Fernanda Freire
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Cristiane Aparecida Pereira
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Luciane Dias Oliveira
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Juliana Campos Junqueira
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Antonio Olavo Cardoso Jorge
<p><strong>Objective</strong>: The aim of this study was to identify the slime production and evaluate the effects of <em>Rosmarinus officinalis</em> (rosemary) and <em>Syzygium cumini</em> (jambolan) glycolic extracts, and 0.12% chlorhexidine (CHX) in biofilms formed by strains of coagulase-positive <em>Staphylococcus</em> - CPS and coagulase negative <em>Staphylococcus</em> - CNS isolated from the oral cavity. <strong>Material</strong> <strong>and</strong> <strong>Methods</strong>: Slime production was evaluated by two methods: the color of colony presented in Congo red agar, and through the amount of slime adhered to polystyrene. Biofilms were grown in acrylic resin discs immersed in broth, inoculated with microbial suspension (10<sup>6</sup> cells/ml) and incubated at 37°C/48 h. After formation, the biofilms were exposed for 5 minutes to glycol extracts, CHX or saline solution. The viability of biofilms was determined by counting the colony-forming units per milliliter (CFU/ml) in agar, and analyzed statistically by Tukey test (p <0.05). <strong>Results</strong>: The strains <em>S. aureus</em>, <em>S. schleiferi</em> and <em>S. epidermidis</em> obtained the highest values of slime adhered to polystyrene. <em>R. officinalis</em> promoted reductions ranging from 12.1% to 78.7% in biofilms formed by isolates of CPS, and 9.2% to 73.7% in the biofilms of CNS. <em>S. cumini</em> reduced 12% to 55.7% in biofilms of CPS, and 7.9% to 71.5% in biofilms of CNS. With exception of <em>S. saprophyticus</em>, glycol extracts produced significant reductions in biofilms. For five isolates studied, <em>R. officinalis</em> produced greater reductions than CHX. <strong>Conclusion</strong>:<em> R. officinalis</em> and <em>S. cumini</em> showed effective antibiofilm activity against isolates that showed slime production.</p><p><strong> </strong></p><p><strong>Keywords</strong></p><p>Biofilm; <em>Rosmarinus officinalis; </em>Slime; <em>Staphylococcus;</em> <em>Syzygium cumini</em>.</p>