The influence of inhibiting no formation on metabolic recovery of ischemic rat heart by apelin-12

2012 ◽  
Vol 58 (6) ◽  
pp. 702-711 ◽  
Author(s):  
O.I. Pisarenko ◽  
Yu.A. Pelogeykina ◽  
V.S. Shulzhenko ◽  
I.M. Studneva ◽  
Z.D. Bespalova ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Cardiac Function ◽  
Solid Phase ◽  
Adenine Nucleotide ◽  
Energy Charge ◽  
Phase Method ◽  
No Formation ◽  
Rat Hearts ◽  
Solid Phase Method ◽  
Adenine Nucleotide Pool

Apelin 12 (A-12) was synthesized by the automatic solid phase method with use of Fmoc 1H-NMR spectroscopy and mass spectrometry. Effects of apelin-12 (a peptide comprised of 12 aminoacids, A-12) on recovery of energy metabolism and cardiac function were studied in isolated working rat hearts perfused with Krebs buffer (KB) containing 11 mM glucose that were subjected to global ischemia and reperfusion. A short-term infusion of μM 140 A-12 in KB prior to ischemia enhanced myocardial ATP, the total adenine nucleotide pool (ΣAN=ATP+ADP+AMP) and the energy charge of cardiomyocites ((ATP+0.5ADP)/ΣAN) at the end of reperfusion compared with control (KB infusion) and reduced lactate content and lactate/pyruvate ratio in reperfused myocardium to the initial values. This effect was accompanied by improved recovery of coronary flow and cardiac function. Coadministration of 140 μM A-12 and 100 μM L-NAME (the nonspecific NOS inhibitor) profoundly attenuated the peptide influence on metabolic and functional recovery of reperfused hearts. The results indicate involvement of NO, formed under the peptide action, in mechanisms of cardioprotection that are tightly associated with recovery of energy metabolism in postischemic heart.

Hepatic preconditioning preserves energy metabolism during sustained ischemia

2000 ◽  
Vol 279 (1) ◽  
pp. G163-G171 ◽  
Author(s):  
C. Peralta ◽  
R. Bartrons ◽  
L. Riera ◽  
A. Manzano ◽  
C. Xaus ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Adenine Nucleotide ◽  
Adenine Nucleotides ◽  
Energy Charge ◽  
High Energy ◽  
Energetic Cost ◽  
Main Role ◽  
Adenylate Energy Charge ◽  
Glycogen Depletion ◽  
Adenine Nucleotide Pool

We evaluated the possibility that ischemic preconditioning could modify hepatic energy metabolism during ischemia. Accordingly, high-energy nucleotides and their degradation products, glycogen and glycolytic intermediates and regulatory metabolites, were compared between preconditioned and nonpreconditioned livers. Preconditioning preserved to a greater extent ATP, adenine nucleotide pool, and adenylate energy charge; the accumulation of adenine nucleosides and bases was much lower in preconditioned livers, thus reflecting slower adenine nucleotide degradation. These effects were associated with a decrease in glycogen depletion and reduced accumulation of hexose 6-phosphates and lactate. 6-Phosphofructo-2-kinase decreased in both groups, reducing the availability of fructose-2,6-bisphosphate. Preconditioning sustained metabolite concentration at higher levels although this was not correlated with an increased glycolytic rate, suggesting that adenine nucleotides and cAMP may play the main role in the modulation of glycolytic pathway. Preconditioning attenuated the rise in cAMP and limited the accumulation of hexose 6-phosphates and lactate, probably by reducing glycogen depletion. Our results suggest the induction of metabolic arrest and/or associated metabolic downregulation as energetic cost-saving mechanisms that could be induced by preconditioning.

Synthesis and biological activity of new metallocenic angiotensin II analogs

1988 ◽  
Vol 53 (11) ◽  
pp. 2914-2919 ◽  
Author(s):  
Pierrette Maes ◽  
Annie Ricouart ◽  
Emmanuel Escher ◽  
André Tartar ◽  
Christian Sergheraert
Keyword(s):  
Amino Acids ◽  
Biological Activity ◽  
Angiotensin Ii ◽  
Solid Phase ◽  
Rabbit Aorta ◽  
Phase Method ◽  
Solid Phase Method

Analogs of angiotensin II in which phenylalanine in position 8 was replaced with cymantrenylalanine or with its triphenylphosphine photosubstitution product were synthesized by the solid-phase method. On rabbit aorta strips, these peptides were found to be pure antagonists of angiotensin II. Their relative affinities are higher than most other analogs substituted in position 8 with bulky amino-acids.

