Clinical and immunological characterization of perilla seed allergy in children

Background: Perilla seeds are known to cause immediate allergic reactions. However, reports on perilla seed allergies are limited to a few case reports worldwide, and there is currently no diagnostic test for such allergies. Objective: Our objective was to analyze the clinical and immunological characteristics of perilla seed allergy and to identify allergens for the development of diagnostic methods. Methods: Twenty-one children with clinical perilla seed allergy were enrolled from two tertiary hospitals between September 2016 and June 2019. Using perilla seed extract, we developed a skin prick test (SPT) reagent and an IgE enzyme-linked immunosorbent assay (ELISA) for perilla seed allergy diagnosis. IgE immunoblotting was performed for identifying putative allergenic components, and amino acid composition analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results: The median age of children with perilla seed allergy was 3 years; the proportion of children with anaphylaxis was 28.6%. Perilla seed SPT was performed in 15 of 21 children, all of whom tested positive. On ELISA, 85.7% of children tested positive for perilla seed-specific IgE. Proteins with molecular weights of 50, 31–35, and 14–16 kDa showed binding with the sera of >50% of children with perilla seed allergy. LC-MS/MS analysis of these three protein fractions indicated 8 putative proteins, including perilla oleosin (Accession No. 9963891), to be allergens. Conclusion: This study documented the clinical characteristics and immunological profiles of 21 children with perilla seed allergy. Our results suggest that oleosin is one of the major allergens in perilla seeds.

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Overcoming Shellfish Allergy: How Far Have We Come?

International Journal of Molecular Sciences ◽

10.3390/ijms21062234 ◽

2020 ◽

Vol 21 (6) ◽

pp. 2234 ◽

Cited By ~ 1

Author(s):

Christine Y.Y. Wai ◽

Nicki Y.H. Leung ◽

Ka Hou Chu ◽

Patrick S.C. Leung ◽

Agnes S.Y. Leung ◽

...

Keyword(s):

Treatment Options ◽

Standard Of Care ◽

Specific Ige ◽

Diagnostic Methods ◽

Asia Pacific ◽

Prick Test ◽

Immunological Responses ◽

The Past ◽

Molecular Features ◽

Shellfish Allergy

Shellfish allergy caused by undesirable immunological responses upon ingestion of crustaceans and mollusks is a common cause of food allergy, especially in the Asia-Pacific region. While the prevalence of shellfish allergy is increasing, the mainstay of clinical diagnosis for these patients includes extract-based skin prick test and specific IgE measurement while clinical management consists of food avoidance and as-needed use of adrenaline autoinjector should they develop severe allergic reactions. Such a standard of care is unsatisfactory to both patients and healthcare practitioners. There is a pressing need to introduce more specific diagnostic methods, as well as effective and safe therapies for patients with shellfish allergy. Knowledge gained on the identifications and defining the immuno-molecular features of different shellfish allergens over the past two decades have gradually translated into the design of new diagnostic and treatment options for shellfish allergy. In this review, we will discuss the epidemiology, the molecular identification of shellfish allergens, recent progress in various diagnostic methods, as well as current development in immunotherapeutic approaches including the use of unmodified allergens, hypoallergens, immunoregulatory peptides and DNA vaccines for the prevention and treatment of shellfish allergy. The prospect of a “cure “for shellfish allergy is within reach.

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IgE reactivity to fish allergens from Pacific cod (Gadus macrocephalus) in atopic dogs

10.21203/rs.2.19058/v4 ◽

2020 ◽

Author(s):

Ichiro Imanishi ◽

Jumpei Uchiyama ◽

Keijiro Mizukami ◽

Junichi Kamiie ◽

Keigo Kurata ◽

...

