scholarly journals Expression Changes of Genes Related to Germination Based on EST Database under Priming Treatment by Gibberellic Acid in Perilla frutescens (Korean Perilla)

2021 ◽  
Vol 26 (02) ◽  
pp. 251-256
Author(s):  
Eun Soo Seong
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Plant Development ◽  
Expressed Sequence Tag ◽  
Binding Protein ◽  
Germination Rate ◽  
Seed Priming ◽  
Perilla Frutescens ◽  
Magnesium Chelatase ◽  
Protochlorophyllide Reductase

It is very important to establish an optimal seed priming process in order to increase the vitality of the seeds and promote the metabolism for the germination of the seeds. The optimum concentrations and species of priming agents to improve seed germination of both medicinal plants were also estimated. To improve the germination rate of Perilla frutescens(Korean perilla) seeds, various seed priming agents were used to analyze seed germination rates in the Saeyeopsil, Okdong and 141 collection Korean perilla cultivars. The agents used for seed priming were CaCl2, Ca(NO3)2, NaCl, K3PO4, polyethylene glycol, and gibberellic acid (GA3). When 0.1 mMGA3was used for seed priming, germination rates of Okdong, and the 141 collection showed a greater than 70% increase compared to the controls. Nine genes were selected for expression analysis by searching for genes related to seed germination and plant development in the EST(Expressed Sequence Tag) database of the Korean perilla cDNA library. GA3priming treatment for 1 d induced higher transcriptional levels of genes related to germination and plant developmentthan controls treated with water only. These genes were identified as protochlorophyllide reductase-like, magnesium-chelatase subunit ChlI, heme-binding protein 2-like, glyceraldehyde 3-phosphate dehydrogenase A, Chlorophyll a-b binding protein 6, B2 protein, 2-Cys peroxiredoxin BAS1, and 21 kDa protein. From these results, we suggest that when priming Korean perilla seeds with GA3, a large number of genes involved in plant development at early stages of seed germination play a role in improving the seed germination rate. Also, these induced genes are ideal candidate biomarkers for seed priming of Korean perilla. Specially, protochlorophyllide reductase-like is thought to be a potential gene for future molecular marker.© 2021 Friends Science Publishers

scholarly journals Accelerating Seed Germination and Juvenile Growth of Sorghum (Sorghum bicolor L. Moench) to Manage Climate Variability through Hydro-Priming

Atmosphere ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 419
Author(s):  
Siaka Dembélé ◽  
Robert B. Zougmoré ◽  
Adama Coulibaly ◽  
John P. A. Lamers ◽  
Jonathan P. Tetteh
Keyword(s):  
Seed Germination ◽  
Climate Variability ◽  
Germination Rate ◽  
Dry Weight ◽  
Seed Priming ◽  
Seedling Development ◽  
Hot Water ◽  
Crop Failure ◽  
Simple Method ◽  
Early Season

Agriculture in Mali, a country in Sahelian West Africa, strongly depends on rainfall and concurrently has a low adaptive capacity, making it consequently one of the most vulnerable regions to climate change worldwide. Since early-season drought limits crop germination, and hence growth, ultimately yield during rain-fed depending on production is commonly experienced nowadays in Mali. Germination and establishment of key crops such as the staple sorghum could be improved by seed priming. The effects of hydro-priming with different water sources (e.g., distilled, tap, rain, river, well water) were evaluated respectively for three priming time durations in tepid e.g., at 25 °C (4, 8, and 12 h) and by hot water at 70 °C (in contrast to 10, 20, and 30 min.) in 2014 and 2015. Seed germination and seedling development of nine sorghum genotypes were monitored. Compared to non-primed seed treatments, hydro-priming significantly [p = 0.01] improved final germination percentage, germination rate index, total seedling length, root length, root vigor index, shoot length, and seedling dry weight. The priming with water from wells and rivers resulted in significant higher seed germination (85%) and seedling development, compared to the three other sources of water. Seed germination rate, uniformity, and speed were enhanced by hydro-priming also. It is argued that hydro-priming is a safe and simple method that effectively improve seed germination and seedling development of sorghum. If used in crop fields, the above most promising genotypes may contribute to managing early season drought and avoid failure of seed germination and crop failure in high climate variability contexts.

