scholarly journals Effect of autologous platelet rich plasma on anti-mullerian hormone and antral follicle count in sub fertile women with poor ovarian reserve

2022 ◽  
Author(s):  
Muhammad J. Uddin ◽  
Jesmine Banu ◽  
Shakeela Ishrat ◽  
Sabiha Sultana ◽  
Serajoom Munira ◽  
...  
Keyword(s):  
Ovarian Reserve ◽  
Cellular Proliferation ◽  
Platelet Rich Plasma ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Base Line ◽  
Safe Procedure ◽  
Ovarian Reserve Markers ◽  
Poor Ovarian Reserve ◽  
Fertile Women

Background: Ovarian aging may be reversible. Platelet rich plasma (PRP) has growth factors that promote cellular proliferation and folliculogenesis. Recently published studies and case reports suggest that ovarian rejuvenation can be done by PRP treatment. The objective of the study was to evaluate the effect of platelet rich plasma on ovarian reserve markers such as anti mullerian hormone (AMH) and antral follicle count (AFC) in sub fertile women with poor ovarian reserve (POR).Methods: The self-controlled quasi experimental study was carried out on 29 sub fertile women with poor ovarian reserve. They were selected for laparoscopic tubo-peritoneal evaluation as they could not afford in vitro fertilization. During laparoscopy, 5 ml of pre prepared autologous PRP was injected into each ovary. Post-PRP AMH and AFC were measured at every cycle for a period of at least three (3) months and compared with base line values.Results: Mean age of participants was 35.9±3.2 years. Baseline AMH was 0.31±0.17 ng/ml and baseline AFC was 3.41±0.73. AMH was raised on first, second and third cycle from base line values in 58.62%, 86.21% and 91.30% of the study population respectively. AMH changes in all three cycle were statistically significant. Pregnancy occurred in three (10.34%) women during the study period.Conclusions: The injection of autologous PRP into human ovaries is a safe procedure to improve ovarian reserve markers (AMH and AFC) in women with POR.

Role of ovarian reserve markers, antimüllerian hormone and antral follicle count, as aneuploidy markers in ongoing pregnancies and miscarriages

2015 ◽  
Vol 103 (5) ◽  
pp. 1221-1227.e2 ◽  
Author(s):  
Maribel Grande ◽  
Virginia Borobio ◽  
Mar Bennasar ◽  
Iosifina Stergiotou ◽  
Immaculada Mercadé ◽  
...  
Keyword(s):  
Ovarian Reserve ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Antimullerian Hormone ◽  
Antimüllerian Hormone ◽  
Ovarian Reserve Markers

Female adolescents and young women previously treated for pediatric malignancies: assessment of ovarian reserve and gonadotoxicity risk stratification for early identification of patients at increased infertility risk

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Francesca Parissone ◽  
Rossana Di Paola ◽  
Rita Balter ◽  
Simone Garzon ◽  
Stefano Zaffagnini ◽  
...  
Keyword(s):  
Ovarian Reserve ◽  
Early Identification ◽  
Young Patients ◽  
Risk Groups ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Ovarian Insufficiency ◽  
Adolescent Cancer ◽  
Previously Treated ◽  
Ovarian Reserve Markers

AbstractBackgroundChildhood and adolescent cancer survivors (CACSs) are at risk of adverse reproductive outcomes. Assessment of follicle-stimulating hormone (FSH) levels is the most common test used to diagnose premature ovarian insufficiency (POI) whereas anti-m|llerian hormone (AMH) and antral follicle count (AFC) have been proposed as ovarian reserve markers. We assessed the correlation between these markers and treatment gonadotoxicity risk (GR) in CACSs.Subject and methodsA total of 55 female CACSs were enrolled. GR was graded as low, medium, or high according to classifications based on disease and treatments and on cyclophosphamide equivalent dose score. FSH, AMH, and AFC were determined. POI was defined by amenorrhea and FSH>30 IU/L. For remaining patients, diminished ovarian reserve (DOR) was defined by AMH<5th centile. FSH and AFC cut-offs proposed in the literature as DOR markers were also considered (FSH>10 IU/L or >95th centile; AFC<8 or <5th centile).ResultsOvarian reserve results to be compromised in 23 (41.8%) patients: 14 with DOR and 9 with an established POI. Results showed GR classifications to be a good predictor of ovarian reserve, with significantly lower AMH and AFC in the high-risk groups. AFC resulted to be directly correlated with AMH (r=0.75, p<0.001).ConclusionsIn CACSs, GR classifications correlate with post-treatment AMH and AFC. These are useful tools in the early identification of young patients with DOR, who may benefit from reproductive and fertility preservation counseling. Further studies are needed to determine the rate and time of progression from DOR to POI in this population.