Keyword(s):

Specific Ige ◽

Enzyme Linked Immunosorbent Assay ◽

Pacific Cod ◽

Fish Allergy ◽

Ige Reactivity ◽

Pacific Cod Gadus Macrocephalus ◽

Gadus Macrocephalus ◽

The Pacific ◽

Allergenic Proteins ◽

Liquid Chromatography Tandem Mass

Abstract Background: IgE reactivity to fish allergens in atopic dogs, which are used as models for food allergy, has not been elucidated to date. We investigated IgE reactivity to crude extracts and purified allergens derived from the Pacific cod (Gadus macrocephalus) in atopic dogs to identify the allergenic proteins of cod.Results: The levels of specific IgE to crude cod extracts were measured in the sera of 179 atopic dogs, including 27 dogs with cod allergy, using enzyme-linked immunosorbent assay (ELISA). Specific IgE to crude cod extracts were present in 36 (20%) of the 179 atopic dogs and in 12 (44%) of the 27 dogs with cod allergy. The allergens in crude cod extracts were analyzed by ELISA, immunoblotting, and liquid chromatography-tandem mass spectrometry. In allergen component analysis, IgE reactivity to tropomyosin and enolase was observed in the sera of dogs with cod allergy. IgE reactivity to parvalbumin, collagen, and tropomyosin was evaluated using the sera of atopic dogs that tested positive for specific IgE to crude cod extracts. Among the 36 dogs with IgE reactivity to crude cod extracts, 9 (25%), 14 (39%), and 18 (50%) dogs tested positive for specific IgE to parvalbumin, collagen, and tropomyosin, respectively.Conclusions: The IgE reactivity to cod allergens observed in dogs was similar to that in humans, and this finding further supports the use of atopic dogs with fish allergy as a model for fish allergy in humans.

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IgE reactivity to fish allergens from Pacific cod (Gadus macrocephalus) in atopic dogs

BMC Veterinary Research ◽

10.1186/s12917-020-02559-1 ◽

2020 ◽

Vol 16 (1) ◽

Author(s):

Ichiro Imanishi ◽

Jumpei Uchiyama ◽

Keijiro Mizukami ◽

Junichi Kamiie ◽

Keigo Kurata ◽

...

Keyword(s):

Specific Ige ◽

Enzyme Linked Immunosorbent Assay ◽

Pacific Cod ◽

Fish Allergy ◽

Ige Reactivity ◽

Pacific Cod Gadus Macrocephalus ◽

Gadus Macrocephalus ◽

The Pacific ◽

Allergenic Proteins ◽

Liquid Chromatography Tandem Mass

Abstract Background IgE reactivity to fish allergens in atopic dogs, which are used as models for food allergy, has not been elucidated to date. We investigated IgE reactivity to crude extracts and purified allergens derived from the Pacific cod (Gadus macrocephalus) in atopic dogs to identify the allergenic proteins of cod. Results The levels of specific IgE to crude cod extracts were measured in the sera of 179 atopic dogs, including 27 dogs with cod allergy, using enzyme-linked immunosorbent assay (ELISA). Specific IgE to crude cod extracts were present in 36 (20%) of the 179 atopic dogs and in 12 (44%) of the 27 dogs with cod allergy. The allergens in crude cod extracts were analyzed by ELISA, immunoblotting, and liquid chromatography-tandem mass spectrometry. In allergen component analysis, IgE reactivity to tropomyosin and enolase was observed in the sera of dogs with cod allergy. IgE reactivity to parvalbumin, collagen, and tropomyosin was evaluated using the sera of atopic dogs that tested positive for specific IgE to crude cod extracts. Among the 36 dogs with IgE reactivity to crude cod extracts, 9 (25%), 14 (39%), and 18 (50%) dogs tested positive for specific IgE to parvalbumin, collagen, and tropomyosin, respectively. Conclusions The IgE reactivity to cod allergens observed in dogs was similar to that in humans, and this finding further supports the use of atopic dogs with fish allergy as a model for fish allergy in humans.

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IgE reactivity to fish allergens in Pacific cod (Gadus macrocephalus) in atopic dogs

10.21203/rs.2.19058/v2 ◽

2020 ◽

Author(s):

Ichiro Imanishi ◽

Jumpei Uchiyama ◽

Keijiro Mizukami ◽

Junichi Kamiie ◽

Keigo Kurata ◽

...

