lipid Extraction ◽  
Fish Muscle ◽  
Lipid Extract ◽  
Total Lipid Extraction ◽  
Extraction And Purification ◽  
Chloroform Layer ◽  
Frozen Fish ◽  
Entire Procedure

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

scholarly journals Comparison between several methods of total lipid extraction from Chlorella vulgaris biomass

2015 ◽  
Vol 22 ◽  
pp. 95-99 ◽  
Author(s):  
Raquel Rezende dos Santos ◽  
Daniel Mendonça Moreira ◽  
Claudete Norie Kunigami ◽  
Donato Alexandre Gomes Aranda ◽  
Cláudia Maria Luz Lapa Teixeira
Keyword(s):  
Total Lipid ◽  
Chlorella Vulgaris ◽  
Lipid Extraction ◽  
Total Lipid Extraction

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

1959 ◽  
Vol 37 (8) ◽  
pp. 911-917 ◽  
Author(s):  
E. G. Bligh ◽  
W. J. Dyer
Keyword(s):  
Rapid Method ◽  
Total Lipid ◽  
Lipid Extraction ◽  
Fish Muscle ◽  
Lipid Extract ◽  
Total Lipid Extraction ◽  
Extraction And Purification ◽  
Chloroform Layer ◽  
Frozen Fish ◽  
Entire Procedure

Lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials. The entire procedure can be carried out in approximately 10 minutes; it is efficient, reproducible, and free from deleterious manipulations. The wet tissue is homogenized with a mixture of chloroform and methanol in such proportions that a miscible system is formed with the water in the tissue. Dilution with chloroform and water separates the homogenate into two layers, the chloroform layer containing all the lipids and the methanolic layer containing all the non-lipids. A purified lipid extract is obtained merely by isolating the chloroform layer. The method has been applied to fish muscle and may easily be adapted to use with other tissues.

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