Pituitary-ovary axis and ovarian reserve in fertile women with multiple sclerosis: A pilot study

2015 ◽  
Vol 22 (4) ◽  
pp. 564-568 ◽  
Author(s):  
Maria Sepúlveda ◽  
Cristina Ros ◽  
Elena H Martínez-Lapiscina ◽  
Nuria Solà-Valls ◽  
Mariona Hervàs ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Disease Activity ◽  
Ovarian Reserve ◽  
Reproductive Potential ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Gonadal Hormones ◽  
Relapsing Remitting ◽  
Ovary Function ◽  
Ovarian Reserve Markers

Since a decline in the ovary function might impact the reproductive potential in women with multiple sclerosis (MS), we investigated the pituitary-ovary axis and ovarian reserve, including anti-Müllerian hormone (AMH) levels and ultrasound imaging of the ovaries, of 25 relapsing–remitting MS patients and 25 age-matched healthy controls. Mean levels of pituitary-gonadal hormones and age-adjusted parameters of ovarian reserve markers were not significantly different between both groups. Patients with higher disease activity (annualized relapse rate >0.5; n=9) had significantly lower AMH levels, total antral follicle count and ovarian volume, than those with lower disease activity. The finding of poorer ovarian reserve associated with higher disease activity should be taken into consideration since it may negatively impact the reproductive prognosis.

Reduced Ovarian Reserve in Patients with Takayasu Arteritis

2014 ◽  
Vol 41 (10) ◽  
pp. 2055-2059 ◽  
Author(s):  
Andrea Rocha S. Mont’Alverne ◽  
Rosa Maria R. Pereira ◽  
Lucas Yugo S. Yamakami ◽  
Vilma Santos T. Viana ◽  
Edmund Chada Baracat ◽  
...  
Keyword(s):  
Cumulative Dose ◽  
Takayasu Arteritis ◽  
Ovarian Reserve ◽  
Statistical Significance ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Healthy Controls ◽  
Reactive Protein ◽  
Demographical Data ◽  
Ovarian Reserve Markers

Objective.To assess ovarian reserve markers in patients with Takayasu arteritis (TA).Methods.Twenty patients with TA and 24 healthy controls were evaluated for ovarian reserve by follicle-stimulating hormone, luteinizing hormone, and estradiol, and antral follicle count (AFC). Anti-Müllerian hormone (AMH) was measured by ELISA using 2 different kits. Demographical data, menstrual abnormalities, disease variables, and treatment were also analyzed.Results.The median current age was similar in patients with TA and controls (31.2 ± 6.1 vs 30.4 ± 6.9 yrs, p = 0.69). The frequencies of decreased levels of AMH in patients with TA were identical using both kits and higher when compared to controls (50% vs 17%, p = 0.02; 50% vs 19%, p = 0.048). A positive correlation was observed between the 2 kits in patients with TA (r = +0.93, p < 0.0001) and in healthy controls (r = +0.93, p < 0.0001). The apparent lower AFC (11 vs 16, p = 0.13) and the higher frequency of low AFC (41% vs 22%, p = 0.29) in TA compared to controls did not reach statistical significance. Other hormones were similar in both groups (p > 0.05). Further evaluation of patients with TA with low AMH levels (< 1.0 ng/ml) versus normal AMH levels (> 1.0 ng/ml) revealed that the frequency of current disease activity (p = 1.0) and the median of erythrocyte sedimentation rate (p = 0.6), C-reactive protein (p = 0.4), prednisone cumulative dose (p = 0.8), and methotrexate cumulative dose (p = 0.8) were comparable in both groups. Cyclophosphamide use was reported in only 1 patient with reduced ovarian reserve, whereas none of the remaining patients received gonadotoxic drugs.Conclusion.To the best of our knowledge, our present study was the first to suggest that patients with TA may have diminished ovarian reserve.