Keyword(s):

Specific Ige ◽

Enzyme Linked Immunosorbent Assay ◽

Pacific Cod ◽

Fish Allergy ◽

Ige Reactivity ◽

Pacific Cod Gadus Macrocephalus ◽

Chromatography Tandem Mass Spectrometry ◽

Gadus Macrocephalus ◽

Allergenic Proteins ◽

Liquid Chromatography Tandem Mass

Abstract Background: IgE reactivity to fish allergens in atopic dogs, which are used as models for food allergy, has not been elucidated to date. We investigated IgE reactivity to crude extracts and purified Pacific cod (Gadus macrocephalus) allergens in atopic dogs to identify the allergenic proteins of cod.Methods: Specific IgE to crude cod extracts in the sera of 179 atopic dogs, including 27 dogs with cod allergy, were measured using enzyme-linked immunosorbent assay (ELISA). The allergens of crude cod extracts were analyzed by ELISA, immunoblotting, and liquid chromatography-tandem mass spectrometry (LC-MS/MS). IgE reactivity to parvalbumin, collagen, and tropomyosin was evaluated using the sera of atopic dogs that were positive for specific IgE to crude cod extracts.Results: Specific IgE to crude cod extracts were present in 36 (20%) of the 179 atopic dogs and 12 (44%) of the 27 dogs with cod allergy. In allergen component analysis, IgE reactivity to tropomyosin and enolase was observed in the sera of dogs with cod allergy. Among the 36 dogs with IgE reactivity to crude cod extracts, 9 (25%), 14 (39%), and 18 (50%) dogs had specific IgE to parvalbumin, collagen, and tropomyosin, respectively.Conclusions: The dogs exhibited IgE reactivity to the cod allergens which observed in humans, providing support for the use of atopic dogs with fish allergy as a model for fish allergy in humans.

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IgE reactivity to fish allergens from Pacific cod (Gadus macrocephalus) in atopic dogs

10.21203/rs.2.19058/v3 ◽

2020 ◽

Author(s):

Ichiro Imanishi ◽

Jumpei Uchiyama ◽

Keijiro Mizukami ◽

Junichi Kamiie ◽

Keigo Kurata ◽

...

Keyword(s):

Specific Ige ◽

Enzyme Linked Immunosorbent Assay ◽

Pacific Cod ◽

Fish Allergy ◽

Ige Reactivity ◽

Pacific Cod Gadus Macrocephalus ◽

Gadus Macrocephalus ◽

The Pacific ◽

Allergenic Proteins ◽

Liquid Chromatography Tandem Mass

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Positive associations between infections ofToxoplasma gondiiand seropositivity withAnisakis simplexin human patients suffering from chronic urticaria

Journal of Helminthology ◽

10.1017/s0022149x14000480 ◽

2014 ◽

Vol 89 (6) ◽

pp. 707-713 ◽

Cited By ~ 1

Author(s):

V. Fernández-Fígares ◽

M. Rodero ◽

A. Valls ◽

C. De Frutos ◽

A. Daschner ◽

...

Keyword(s):

Chronic Urticaria ◽

Odds Ratio ◽

Specific Ige ◽

Anisakis Simplex ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Atopic Sensitization ◽

Prick Test ◽

Food Borne ◽

The Relationship

AbstractToxoplasma gondiiis a food-borne and orofecal microorganism which produces chronic infection, and attempts have been made to prove its negative association with atopy in the context of the hygiene hypothesis.Anisakis simplexis a fish parasite associated with chronic urticaria (CU) in endemic regions. We analysed the relationship between both infectious agents in CU. We included 42 patients with chronic urticaria (18 patients with CU associated withA. simplexsensitization and 24 not sensitized CU patients). Patients were assessed for atopy by a skin prick test (SPT) against common aeroallergens and for respiratory symptoms.Anisakis simplexsensitization was assessed by SPT and specific IgE by CAP fluoro-enzyme immunoassay (CAP-FEIA). Anti-T. gondiiIgG levels were measured by enzyme-linked immunosorbent assay (ELISA). CU patients were analysed with respect toT. gondiiseropositivity,A. simplexsensitization, atopy and immigrant status. The seroprevalence ofT. gondiiwas 40.5% in CU patients and 42.1% in the control group. Immigrants were more frequently infected byT. gondii(41.2% versus 12%;P =0.036). Anti-T. gondiiIgG antibodies were associated with pastA. simplexparasitism (odds ratio 6.73;P =0.03) and independently with atopic sensitization (odds ratio 5.85;P =0.04). In CU patients,T. gondiihas no protective effect on atopic sensitization orA. simplexsensitization.