scholarly journals Muskmelon seed germination and seedling development in response to seed priming

2003 ◽  
Vol 60 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Warley Marcos Nascimento
Keyword(s):  
Seed Germination ◽  
Cucumis Melo ◽  
Germination Rate ◽  
Fungal Growth ◽  
Seed Priming ◽  
Seedling Development ◽  
Factors Affecting ◽  
Total Seed ◽  
Cucumis Melo L ◽  
Osmotic Potentials

Important factors affecting seed priming have not been extensively reported in muskmelon (Cucumis melo L.) studies. The optimization of the seed priming technique becomes very important at the commercial scale. Little information has been reported on seedling development of muskmelon subsequent to seed priming. Seeds of muskmelon were primed in darkness at 25°C in different solutions and three osmotic potentials. Seeds were also primed with and without aeration during different periods. In relation to osmotic solutions, an osmotic potential around -1.30 MPa is most adequate for muskmelon priming. Salt solutions gave better germination rate but were deleterious for seed germination, especially at higher osmotic potentials. Aeration of the soaking salt solution gave faster germination at 17°C, and because of the early germination, these treatments probably presented a better seedling development. Deleterious effect on total seed germination was observed for long soaking periods with aeration. Fungal growth increased on seeds primed in aerated solutions. Seeds from priming treatments had a better germination rate and seedling development under 17 and 25°C.

scholarly journals The efficacy of different seed priming agents for promoting sorghum germination under salt stress

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245505
Author(s):  
Xiaofei Chen ◽  
Ruidong Zhang ◽  
Yifan Xing ◽  
Bing Jiang ◽  
Bang Li ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Germination Rate ◽  
Principal Component ◽  
Dry Weight ◽  
Seed Priming ◽  
Nacl Stress ◽  
Future Research ◽  
Fresh Weight ◽  
Positive Effects

Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.

Alleviation of salt stress using gibberellic acid in Chinese cabbage

2012 ◽  
Vol 60 (4) ◽  
pp. 345-355 ◽  
Author(s):  
M. Jamil ◽  
M. Ashraf ◽  
E. Rha
Keyword(s):  
Salt Stress ◽  
Gibberellic Acid ◽  
Chinese Cabbage ◽  
Germination Rate ◽  
Harmful Effect ◽  
Seed Priming ◽  
Nacl Stress ◽  
Growth And Yield ◽  
Distilled Water ◽  
Membrane Injury

Salinity reduces plant growth and yield by affecting morphological and physiological processes. To alleviate the harmful effects of salt stress various approaches involving plant hormones are used. In this study several parameters involving the measurement of cell membrane injury were used to observe whether stress tolerance could be enhanced in Chinese cabbage (B. oleracea capitata L. Chinensis group) by soaking the seeds for 10 h in distilled water (control), or in 100, 150 or 200 mg l−1 gibberellic acid (GA3). The NaCl concentrations were 0 (control), 50, 100 and 150 mM. Seed treated with GA3 showed increased water uptake and decreased electrolyte leakage as compared to that of distilled water-primed seeds even 24 h after soaking under control conditions. Seed priming with GA3 increased the final germination and the germination rate (1/t50, where t50 is the time to 50% germination) under salt stress conditions. Seed priming also alleviated the harmful effect of salt stress on cabbage in terms of fresh and dry weights. Leaf area was higher in plants raised from seeds primed with the higher GA3 concentrations as compared with those raised from seeds treated with distilled water under control conditions (without NaCl) or at 50 mM NaCl stress. The chlorophyll content increased with the NaCl concentration, especially in plants grown from seeds primed with GA3. Plants grown from GA3-primed seeds also suffered lower cellular injury both under control conditions and under NaCl stress.

scholarly journals The Effect of Hormone Seed Priming Using Gibberellic Acid on Seed Germination Characteristics and Seedling Growth of Coneflower (Echinacea purpurea)

2021 ◽  
Vol 8 (1) ◽  
pp. 173-188
Author(s):  
Farshid Yousefi ◽  
Abdolreza Sihampoosh ◽  
Abdolmahdi Bakhshandeh ◽  
Seyyed Amir Mousavi ◽  
◽  
...  
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Seedling Growth ◽  
Seed Priming ◽  
Echinacea Purpurea ◽  
Germination Characteristics

scholarly journals The Effect of Osmopriming on Seed Germination and Early Seedling Characteristics of Carum carvi L.