Synthesis of four new V1 antagonists of arginine-vasopressin (AVP) containing new thioacids at position 1 and their vasoconstrictor activity towards isolated mesenteric arterial vessels of rats

1991 ◽  
Vol 56 (2) ◽  
pp. 491-498 ◽  
Author(s):  
Bernard Lammek ◽  
Izabela Derdowska ◽  
Tomasz M. Wierzba ◽  
Witold Juzwa
Keyword(s):  
Peptide Synthesis ◽  
Arginine Vasopressin ◽  
Solid Phase ◽  
Structural Features ◽  
Phase Method ◽  
Competitive Antagonist ◽  
Vasoconstrictor Effect ◽  
Solid Phase Method

In an attempt to determine some of the structural features in position 1 that account for V1 antagonism, four new analogues of arginine-vasopressin were synthesized and the effect of the modifications on the vasoconstrictor activity was checked using isolated mesenteric arterial vessels of rats. The protected precursors required for these analogues were synthesized by a solid phase method of peptide synthesis. One of the reported analogues, namely [1-(4-mercapto-4-tetrahydrothiopyraneacetic acid)., 2-O-methyltyrosine, 8-arginine]vasopressin appears to be a potent competitive antagonist of the vasoconstrictor effect by AVP.

Synthesis of a 21-Residue Fragment of Human Proinsulin by the Polyamide Solid Phase Method

1981 ◽  
Vol 362 (2) ◽  
pp. 833-840 ◽  
Author(s):  
Eric ATHERTON ◽  
Willy HÜBSCHER ◽  
Robert C. SHEPPARD ◽  
Vivienne WOOLLEY
Keyword(s):  
Solid Phase ◽  
Phase Method ◽  
Human Proinsulin ◽  
Solid Phase Method

scholarly journals Syntheses of two deacetyl-thymosin β4analogs and their anti-inflammatory effect on carrageenin-induced edema in the mouse paw

2001 ◽  
Vol 10 (2) ◽  
pp. 89-92 ◽  
Author(s):  
T. Abiko ◽  
R. Ogawa
Keyword(s):  
Marked Reduction ◽  
Solid Phase ◽  
The Other ◽  
Para Position ◽  
Phase Method ◽  
Antiinflammatory Effect ◽  
Solid Phase Method ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Two {Met(0)6}deacetyl-thymosin β4analogs containing Phe(4F) or Tyr(Me) at position 12 were synthesized by the manual solid-phase method, and their anti-inflammatory effect on carrageenin-induced edema in the mouse paw was studied. Fluorination of the para-position of Phe12resulted in a marked antiinflammatory effect on carrageenin-induced edema in the mouse paw compared with that of our synthetic {Met(0)6}deacetyl-thymosin β4, but the other analog, {Met(0)6, Tyr(Me)12}deacetyl-thymosin β4, showed a marked reduction of the anti-inflammatory effect.

The Gas-Solid-Phase 2,5-Dioxopiperazine Synthesis. Cyclization of Vaporous Dipeptides on Silica Surface

1994 ◽  
Vol 59 (2) ◽  
pp. 461-466 ◽  
Author(s):  
Vladimir A. Basiuk ◽  
Taras Yu. Gromovoy
Keyword(s):  
Silica Surface ◽  
Solid Phase ◽  
Phase Method ◽  
Solid Phase Method

The "gas solid-phase" method is used for the preparation of both symmetric and asymmetric 2,5-dioxopiperazines via cyclization of vaporous linear dipeptides in the presence of silica.

Combustion Synthesis of La0.65Sr0.35MnO3 and its Sensing Properties for NO2

2016 ◽  
Vol 680 ◽  
pp. 208-211
Author(s):  
Lian Lian Wu ◽  
Qiang Li ◽  
Dan Yu Jiang ◽  
Jin Feng Xia
Keyword(s):  
Solid Phase ◽  
High Sensitivity ◽  
Combustion Method ◽  
Fast Response ◽  
Size Analysis ◽  
Phase Method ◽  
X Ray Diffraction ◽  
Ultrafine Structure ◽  
Nox Sensor ◽  
Solid Phase Method

In this paper, La0.65Sr0.35MnO3 (LSM) oxide powder with ultrafine structure has been synthesized by self-propagating combustion method. The powders were characterized by X-ray diffraction, scanning electron microscopy and laser size analysis. Compared to the powders prepared by traditional solid-phase method, the grain size of powders prepared by self-propagating combustion method is relatively small and uniform. Starting from ultrafine LSM powders, sensing electrode (SE) for NO2 mixed-potential sensors based on yttria-stablized zirconia (YSZ) was fabricated. As-obtained NO2 sensor displays fast response and high sensitivity (25.4mV/decade). The response values of the sensor have good linear relationship with the logarithm of NO2 concentration varying from 30ppm to 500ppm.Keywords:Self-propagating combustion method; La0.65Sr0.35MnO3; NOx sensor; YSZ

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