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IgE reactivity to components of fish allergens in Pacific cod (Gadus macrocephalus) in atopic dogs

10.21203/rs.2.19058/v1 ◽

2019 ◽

Author(s):

Ichiro Imanishi ◽

Jumpei Uchiyama ◽

Keijiro Mizukami ◽

Junichi Kamiie ◽

Keigo Kurata ◽

...

Keyword(s):

Specific Ige ◽

Enzyme Linked Immunosorbent Assay ◽

Pacific Cod ◽

Fish Allergy ◽

Ige Reactivity ◽

Allergen Components ◽

Pacific Cod Gadus Macrocephalus ◽

Chromatography Tandem Mass Spectrometry ◽

Gadus Macrocephalus ◽

Liquid Chromatography Tandem Mass

Abstract Background IgE reactivity to fish allergens in atopic dogs, which are used as models for food allergy, has not been elucidated to date. We investigated IgE reactivity to crude and purified Pacific cod (Gadus macrocephalus) allergens in atopic dogs to identify the allergenic components of cod. Methods Specific IgE to crude cod allergens in the sera of 179 atopic dogs, including 27 dogs with cod allergy, were measured using enzyme-linked immunosorbent assay (ELISA). The allergenic components of crude cod antigen were analyzed by ELISA, immunoblotting, and liquid chromatography-tandem mass spectrometry (LC-MS/MS). IgE reactivity to parvalbumin, collagen, and tropomyosin was evaluated using the sera of atopic dogs that were positive for specific IgE to crude cod allergens. Results Specific IgE to crude cod allergens were present in 36 (20%) of the 179 atopic dogs and 12 (44%) of the 27 dogs with cod allergy. In allergen component analysis, IgE reactivity to tropomyosin and enolase was observed in the sera of dogs with cod allergy. Among the 36 dogs with IgE reactivity to crude cod extracts, 9 (25%), 14 (39%), and 18 (50%) dogs had specific IgE to parvalbumin, collagen, and tropomyosin, respectively. Conclusions The dogs exhibited IgE reactivity to the cod allergen components similar to that observed in humans, providing support for the use of atopic dogs with fish allergy as a model for fish allergy in humans.

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ORAL ALLERGY SYNDROME (OAS) AKIBAT REAKSI ALERGI MAKANAN

B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah ◽

10.33854/jbdjbd.20 ◽

2018 ◽

Vol 2 (2) ◽

pp. 125-132

Author(s):

Nanan Nur'aeny

Keyword(s):

Fruits And Vegetables ◽

Immunoglobulin E ◽

Signs And Symptoms ◽

Basophil Activation Test ◽

Specific Ige ◽

Oral Allergy Syndrome ◽

Enzyme Linked Immunosorbent Assay ◽

Type I ◽

Prick Test ◽

Bet V 1

Allergic condition such as itching of the skin, or runny nose and sneezing are widely known, but different case of allergic reaction in the mouth. Are still unknown, one of them is Oral Allergy Syndrome (OAS). Almost everyone knows food allergens such as shrimp, or marine fish, but actually fresh fruits and vegetables also can trigger allergic reactions like OAS in the form of itching or swelling of the lips, tongue, palate, and pharynx. Author intends to provide further information about the OAS in this paper. The mechanism of OAS is a type I, immunoglobulin E-mediated hypersensitivity reaction, common in atopic people with history of atopic rhinitis, bronchial asthma, or urticaria. Food allergen that causes OAS have a cross reaction/ homolog with pollen from protein pathogen respons / PR-10 family, such as Bet v 1 (Birch) and Bet v 2 (Birch). Some OAS allergens from vegetables that homolog with Bet v 1 includes celery (Api g 1) and soybean (Gly m 4), and other allergens from fruits homolog with Bet v 2, such as Ana c1 alergen pineapple. The diagnosis of OAS based on medical history, clinical examination and diagnostic tests includes skin prick test, specific IgE serum, or basophil activation test, and cellular antigen stimulation test with an enzyme-linked immunosorbent assay. Management of OAS consists of non-pharmacological includes information and education to avoid the causes, and give suggestion to eat cooked food. Pharmacologically are prescription of antihistamines or epinephrine injection in case of emergencies. Signs and symptoms of OAS in fact have long been found, but its diagnosis and management are still not widely known. Dentist should know about signs and symptoms of food allergy in the oral mucosa include OAS which caused by fruits and vegetables such as celery, soybean, carrot, apple, pineapple, and strawberry, so finally dentist will provide good management for the patient..