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 94
Author(s):  
Iman Mirmazloum ◽  
Attila Kiss ◽  
Éva Erdélyi ◽  
Márta Ladányi ◽  
Éva Zámboriné Németh ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Petri Dish ◽  
Seed Priming ◽  
Germination Percentage ◽  
Positive Effects ◽  
Carum Carvi ◽  
Early Seedling ◽  
Seedling Characteristics

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.

scholarly journals Responses of Seed Germination, Seedling Growth, and Seed Yield Traits to Seed Pretreatment in Maize (Zea maysL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Yu Tian ◽  
Bo Guan ◽  
Daowei Zhou ◽  
Junbao Yu ◽  
Guangdi Li ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Yield ◽  
Seedling Growth ◽  
Germination Rate ◽  
Seed Priming ◽  
Grain Weight ◽  
Shoot Biomass ◽  
Control Group ◽  
Yield Traits ◽  
Seed Pretreatment

A series of seed priming experiments were conducted to test the effects of different pretreatment methods to seed germination, seedling growth, and seed yield traits in maize (Zea maysL.). Results indicated that the seeds primed by gibberellins (GA), NaCl, and polyethylene glycol (PEG) reagents showed a higher imbibitions rate compared to those primed with water. The final germination percentage and germination rate varied with different reagents significantly (P<0.05). The recommended prime reagents were GA at 10 mg/L, NaCl at 50 mM, and PEG at 15% on account of germination experiment. 15% PEG priming reagent increased shoot and root biomass of maize seedling. The shoot biomass of seedlings after presoaking the seeds with NaCl reagent was significantly higher than the seedlings without priming treatment. No significant differences of plant height, leaf number, and hundred-grain weight were observed between control group and priming treatments. Presoaking with water, NaCl (50 mM), or PEG (15%) significantly increased the hundred-grain weight of maize. Therefore, seed pretreatment is proved to be an effective technique to improve the germination performance, seedling growth, and seed yield of maize. However, when compared with the two methods, if immediate sowing is possible, presoaking is recommended to harvest better benefits compared to priming method.

scholarly journals Bioregulators on seed germination and seedling growth of sweet basil

2020 ◽  
Vol 11 ◽  
pp. e3324
Author(s):  
Larissa Santos Castro ◽  
Danúbia Aparecida Costa Nobre ◽  
Daniel Andrés Villegas Hurtado ◽  
Willian Rodrigues Macedo
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Germination Rate ◽  
Ocimum Basilicum ◽  
Shoot Length ◽  
Naphthalene Acetic Acid ◽  
Initial Growth ◽  
Randomized Design ◽  
Root And Shoot Length ◽  
Completely Randomized Design

Basil (Ocimum basilicum L.) plants have multiple uses, ranging from spice to cosmetic purpose, besides being a source of essential oil and aromatic. These plants were commonly propagated by seed, however, few agrotechnologies are used to enhance the seed germination and initial growth in this crop. For this reason, our research aimed to evaluate aspects of seed germination of Ocimum basilicum L. cv. Limoncino, subjected to different bioregulators applied via substrate imbibition. The experiment was carried out in completely randomized design, with four treatments, as follows: T1: control (distilled water), T2: T13 a solution of a product formulated based on indolbutyric acid + naphthalene acetic acid + gibberellic acid (0.2 mL L-1, Liko Química), T3: gibberellic acid (0.5 mM L-1, progibb-400®) and T4: brassinosteroids (0.1 mM L-1). We evaluated: germination rate, germination speed index (GSI), first count, root and shoot length and chlorophyll a, b, total and carotenoids. The bioregulators did not influence germination, first count, GSI and photosynthetic leaf pigment, but application of gibberellic acid and brassinosteroids improve the shoot and root length, respectively. The gibberellic acid and brassinosteroids are promising bioregulators for stimulating initial basil growth.