scholarly journals Effect of Ayurvedic Treatment Protocol on Diminishing Ovarian Reserve

2020 ◽  
Vol 11 (2) ◽  
pp. 228-234
Author(s):  
Divya U ◽  
Vijayakumar N
Keyword(s):  
Ovarian Reserve ◽  
Reproductive Potential ◽  
Treatment Protocol ◽  
Antral Follicle ◽  
Poor Responder ◽  
Antral Follicle Count ◽  
In Vitro Fertilisation ◽  
Rank Test ◽  
Ayurvedic Treatment

Diminishing ovarian reserve (DOR) is a condition in which the ovary loses reproductive potential, compromising fertility. Nowadays 10-30% of female infertility is due to DOR and considered as “expected poor responder” for In vitro fertilisation (IVF).   Correlation of DOR can be done with Dathukshaya vandya (depletion or inadequate formation of dhatus) explained in Harithasamhita. The objective of the study was to evaluate the effect of Ayurvedic treatment protocol on Diminishing ovarian reserve. The study protocol includes- ashtachurna for deepana (appetiser), pachana (digestives) and kolakulathadi churna for udwarthana (powder massage). Sukumaragrutha used for snehapana (oral administration of medicated ghee), utharabasthi (intrauterine administration) and rasayana (rejuvenation therapy ). Danwantarathaila abhyanga (oleation) and ooshmasweda (sudation) done for 3 days. Sukumaraeranda was used for virechana (therapeutic purgation) and also Yogabasthi (medicated enema). The study design was pre and post interventional study with a sample size of 15 selected as per inclusion and exclusion criteria, conducted at hospital for women and children, Government Ayurveda College, Thiruvananthapuram. Assessment was based on Bologna criteria for DOR. The statistical techniques employed are Wilcoxon’s signed rank test and Paired t test. Results showed statistically significant effect on improving Antral follicle count (AFC) (p- 0.01), Estradiol (p- 0.005), conception (p- 0.014), on regulating amount of bleeding (p- 0.003), menstrual interval correction (p-0.001) and dyspareunia (p-0.005). But insignificant effect on improving Anti Mullerian Hormone (AMH) (p- 0.469) and regularising LH/FSH ratio (p-0.104) was found.

Value of serum and follicular fluid Sirt1,Sirt2 protein levels in predicting the outcome of assisted reproduction

2020 ◽  
Author(s):  
Lingnv Yao ◽  
Wenqin Lin ◽  
Nan Jiang ◽  
Chuyan Li ◽  
Haifeng Cao ◽  
...  
Keyword(s):  
Assisted Reproduction ◽  
Follicular Fluid ◽  
Ovarian Reserve ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Advanced Age ◽  
Control Group ◽  
Age Group ◽  
Protein Levels ◽  
Study Population

Abstract Background: To explore whether serum and follicular fluid Sirt1, Sirt2 can reflect ovarian reserve and predict the outcome of assisted reproduction. Methods: The study population comprised 125 patients , 39 in OPOI（occult form of premature ovarian insufficiency）group, 49 in advanced age group , and 37 in control group. The levels of serum Sirt1, Sirt2 were measured on the 2nd to 5th day of menstruation (bSirt1,bSirt2) and HCG day. Follicular fluid Sirt1 (FFSirt1) and Sirt2 (FFSirt2), were determined on OPU (oocyte pick up) day. Results: The level of FFSirt2 in the advanced age group was significantly lower than those in other two groups. FFSirt 2 and Sirt 2 (HCG day ) were negatively correlated with age (r=-0.35, r=-0.19), but there were no value of them for assessing DOR (diminished ovarian reserve). The level of bSirt2 in (cumulative) pregnant group was significantly higher (r=0.24, P=0.00). Conclusions: This was the first study to show that FFSirt2 and Sirt2 (HCG day) might be negatively correlated with age and antral follicle count (AFC). bSirt2 could predict cumulative pregnancy outcome together with anti-Mullerian hormone (AMH), AFC and age.

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