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Oral allergy syndrome by fruit and vegetable PR-10 allergy: Accuracy of in vivo diagnosis

Allergologia et Immunopathologia ◽

10.15586/aei.v49i2.28 ◽

2021 ◽

Vol 49 (2) ◽

pp. 129-132

Author(s):

Cristina De Rose ◽

Maria Letitzia Patti ◽

Alessadro Gambacorta ◽

Federica Brancato ◽

Stefano Miceli Sopo

Keyword(s):

Specific Ige ◽

Fruit And Vegetables ◽

Diagnostic Methods ◽

Oral Allergy Syndrome ◽

In Vitro Tests ◽

Prick Test ◽

Fresh Food ◽

In Vivo Diagnosis

Routine diagnostic methods for allergies to plant-derived foods are based on skin prick test (SPT) with commercial extracts, prick-by-prick (PbP) with fresh food, serum-specific IgE measurement, and oral food challenge.We discuss the possibility and the advantages of performing, in patients with oral allergy syn-drome (OAS) by fruit and vegetables (excluding nuts) PR-10 allergy, component-resolved diag-nosis (CRD) by SPT and PbP with raw and cooked vegetables, rather than performing a CRD with in vitro tests by drawing blood.Based on our clinical experience and the studies published in the literature, we believe that, at least for the OAS by fruit and vegetables (excluding nuts) PR-10 allergy, the search for sensitizing allergens and related cross-reactive allergens with SPT and PbP can be performed routinely in clinical practice, even at the primary-care level.

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Real-World Data Evaluation Of Total IgE, Specific IgE And Omalizumab Therapy In A Cohort Of Allergic Asthma Patients Treated In A Community-Based Practice

Journal of Immunology and Allergy ◽

10.37191/mapsci-2582-6549-1(3)-016 ◽

2020 ◽

Author(s):

Fortune O Alabi

Keyword(s):

Allergic Asthma ◽

Specific Ige ◽

Forced Expiratory Volume ◽

Act Scores ◽

Asthma Control Test ◽

Real World Data ◽

Total Ige ◽

Prick Test ◽

Negative Results ◽

Asthma Patients

Objective: In this study, we: (1) evaluated the correlation between total IgE and the presence of specific IgE; (2) compared the characteristics of patients with positive specific IgE to those with negative specific IgE; and, (3) analyzed the allergic testing results of patients on omalizumab and reported the effect of omalizumab on forced expiratory volume (FEV1) and asthma control test (ACT) results. Methods: Data from patients diagnosed with allergic asthma and seen at Florida Lung, Asthma & Sleep Specialists (FLASS) between January 2016 and June 2019 were analyzed. Parameters evaluated were total IgE, and levels of specific IgE to antigens in the ImmunoCAP test and skin prick test (SPT). Additional parameters for patients on omalizumab therapy for at least 6 months were FEV1, % predicted FEV1 and ACT results. Results: A total of 475 patients (114 males, 361 females) met the inclusion criteria. The mean age was 53 years (range: 17 to 89 years). Of these, 36 patients were not included in the analysis due to incomplete data. Mean total IgE was higher in patients with positive ImmunoCAP results compared to those with negative results (396 KU/L vs. 81.3 KU/L). There was a significant positive correlation between total IgE and levels of positive specific IgE in the ImmunoCAP test (p<0.0001, r=0.36, n=213 patients). The correlation between total IgE and levels of positive allergens in SPT was not significant (p=0.15, n=44 patients) Two positive reactions to allergens were seen in 22% of ImmunoCAP tests and 13% of SPT tests. There was no statistically significant improvement in FEV1 (p=0.097, CI -0.17 to 0.02) and % predicted FEV1 (p=0.109, CI -6.63 to 0.70) in patients who used omalizumab for at least 6 months. There was a statistically significant improvement in ACT scores (p=0.031, CI -4.21 to -0.21) in patients who used omalizumab for at least 6 months. Conclusion: Allergic asthma could be seen in patients who had an absence of specific IgE in ImmunoCAP and a negative reaction to SPT. The benefit of omalizumab therapy is not limited to allergic asthma patients with positive specific IgE.

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