scholarly journals Sugar apple (Annona squamosa L.) seed germination affected by the application of gibberellins

2016 ◽  
Vol 34 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Fabio Ernesto Martínez M. ◽  
Diego Miranda L. ◽  
Stanislav Magnitskiy
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Positive Response ◽  
Germination Rate ◽  
Germination Percentage ◽  
Annona Squamosa ◽  
Germination Time ◽  
Mean Germination Time ◽  
Germinated Seeds

This research sought to establish the response of the germination percentage (PG), synchrony index (E), mean germination time (MGT) and mean germination rate (MGR) of Annona squamosa L. seeds from Apulo (province of Cundinamarca) and Castilla (province of Tolima), Colombia, to treatments with 0, 50, 100, 200, 400, 600, or 800 mg L-1 of gibberellic acid (GA). All of the treatments with GA increased the PG at each point of time of seed incubation. The 600 mg L-1 GA treatment resulted in higher PGs (92.3% at 16 days for Apulo and 95% at 24 days for Castilla) and lower MGTs (8.75 and 5.38 days for Apulo and Castilla, respectively) than those found with the concentration of 0 mg L-1 GA (17.68 and 10.88 days for Apulo and Castilla, respectively). Also, treating the seeds with 600 mg L-1 GA generated higher MGRs (0.18 and 0.12 germinated seeds/day for Castilla and Apulo, respectively) than those obtained with 0 mg L-1 GA (Castilla = 0.09 and Apulo = 0.06 germinated seeds/ day). Likewise, the germination was synchronized with the application of any concentration of GA. The results evidenced a positive response to the GA application, which provided a tool for the characterization of the phenomenon of dormancy in the A. squamosa seeds.

scholarly journals Seed Priming with Melatonin Improves the Seed Germination of Waxy Maize under Chilling Stress via Promoting the Antioxidant System and Starch Metabolism

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Qingjun Cao ◽  
Gang Li ◽  
Zhengguo Cui ◽  
Fentuan Yang ◽  
Xiaoli Jiang ◽  
...  
Keyword(s):  
Seed Germination ◽  
Antioxidant System ◽  
Germination Rate ◽  
Chilling Stress ◽  
Seed Priming ◽  
Seed Vigor ◽  
Antioxidant Enzyme Activities ◽  
Starch Metabolism ◽  
Waxy Maize ◽  
Significant Difference

Abstract Chilling stress is one of the major abiotic stresses affecting waxy maize plant growth. Melatonin (MT) is able to improve tolerance to abiotic stress in plants. To investigate the effects of seed priming with MT on tolerance to chilling stress in waxy maize, the seed germination characteristics and physiological parameters were tested with varied MT concentrations (0, 50, 100 µM) and treatment times (12, 24 h) at ambient (25 °C) and chilling (13 °C) temperature. MT primed seeds significantly enhanced the germination potential (by 20.29% and 50.71%, respectively), germination rate (by 20.88% and 33.72%), and increased the radicle length (by 90.73% and 217.14%), hypocotyl length (by 60.28% and 136.14%), root length (by 74.59% and 108.70%), and seed vigor index (46.13%, 63.81%), compared with the non-priming seeds under chilling stress. No significant difference was found in priming time between primed and non-primed seeds. In addition, lower H2O2 and malondialdehyde concentrations, increased antioxidant enzyme activities (superoxide dismutase, peroxidase, catalase and ascorbateperoxidase), and promoted starch metabolism were found in primed seeds compared to non-primed ones. It was suggested that seed priming with MT improved waxy maize seed germination under chilling stress through improving antioxidant system and starch metabolism, which protected from oxidative damage.

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